BacMap

Definition	Lactobacillus casei ATCC 334, complete genome.
Accession	NC_008526
Length	2,895,264

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The map label for this gene is braF [H]

Identifier: 116493836

GI number: 116493836

Start: 269936

End: 270721

Strand: Direct

Name: braF [H]

Synonym: LSEI_0268

Alternate gene names: 116493836

Gene position: 269936-270721 (Clockwise)

Preceding gene: 116493835

Following gene: 116493837

Centisome position: 9.32

GC content: 48.09

Gene sequence:

>786_bases
ATGACGCAACAATTGGTAATTGATCACGTCAGTAAAGTTTTTGGCGGTTTAAAAGCCGTCGCGGATGTCACGATGACGAT
CGAACAAGGGACACTGATTGCCTTGATCGGGCCAAATGGCGCTGGGAAAACAACATTGTTCAATATGTTGACTGGCGAAT
CTGGGCCGTCGTCTGGCACGATTACCTTGTATACGGCTGATGGACCAGTTGATATTACGAAAAAGAAAGCCTATCAGGTG
GCGAAGCTCGGTGTTGCTCGAACTTTTCAAAATATTCGCTTGTTTGGCGCCATGACAGTCAAAGAAAATGTTTTAGTGGC
CAGGACACATCTTTATCGTGAAAGTTTCATTGGCACGATGCTGCGGTTGCCGCAGTTTTATCAACAGGAGGCAGCCGTAG
CAACTGCAGCCGACGAATTGTTAGATTTTTTTGAGCTTGCTGATGTGGCCGATGAACCGACTGCTAGTTTGCCATATGGT
ACGCAACGGCGGGTGGAGATAGTGCGGGCAGTGGCCACGAAGCCGCAACTGCTGTTTCTGGATGAACCAGCTGCCGGGAT
GAATCCAGAAGAAACGGCAGATCTGGGGCGTTTGATTACTCGGATTCGTGAGCATTTTGGCATCACGGTGGTTTTGATTG
AACATGACATGTCGCTGGTGATGAAATTGGCTGAGTCAATTTATGTACTCGACCATGGCGCCATGATTGCTTCTGGAACC
CCTAATGAGATTCAAACTAACGCGCAGGTCATTAGTGCTTATCTGGGAGGTGAAAACCATGCTTGA

Upstream 100 bases:

>100_bases
TGGCACTCATTTTGCTAATGGTTTTCAAACCAGCTGGCTTATTAGGCCATTATGAGTTGTCGTTCAAGCGCAAGCGCCAG
ATTCGGAAAGGAGCAGCATC

Downstream 100 bases:

>100_bases
GGTTAAACATCTCGTTGTGAACTATGGTGGCATTCAAGCTGTCAAAGACGTCAGCTTTAGCGTTCAAACAGGTGAGATTG
TCGCCTTGATTGGCGCAAAT

Product: ABC-type branched-chain amino acid transport system, ATPase component

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTQQLVIDHVSKVFGGLKAVADVTMTIEQGTLIALIGPNGAGKTTLFNMLTGESGPSSGTITLYTADGPVDITKKKAYQV
AKLGVARTFQNIRLFGAMTVKENVLVARTHLYRESFIGTMLRLPQFYQQEAAVATAADELLDFFELADVADEPTASLPYG
TQRRVEIVRAVATKPQLLFLDEPAAGMNPEETADLGRLITRIREHFGITVVLIEHDMSLVMKLAESIYVLDHGAMIASGT
PNEIQTNAQVISAYLGGENHA

Sequences:

>Translated_261_residues
MTQQLVIDHVSKVFGGLKAVADVTMTIEQGTLIALIGPNGAGKTTLFNMLTGESGPSSGTITLYTADGPVDITKKKAYQV
AKLGVARTFQNIRLFGAMTVKENVLVARTHLYRESFIGTMLRLPQFYQQEAAVATAADELLDFFELADVADEPTASLPYG
TQRRVEIVRAVATKPQLLFLDEPAAGMNPEETADLGRLITRIREHFGITVVLIEHDMSLVMKLAESIYVLDHGAMIASGT
PNEIQTNAQVISAYLGGENHA
>Mature_260_residues
TQQLVIDHVSKVFGGLKAVADVTMTIEQGTLIALIGPNGAGKTTLFNMLTGESGPSSGTITLYTADGPVDITKKKAYQVA
KLGVARTFQNIRLFGAMTVKENVLVARTHLYRESFIGTMLRLPQFYQQEAAVATAADELLDFFELADVADEPTASLPYGT
QRRVEIVRAVATKPQLLFLDEPAAGMNPEETADLGRLITRIREHFGITVVLIEHDMSLVMKLAESIYVLDHGAMIASGTP
NEIQTNAQVISAYLGGENHA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI31657092, Length=238, Percent_Identity=28.5714285714286, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI30795238, Length=228, Percent_Identity=28.0701754385965, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI27881501, Length=228, Percent_Identity=28.0701754385965, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI45446740, Length=239, Percent_Identity=26.7782426778243, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI47078218, Length=239, Percent_Identity=26.7782426778243, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI150417984, Length=244, Percent_Identity=27.8688524590164, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI27477115, Length=193, Percent_Identity=31.6062176165803, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI116734710, Length=247, Percent_Identity=27.9352226720648, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI21536376, Length=237, Percent_Identity=27.0042194092827, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI105990541, Length=239, Percent_Identity=27.6150627615063, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI11967969, Length=227, Percent_Identity=22.4669603524229, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1789864, Length=257, Percent_Identity=43.1906614785992, Blast_Score=214, Evalue=4e-57,
Organism=Escherichia coli, GI1789593, Length=255, Percent_Identity=32.156862745098, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI87082268, Length=253, Percent_Identity=32.4110671936759, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI87081791, Length=244, Percent_Identity=31.5573770491803, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1788761, Length=238, Percent_Identity=29.4117647058824, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788450, Length=238, Percent_Identity=30.2521008403361, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI87081709, Length=263, Percent_Identity=26.615969581749, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1787089, Length=236, Percent_Identity=29.6610169491525, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1787370, Length=235, Percent_Identity=30.6382978723404, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI48995001, Length=254, Percent_Identity=27.9527559055118, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1787029, Length=249, Percent_Identity=28.1124497991968, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI48994943, Length=233, Percent_Identity=29.1845493562232, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788165, Length=254, Percent_Identity=27.9527559055118, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI87081782, Length=239, Percent_Identity=27.6150627615063, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787712, Length=263, Percent_Identity=24.3346007604563, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787792, Length=245, Percent_Identity=30.2040816326531, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786398, Length=246, Percent_Identity=26.4227642276423, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1790525, Length=249, Percent_Identity=24.4979919678715, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1788225, Length=238, Percent_Identity=28.9915966386555, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790467, Length=238, Percent_Identity=26.4705882352941, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1790190, Length=237, Percent_Identity=27.0042194092827, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1786345, Length=222, Percent_Identity=30.1801801801802, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI1789891, Length=217, Percent_Identity=27.6497695852535, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789672, Length=239, Percent_Identity=22.5941422594142, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789991, Length=238, Percent_Identity=27.3109243697479, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786563, Length=231, Percent_Identity=29.8701298701299, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790544, Length=250, Percent_Identity=26.4, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1786872, Length=232, Percent_Identity=25.4310344827586, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786803, Length=243, Percent_Identity=27.5720164609054, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1789586, Length=227, Percent_Identity=27.7533039647577, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789032, Length=232, Percent_Identity=25.8620689655172, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790739, Length=242, Percent_Identity=26.8595041322314, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788472, Length=232, Percent_Identity=25.8620689655172, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788897, Length=252, Percent_Identity=28.1746031746032, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786319, Length=244, Percent_Identity=25, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789873, Length=232, Percent_Identity=30.6034482758621, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786703, Length=234, Percent_Identity=26.0683760683761, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786253, Length=233, Percent_Identity=27.4678111587983, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787548, Length=243, Percent_Identity=26.3374485596708, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788644, Length=252, Percent_Identity=26.5873015873016, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87082267, Length=241, Percent_Identity=24.4813278008299, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790162, Length=249, Percent_Identity=24.8995983935743, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1789962, Length=237, Percent_Identity=22.7848101265823, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787759, Length=238, Percent_Identity=27.7310924369748, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787164, Length=231, Percent_Identity=25.1082251082251, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788053, Length=214, Percent_Identity=25.7009345794392, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87081834, Length=211, Percent_Identity=23.696682464455, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1787112, Length=228, Percent_Identity=27.1929824561404, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787105, Length=214, Percent_Identity=27.1028037383178, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI2367384, Length=59, Percent_Identity=44.0677966101695, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787143, Length=230, Percent_Identity=27.3913043478261, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71983510, Length=243, Percent_Identity=28.8065843621399, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71983516, Length=243, Percent_Identity=28.8065843621399, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71983523, Length=243, Percent_Identity=28.8065843621399, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI115533608, Length=251, Percent_Identity=28.2868525896414, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17565586, Length=227, Percent_Identity=29.9559471365639, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17510237, Length=230, Percent_Identity=26.0869565217391, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115534520, Length=247, Percent_Identity=24.2914979757085, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6319856, Length=246, Percent_Identity=26.0162601626016, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24643674, Length=245, Percent_Identity=28.1632653061224, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24666092, Length=234, Percent_Identity=28.6324786324786, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28573573, Length=251, Percent_Identity=27.4900398406374, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI28573571, Length=251, Percent_Identity=27.4900398406374, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI221512771, Length=232, Percent_Identity=25.8620689655172, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648314, Length=234, Percent_Identity=26.0683760683761, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24581191, Length=218, Percent_Identity=27.5229357798165, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581189, Length=218, Percent_Identity=27.5229357798165, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581193, Length=218, Percent_Identity=27.5229357798165, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581195, Length=218, Percent_Identity=27.5229357798165, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI85724864, Length=223, Percent_Identity=23.3183856502242, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24580555, Length=223, Percent_Identity=23.3183856502242, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI85816269, Length=223, Percent_Identity=23.3183856502242, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24580553, Length=223, Percent_Identity=23.3183856502242, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161076682, Length=226, Percent_Identity=21.6814159292035, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI85725262, Length=252, Percent_Identity=24.6031746031746, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI161076684, Length=226, Percent_Identity=21.6814159292035, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24581617, Length=226, Percent_Identity=21.6814159292035, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24581615, Length=226, Percent_Identity=21.6814159292035, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 28251; Mature: 28120

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQLVIDHVSKVFGGLKAVADVTMTIEQGTLIALIGPNGAGKTTLFNMLTGESGPSSGT
CCCHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEECCCCCCCHHHHHHHCCCCCCCCCE
ITLYTADGPVDITKKKAYQVAKLGVARTFQNIRLFGAMTVKENVLVARTHLYRESFIGTM
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHH
LRLPQFYQQEAAVATAADELLDFFELADVADEPTASLPYGTQRRVEIVRAVATKPQLLFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEE
DEPAAGMNPEETADLGRLITRIREHFGITVVLIEHDMSLVMKLAESIYVLDHGAMIASGT
ECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHEEEEECCCEEECCC
PNEIQTNAQVISAYLGGENHA
CCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TQQLVIDHVSKVFGGLKAVADVTMTIEQGTLIALIGPNGAGKTTLFNMLTGESGPSSGT
CCHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEECCCCCCCHHHHHHHCCCCCCCCCE
ITLYTADGPVDITKKKAYQVAKLGVARTFQNIRLFGAMTVKENVLVARTHLYRESFIGTM
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHH
LRLPQFYQQEAAVATAADELLDFFELADVADEPTASLPYGTQRRVEIVRAVATKPQLLFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEE
DEPAAGMNPEETADLGRLITRIREHFGITVVLIEHDMSLVMKLAESIYVLDHGAMIASGT
ECCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHEEEEECCCEEECCC
PNEIQTNAQVISAYLGGENHA
CCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]