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The map label for this gene is cspB [H]

Identifier: 116494772

GI number: 116494772

Start: 1260747

End: 1260968

Strand: Direct

Name: cspB [H]

Synonym: LSEI_1281

Alternate gene names: 116494772

Gene position: 1260747-1260968 (Clockwise)

Preceding gene: 116494771

Following gene: 116494774

Centisome position: 43.55

GC content: 46.85

Gene sequence:

>222_bases
ATGCAAGGACGCGTTAAAAACTTTAACGTTGACAAAGGCTATGGCTTTATTGAAGCTGAGGGGCAACCGGATATTTTCGT
TCACTTTTCGGCGATTAATGAACCGGGATTTAAAAGTCTGACGCCCGGTGAAACGGTTGATTTCGTTATTGTTGAAGGGC
CTCGGGGTCCACAAGCAGCGAATGTGACGGTTCATCGATCTGAAGATGGTGCTCCGCAATAG

Upstream 100 bases:

>100_bases
TCCAAAGTTGCCGCAATTGTGCAGCCGGTGTGCAGCTATCGTTGAGGCTAATTTCCCAGATGCTGTCACCAACGGGTTTG
ATAAGTAGGCAGGTGAAGAC

Downstream 100 bases:

>100_bases
ACTTTTTGATGCCCCGTTGTCGGCTGGCGATAGGGCGCTTACGGAAACTCTAGACAGTAAAAAAACACCGCGAGATCAAA
TCGCGGTGTTTTTGTATACA

Product: cold shock protein

Products: NA

Alternate protein names: Major cold shock protein [H]

Number of amino acids: Translated: 73; Mature: 73

Protein sequence:

>73_residues
MQGRVKNFNVDKGYGFIEAEGQPDIFVHFSAINEPGFKSLTPGETVDFVIVEGPRGPQAANVTVHRSEDGAPQ

Sequences:

>Translated_73_residues
MQGRVKNFNVDKGYGFIEAEGQPDIFVHFSAINEPGFKSLTPGETVDFVIVEGPRGPQAANVTVHRSEDGAPQ
>Mature_73_residues
MQGRVKNFNVDKGYGFIEAEGQPDIFVHFSAINEPGFKSLTPGETVDFVIVEGPRGPQAANVTVHRSEDGAPQ

Specific function: Binds to the pentamer sequences ATTGG and CCAAT with highest affinity in single stranded DNA, and also to other sequences. Has greater affinity for ATTGG than CCAAT. Can act as transcriptional activator of cold shock genes by recognizing putative ATTGG-bo

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=56.25, Blast_Score=72, Evalue=1e-14,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=54.6875, Blast_Score=70, Evalue=4e-14,
Organism=Escherichia coli, GI1786841, Length=63, Percent_Identity=55.5555555555556, Blast_Score=69, Evalue=5e-14,
Organism=Escherichia coli, GI1787839, Length=65, Percent_Identity=50.7692307692308, Blast_Score=68, Evalue=1e-13,
Organism=Escherichia coli, GI2367114, Length=63, Percent_Identity=52.3809523809524, Blast_Score=66, Evalue=4e-13,
Organism=Escherichia coli, GI1787834, Length=63, Percent_Identity=52.3809523809524, Blast_Score=63, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7902; Mature: 7902

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGRVKNFNVDKGYGFIEAEGQPDIFVHFSAINEPGFKSLTPGETVDFVIVEGPRGPQAA
CCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCC
NVTVHRSEDGAPQ
EEEEECCCCCCCC
>Mature Secondary Structure
MQGRVKNFNVDKGYGFIEAEGQPDIFVHFSAINEPGFKSLTPGETVDFVIVEGPRGPQAA
CCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCC
NVTVHRSEDGAPQ
EEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400185; 8969498; 9384377; 8294017; 9439003; 8755892; 8307174; 8321288; 8321289 [H]