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The map label for this gene is gshAB [H]

Identifier: 116494690

GI number: 116494690

Start: 1177180

End: 1179144

Strand: Direct

Name: gshAB [H]

Synonym: LSEI_1196

Alternate gene names: 116494690

Gene position: 1177180-1179144 (Clockwise)

Preceding gene: 116494685

Following gene: 116494692

Centisome position: 40.66

GC content: 50.89

Gene sequence:

>1965_bases
ATGTTGAATCATTATTGGCAACTGATCCAGAAAAATAACTTGTTTGGCTTACGGGACGACACTCGAATCGGGGTGATTCG
GACGGTGAAGCCGGTGTTGGCGGAAGACGAGCAGCTGCCAGTGTGGGCGAATGCGAGTCGGCATGCGTTGGTGCAGCAGG
TGTCGGCTACAATGTTACAGTTGACGACACCGCTTTTAGACGATCCCAGCGAAGTTGTGGCGTATTTGCGTGCGGGACTC
AGCCGCGTCTGGGCAGCGCTGCCAGAAAACGGCAAGCTGCCGGTTTTCACCGCCGGATTGGAAAATAATCAGCGCATTCG
CATTGTTTTGCCGGAAGTGTTGTTTGAGCGGCTTTATACGGCGGAAAATTTTCAAGGGAATGGTCCTGATTATGTGACGT
ACCGGAATCAAATTTATGATTTGGTCACTGATGGGTTGATCACACAATTGCCGTTACTGACTTATCTCTTTGCCGCTTCA
CCGGCCAAAAACGGCCGGGCATTTTGGCCTGCGCCGGATCAGGAACGGCAACTGGTTCAAAAGGTCAGTGACCCTGATCA
CTTAGCGCGAACATTGGCTTTGGATGTTGCAGTGGAACTTGATCCTTATGCGGCGAGCGGTGTTACGGAACAAATGTTGA
ACTTTTTAATCGACAGTTGCTGGTTTGCTTTGAGTCAACCAGCGATTCCATTGCCCGCGGCAGCTGAGGTGCGTCTGCAT
AGTTTGGCAGAAGCACAGAAGATTGCTGCTGGCGATCCCACGGCACCGGTCGCGAGTTTGACAGCACCGATTGATGCCAT
GGCGGTCTGGCTGAATCAGGTCGGCCTCACACCGCAACGAAAACAAGCTTTTGAGTTGATGCAAAGCCGAGTCCTGAAGC
CAGAAACGACGATTGCGGGTCAGGTGGCGGCGGCTTATCATGATATTTCAGCAGCTCAAACACCGTTAGCAACGCAAGCC
CACGCAGATTTGTCAATGGAAGAAGATTTACCGGGCTTCAGTAACTTGTCAGGCAACAGTCAAGCCTTGCTTCAGGCAGT
GATTGATGGCGGGTATCAGTGGACCTTGCTGGATCGGGGACAGAACATTCTGCAGATTGCCAGTGATACTCAGCGCCATG
TGTTGATTGACGGTGCGTTGACTAGTCGCACACCTGCTAGTGCCATGGTGGTAGCAGAACATCGCCATGCCGCCAAAAAG
GTGTTAGCGGCGGCTGGGTTACCTGTTGCCAGAGGCGCCAAGTTCACACGTTGGCCTGAAGCTAAAGCTGCTTTTGAGCA
AAGCTTTGCCCGTAAAAGTATCGTCGTGAAACCTGAACAGCGTAGTCATGGGCTTGCTGTTGAGCAATTTGCGGTGCCGC
CAACTGCCAAACAATTCGCCCAAGCCTTTCACGCTGCTAACCAAGATCACGGCGTATTGGTCGAGATGATGGGGCGGGGA
ACAACGTATCATTTTACTGTTATAGGTCGCCGTGTGGTAAGTGTGTTGGAAAATGCCGCTGCCAATGTGGTTGGCGATGG
TCGTAAATCAATCAAAGAATTGATTGCGTTGAAAAATGGTAAGCGACCGAATGCACGACAGCTCAAACTTGACGAAACAG
CAAATCGGCAGTTGAAGTTACAATCGGTTACGATGAATACTGTCTTGCGGCGCGGTCAGCAGATTTTCCTTGCTTCTGCG
GCGCATCCGCAAACAGGTGGCGATATTTATGATGTTACAACCGAAATCGATCCAAGCTACAACCAACTTGCGGTTGCCGC
TGCTGATGCGCTTGAATTGCCGATTGCCGCCGTTGATATCGTGATTGATAATCTTTATGACGCCTATGCTGCTGAGCATG
AAGGCCAAGCTATCATCATCAGTGTCGATCCGATTCCCGACTTAATGTTGCCACAACAACCAGATATGGGCGCGGCGCAT
TCAATTGCACCTGCTTTACTCACGTATCTGTTTAGCGAAAAATAA

Upstream 100 bases:

>100_bases
GCTTCATGCCACTTATGCTCCGGTTTCTGATCGCGCCAGCTCGCGCTCTTGTTGTCTGCGTGTTAATCTAATTCTAGATC
ATCAAATAAAGGAATGACAC

Downstream 100 bases:

>100_bases
AACATCTGCCGTCATGTTCGGCAGATGTTGGTTATAACTTTTTAAAAAAATCAAAGCCACGTTTTTTCGGCTTTTCAGAA
TCTTGCTCAAGTGGTGGCAG

Product: glutathione biosynthesis bifunctional protein

Products: NA

Alternate protein names: Gamma-GCS-GS; GCS-GS; Glutamate--cysteine ligase; Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase; Glutathione synthetase; GSH synthetase; GS; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 654; Mature: 654

Protein sequence:

>654_residues
MLNHYWQLIQKNNLFGLRDDTRIGVIRTVKPVLAEDEQLPVWANASRHALVQQVSATMLQLTTPLLDDPSEVVAYLRAGL
SRVWAALPENGKLPVFTAGLENNQRIRIVLPEVLFERLYTAENFQGNGPDYVTYRNQIYDLVTDGLITQLPLLTYLFAAS
PAKNGRAFWPAPDQERQLVQKVSDPDHLARTLALDVAVELDPYAASGVTEQMLNFLIDSCWFALSQPAIPLPAAAEVRLH
SLAEAQKIAAGDPTAPVASLTAPIDAMAVWLNQVGLTPQRKQAFELMQSRVLKPETTIAGQVAAAYHDISAAQTPLATQA
HADLSMEEDLPGFSNLSGNSQALLQAVIDGGYQWTLLDRGQNILQIASDTQRHVLIDGALTSRTPASAMVVAEHRHAAKK
VLAAAGLPVARGAKFTRWPEAKAAFEQSFARKSIVVKPEQRSHGLAVEQFAVPPTAKQFAQAFHAANQDHGVLVEMMGRG
TTYHFTVIGRRVVSVLENAAANVVGDGRKSIKELIALKNGKRPNARQLKLDETANRQLKLQSVTMNTVLRRGQQIFLASA
AHPQTGGDIYDVTTEIDPSYNQLAVAAADALELPIAAVDIVIDNLYDAYAAEHEGQAIIISVDPIPDLMLPQQPDMGAAH
SIAPALLTYLFSEK

Sequences:

>Translated_654_residues
MLNHYWQLIQKNNLFGLRDDTRIGVIRTVKPVLAEDEQLPVWANASRHALVQQVSATMLQLTTPLLDDPSEVVAYLRAGL
SRVWAALPENGKLPVFTAGLENNQRIRIVLPEVLFERLYTAENFQGNGPDYVTYRNQIYDLVTDGLITQLPLLTYLFAAS
PAKNGRAFWPAPDQERQLVQKVSDPDHLARTLALDVAVELDPYAASGVTEQMLNFLIDSCWFALSQPAIPLPAAAEVRLH
SLAEAQKIAAGDPTAPVASLTAPIDAMAVWLNQVGLTPQRKQAFELMQSRVLKPETTIAGQVAAAYHDISAAQTPLATQA
HADLSMEEDLPGFSNLSGNSQALLQAVIDGGYQWTLLDRGQNILQIASDTQRHVLIDGALTSRTPASAMVVAEHRHAAKK
VLAAAGLPVARGAKFTRWPEAKAAFEQSFARKSIVVKPEQRSHGLAVEQFAVPPTAKQFAQAFHAANQDHGVLVEMMGRG
TTYHFTVIGRRVVSVLENAAANVVGDGRKSIKELIALKNGKRPNARQLKLDETANRQLKLQSVTMNTVLRRGQQIFLASA
AHPQTGGDIYDVTTEIDPSYNQLAVAAADALELPIAAVDIVIDNLYDAYAAEHEGQAIIISVDPIPDLMLPQQPDMGAAH
SIAPALLTYLFSEK
>Mature_654_residues
MLNHYWQLIQKNNLFGLRDDTRIGVIRTVKPVLAEDEQLPVWANASRHALVQQVSATMLQLTTPLLDDPSEVVAYLRAGL
SRVWAALPENGKLPVFTAGLENNQRIRIVLPEVLFERLYTAENFQGNGPDYVTYRNQIYDLVTDGLITQLPLLTYLFAAS
PAKNGRAFWPAPDQERQLVQKVSDPDHLARTLALDVAVELDPYAASGVTEQMLNFLIDSCWFALSQPAIPLPAAAEVRLH
SLAEAQKIAAGDPTAPVASLTAPIDAMAVWLNQVGLTPQRKQAFELMQSRVLKPETTIAGQVAAAYHDISAAQTPLATQA
HADLSMEEDLPGFSNLSGNSQALLQAVIDGGYQWTLLDRGQNILQIASDTQRHVLIDGALTSRTPASAMVVAEHRHAAKK
VLAAAGLPVARGAKFTRWPEAKAAFEQSFARKSIVVKPEQRSHGLAVEQFAVPPTAKQFAQAFHAANQDHGVLVEMMGRG
TTYHFTVIGRRVVSVLENAAANVVGDGRKSIKELIALKNGKRPNARQLKLDETANRQLKLQSVTMNTVLRRGQQIFLASA
AHPQTGGDIYDVTTEIDPSYNQLAVAAADALELPIAAVDIVIDNLYDAYAAEHEGQAIIISVDPIPDLMLPQQPDMGAAH
SIAPALLTYLFSEK

Specific function: Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the glutamate-- cysteine ligase type 1 family. Type 2 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2; =6.3.2.3 [H]

Molecular weight: Translated: 71225; Mature: 71225

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNHYWQLIQKNNLFGLRDDTRIGVIRTVKPVLAEDEQLPVWANASRHALVQQVSATMLQ
CCCHHHHHHHCCCEECCCCCCCEEHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHH
LTTPLLDDPSEVVAYLRAGLSRVWAALPENGKLPVFTAGLENNQRIRIVLPEVLFERLYT
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEEHHHHHHHHHH
AENFQGNGPDYVTYRNQIYDLVTDGLITQLPLLTYLFAASPAKNGRAFWPAPDQERQLVQ
HCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHH
KVSDPDHLARTLALDVAVELDPYAASGVTEQMLNFLIDSCWFALSQPAIPLPAAAEVRLH
HCCCHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
SLAEAQKIAAGDPTAPVASLTAPIDAMAVWLNQVGLTPQRKQAFELMQSRVLKPETTIAG
HHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHH
QVAAAYHDISAAQTPLATQAHADLSMEEDLPGFSNLSGNSQALLQAVIDGGYQWTLLDRG
HHHHHHHHHHHHCCCCCCHHCCCCCHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCC
QNILQIASDTQRHVLIDGALTSRTPASAMVVAEHRHAAKKVLAAAGLPVARGAKFTRWPE
CCEEEHHCCCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCHHCCCCCCCCCC
AKAAFEQSFARKSIVVKPEQRSHGLAVEQFAVPPTAKQFAQAFHAANQDHGVLVEMMGRG
HHHHHHHHHHHCCEEECCCCCCCCCEEHHHCCCCCHHHHHHHHHCCCCCCCEEEEEECCC
TTYHFTVIGRRVVSVLENAAANVVGDGRKSIKELIALKNGKRPNARQLKLDETANRQLKL
CEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEE
QSVTMNTVLRRGQQIFLASAAHPQTGGDIYDVTTEIDPSYNQLAVAAADALELPIAAVDI
EHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCEEEEHHHHHHCCHHHHHH
VIDNLYDAYAAEHEGQAIIISVDPIPDLMLPQQPDMGAAHSIAPALLTYLFSEK
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLNHYWQLIQKNNLFGLRDDTRIGVIRTVKPVLAEDEQLPVWANASRHALVQQVSATMLQ
CCCHHHHHHHCCCEECCCCCCCEEHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHH
LTTPLLDDPSEVVAYLRAGLSRVWAALPENGKLPVFTAGLENNQRIRIVLPEVLFERLYT
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEEHHHHHHHHHH
AENFQGNGPDYVTYRNQIYDLVTDGLITQLPLLTYLFAASPAKNGRAFWPAPDQERQLVQ
HCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHH
KVSDPDHLARTLALDVAVELDPYAASGVTEQMLNFLIDSCWFALSQPAIPLPAAAEVRLH
HCCCHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
SLAEAQKIAAGDPTAPVASLTAPIDAMAVWLNQVGLTPQRKQAFELMQSRVLKPETTIAG
HHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHH
QVAAAYHDISAAQTPLATQAHADLSMEEDLPGFSNLSGNSQALLQAVIDGGYQWTLLDRG
HHHHHHHHHHHHCCCCCCHHCCCCCHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCC
QNILQIASDTQRHVLIDGALTSRTPASAMVVAEHRHAAKKVLAAAGLPVARGAKFTRWPE
CCEEEHHCCCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCHHCCCCCCCCCC
AKAAFEQSFARKSIVVKPEQRSHGLAVEQFAVPPTAKQFAQAFHAANQDHGVLVEMMGRG
HHHHHHHHHHHCCEEECCCCCCCCCEEHHHCCCCCHHHHHHHHHCCCCCCCEEEEEECCC
TTYHFTVIGRRVVSVLENAAANVVGDGRKSIKELIALKNGKRPNARQLKLDETANRQLKL
CEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEE
QSVTMNTVLRRGQQIFLASAAHPQTGGDIYDVTTEIDPSYNQLAVAAADALELPIAAVDI
EHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCEEEEHHHHHHCCHHHHHH
VIDNLYDAYAAEHEGQAIIISVDPIPDLMLPQQPDMGAAHSIAPALLTYLFSEK
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]