Click here to switch to the map view.

The map label for this gene is metK

Identifier: 116494386

GI number: 116494386

Start: 852037

End: 853221

Strand: Direct

Name: metK

Synonym: LSEI_0877

Alternate gene names: 116494386

Gene position: 852037-853221 (Clockwise)

Preceding gene: 116494384

Following gene: 116494387

Centisome position: 29.43

GC content: 48.35

Gene sequence:

>1185_bases
ATGCAGGAACGCCATTTATTTACGTCGGAGTCTGTTTCGGAGGGGCATCCGGATAAAATTGCTGATCAGATCAGTGATGC
TATCTTGGATGCCATGCTTGAACAGGATCCCGATTCACGAGTTGCTTGCGAAACGACGGTGACAACCGGTTTGGTGCTGG
TTGTCGGTGAGATATCAACAAAGGCTTACGTTGATATTCAATCAGTGGTTCGCGGGACGATTAAAAAGATCGGCTATACC
AAGGAATCAGGTTTTGATCCTGATAGTGTCGGGGTGCTGGTCGCTTTGGATGAGCAGAGTCCAGATATCGCTCAAGGGGT
TGACGAAAGTCTTGAAGCCCGTGAGTCTGATACTGATCCGCTTGACAAGATCGGTGCTGGCGATCAAGGCATGATGTTCG
GTTTTGCCATTGACGAAACCGAAAACTATATGCCGTTACCGATTTCATTGGCCCACGCGTTAATGCGCAAAACTGATTCA
TTACGGCACAAGGGTGAAATTAGTTATCTGCGTCCAGATGCTAAGGCGCAGGTCACGGTGGAATATGATGATGATGACAA
TCCAATTCGGGTTGATTCCGTTGTTGTCTCCGTGCAACATGATCCGGATGTCACGCTTGAAGAAATTCGCCGCGATGTCG
AGGCCAAGATTATTCGCACTGTGATTCCTGAAGCTTTAATGGATGATGACACGAAAATTTATGTCAATCCGACTGGCCGC
TTTGTACTGGGCGGTCCGCAAGCCGACTCTGGTTTGACCGGACGCAAAATTATCGTTGACACATATGGCGGTTTTGCCCG
ACATGGTGGTGGTGCCTTTTCTGGTAAGGACGCGACGAAGGTTGATCGTTCGGCCAGTTATGCAGCGCGTTATATTGCCA
AAAATGTGGTAGCAGCGGGTTTGGCGAAACGGGTTGAAGTACAATTGGCATACGCAATCGGTGTTGCCAAACCGGTTTCC
GTGTCGGTGAATACATTTGGCACAAGCGCAGTATCAGAAGATGTGATTGAACAGGCAATCCGTGAGAACTTTGACTTGCG
GCCTGCTGGCATTATTAAAATGTTAGACCTGAAGCGGCCGATTTATGAACAGACGGCAGCATATGGTCATTTTGGACGTA
CAGATGTTGATCTTCCTTGGGAGCACTTGGATAAGGTGCAGGCTTTGCTCAAGTACCGAGATTAG

Upstream 100 bases:

>100_bases
TTATGTTATGCTACTAGAGTTGCAAAACATTCCCGATGGGGTTCGTCAAAACTATGACGAACATGCCTTGTAACCTAAAA
TAATAAGGGGGAAACAAAAC

Downstream 100 bases:

>100_bases
CGAGAAAAGAGGCGGCCTTAGTCGAGAATTATTCTCGGCTGGGTCGTTTTTTCGTGCTTATGAGGGGCTTCTCAAGACTA
ACAACATCGCCTGGCAACTC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MQERHLFTSESVSEGHPDKIADQISDAILDAMLEQDPDSRVACETTVTTGLVLVVGEISTKAYVDIQSVVRGTIKKIGYT
KESGFDPDSVGVLVALDEQSPDIAQGVDESLEARESDTDPLDKIGAGDQGMMFGFAIDETENYMPLPISLAHALMRKTDS
LRHKGEISYLRPDAKAQVTVEYDDDDNPIRVDSVVVSVQHDPDVTLEEIRRDVEAKIIRTVIPEALMDDDTKIYVNPTGR
FVLGGPQADSGLTGRKIIVDTYGGFARHGGGAFSGKDATKVDRSASYAARYIAKNVVAAGLAKRVEVQLAYAIGVAKPVS
VSVNTFGTSAVSEDVIEQAIRENFDLRPAGIIKMLDLKRPIYEQTAAYGHFGRTDVDLPWEHLDKVQALLKYRD

Sequences:

>Translated_394_residues
MQERHLFTSESVSEGHPDKIADQISDAILDAMLEQDPDSRVACETTVTTGLVLVVGEISTKAYVDIQSVVRGTIKKIGYT
KESGFDPDSVGVLVALDEQSPDIAQGVDESLEARESDTDPLDKIGAGDQGMMFGFAIDETENYMPLPISLAHALMRKTDS
LRHKGEISYLRPDAKAQVTVEYDDDDNPIRVDSVVVSVQHDPDVTLEEIRRDVEAKIIRTVIPEALMDDDTKIYVNPTGR
FVLGGPQADSGLTGRKIIVDTYGGFARHGGGAFSGKDATKVDRSASYAARYIAKNVVAAGLAKRVEVQLAYAIGVAKPVS
VSVNTFGTSAVSEDVIEQAIRENFDLRPAGIIKMLDLKRPIYEQTAAYGHFGRTDVDLPWEHLDKVQALLKYRD
>Mature_394_residues
MQERHLFTSESVSEGHPDKIADQISDAILDAMLEQDPDSRVACETTVTTGLVLVVGEISTKAYVDIQSVVRGTIKKIGYT
KESGFDPDSVGVLVALDEQSPDIAQGVDESLEARESDTDPLDKIGAGDQGMMFGFAIDETENYMPLPISLAHALMRKTDS
LRHKGEISYLRPDAKAQVTVEYDDDDNPIRVDSVVVSVQHDPDVTLEEIRRDVEAKIIRTVIPEALMDDDTKIYVNPTGR
FVLGGPQADSGLTGRKIIVDTYGGFARHGGGAFSGKDATKVDRSASYAARYIAKNVVAAGLAKRVEVQLAYAIGVAKPVS
VSVNTFGTSAVSEDVIEQAIRENFDLRPAGIIKMLDLKRPIYEQTAAYGHFGRTDVDLPWEHLDKVQALLKYRD

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=57.8947368421053, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI5174529, Length=391, Percent_Identity=58.0562659846547, Blast_Score=429, Evalue=1e-120,
Organism=Escherichia coli, GI1789311, Length=387, Percent_Identity=58.3979328165375, Blast_Score=464, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17538498, Length=385, Percent_Identity=57.9220779220779, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI25145633, Length=390, Percent_Identity=57.1794871794872, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17538494, Length=390, Percent_Identity=56.6666666666667, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17551082, Length=385, Percent_Identity=56.1038961038961, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32565905, Length=389, Percent_Identity=51.413881748072, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565911, Length=336, Percent_Identity=55.952380952381, Blast_Score=358, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI25145635, Length=341, Percent_Identity=55.425219941349, Blast_Score=356, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI32565903, Length=308, Percent_Identity=54.8701298701299, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI32565909, Length=121, Percent_Identity=47.9338842975207, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI32565907, Length=42, Percent_Identity=73.8095238095238, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=385, Percent_Identity=57.4025974025974, Blast_Score=421, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6320710, Length=386, Percent_Identity=55.9585492227979, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI45552159, Length=380, Percent_Identity=55, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580529, Length=380, Percent_Identity=55, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580537, Length=380, Percent_Identity=55, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580533, Length=380, Percent_Identity=55, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580535, Length=380, Percent_Identity=55, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580531, Length=380, Percent_Identity=55, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580527, Length=380, Percent_Identity=54.2105263157895, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580525, Length=380, Percent_Identity=54.2105263157895, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580541, Length=382, Percent_Identity=51.8324607329843, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580539, Length=125, Percent_Identity=56, Blast_Score=138, Evalue=5e-33,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_LACC3 (Q03AU4)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806120.1
- ProteinModelPortal:   Q03AU4
- SMR:   Q03AU4
- STRING:   Q03AU4
- GeneID:   4421692
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_0877
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   QDEHARV
- PhylomeDB:   Q03AU4
- ProtClustDB:   PRK05250
- BioCyc:   LCAS321967:LSEI_0877-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 43001; Mature: 43001

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQERHLFTSESVSEGHPDKIADQISDAILDAMLEQDPDSRVACETTVTTGLVLVVGEIST
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCEEEEEECCCC
KAYVDIQSVVRGTIKKIGYTKESGFDPDSVGVLVALDEQSPDIAQGVDESLEARESDTDP
HHHEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCC
LDKIGAGDQGMMFGFAIDETENYMPLPISLAHALMRKTDSLRHKGEISYLRPDAKAQVTV
HHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEE
EYDDDDNPIRVDSVVVSVQHDPDVTLEEIRRDVEAKIIRTVIPEALMDDDTKIYVNPTGR
EECCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
FVLGGPQADSGLTGRKIIVDTYGGFARHGGGAFSGKDATKVDRSASYAARYIAKNVVAAG
EEECCCCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LAKRVEVQLAYAIGVAKPVSVSVNTFGTSAVSEDVIEQAIRENFDLRPAGIIKMLDLKRP
HHHHHHEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCH
IYEQTAAYGHFGRTDVDLPWEHLDKVQALLKYRD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQERHLFTSESVSEGHPDKIADQISDAILDAMLEQDPDSRVACETTVTTGLVLVVGEIST
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCEEEEEECCCC
KAYVDIQSVVRGTIKKIGYTKESGFDPDSVGVLVALDEQSPDIAQGVDESLEARESDTDP
HHHEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCC
LDKIGAGDQGMMFGFAIDETENYMPLPISLAHALMRKTDSLRHKGEISYLRPDAKAQVTV
HHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEE
EYDDDDNPIRVDSVVVSVQHDPDVTLEEIRRDVEAKIIRTVIPEALMDDDTKIYVNPTGR
EECCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
FVLGGPQADSGLTGRKIIVDTYGGFARHGGGAFSGKDATKVDRSASYAARYIAKNVVAAG
EEECCCCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LAKRVEVQLAYAIGVAKPVSVSVNTFGTSAVSEDVIEQAIRENFDLRPAGIIKMLDLKRP
HHHHHHEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCH
IYEQTAAYGHFGRTDVDLPWEHLDKVQALLKYRD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA