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The map label for this gene is tig

Identifier: 116494826

GI number: 116494826

Start: 1323031

End: 1324368

Strand: Direct

Name: tig

Synonym: LSEI_1338

Alternate gene names: 116494826

Gene position: 1323031-1324368 (Clockwise)

Preceding gene: 116494825

Following gene: 116494828

Centisome position: 45.7

GC content: 44.99

Gene sequence:

>1338_bases
ATGTCTGCAAAATGGGAAAAAAAGGGCACCAATGATGGTGAGCTCACCTTTGAAATCGATTTACCACAAATTCAACAAGG
TTTAGACCAAGCGTTTCAACGTGTTCGCAAGAACTTGACGGTTCCTGGGTTCCGTAAAGGCAAAGTTAGCCGCACCGTGT
TCAAACGGATGTATGGCGACGCTGCACTTTATGAAGATGCTTTGAACATCTTGCTGCCAGACGCTTATGAAGCAGCCGTT
AAGGAATCCGGTATTGATCCGGTTGACCAGCCGCAAATCAACGTTGATTCTATGGACGAAGGCAAACCTTGGGTCATCAA
GGCAACCGTTACGGTTAAGCCAGAAGTCACACTTGGCCAGTATAAGGGTCTTGAAGTGCCAAAGCAGAACGTTGAAGTTT
CTGCAAAGGATATTGACGCTGAATTGGAAAAGCGTCGCGAACAGCAGGCTGAACTCGTTGTTAAAGACGACAAAGCAGCT
GAAAATGGCGATACCGTTGTGATCGACTATGTCGGGACAATTGACGGGACGGAATTTGACGGCGGCTCTTCCAAGAACTA
TAGCTTGGAATTAGGGTCAAACTCATTCATTCCTGGCTTTGAAGAACAACTTGTTGGTCACAAATCTGGTGATGAAGTGA
CCGTTAATGTCACCTTCCCAGAAGACTACAAGGCAGCTGATTTAGCTGGTAAAGCAGCCGAATTCAAGACCACGATCCAT
GAAGTCAAAGTTAAGGAATTGCCTGCATTGGATGACGACTTTGCGAAGGATCTTGATGATGATGTTGATACCCTTGATGA
ATTGAAAGCAAAAATCAAGAAGGAATTGACCGATCAGCGTGAGGAAGCTGCAAAGAATGCTGTTCAGGAAGCTGCCATCA
AGGAAGCTACTGACAATGCGACGATCAAAGAAGTGCCAAATGCGATGATCGAGCAAGAAGTGCATAATCAGATGGATCAA
TATCTTGGCAACATGCAACGCCAAGGGATCTCACCAAAAATGTACTATCAATTAACTGGTACATCAGAGGATGATTTGCA
CAAGCAGTTTGAGGCTGATGCAGCTACGCGTGTTCGGACAAACTTAGTCCTTGAAGCCATCGTGAAGGCCGAAGATATTC
AGCCAACCGAAGACCAAGTGAACGAAGAAGTCAAAAACTTGGCTTCTGAATACAACATGGACGAAAAGGCCGTTCGTAAG
GCTTTGAGTGAAGATATGCTCAAACATGATATTGGTGTCAAACAAGCCATTGATATCATCACGGACAGTGCAAAAGAAGT
CGAATCAGCTAAAGATGATGCTGACAAAGAAGCTTCTGACGCCAAGGCTGACAAATAG

Upstream 100 bases:

>100_bases
TCGCCTTTTTTGGCCACAACATTTACATTCAAGTGGGTTTCGGGTATCATACATTAAGTATGCAATGTGAAATTGCGAGA
CTAATTGTCGGAGGTACAGC

Downstream 100 bases:

>100_bases
TTTGCCGTTTCCACGTAAAAGAGACGATCCCCGATCGTCTCTTTTGTTGTATGAACGCGTTTTTAGCGTATACTTGACGT
TATTCTTTTAGCAGAAAGAA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSAKWEKKGTNDGELTFEIDLPQIQQGLDQAFQRVRKNLTVPGFRKGKVSRTVFKRMYGDAALYEDALNILLPDAYEAAV
KESGIDPVDQPQINVDSMDEGKPWVIKATVTVKPEVTLGQYKGLEVPKQNVEVSAKDIDAELEKRREQQAELVVKDDKAA
ENGDTVVIDYVGTIDGTEFDGGSSKNYSLELGSNSFIPGFEEQLVGHKSGDEVTVNVTFPEDYKAADLAGKAAEFKTTIH
EVKVKELPALDDDFAKDLDDDVDTLDELKAKIKKELTDQREEAAKNAVQEAAIKEATDNATIKEVPNAMIEQEVHNQMDQ
YLGNMQRQGISPKMYYQLTGTSEDDLHKQFEADAATRVRTNLVLEAIVKAEDIQPTEDQVNEEVKNLASEYNMDEKAVRK
ALSEDMLKHDIGVKQAIDIITDSAKEVESAKDDADKEASDAKADK

Sequences:

>Translated_445_residues
MSAKWEKKGTNDGELTFEIDLPQIQQGLDQAFQRVRKNLTVPGFRKGKVSRTVFKRMYGDAALYEDALNILLPDAYEAAV
KESGIDPVDQPQINVDSMDEGKPWVIKATVTVKPEVTLGQYKGLEVPKQNVEVSAKDIDAELEKRREQQAELVVKDDKAA
ENGDTVVIDYVGTIDGTEFDGGSSKNYSLELGSNSFIPGFEEQLVGHKSGDEVTVNVTFPEDYKAADLAGKAAEFKTTIH
EVKVKELPALDDDFAKDLDDDVDTLDELKAKIKKELTDQREEAAKNAVQEAAIKEATDNATIKEVPNAMIEQEVHNQMDQ
YLGNMQRQGISPKMYYQLTGTSEDDLHKQFEADAATRVRTNLVLEAIVKAEDIQPTEDQVNEEVKNLASEYNMDEKAVRK
ALSEDMLKHDIGVKQAIDIITDSAKEVESAKDDADKEASDAKADK
>Mature_444_residues
SAKWEKKGTNDGELTFEIDLPQIQQGLDQAFQRVRKNLTVPGFRKGKVSRTVFKRMYGDAALYEDALNILLPDAYEAAVK
ESGIDPVDQPQINVDSMDEGKPWVIKATVTVKPEVTLGQYKGLEVPKQNVEVSAKDIDAELEKRREQQAELVVKDDKAAE
NGDTVVIDYVGTIDGTEFDGGSSKNYSLELGSNSFIPGFEEQLVGHKSGDEVTVNVTFPEDYKAADLAGKAAEFKTTIHE
VKVKELPALDDDFAKDLDDDVDTLDELKAKIKKELTDQREEAAKNAVQEAAIKEATDNATIKEVPNAMIEQEVHNQMDQY
LGNMQRQGISPKMYYQLTGTSEDDLHKQFEADAATRVRTNLVLEAIVKAEDIQPTEDQVNEEVKNLASEYNMDEKAVRKA
LSEDMLKHDIGVKQAIDIITDSAKEVESAKDDADKEASDAKADK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=381, Percent_Identity=31.758530183727, Blast_Score=183, Evalue=2e-47,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_LACC3 (Q039K4)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806560.1
- ProteinModelPortal:   Q039K4
- STRING:   Q039K4
- GeneID:   4419426
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_1338
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   Q039K4
- ProtClustDB:   PRK01490
- BioCyc:   LCAS321967:LSEI_1338-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PANTHER:   PTHR10516
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 49379; Mature: 49248

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKWEKKGTNDGELTFEIDLPQIQQGLDQAFQRVRKNLTVPGFRKGKVSRTVFKRMYGD
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
AALYEDALNILLPDAYEAAVKESGIDPVDQPQINVDSMDEGKPWVIKATVTVKPEVTLGQ
HHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCEECCC
YKGLEVPKQNVEVSAKDIDAELEKRREQQAELVVKDDKAAENGDTVVIDYVGTIDGTEFD
CCCCCCCCCCCEECHHHHHHHHHHHHHHHHCEEEECCCCCCCCCEEEEEEEECCCCCCCC
GGSSKNYSLELGSNSFIPGFEEQLVGHKSGDEVTVNVTFPEDYKAADLAGKAAEFKTTIH
CCCCCCEEEEECCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCHHHHCCCHHHHHHHHH
EVKVKELPALDDDFAKDLDDDVDTLDELKAKIKKELTDQREEAAKNAVQEAAIKEATDNA
HHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TIKEVPNAMIEQEVHNQMDQYLGNMQRQGISPKMYYQLTGTSEDDLHKQFEADAATRVRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
NLVLEAIVKAEDIQPTEDQVNEEVKNLASEYNMDEKAVRKALSEDMLKHDIGVKQAIDII
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
TDSAKEVESAKDDADKEASDAKADK
HCCHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAKWEKKGTNDGELTFEIDLPQIQQGLDQAFQRVRKNLTVPGFRKGKVSRTVFKRMYGD
CCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
AALYEDALNILLPDAYEAAVKESGIDPVDQPQINVDSMDEGKPWVIKATVTVKPEVTLGQ
HHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCEECCC
YKGLEVPKQNVEVSAKDIDAELEKRREQQAELVVKDDKAAENGDTVVIDYVGTIDGTEFD
CCCCCCCCCCCEECHHHHHHHHHHHHHHHHCEEEECCCCCCCCCEEEEEEEECCCCCCCC
GGSSKNYSLELGSNSFIPGFEEQLVGHKSGDEVTVNVTFPEDYKAADLAGKAAEFKTTIH
CCCCCCEEEEECCCCCCCCHHHHHCCCCCCCEEEEEEECCCCCCHHHHCCCHHHHHHHHH
EVKVKELPALDDDFAKDLDDDVDTLDELKAKIKKELTDQREEAAKNAVQEAAIKEATDNA
HHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TIKEVPNAMIEQEVHNQMDQYLGNMQRQGISPKMYYQLTGTSEDDLHKQFEADAATRVRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
NLVLEAIVKAEDIQPTEDQVNEEVKNLASEYNMDEKAVRKALSEDMLKHDIGVKQAIDII
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
TDSAKEVESAKDDADKEASDAKADK
HCCHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA