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The map label for this gene is tenA [H]

Identifier: 116493865

GI number: 116493865

Start: 300135

End: 300827

Strand: Direct

Name: tenA [H]

Synonym: LSEI_0297

Alternate gene names: 116493865

Gene position: 300135-300827 (Clockwise)

Preceding gene: 116493864

Following gene: 116493866

Centisome position: 10.37

GC content: 49.93

Gene sequence:

>693_bases
ATGAAATTTTCTGAAAAAGCTCGGCAGCAAGTTGTTCCGTACTGGGAAGGCAGCTTCACTCACCCATTTATTACGGAGCT
ACAAGCTGGCCCCCTTGATCCGGCGATTTTCCGCTTTTATCTGATTCAGGATGCTTATTATCTGCAACATTTTACACATT
TATATACGCTGATTGCCGCACAGACGCAGGAGCCGGAATTGAAAGCTTATGCGCTGCAGTCGGCTGAAGGTTTGGCCCAA
GGTGAAATTGCGATACGCCACGATTTTTTCGCCGAATTGAACATCTCAACAACTGAGCTGGCGGCCACCACGATTGCGCC
GACCACGTATCACTATGTTTCCCACATGTACCGACAATTGGTTGAAGGCACGCCGCATGTGGCGATTGCGGGGATGCTGC
CATGTCCATGGTTGTATCAGGAGGTGGCACAGCAGTTGAAGCAACGAAAATCACCCGTGCCGATTTATCAGCGCTGGATT
GACATTTATAGCGGTGCGGATAACGCGGACGATCAGCGAGCGGCGATTGCGTTGATTGATCGGTTGTACGACCAAAGAAT
GCCGACTGAGCAGCAACAAATGCTGGCTGCGTTTCGGATCAGCAGTCAAATGGAATACAGCTTTTGGGAGATGGCGTATC
ACTTAGAAGCGTGGCCGAAAGGAGCAAAGGTTGATGACACAAAGCAGCGTTAA

Upstream 100 bases:

>100_bases
TATTGGCCATTAACGTCATAAAAAACTTGATTCTAGTAAAAATACTGTTTTTAGGCTTTGAATATGGTCAAGATAGACAG
AATTAAAGGAGCAAACGATG

Downstream 100 bases:

>100_bases
GAAACTGGCGATATTAGCGATGATGATTGCCTTAGATGTGGTGCTTTCACCCTTATTTCGCGTTGAAGGGATGGCACCTA
TGTCCAGCGTGGTCAATGTC

Product: putative transcription activator

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MKFSEKARQQVVPYWEGSFTHPFITELQAGPLDPAIFRFYLIQDAYYLQHFTHLYTLIAAQTQEPELKAYALQSAEGLAQ
GEIAIRHDFFAELNISTTELAATTIAPTTYHYVSHMYRQLVEGTPHVAIAGMLPCPWLYQEVAQQLKQRKSPVPIYQRWI
DIYSGADNADDQRAAIALIDRLYDQRMPTEQQQMLAAFRISSQMEYSFWEMAYHLEAWPKGAKVDDTKQR

Sequences:

>Translated_230_residues
MKFSEKARQQVVPYWEGSFTHPFITELQAGPLDPAIFRFYLIQDAYYLQHFTHLYTLIAAQTQEPELKAYALQSAEGLAQ
GEIAIRHDFFAELNISTTELAATTIAPTTYHYVSHMYRQLVEGTPHVAIAGMLPCPWLYQEVAQQLKQRKSPVPIYQRWI
DIYSGADNADDQRAAIALIDRLYDQRMPTEQQQMLAAFRISSQMEYSFWEMAYHLEAWPKGAKVDDTKQR
>Mature_230_residues
MKFSEKARQQVVPYWEGSFTHPFITELQAGPLDPAIFRFYLIQDAYYLQHFTHLYTLIAAQTQEPELKAYALQSAEGLAQ
GEIAIRHDFFAELNISTTELAATTIAPTTYHYVSHMYRQLVEGTPHVAIAGMLPCPWLYQEVAQQLKQRKSPVPIYQRWI
DIYSGADNADDQRAAIALIDRLYDQRMPTEQQQMLAAFRISSQMEYSFWEMAYHLEAWPKGAKVDDTKQR

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 26616; Mature: 26616

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSEKARQQVVPYWEGSFTHPFITELQAGPLDPAIFRFYLIQDAYYLQHFTHLYTLIAA
CCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTQEPELKAYALQSAEGLAQGEIAIRHDFFAELNISTTELAATTIAPTTYHYVSHMYRQL
CCCCCCHHHHHHHHHCCCCCCCEEEEEHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
VEGTPHVAIAGMLPCPWLYQEVAQQLKQRKSPVPIYQRWIDIYSGADNADDQRAAIALID
HCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
RLYDQRMPTEQQQMLAAFRISSQMEYSFWEMAYHLEAWPKGAKVDDTKQR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKFSEKARQQVVPYWEGSFTHPFITELQAGPLDPAIFRFYLIQDAYYLQHFTHLYTLIAA
CCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTQEPELKAYALQSAEGLAQGEIAIRHDFFAELNISTTELAATTIAPTTYHYVSHMYRQL
CCCCCCHHHHHHHHHCCCCCCCEEEEEHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
VEGTPHVAIAGMLPCPWLYQEVAQQLKQRKSPVPIYQRWIDIYSGADNADDQRAAIALID
HCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
RLYDQRMPTEQQQMLAAFRISSQMEYSFWEMAYHLEAWPKGAKVDDTKQR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]