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The map label for this gene is nylA [H]

Identifier: 116495734

GI number: 116495734

Start: 2282540

End: 2284003

Strand: Direct

Name: nylA [H]

Synonym: LSEI_2282

Alternate gene names: 116495734

Gene position: 2282540-2284003 (Clockwise)

Preceding gene: 116495729

Following gene: 116495735

Centisome position: 78.84

GC content: 54.03

Gene sequence:

>1464_bases
ATGCAAAAATCTGCACTTGATCAAGCCGCTGCTATTCGCGCCGGAGTGCTGTCTCCAGCAGAATTGATTGATAGTACTCG
TGAGAAAATGACGGCATTAAACCCAGCGCTCAATGCCATTACCTGGACCCGTTTTGAAGCAGCCAAGCAGGACGCCAATC
AACTGGAAGATCACGGTCAACCGTTTTTCGGCGTGCCGTTGATCCTTAAAGGCTTGGGTCAAGACTTTGCTGGCGCTCCA
GCAACTTTTGGCAGTCGCTTCTTCGCCAAAGCCAAGTCCGAACACACCAATAACTTTGTTAAGGCCTTGCAACGGCTAGG
CTTCATCATCGTTGGTCAAAGTAATGTACCGGAATTTGGTTTTAAAAACATCACAGATGCAGAACTTTATGGCCCCGCCC
GTAATCCGTGGAACCGCGACTATTCGCCAGGTGGCTCTTCCGGTGGCGCAGCAGCGGTTGTGGCTTCAGGTATTAGTCCG
CTGGCAACCGGTTCTGATGGTGGCGGTTCGATCCGCATTCCCGCTTCTTTTTCCGGCTTGATCGGTTTGAAGCCAACCCG
TGGCCGCGTGCCAACCGGACCTGGTGAGTGGCGTGGCTGGCAAGGAGCATCCATCAACTTCGCCTTGACGAAAACCATGG
CCGATACAGCGGCGCTACTTCGTGGGCTTGCCACAACCCAGCTAGCTGCTCCCTTCATCGCGCCCCCATTACGCTTAGAT
CTTGTTGGCGATGCACGACCACTGAAGATTGCTTACACCACAACATCGCCTGTTGGCACCCCTGTCAGTGACACCGCTGT
TAAAGCCGTCGAAACCGCGGCGGATGCGTTGCGAGCAGCAGGACACCAAGTCGTTGCGGCTGCCCCTGATGTTGACGGTA
CTGCTTTAATGAAGGCGTACTACATGATGAACGGCGGCGAAACTGCGGCCATGTTTCAGGCATTTGCCGCCCAAACTGGC
AAGCAGGCCACCGCTGCCGACATGGAGTTGATAACTTGGGTGATCTATCAGGCTGGCTTGCACACCTCTGCAGCAGACTA
CAGCCTCTCACTTGGTGCTTGGGATCGCGCAGCAGAGGCTTACAGCCATTTTCATGAGAGCTACGACTTGCTGCTGAGTC
CAACAACAGCCAAAACAGCGCCCCGCATCGATGCCGTTTTACAATCGCCGGCCATCGTTGCTAAAATGCAACACGCCGCT
GAGCTGGCACCGCAGGAACAACAAGAGCTGATTTGGGATCTTTTTGAACCCAGTCTTGAATATTCGCCATTCACGCAACA
AGCCAATCTAACCGGTGCGCCCGCAATCAGCTTGCCGACCGCCATCTCACCTGAAGGCTTACCACTGGGCATCCAATTCA
CCGCCGCCAAAGGCCGTGAAGATCAGCTCCTGCGCATCGGCTACTGGTTTGAACAACAAGGCTTGTTGAAAATGTTACCT
GCATCGCTAAAGGAGAAGATCTAA

Upstream 100 bases:

>100_bases
GGGCAATTAACTTTAACAAAAGGTTACCGTGAATGTAAGATAGTTGTCAAAGATAACCATAGTAAGGAAAGCCTAAAAAT
TTTTAAAGGGGAGGTTCATG

Downstream 100 bases:

>100_bases
TGGGCTCACTTGCTGAATGGTACCAACGCATTCCCACGCCTGATGATTTAACCCGAGTCGAATCACTTTTTGCTAACATG
CAGGCACAATTCCCGCAACT

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MQKSALDQAAAIRAGVLSPAELIDSTREKMTALNPALNAITWTRFEAAKQDANQLEDHGQPFFGVPLILKGLGQDFAGAP
ATFGSRFFAKAKSEHTNNFVKALQRLGFIIVGQSNVPEFGFKNITDAELYGPARNPWNRDYSPGGSSGGAAAVVASGISP
LATGSDGGGSIRIPASFSGLIGLKPTRGRVPTGPGEWRGWQGASINFALTKTMADTAALLRGLATTQLAAPFIAPPLRLD
LVGDARPLKIAYTTTSPVGTPVSDTAVKAVETAADALRAAGHQVVAAAPDVDGTALMKAYYMMNGGETAAMFQAFAAQTG
KQATAADMELITWVIYQAGLHTSAADYSLSLGAWDRAAEAYSHFHESYDLLLSPTTAKTAPRIDAVLQSPAIVAKMQHAA
ELAPQEQQELIWDLFEPSLEYSPFTQQANLTGAPAISLPTAISPEGLPLGIQFTAAKGREDQLLRIGYWFEQQGLLKMLP
ASLKEKI

Sequences:

>Translated_487_residues
MQKSALDQAAAIRAGVLSPAELIDSTREKMTALNPALNAITWTRFEAAKQDANQLEDHGQPFFGVPLILKGLGQDFAGAP
ATFGSRFFAKAKSEHTNNFVKALQRLGFIIVGQSNVPEFGFKNITDAELYGPARNPWNRDYSPGGSSGGAAAVVASGISP
LATGSDGGGSIRIPASFSGLIGLKPTRGRVPTGPGEWRGWQGASINFALTKTMADTAALLRGLATTQLAAPFIAPPLRLD
LVGDARPLKIAYTTTSPVGTPVSDTAVKAVETAADALRAAGHQVVAAAPDVDGTALMKAYYMMNGGETAAMFQAFAAQTG
KQATAADMELITWVIYQAGLHTSAADYSLSLGAWDRAAEAYSHFHESYDLLLSPTTAKTAPRIDAVLQSPAIVAKMQHAA
ELAPQEQQELIWDLFEPSLEYSPFTQQANLTGAPAISLPTAISPEGLPLGIQFTAAKGREDQLLRIGYWFEQQGLLKMLP
ASLKEKI
>Mature_487_residues
MQKSALDQAAAIRAGVLSPAELIDSTREKMTALNPALNAITWTRFEAAKQDANQLEDHGQPFFGVPLILKGLGQDFAGAP
ATFGSRFFAKAKSEHTNNFVKALQRLGFIIVGQSNVPEFGFKNITDAELYGPARNPWNRDYSPGGSSGGAAAVVASGISP
LATGSDGGGSIRIPASFSGLIGLKPTRGRVPTGPGEWRGWQGASINFALTKTMADTAALLRGLATTQLAAPFIAPPLRLD
LVGDARPLKIAYTTTSPVGTPVSDTAVKAVETAADALRAAGHQVVAAAPDVDGTALMKAYYMMNGGETAAMFQAFAAQTG
KQATAADMELITWVIYQAGLHTSAADYSLSLGAWDRAAEAYSHFHESYDLLLSPTTAKTAPRIDAVLQSPAIVAKMQHAA
ELAPQEQQELIWDLFEPSLEYSPFTQQANLTGAPAISLPTAISPEGLPLGIQFTAAKGREDQLLRIGYWFEQQGLLKMLP
ASLKEKI

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=509, Percent_Identity=24.950884086444, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI222831590, Length=529, Percent_Identity=24.5746691871456, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI166795287, Length=279, Percent_Identity=29.7491039426523, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17556264, Length=268, Percent_Identity=33.2089552238806, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17537465, Length=502, Percent_Identity=25.0996015936255, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=322, Percent_Identity=27.0186335403727, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=294, Percent_Identity=27.891156462585, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=173, Percent_Identity=34.6820809248555, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI32565337, Length=267, Percent_Identity=28.0898876404494, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556276, Length=265, Percent_Identity=29.811320754717, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=265, Percent_Identity=29.811320754717, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=425, Percent_Identity=25.1764705882353, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320448, Length=187, Percent_Identity=32.0855614973262, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21356731, Length=493, Percent_Identity=24.1379310344828, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI45550774, Length=239, Percent_Identity=30.9623430962343, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24648435, Length=239, Percent_Identity=30.9623430962343, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24648437, Length=239, Percent_Identity=30.9623430962343, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24644968, Length=204, Percent_Identity=31.3725490196078, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648113, Length=482, Percent_Identity=25.3112033195021, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652985, Length=502, Percent_Identity=23.9043824701195, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19922090, Length=502, Percent_Identity=23.9043824701195, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24652981, Length=502, Percent_Identity=23.9043824701195, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24652983, Length=502, Percent_Identity=23.9043824701195, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI161078093, Length=189, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648441, Length=170, Percent_Identity=33.5294117647059, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648439, Length=170, Percent_Identity=33.5294117647059, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 51527; Mature: 51527

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKSALDQAAAIRAGVLSPAELIDSTREKMTALNPALNAITWTRFEAAKQDANQLEDHGQ
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PFFGVPLILKGLGQDFAGAPATFGSRFFAKAKSEHTNNFVKALQRLGFIIVGQSNVPEFG
CCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCC
FKNITDAELYGPARNPWNRDYSPGGSSGGAAAVVASGISPLATGSDGGGSIRIPASFSGL
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEECCCCCCC
IGLKPTRGRVPTGPGEWRGWQGASINFALTKTMADTAALLRGLATTQLAAPFIAPPLRLD
EECCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCHHCCCCCEEE
LVGDARPLKIAYTTTSPVGTPVSDTAVKAVETAADALRAAGHQVVAAAPDVDGTALMKAY
EECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
YMMNGGETAAMFQAFAAQTGKQATAADMELITWVIYQAGLHTSAADYSLSLGAWDRAAEA
HHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHH
YSHFHESYDLLLSPTTAKTAPRIDAVLQSPAIVAKMQHAAELAPQEQQELIWDLFEPSLE
HHHHHHCCCEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
YSPFTQQANLTGAPAISLPTAISPEGLPLGIQFTAAKGREDQLLRIGYWFEQQGLLKMLP
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEEEECCCCHHHHCC
ASLKEKI
HHHHHCC
>Mature Secondary Structure
MQKSALDQAAAIRAGVLSPAELIDSTREKMTALNPALNAITWTRFEAAKQDANQLEDHGQ
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PFFGVPLILKGLGQDFAGAPATFGSRFFAKAKSEHTNNFVKALQRLGFIIVGQSNVPEFG
CCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCC
FKNITDAELYGPARNPWNRDYSPGGSSGGAAAVVASGISPLATGSDGGGSIRIPASFSGL
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEECCCCCCC
IGLKPTRGRVPTGPGEWRGWQGASINFALTKTMADTAALLRGLATTQLAAPFIAPPLRLD
EECCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCHHCCCCCEEE
LVGDARPLKIAYTTTSPVGTPVSDTAVKAVETAADALRAAGHQVVAAAPDVDGTALMKAY
EECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
YMMNGGETAAMFQAFAAQTGKQATAADMELITWVIYQAGLHTSAADYSLSLGAWDRAAEA
HHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHH
YSHFHESYDLLLSPTTAKTAPRIDAVLQSPAIVAKMQHAAELAPQEQQELIWDLFEPSLE
HHHHHHCCCEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
YSPFTQQANLTGAPAISLPTAISPEGLPLGIQFTAAKGREDQLLRIGYWFEQQGLLKMLP
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEEEECCCCHHHHCC
ASLKEKI
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]