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The map label for this gene is mraW

Identifier: 116494758

GI number: 116494758

Start: 1244749

End: 1245690

Strand: Direct

Name: mraW

Synonym: LSEI_1267

Alternate gene names: 116494758

Gene position: 1244749-1245690 (Clockwise)

Preceding gene: 116494757

Following gene: 116494759

Centisome position: 42.99

GC content: 46.07

Gene sequence:

>942_bases
ATGACAGAATTTAAACATGAAACAGTTCTATTAAAAGAAGCGACGGCGGCATTGGCGGTTAAACCTGCTGGTACTTACGT
TGATGCAACGTTAGGCCGCGGTGGCCATACTCGACAGATTCTCAATCACCTGACAACTGGTCGATTAATTGCGTTTGATC
AAGATGAGGCGGCGATTGCAACGGTCACAGCCGATTTTGGAACATTGCCAAAACAACTCACTTTGGTACATAGAAATTTT
CGAGATTTGACCGATGCATTAACGACGCTGGGAATAACTGAAGTTGATGGCATTTTATACGATCTCGGCGTTTCGTCACC
ACAATTTGATGATTCAAAGCGCGGCTTTTCCTATCGCTTTGACGCGCCACTCGATATGCGCATGGACCAGCGTCAAACGC
TCGATGCAAAAACAATTGTCAATGAATGGCCTTATGCCGATTTGGTTCGCATCTTTTCCCGATATGGCGAGGAACATTTT
TCTAAACAAATCGCTCGACGCATTGAGCAAGCACGAACAGTCCAGCCGATTACAACGACCTTCCAATTGGTTGAGTTGAT
TAAAGCAGGCATTCCAGCAAAGGCTCGGCGAACAGGCGGTCATCCAGCTAAAAAAGTTTTTCAGGCAATTCGGATCGCGG
TTAATGATGAGCTATCAGCGCTTGAGTCGTCCTTAGAACAAGCGTTGAAGTTGATCAACGTCGGGGGACGCATCAGCGTT
ATCACGTTCCAATCTTTAGAAGATCGACTCGTCAAAACGATGTTTAAAGAAGTTTCTTCGGTTCAGGATGTGCCGCGTGG
CTTGCCGGTCATTCCGGCCTCTGCACAACCGAATTATCGCCTAGTAAATCGAAAACCGATTTTACCAAGTGAAGAGGAGC
TTGCGGTGAATCACCGCGCTCATAGTGCCAAATTGAGAGTCATTGAAAAAATTCACGATTAG

Upstream 100 bases:

>100_bases
TCGAAGTTTGGGATGCCGAACGGTGGGAACAGTTTGCTGAAACCGCTGAAGAAAACTTTGATGATATTTCTGAAAATCTA
ACGGACTTTGGGTTATAGCG

Downstream 100 bases:

>100_bases
GGGGTACAATCTATGCTTGAGAGTACAGCAAGACCATTAGTGCAGCAGCCGGAAACACCGCCAATACATAAACCTGTCAC
CACACCTGCGCCCAACACAC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTEFKHETVLLKEATAALAVKPAGTYVDATLGRGGHTRQILNHLTTGRLIAFDQDEAAIATVTADFGTLPKQLTLVHRNF
RDLTDALTTLGITEVDGILYDLGVSSPQFDDSKRGFSYRFDAPLDMRMDQRQTLDAKTIVNEWPYADLVRIFSRYGEEHF
SKQIARRIEQARTVQPITTTFQLVELIKAGIPAKARRTGGHPAKKVFQAIRIAVNDELSALESSLEQALKLINVGGRISV
ITFQSLEDRLVKTMFKEVSSVQDVPRGLPVIPASAQPNYRLVNRKPILPSEEELAVNHRAHSAKLRVIEKIHD

Sequences:

>Translated_313_residues
MTEFKHETVLLKEATAALAVKPAGTYVDATLGRGGHTRQILNHLTTGRLIAFDQDEAAIATVTADFGTLPKQLTLVHRNF
RDLTDALTTLGITEVDGILYDLGVSSPQFDDSKRGFSYRFDAPLDMRMDQRQTLDAKTIVNEWPYADLVRIFSRYGEEHF
SKQIARRIEQARTVQPITTTFQLVELIKAGIPAKARRTGGHPAKKVFQAIRIAVNDELSALESSLEQALKLINVGGRISV
ITFQSLEDRLVKTMFKEVSSVQDVPRGLPVIPASAQPNYRLVNRKPILPSEEELAVNHRAHSAKLRVIEKIHD
>Mature_312_residues
TEFKHETVLLKEATAALAVKPAGTYVDATLGRGGHTRQILNHLTTGRLIAFDQDEAAIATVTADFGTLPKQLTLVHRNFR
DLTDALTTLGITEVDGILYDLGVSSPQFDDSKRGFSYRFDAPLDMRMDQRQTLDAKTIVNEWPYADLVRIFSRYGEEHFS
KQIARRIEQARTVQPITTTFQLVELIKAGIPAKARRTGGHPAKKVFQAIRIAVNDELSALESSLEQALKLINVGGRISVI
TFQSLEDRLVKTMFKEVSSVQDVPRGLPVIPASAQPNYRLVNRKPILPSEEELAVNHRAHSAKLRVIEKIHD

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=336, Percent_Identity=34.5238095238095, Blast_Score=165, Evalue=5e-41,
Organism=Homo sapiens, GI165377202, Length=187, Percent_Identity=37.9679144385027, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1786270, Length=317, Percent_Identity=42.5867507886435, Blast_Score=236, Evalue=2e-63,
Organism=Drosophila melanogaster, GI62472493, Length=334, Percent_Identity=34.7305389221557, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI28571637, Length=334, Percent_Identity=34.7305389221557, Blast_Score=163, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_LACC3 (Q039S2)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806492.1
- ProteinModelPortal:   Q039S2
- STRING:   Q039S2
- GeneID:   4419563
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_1267
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   Q039S2
- ProtClustDB:   PRK00050
- BioCyc:   LCAS321967:LSEI_1267-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34918; Mature: 34787

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: BINDING 53-53 BINDING 80-80 BINDING 101-101 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEFKHETVLLKEATAALAVKPAGTYVDATLGRGGHTRQILNHLTTGRLIAFDQDEAAIA
CCCCCCCEEHHHHHHHHEEECCCCCEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCEEE
TVTADFGTLPKQLTLVHRNFRDLTDALTTLGITEVDGILYDLGVSSPQFDDSKRGFSYRF
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
DAPLDMRMDQRQTLDAKTIVNEWPYADLVRIFSRYGEEHFSKQIARRIEQARTVQPITTT
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHH
FQLVELIKAGIPAKARRTGGHPAKKVFQAIRIAVNDELSALESSLEQALKLINVGGRISV
HHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEE
ITFQSLEDRLVKTMFKEVSSVQDVPRGLPVIPASAQPNYRLVNRKPILPSEEELAVNHRA
EEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEECCCCCCCCHHHHHHHHHH
HSAKLRVIEKIHD
HHHHHHHHHHHCC
>Mature Secondary Structure 
TEFKHETVLLKEATAALAVKPAGTYVDATLGRGGHTRQILNHLTTGRLIAFDQDEAAIA
CCCCCCEEHHHHHHHHEEECCCCCEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCEEE
TVTADFGTLPKQLTLVHRNFRDLTDALTTLGITEVDGILYDLGVSSPQFDDSKRGFSYRF
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
DAPLDMRMDQRQTLDAKTIVNEWPYADLVRIFSRYGEEHFSKQIARRIEQARTVQPITTT
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHH
FQLVELIKAGIPAKARRTGGHPAKKVFQAIRIAVNDELSALESSLEQALKLINVGGRISV
HHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEE
ITFQSLEDRLVKTMFKEVSSVQDVPRGLPVIPASAQPNYRLVNRKPILPSEEELAVNHRA
EEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEECCCCCCCCHHHHHHHHHH
HSAKLRVIEKIHD
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA