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The map label for this gene is 116493976

Identifier: 116493976

GI number: 116493976

Start: 418835

End: 419821

Strand: Direct

Name: 116493976

Synonym: LSEI_0412

Alternate gene names: NA

Gene position: 418835-419821 (Clockwise)

Preceding gene: 116493975

Following gene: 116493978

Centisome position: 14.47

GC content: 48.33

Gene sequence:

>987_bases
ATGTGTTCTTCAATGACAATTAAATCGCTACAAGGTGATATTTTCTGGGGCCGGACCATGGATTACAACACCAGTTTCTT
TCATGAATCACCAGTCGGCGGGGTTCCTGGTAAAATTGTCAGTTTGCCAGCAAACAAGGCATTACCAACACAAAGTGCCA
AATGGACCACAAAGTATGCAGCGGTTGGCGTCGGTATTGATCAAAGTACGGCACTTTTCGACGGCGTCAATAGCGAAGGC
TTGGCTGGCGACTTGCAGGTTTTGGTAGAGTGCAGCTGGGCGAGTGCAGATTCTTTGAAACAGCGTGGGCTGAAAGCTAT
TAAAGGCGAGGAGTTTGTGACCTTGGCCTTGACGACATGCAAAAATGTTGACGAAGTACGCGCTTTGGCGGGTGAGTATG
GCCTGCTGGATGAACCTTATGAGTTTGGCGGCCAAGGGGTGAAAATCCCATTACATTACACATTTGTTGATCCATCAGGT
AAAGGGATTGTTGTCGAACCAACCGATCACGGCGCCTTTAAGCTGTATGATAGTATCGGCGCCATGACGAATAGTCCTGA
ATACGGGTGGCATGAAACCAATTTGCGCAATTATGTCAGTCTGAATGACAATAACTATCCTAAGGGGTCCGAATTAGGCG
ACTACCATATTGAGCCGATTGAGTTAGGCACAGGCTACGGGATGTTCGGTTTGCCAGGCGATTACACATCACCATCGCGG
TTCCTGCGGGCAATGTTTGTTTCGCGTAATCTTGATCCCTTCAACAGCAAAGACGGCATTCGCGTGTTATACAATGCTTT
CAAGACTGTCTTGATTCCGCAGGGGCTAGGTCGCGACCCACAACATCAGGTTTTAACGGATTACACGCAATACTGGTCTG
GCTATGACCTGACGAAAAAGACGATTTGGGCGTGCGGACGTTTTTCTGGACTTTCACCATCAAGCTACCTTGCTCTGACG
GTATTCCTGCGGGCTCTTTCCTCCTAA

Upstream 100 bases:

>100_bases
TGAAGGGGATCGAGCCATTAAGCATTTCGGGTTATAATGACAGTAATCGTAAAAATTAAGCTTTAAACACTGACATTTAT
TTTCGAAAGGGGTCGTTGCT

Downstream 100 bases:

>100_bases
AACTTTTTTTCTTCGTTTGGTGTTATACCACTCAATATACTCATTGAGTGCTTGTGTGAAATCATCAATTGACTTTCCAA
GCCAGCTTCGATTGTAAAAG

Product: conjugated bile salt hydrolase-like protein

Products: NA

Alternate protein names: Penicillin V amidase; PVA [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MCSSMTIKSLQGDIFWGRTMDYNTSFFHESPVGGVPGKIVSLPANKALPTQSAKWTTKYAAVGVGIDQSTALFDGVNSEG
LAGDLQVLVECSWASADSLKQRGLKAIKGEEFVTLALTTCKNVDEVRALAGEYGLLDEPYEFGGQGVKIPLHYTFVDPSG
KGIVVEPTDHGAFKLYDSIGAMTNSPEYGWHETNLRNYVSLNDNNYPKGSELGDYHIEPIELGTGYGMFGLPGDYTSPSR
FLRAMFVSRNLDPFNSKDGIRVLYNAFKTVLIPQGLGRDPQHQVLTDYTQYWSGYDLTKKTIWACGRFSGLSPSSYLALT
VFLRALSS

Sequences:

>Translated_328_residues
MCSSMTIKSLQGDIFWGRTMDYNTSFFHESPVGGVPGKIVSLPANKALPTQSAKWTTKYAAVGVGIDQSTALFDGVNSEG
LAGDLQVLVECSWASADSLKQRGLKAIKGEEFVTLALTTCKNVDEVRALAGEYGLLDEPYEFGGQGVKIPLHYTFVDPSG
KGIVVEPTDHGAFKLYDSIGAMTNSPEYGWHETNLRNYVSLNDNNYPKGSELGDYHIEPIELGTGYGMFGLPGDYTSPSR
FLRAMFVSRNLDPFNSKDGIRVLYNAFKTVLIPQGLGRDPQHQVLTDYTQYWSGYDLTKKTIWACGRFSGLSPSSYLALT
VFLRALSS
>Mature_328_residues
MCSSMTIKSLQGDIFWGRTMDYNTSFFHESPVGGVPGKIVSLPANKALPTQSAKWTTKYAAVGVGIDQSTALFDGVNSEG
LAGDLQVLVECSWASADSLKQRGLKAIKGEEFVTLALTTCKNVDEVRALAGEYGLLDEPYEFGGQGVKIPLHYTFVDPSG
KGIVVEPTDHGAFKLYDSIGAMTNSPEYGWHETNLRNYVSLNDNNYPKGSELGDYHIEPIELGTGYGMFGLPGDYTSPSR
FLRAMFVSRNLDPFNSKDGIRVLYNAFKTVLIPQGLGRDPQHQVLTDYTQYWSGYDLTKKTIWACGRFSGLSPSSYLALT
VFLRALSS

Specific function: The enzyme catalyzes the conversion of penicillin to 6- aminopenicillanate The precursor, furthermore, acts as a self- processing peptidase that cleaves off the propeptide. All peptidase activity is lost on conversion to the mature peptidase [H]

COG id: COG3049

COG function: function code M; Penicillin V acylase and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C59 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003199 [H]

Pfam domain/function: PF02275 CBAH [H]

EC number: =3.5.1.11 [H]

Molecular weight: Translated: 35944; Mature: 35944

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSSMTIKSLQGDIFWGRTMDYNTSFFHESPVGGVPGKIVSLPANKALPTQSAKWTTKYA
CCCCCCHHHCCCCEEECCEECCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEEE
AVGVGIDQSTALFDGVNSEGLAGDLQVLVECSWASADSLKQRGLKAIKGEEFVTLALTTC
EEECCCCCCHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHCHHHCCCCCEEEEEEEHH
KNVDEVRALAGEYGLLDEPYEFGGQGVKIPLHYTFVDPSGKGIVVEPTDHGAFKLYDSIG
CCHHHHHHHHHCCCCCCCCHHHCCCEEEEEEEEEEECCCCCEEEEECCCCCCEEEHHHHC
AMTNSPEYGWHETNLRNYVSLNDNNYPKGSELGDYHIEPIELGTGYGMFGLPGDYTSPSR
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEECCEEEECCCCCCCCCCCCCCCHHH
FLRAMFVSRNLDPFNSKDGIRVLYNAFKTVLIPQGLGRDPQHQVLTDYTQYWSGYDLTKK
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCCCCHH
TIWACGRFSGLSPSSYLALTVFLRALSS
HHHHHHCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCSSMTIKSLQGDIFWGRTMDYNTSFFHESPVGGVPGKIVSLPANKALPTQSAKWTTKYA
CCCCCCHHHCCCCEEECCEECCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEEE
AVGVGIDQSTALFDGVNSEGLAGDLQVLVECSWASADSLKQRGLKAIKGEEFVTLALTTC
EEECCCCCCHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHCHHHCCCCCEEEEEEEHH
KNVDEVRALAGEYGLLDEPYEFGGQGVKIPLHYTFVDPSGKGIVVEPTDHGAFKLYDSIG
CCHHHHHHHHHCCCCCCCCHHHCCCEEEEEEEEEEECCCCCEEEEECCCCCCEEEHHHHC
AMTNSPEYGWHETNLRNYVSLNDNNYPKGSELGDYHIEPIELGTGYGMFGLPGDYTSPSR
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEECCEEEECCCCCCCCCCCCCCCHHH
FLRAMFVSRNLDPFNSKDGIRVLYNAFKTVLIPQGLGRDPQHQVLTDYTQYWSGYDLTKK
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHCCCCCCHH
TIWACGRFSGLSPSSYLALTVFLRALSS
HHHHHHCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3026906 [H]