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The map label for this gene is ackA [H]

Identifier: 116495627

GI number: 116495627

Start: 2162953

End: 2164158

Strand: Direct

Name: ackA [H]

Synonym: LSEI_2172

Alternate gene names: 116495627

Gene position: 2162953-2164158 (Clockwise)

Preceding gene: 116495626

Following gene: 116495628

Centisome position: 74.71

GC content: 49.34

Gene sequence:

>1206_bases
ATGGCAAAAGTTCTCTCCATCAACGCTGGCAGCTCCTCACTCAAATGGAAGCTGTTCGAGATGCCAAGTGAGCGCCAGCT
TGCAGAAGGCGCGACTGACATCCTGAAACAATCAACCGTCAAGATCAAGTATGGTACTGATCAAGTTTACGAAAGCGCTA
CTCCAATTTGCAATTATCGTGACGCTGTTGCCAACCTGCTCTCTAACCTGCAAACACTCGGACTCGTTCAAAATTTGGAT
GAAATTACCGGCATTGGCCATCGCGTGGTAGCTGGCGGGGAATTATTTTCCGCCCCAATTGTCATTGATGATCGTGTTCT
GGCGCAGATTCGTGCTCTGCGTGATTATGCTCCGCTCCACAATCCGGTTGAAGCCGACTGTATCGCCATCTTTCGCAAAA
TGATGCCTTGGGCACTTGAAGTCGCAGTATTTGATACCGCATTTCATCAAACCATGAAACCAGTCAATTACCTTTACAGT
ATTCCGTATGAGTATTATCAAAAGTATGGTGTCCGTAAATACGGTGCGCATGGAACCAGCGTTCGCTATGTGAGTGCTCA
CGCCGCCAAAATGCTTCGCAAACCATTGGCACAAATGCGAATGATTGTGATGCATCTTGGGGCGGGTTCAAGCATCACGG
CCATCCAAAACGGGCAATCGGTTGACACATCAATGGGCTTTACCCCTTTGTCCGGTGTCACGATGGGCACGCGTTCAGGC
GATGTTGATGTGTCACTCATCGGCTATCTCATGAAGAAACTTGCCATCACCGATGTCGATCAAATGATTGATATCTTAAA
TACAGAGTCAGGCCTACGCGGGATCTCCGGCATTAGTCATGACGTTCGCGACCTTGAAGCCGCTGCGCCGACTAACCCGC
GAGCCAAACTGGCGCTTGATATCTTTGTGAACCGCATTGTTAAATATGTCGGTGCTTATGCCGCCTTGATGGACGGTGTC
GATGTCCTAGTCTTCACAGCTGGCATCGGCGAACACAGCAGTGACATTCGGGCACGCGTCATGCAATCTTTAGATTATCT
CGGCGCGCGGATTGATGCGAGCTTAAACACTCAAATCCACGATAACGCAGGTGACATTACTGCAGCTGATTCAAAGGTCA
AAACACTGGTCATCCCCACAAATGAAGAATTGATGATCGTCCGCGACGTCATGTCCTTAAGCCAAGCAGTGGCGCAAGCG
GAATAG

Upstream 100 bases:

>100_bases
AAAAATTGAAATTGTAATATTTCAGCCCGACGTTTTCACAAACATTGGGCTTTTTCAGTGGTATGATGTATGTAAAGCAA
AAGTGAAAGAGTGATCAAAC

Downstream 100 bases:

>100_bases
TGGTAGGCGTTCGCCAGCCCAGAAACAGAACTGGCGAACGCGCTACGATCGAGCATGAGCAAGCCCGGTTAGAAATCGAC
GCGTTATAGGCTTCCCTGCT

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MAKVLSINAGSSSLKWKLFEMPSERQLAEGATDILKQSTVKIKYGTDQVYESATPICNYRDAVANLLSNLQTLGLVQNLD
EITGIGHRVVAGGELFSAPIVIDDRVLAQIRALRDYAPLHNPVEADCIAIFRKMMPWALEVAVFDTAFHQTMKPVNYLYS
IPYEYYQKYGVRKYGAHGTSVRYVSAHAAKMLRKPLAQMRMIVMHLGAGSSITAIQNGQSVDTSMGFTPLSGVTMGTRSG
DVDVSLIGYLMKKLAITDVDQMIDILNTESGLRGISGISHDVRDLEAAAPTNPRAKLALDIFVNRIVKYVGAYAALMDGV
DVLVFTAGIGEHSSDIRARVMQSLDYLGARIDASLNTQIHDNAGDITAADSKVKTLVIPTNEELMIVRDVMSLSQAVAQA
E

Sequences:

>Translated_401_residues
MAKVLSINAGSSSLKWKLFEMPSERQLAEGATDILKQSTVKIKYGTDQVYESATPICNYRDAVANLLSNLQTLGLVQNLD
EITGIGHRVVAGGELFSAPIVIDDRVLAQIRALRDYAPLHNPVEADCIAIFRKMMPWALEVAVFDTAFHQTMKPVNYLYS
IPYEYYQKYGVRKYGAHGTSVRYVSAHAAKMLRKPLAQMRMIVMHLGAGSSITAIQNGQSVDTSMGFTPLSGVTMGTRSG
DVDVSLIGYLMKKLAITDVDQMIDILNTESGLRGISGISHDVRDLEAAAPTNPRAKLALDIFVNRIVKYVGAYAALMDGV
DVLVFTAGIGEHSSDIRARVMQSLDYLGARIDASLNTQIHDNAGDITAADSKVKTLVIPTNEELMIVRDVMSLSQAVAQA
E
>Mature_400_residues
AKVLSINAGSSSLKWKLFEMPSERQLAEGATDILKQSTVKIKYGTDQVYESATPICNYRDAVANLLSNLQTLGLVQNLDE
ITGIGHRVVAGGELFSAPIVIDDRVLAQIRALRDYAPLHNPVEADCIAIFRKMMPWALEVAVFDTAFHQTMKPVNYLYSI
PYEYYQKYGVRKYGAHGTSVRYVSAHAAKMLRKPLAQMRMIVMHLGAGSSITAIQNGQSVDTSMGFTPLSGVTMGTRSGD
VDVSLIGYLMKKLAITDVDQMIDILNTESGLRGISGISHDVRDLEAAAPTNPRAKLALDIFVNRIVKYVGAYAALMDGVD
VLVFTAGIGEHSSDIRARVMQSLDYLGARIDASLNTQIHDNAGDITAADSKVKTLVIPTNEELMIVRDVMSLSQAVAQAE

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=395, Percent_Identity=40.253164556962, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI145693184, Length=406, Percent_Identity=40.1477832512315, Blast_Score=280, Evalue=1e-76,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43690; Mature: 43559

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVLSINAGSSSLKWKLFEMPSERQLAEGATDILKQSTVKIKYGTDQVYESATPICNYR
CCEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCHHHHHCCCCCCCHH
DAVANLLSNLQTLGLVQNLDEITGIGHRVVAGGELFSAPIVIDDRVLAQIRALRDYAPLH
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEECCEEECHHHHHHHHHHHHHCCCC
NPVEADCIAIFRKMMPWALEVAVFDTAFHQTMKPVNYLYSIPYEYYQKYGVRKYGAHGTS
CCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCE
VRYVSAHAAKMLRKPLAQMRMIVMHLGAGSSITAIQNGQSVDTSMGFTPLSGVTMGTRSG
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEECCCCC
DVDVSLIGYLMKKLAITDVDQMIDILNTESGLRGISGISHDVRDLEAAAPTNPRAKLALD
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHCCCCCCCHHHHHH
IFVNRIVKYVGAYAALMDGVDVLVFTAGIGEHSSDIRARVMQSLDYLGARIDASLNTQIH
HHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEE
DNAGDITAADSKVKTLVIPTNEELMIVRDVMSLSQAVAQAE
CCCCCEEECCCCEEEEEEECCCCEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKVLSINAGSSSLKWKLFEMPSERQLAEGATDILKQSTVKIKYGTDQVYESATPICNYR
CEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCHHHHHCCCCCCCHH
DAVANLLSNLQTLGLVQNLDEITGIGHRVVAGGELFSAPIVIDDRVLAQIRALRDYAPLH
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEECCEEECHHHHHHHHHHHHHCCCC
NPVEADCIAIFRKMMPWALEVAVFDTAFHQTMKPVNYLYSIPYEYYQKYGVRKYGAHGTS
CCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCE
VRYVSAHAAKMLRKPLAQMRMIVMHLGAGSSITAIQNGQSVDTSMGFTPLSGVTMGTRSG
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEECCCCC
DVDVSLIGYLMKKLAITDVDQMIDILNTESGLRGISGISHDVRDLEAAAPTNPRAKLALD
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHCCCCCCCHHHHHH
IFVNRIVKYVGAYAALMDGVDVLVFTAGIGEHSSDIRARVMQSLDYLGARIDASLNTQIH
HHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEE
DNAGDITAADSKVKTLVIPTNEELMIVRDVMSLSQAVAQAE
CCCCCEEECCCCEEEEEEECCCCEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA