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The map label for this gene is php [H]

Identifier: 116493946

GI number: 116493946

Start: 379637

End: 380521

Strand: Direct

Name: php [H]

Synonym: LSEI_0380

Alternate gene names: 116493946

Gene position: 379637-380521 (Clockwise)

Preceding gene: 116493945

Following gene: 116493947

Centisome position: 13.11

GC content: 40.45

Gene sequence:

>885_bases
ATGTTATTACCAGGTAAGGTTTATGCTCATGAACACATTCCAATTGATTTATCTGAAGTCAAGCAAAACGAGGATTGTCA
TCTTGATGCCATTGATCAAGTGATTGCAGAATTTAAGGATCTTTATCAAAAAGGTGTTCGTAATGTTATTAGCATGACGA
ACAAAGGTATGGGTCGAAATATTCCATATGCTAATAAAGTAGCAAAAGAATCTGGCATCAACATTGTGCAATGCACTGGG
TTCTATCAAGACGCCTTTTTACCAATTGAAGTCTTTCGGTTGTCTGTTAGTCAGTTAGCTGAACAGATGATTAAAGATAT
TCAAGTCGGTATTAAAGGAACAGATATTAAGGCTGGTGTAATTGGCGAAATTGCAACATCGAAAGGTCAGTGGACGGCGG
CTGAAGAAAAAGTTTTCCAGGCAGCGGTCATTGCTCAGCAGGAAACTGGGTGTCCGATTAGTACCCATACCTCAATTGGA
ACGTTGGGTCATGAACAGGTGGATTTCTTTAAACGGCAGCATGCCGATCTTAAACACATTGTTATCGGACATGTTGATCT
GACAGGCAGTGCCGACTACGTCTTAGACATGTTGAAAACTGGTATTAATGTGGAGTTTGACACAATTGGCAAAAATAATT
ATATGCCTGATGCAACGCGCGTTGAGATGTTAAAAGCGATTGAAGCCGCCGGTTTTACGGATCAAGTCGTTTTGTCCATG
GACATTACGCGTAAGTCACATCTGAAGGCTAATGGCGGGAATGGTTATGCTTATTTACTTGATTCATTTATTCCTCAGCT
GCAGGATGCGGGCATTTCTGAGACTTTCATCAACAAAATGATAGAAGCAAATCCTCAAAGAATCTACGGTGATATCAATG
CCTAG

Upstream 100 bases:

>100_bases
AGTGGGACCAATTGCTGCTATCGCGCTTGGAATTTTGCTGAATCTGTTACGAATCGTACATCTGTTTTAGTCGATGGCGA
GCGCATTAAGGGGAGAAAAC

Downstream 100 bases:

>100_bases
TTTTAAAACTTATCCATTAGAAAGCATCGGATATGACGAAGCGCTGCGGAAGCAATTTCGTTTAGTTGATGAGATTACTC
GCCATTTTAAGGGGTCTGAG

Product: metal-dependent hydrolase with the TIM-barrel fold

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MLLPGKVYAHEHIPIDLSEVKQNEDCHLDAIDQVIAEFKDLYQKGVRNVISMTNKGMGRNIPYANKVAKESGINIVQCTG
FYQDAFLPIEVFRLSVSQLAEQMIKDIQVGIKGTDIKAGVIGEIATSKGQWTAAEEKVFQAAVIAQQETGCPISTHTSIG
TLGHEQVDFFKRQHADLKHIVIGHVDLTGSADYVLDMLKTGINVEFDTIGKNNYMPDATRVEMLKAIEAAGFTDQVVLSM
DITRKSHLKANGGNGYAYLLDSFIPQLQDAGISETFINKMIEANPQRIYGDINA

Sequences:

>Translated_294_residues
MLLPGKVYAHEHIPIDLSEVKQNEDCHLDAIDQVIAEFKDLYQKGVRNVISMTNKGMGRNIPYANKVAKESGINIVQCTG
FYQDAFLPIEVFRLSVSQLAEQMIKDIQVGIKGTDIKAGVIGEIATSKGQWTAAEEKVFQAAVIAQQETGCPISTHTSIG
TLGHEQVDFFKRQHADLKHIVIGHVDLTGSADYVLDMLKTGINVEFDTIGKNNYMPDATRVEMLKAIEAAGFTDQVVLSM
DITRKSHLKANGGNGYAYLLDSFIPQLQDAGISETFINKMIEANPQRIYGDINA
>Mature_294_residues
MLLPGKVYAHEHIPIDLSEVKQNEDCHLDAIDQVIAEFKDLYQKGVRNVISMTNKGMGRNIPYANKVAKESGINIVQCTG
FYQDAFLPIEVFRLSVSQLAEQMIKDIQVGIKGTDIKAGVIGEIATSKGQWTAAEEKVFQAAVIAQQETGCPISTHTSIG
TLGHEQVDFFKRQHADLKHIVIGHVDLTGSADYVLDMLKTGINVEFDTIGKNNYMPDATRVEMLKAIEAAGFTDQVVLSM
DITRKSHLKANGGNGYAYLLDSFIPQLQDAGISETFINKMIEANPQRIYGDINA

Specific function: Its real enzymatic activity is not yet known. It was tested for general esterase, aminopeptidase, sulfatase, phosphatase, carbonic anhydrase, phosphodiesterase, and phosphotriesterase activities with the following substrates:P- nitrophenyl acetate, L-alan

COG id: COG1735

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphotriesterase family [H]

Homologues:

Organism=Homo sapiens, GI20070186, Length=273, Percent_Identity=30.03663003663, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI47933341, Length=273, Percent_Identity=30.03663003663, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1789780, Length=285, Percent_Identity=50.8771929824561, Blast_Score=303, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24645325, Length=262, Percent_Identity=25.9541984732824, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017947
- InterPro:   IPR001559 [H]

Pfam domain/function: PF02126 PTE [H]

EC number: NA

Molecular weight: Translated: 32373; Mature: 32373

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLPGKVYAHEHIPIDLSEVKQNEDCHLDAIDQVIAEFKDLYQKGVRNVISMTNKGMGRN
CCCCCCEEECCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IPYANKVAKESGINIVQCTGFYQDAFLPIEVFRLSVSQLAEQMIKDIQVGIKGTDIKAGV
CCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
IGEIATSKGQWTAAEEKVFQAAVIAQQETGCPISTHTSIGTLGHEQVDFFKRQHADLKHI
HHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEE
VIGHVDLTGSADYVLDMLKTGINVEFDTIGKNNYMPDATRVEMLKAIEAAGFTDQVVLSM
EEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
DITRKSHLKANGGNGYAYLLDSFIPQLQDAGISETFINKMIEANPQRIYGDINA
ECCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHEEECCCC
>Mature Secondary Structure
MLLPGKVYAHEHIPIDLSEVKQNEDCHLDAIDQVIAEFKDLYQKGVRNVISMTNKGMGRN
CCCCCCEEECCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IPYANKVAKESGINIVQCTGFYQDAFLPIEVFRLSVSQLAEQMIKDIQVGIKGTDIKAGV
CCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
IGEIATSKGQWTAAEEKVFQAAVIAQQETGCPISTHTSIGTLGHEQVDFFKRQHADLKHI
HHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEE
VIGHVDLTGSADYVLDMLKTGINVEFDTIGKNNYMPDATRVEMLKAIEAAGFTDQVVLSM
EEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEE
DITRKSHLKANGGNGYAYLLDSFIPQLQDAGISETFINKMIEANPQRIYGDINA
ECCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9548740 [H]