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The map label for this gene is purR [H]

Identifier: 116493874

GI number: 116493874

Start: 306385

End: 307392

Strand: Direct

Name: purR [H]

Synonym: LSEI_0306

Alternate gene names: 116493874

Gene position: 306385-307392 (Clockwise)

Preceding gene: 116493873

Following gene: 116493875

Centisome position: 10.58

GC content: 47.92

Gene sequence:

>1008_bases
ATGAAAAAGAAGATTTCGATTACTGATGTGGCAAAGGAAACTGGTTTGTCAGTCACAACCGTTTCACAAATCTTAAATGG
TAAAGGGGAACGGTTTAGCGAAAAATCGCGAAAACGGGTGCTTGCTGCCAAGAAGGCGTTGGGTTATGAACCGGATTTCT
TTGCCCGTGGGCTGGTTGGTAAGCGTGGCAACTCAATTGGTGTTGTGATACCTGATATCATGAATCCCTTCTTCGCGAGT
TTTGTGGTCAGTGTCGAAAAGGCAGCCATTCCACAGGGACTCTTTCCACAAATCTTTTCAATTAATGGTTTCCATGAGAA
TGTTGATTATTTTATTCGTCAGTTTTTAGGCGGTACTCAGCGTGGTTTGATTTTGGCGGCGCCAGAAGCGTCACAGGAGA
TCGTTCAAAAAGTGGAAGATCAGCTCCATCTGCCAATGGTCTTTATGGATCAGGCTACCACGCTGACATCTGGCGACAGC
ATTCGTATTGATGATTTTCATGCAGGTTTTCTTGTCGCTGACCATCTCATTAAACGTGGTCACCGCCGGATTGCGCTAGT
ATTGCCAGAGCCGCTGACCTTTAATCTGCGGGATCGTCTCGCTGGTTATCAACATGCGTTCGATTCACATAATGTTGCAC
TTGATCCGCAGTTAATCTTCCGCGCAACATTTGATCCGGAAGGCGGGCGCCAGGCTGTCGACCAGATAGTCAAAACTGAT
GCTACTGCTATTATTGCGATCAATGATGATGTCGCAAGCGGTGTTTACCGCGGGTTGTATGAGCATCATAAGCGGATTCC
AGAGGACTACGCGGTAGTTGGCTTCGATGATGTCTCACAGGCGCGATTCATGACCCCCGGGTTAACCACGATTGCGCAAC
CAATTGACGAACTTGGCCAACGCGCGATCGACATGTTGCTGGCCCAGATGGCTGGTGACCATCAAGATCGCAACGTGATT
GAACTGCCAGTGAAGTTGATTGAGCGCGGATCGACAAAACGGATTTAA

Upstream 100 bases:

>100_bases
GAATCTTGGCAGTTGACAGCGCTTCCAAAATCAGGTTAAGCTGATCTTGCAAGATTAAGTAAAACGTTTTACCTAGCAAA
TGATTTAGGTCGTGGACAGC

Downstream 100 bases:

>100_bases
AGCATAACGTGCATCTTAAAAAATAGGGTGCAGATGCCGAATAAGAGGAGGCGTTTTGATTGAAAAAAGGAACGGTTATT
AACACGCAACTGTCACAAGT

Product: ribose operon repressor

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKKKISITDVAKETGLSVTTVSQILNGKGERFSEKSRKRVLAAKKALGYEPDFFARGLVGKRGNSIGVVIPDIMNPFFAS
FVVSVEKAAIPQGLFPQIFSINGFHENVDYFIRQFLGGTQRGLILAAPEASQEIVQKVEDQLHLPMVFMDQATTLTSGDS
IRIDDFHAGFLVADHLIKRGHRRIALVLPEPLTFNLRDRLAGYQHAFDSHNVALDPQLIFRATFDPEGGRQAVDQIVKTD
ATAIIAINDDVASGVYRGLYEHHKRIPEDYAVVGFDDVSQARFMTPGLTTIAQPIDELGQRAIDMLLAQMAGDHQDRNVI
ELPVKLIERGSTKRI

Sequences:

>Translated_335_residues
MKKKISITDVAKETGLSVTTVSQILNGKGERFSEKSRKRVLAAKKALGYEPDFFARGLVGKRGNSIGVVIPDIMNPFFAS
FVVSVEKAAIPQGLFPQIFSINGFHENVDYFIRQFLGGTQRGLILAAPEASQEIVQKVEDQLHLPMVFMDQATTLTSGDS
IRIDDFHAGFLVADHLIKRGHRRIALVLPEPLTFNLRDRLAGYQHAFDSHNVALDPQLIFRATFDPEGGRQAVDQIVKTD
ATAIIAINDDVASGVYRGLYEHHKRIPEDYAVVGFDDVSQARFMTPGLTTIAQPIDELGQRAIDMLLAQMAGDHQDRNVI
ELPVKLIERGSTKRI
>Mature_335_residues
MKKKISITDVAKETGLSVTTVSQILNGKGERFSEKSRKRVLAAKKALGYEPDFFARGLVGKRGNSIGVVIPDIMNPFFAS
FVVSVEKAAIPQGLFPQIFSINGFHENVDYFIRQFLGGTQRGLILAAPEASQEIVQKVEDQLHLPMVFMDQATTLTSGDS
IRIDDFHAGFLVADHLIKRGHRRIALVLPEPLTFNLRDRLAGYQHAFDSHNVALDPQLIFRATFDPEGGRQAVDQIVKTD
ATAIIAINDDVASGVYRGLYEHHKRIPEDYAVVGFDDVSQARFMTPGLTTIAQPIDELGQRAIDMLLAQMAGDHQDRNVI
ELPVKLIERGSTKRI

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=330, Percent_Identity=33.030303030303, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1790194, Length=332, Percent_Identity=33.1325301204819, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=29.940119760479, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1789202, Length=298, Percent_Identity=31.2080536912752, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1789068, Length=313, Percent_Identity=27.7955271565495, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787906, Length=339, Percent_Identity=25.9587020648968, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1788474, Length=318, Percent_Identity=27.6729559748428, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=29.5522388059701, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787580, Length=323, Percent_Identity=25.6965944272446, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI48994940, Length=338, Percent_Identity=24.8520710059172, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790715, Length=335, Percent_Identity=22.9850746268657, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786268, Length=315, Percent_Identity=22.5396825396825, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37120; Mature: 37120

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKISITDVAKETGLSVTTVSQILNGKGERFSEKSRKRVLAAKKALGYEPDFFARGLVG
CCCCCCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
KRGNSIGVVIPDIMNPFFASFVVSVEKAAIPQGLFPQIFSINGFHENVDYFIRQFLGGTQ
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHEECCCCHHHHHHHHHHHHCCCC
RGLILAAPEASQEIVQKVEDQLHLPMVFMDQATTLTSGDSIRIDDFHAGFLVADHLIKRG
CCEEEECCCHHHHHHHHHHHHHCCCEEEECCCCEECCCCEEEEECCCCHHHHHHHHHHCC
HRRIALVLPEPLTFNLRDRLAGYQHAFDSHNVALDPQLIFRATFDPEGGRQAVDQIVKTD
CCEEEEEECCCCEECHHHHHHHHHHHCCCCCEEECCEEEEEEECCCCCCHHHHHHHHHCC
ATAIIAINDDVASGVYRGLYEHHKRIPEDYAVVGFDDVSQARFMTPGLTTIAQPIDELGQ
CEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCCHHHHHHHHHHHHH
RAIDMLLAQMAGDHQDRNVIELPVKLIERGSTKRI
HHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKKISITDVAKETGLSVTTVSQILNGKGERFSEKSRKRVLAAKKALGYEPDFFARGLVG
CCCCCCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
KRGNSIGVVIPDIMNPFFASFVVSVEKAAIPQGLFPQIFSINGFHENVDYFIRQFLGGTQ
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHEECCCCHHHHHHHHHHHHCCCC
RGLILAAPEASQEIVQKVEDQLHLPMVFMDQATTLTSGDSIRIDDFHAGFLVADHLIKRG
CCEEEECCCHHHHHHHHHHHHHCCCEEEECCCCEECCCCEEEEECCCCHHHHHHHHHHCC
HRRIALVLPEPLTFNLRDRLAGYQHAFDSHNVALDPQLIFRATFDPEGGRQAVDQIVKTD
CCEEEEEECCCCEECHHHHHHHHHHHCCCCCEEECCEEEEEEECCCCCCHHHHHHHHHCC
ATAIIAINDDVASGVYRGLYEHHKRIPEDYAVVGFDDVSQARFMTPGLTTIAQPIDELGQ
CEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCCCHHHHHHHHHHHHH
RAIDMLLAQMAGDHQDRNVIELPVKLIERGSTKRI
HHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]