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The map label for this gene is mscL

Identifier: 116496010

GI number: 116496010

Start: 2560111

End: 2560482

Strand: Direct

Name: mscL

Synonym: LSEI_2579

Alternate gene names: 116496010

Gene position: 2560111-2560482 (Clockwise)

Preceding gene: 116496009

Following gene: 116496012

Centisome position: 88.42

GC content: 41.4

Gene sequence:

>372_bases
ATGTTAAAAGAATTTCAGAAATTTATCATGCGAGGTAACGTGCTAGACTTAGCCGTTGGGGTCATCATCGGCAGTGCCTT
CACTGGTTTAGTCACCTCTTTAACTAAAAACCTTATCAACCCGATTTTATCCATGTTTGCGGGCAAAGCTGACCTCAGCG
GGCTTTATTTCACGATATTGGGCGCCAAGTTTACATATGGGAATTTTATCAATGACGTGCTCAACTTCTTGATTATTGCT
TTTGTAGTCTTTCTGCTAGTCAAAGGTATTAACCGCATTTTACCGTCAAAACCGGCCAAACCAGCTGGTCCGACACAAGA
AGAATTGCTAACAGAAATTCGCGATCTGTTAAAGCAAGATCAACAGGTCTAA

Upstream 100 bases:

>100_bases
CGATTGCTGAATTCTTACGGGTTTGGCATCTGGGATAAGCCGCAGAGATGCAGCATAAGTGTTGATTTCAGTATACTAAT
TTTGAAGGGAGTTACTGTTT

Downstream 100 bases:

>100_bases
TAACCTAAAATAATCATCTTGTACAACGCTTTATCCCATAAATATAAAAAGTGGCCTCGTTGCAGTATTTACGCGGGGCC
ACTTTTTATATGAATAATTG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 123; Mature: 123

Protein sequence:

>123_residues
MLKEFQKFIMRGNVLDLAVGVIIGSAFTGLVTSLTKNLINPILSMFAGKADLSGLYFTILGAKFTYGNFINDVLNFLIIA
FVVFLLVKGINRILPSKPAKPAGPTQEELLTEIRDLLKQDQQV

Sequences:

>Translated_123_residues
MLKEFQKFIMRGNVLDLAVGVIIGSAFTGLVTSLTKNLINPILSMFAGKADLSGLYFTILGAKFTYGNFINDVLNFLIIA
FVVFLLVKGINRILPSKPAKPAGPTQEELLTEIRDLLKQDQQV
>Mature_123_residues
MLKEFQKFIMRGNVLDLAVGVIIGSAFTGLVTSLTKNLINPILSMFAGKADLSGLYFTILGAKFTYGNFINDVLNFLIIA
FVVFLLVKGINRILPSKPAKPAGPTQEELLTEIRDLLKQDQQV

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=129, Percent_Identity=47.2868217054264, Blast_Score=105, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_LACC3 (Q034S0)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_807744.1
- ProteinModelPortal:   Q034S0
- SMR:   Q034S0
- STRING:   Q034S0
- GeneID:   4421130
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_2579
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   IQLLTEI
- PhylomeDB:   Q034S0
- ProtClustDB:   CLSK2318438
- BioCyc:   LCAS321967:LSEI_2579-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- InterPro:   IPR015089
- Gene3D:   G3DSA:1.20.5.220
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 13531; Mature: 13531

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1556b82c)-; HASH(0x16397cb8)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEFQKFIMRGNVLDLAVGVIIGSAFTGLVTSLTKNLINPILSMFAGKADLSGLYFTIL
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
GAKFTYGNFINDVLNFLIIAFVVFLLVKGINRILPSKPAKPAGPTQEELLTEIRDLLKQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
QQV
CCH
>Mature Secondary Structure
MLKEFQKFIMRGNVLDLAVGVIIGSAFTGLVTSLTKNLINPILSMFAGKADLSGLYFTIL
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
GAKFTYGNFINDVLNFLIIAFVVFLLVKGINRILPSKPAKPAGPTQEELLTEIRDLLKQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
QQV
CCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA