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The map label for this gene is est [H]

Identifier: 116494481

GI number: 116494481

Start: 957748

End: 958482

Strand: Direct

Name: est [H]

Synonym: LSEI_0975

Alternate gene names: 116494481

Gene position: 957748-958482 (Clockwise)

Preceding gene: 116494480

Following gene: 116494482

Centisome position: 33.08

GC content: 49.66

Gene sequence:

>735_bases
ATGATCTTTCGTGAACCGCAACCATTCGAATACGAGGGCACTGATACCGGTGTTGTTTTGCTGCATGCCTATACTGGCAG
CCCAAACGACATGAACTTCATGGGGCGGGCCTTGCAGCGGGCTGGGTATGGCGTGTATGCGCCGTTGTTCACGGGACATG
GTACCATTGAGCCAATGGATATTCTGACTAAGGGCAACCCAGATATTTGGTGGGCAGAGGCCAGCGCGGCTGTTTCGCAT
ATGACAGCAAAGTATGACCGTGTTTTTGTTTTTGGATTATCACTCGGCGGTATTTTCGCCATGAAAGCTTTGGAAATATT
ACCCGGTGTCACAGCTGGCGGCGTTTTTTCATCGCCGATCTTACCGGGTAAGCACCATCTCGTGCCAGGGTTTCTGAAAT
ACGCACAATATATGAATCGTTTGGCAGGTGTACCAGACGAAAGTACTCAGATTCTGGCTTACTTACCGGGCCAGCTGGCG
GCGATTGACAATTTCGCCACTGGGGTTGCCGCCGATCTGTATTTAGTTAATAAGCCGATCTTTATCGGCCAGGCCGGTGC
TGATGAATTAGTTGATGGGCAGCTGGCTTATCAGCTACGGGATGCACTCATCAATGCACCGCAAGTAGATTTTCATTGGT
ATGACAATGCCAAGCATGTGATCACCGTGAATTCGGCGCATCACGCACTACAACAAGACGTTATCGCATTTATGCAACAA
GAAAACGAGGGATAG

Upstream 100 bases:

>100_bases
ACCTACAACCGTTTTTTCACCGCAATATCGAGCCTTTTCACCAAGAGATTGAATTCCCACTTGTCCACCCAGCCACTTGC
CACAAAGGATGAAACCCATC

Downstream 100 bases:

>100_bases
CATGACCACAGTTGGCCATATTCGTAATGAACTTTTAGCAACATTTCGTAAAAATCCGAAGATTGATTATTCGGTCCAGA
CGCTGAGCCGTGCCTTGAAA

Product: esterase/lipase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MIFREPQPFEYEGTDTGVVLLHAYTGSPNDMNFMGRALQRAGYGVYAPLFTGHGTIEPMDILTKGNPDIWWAEASAAVSH
MTAKYDRVFVFGLSLGGIFAMKALEILPGVTAGGVFSSPILPGKHHLVPGFLKYAQYMNRLAGVPDESTQILAYLPGQLA
AIDNFATGVAADLYLVNKPIFIGQAGADELVDGQLAYQLRDALINAPQVDFHWYDNAKHVITVNSAHHALQQDVIAFMQQ
ENEG

Sequences:

>Translated_244_residues
MIFREPQPFEYEGTDTGVVLLHAYTGSPNDMNFMGRALQRAGYGVYAPLFTGHGTIEPMDILTKGNPDIWWAEASAAVSH
MTAKYDRVFVFGLSLGGIFAMKALEILPGVTAGGVFSSPILPGKHHLVPGFLKYAQYMNRLAGVPDESTQILAYLPGQLA
AIDNFATGVAADLYLVNKPIFIGQAGADELVDGQLAYQLRDALINAPQVDFHWYDNAKHVITVNSAHHALQQDVIAFMQQ
ENEG
>Mature_244_residues
MIFREPQPFEYEGTDTGVVLLHAYTGSPNDMNFMGRALQRAGYGVYAPLFTGHGTIEPMDILTKGNPDIWWAEASAAVSH
MTAKYDRVFVFGLSLGGIFAMKALEILPGVTAGGVFSSPILPGKHHLVPGFLKYAQYMNRLAGVPDESTQILAYLPGQLA
AIDNFATGVAADLYLVNKPIFIGQAGADELVDGQLAYQLRDALINAPQVDFHWYDNAKHVITVNSAHHALQQDVIAFMQQ
ENEG

Specific function: Involved in the detoxification of xenobiotics. Shows maximal activity with C6 substrates, with gradually decreasing activity from C8 to C12 substrates. No activity for higher chain length substrates acids rather than long-chain ones [H]

COG id: COG1647

COG function: function code R; Esterase/lipase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipase/esterase LIP3/BchO family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012354
- InterPro:   IPR001375 [H]

Pfam domain/function: PF00326 Peptidase_S9 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 26584; Mature: 26584

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFREPQPFEYEGTDTGVVLLHAYTGSPNDMNFMGRALQRAGYGVYAPLFTGHGTIEPMD
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHH
ILTKGNPDIWWAEASAAVSHMTAKYDRVFVFGLSLGGIFAMKALEILPGVTAGGVFSSPI
EEECCCCCEEEEHHHHHHHHHHHHHCEEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCC
LPGKHHLVPGFLKYAQYMNRLAGVPDESTQILAYLPGQLAAIDNFATGVAADLYLVNKPI
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHEEEEEECCCE
FIGQAGADELVDGQLAYQLRDALINAPQVDFHWYDNAKHVITVNSAHHALQQDVIAFMQQ
EEECCCCCHHHCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHH
ENEG
CCCC
>Mature Secondary Structure
MIFREPQPFEYEGTDTGVVLLHAYTGSPNDMNFMGRALQRAGYGVYAPLFTGHGTIEPMD
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHH
ILTKGNPDIWWAEASAAVSHMTAKYDRVFVFGLSLGGIFAMKALEILPGVTAGGVFSSPI
EEECCCCCEEEEHHHHHHHHHHHHHCEEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCC
LPGKHHLVPGFLKYAQYMNRLAGVPDESTQILAYLPGQLAAIDNFATGVAADLYLVNKPI
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHEEEEEECCCE
FIGQAGADELVDGQLAYQLRDALINAPQVDFHWYDNAKHVITVNSAHHALQQDVIAFMQQ
EEECCCCCHHHCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHH
ENEG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1369099 [H]