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The map label for this gene is livK [C]

Identifier: 116493833

GI number: 116493833

Start: 266877

End: 268070

Strand: Direct

Name: livK [C]

Synonym: LSEI_0265

Alternate gene names: 116493833

Gene position: 266877-268070 (Clockwise)

Preceding gene: 116493831

Following gene: 116493834

Centisome position: 9.22

GC content: 47.82

Gene sequence:

>1194_bases
ATGAAGAAGCAAATTGTCAAAGCAATTATGAGCGCTGGCGTCATCGCAGCTTCGATGCTGGCATTGGCAGGCTGTGCGAC
AAAGAGTGCCACGAGCAAAGGAAATACGGCAGGTAACACGATTAAAATCGGCGTGAACATGGAGTTGTCTGGCGCGGTTG
CTGGTTACGGCGAACAGCAGAAACAAGGCATTGAATTGGCCGTGAAACAGATTAACGCTGCAGGTGGTATTAAGGTCGGA
AATACTAAGAAAAAAATAACGGCGGTCTATCGTGACAACAAATCGTCAACGAGCGGCGCGGCTTCAGTAGCTGCACAGCT
GGCGAATACTGACAAAGTGGTTGCGATCGTTGGACCGGCGACAACCAATGACGGAACAGCCTCTATTCCAAACGTGACCA
AGGCGGCCGTACCAATGGTGAGTCCCAGTGCTACGGATCCAAACTTTACACTTCAGAAAAATGGCAGCGTGCAACCCTAC
GTTTTCCGTGCTTGCTTCAATAACGATTTTCAAGGAGACCATGCGGCGATATTTGCCAACGATACCTTGAAGGCCAAAAA
CGTGGCGATTATTGCTGACAACTCGACTGATTACGGAACCGGGTTGGCTAAGGCATTCAAGAAGAAGTTTAAAGGTAATA
TTGTGACGACTCAGTATTATCAATCAGGTGATAAAGATTTCAATGCTATGCTGACAACGATCAAAAGCAAGAAAATTGAT
GCCATCTATGCGCCGGGCTATTATTCAGAATTAGGCCTCGTCATCAAGCAAGCCCGGCAGTCAGGCATCAAGGTACCGTT
TATTGGTGCAGATGGCATGGCCGATCCTAAGTTGGCACAAATCGCCGGCGCTGCGAATGCAACTGATATTTATTACACCA
CGCCATTTTCGACGCTGAGTGCTAACAGCAGCAAGCTGGTGAAGAACTTTATGACCAGCTTTAAGAAGCAGTATCAAACA
GAAGCACCAACGTTCTCAGCACTTGCCTATGATTCGGTTTACATGATCAAGCAAGCGATTGAAGATCAGAAGGCTGCCGA
TTCAGCCAGCATTGCCAAGGGCTTGGCCAAGATCAAACAAATGCCTGCTGTGACAGGCACCACGACCATTGATAGTCACC
ATAATCCTGAGAAGCCGCTGGCGGTTGAGCAACTGACACAAGGCAAAATCGCCAAAGTCTATACCGTTAAGTAA

Upstream 100 bases:

>100_bases
ATGGGGCGTACGATTAGCACCCATTCGTCTGAATGGGTGCTTTTTTCTGTTAAGAATCAATGAGATATAGTCATAAGCAA
TATAATGAGGGGGAACCAAG

Downstream 100 bases:

>100_bases
ATGACTGCGGAAGCTTTTTATGAGGAGATCATATTTTGCAAACCTTGATGCAACAAATCATTAATGGACTCTCACTTGGC
AGTATTTACGCTTTACTCGC

Product: ABC-type branched-chain amino acid transport system, periplasmic component

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKKQIVKAIMSAGVIAASMLALAGCATKSATSKGNTAGNTIKIGVNMELSGAVAGYGEQQKQGIELAVKQINAAGGIKVG
NTKKKITAVYRDNKSSTSGAASVAAQLANTDKVVAIVGPATTNDGTASIPNVTKAAVPMVSPSATDPNFTLQKNGSVQPY
VFRACFNNDFQGDHAAIFANDTLKAKNVAIIADNSTDYGTGLAKAFKKKFKGNIVTTQYYQSGDKDFNAMLTTIKSKKID
AIYAPGYYSELGLVIKQARQSGIKVPFIGADGMADPKLAQIAGAANATDIYYTTPFSTLSANSSKLVKNFMTSFKKQYQT
EAPTFSALAYDSVYMIKQAIEDQKAADSASIAKGLAKIKQMPAVTGTTTIDSHHNPEKPLAVEQLTQGKIAKVYTVK

Sequences:

>Translated_397_residues
MKKQIVKAIMSAGVIAASMLALAGCATKSATSKGNTAGNTIKIGVNMELSGAVAGYGEQQKQGIELAVKQINAAGGIKVG
NTKKKITAVYRDNKSSTSGAASVAAQLANTDKVVAIVGPATTNDGTASIPNVTKAAVPMVSPSATDPNFTLQKNGSVQPY
VFRACFNNDFQGDHAAIFANDTLKAKNVAIIADNSTDYGTGLAKAFKKKFKGNIVTTQYYQSGDKDFNAMLTTIKSKKID
AIYAPGYYSELGLVIKQARQSGIKVPFIGADGMADPKLAQIAGAANATDIYYTTPFSTLSANSSKLVKNFMTSFKKQYQT
EAPTFSALAYDSVYMIKQAIEDQKAADSASIAKGLAKIKQMPAVTGTTTIDSHHNPEKPLAVEQLTQGKIAKVYTVK
>Mature_397_residues
MKKQIVKAIMSAGVIAASMLALAGCATKSATSKGNTAGNTIKIGVNMELSGAVAGYGEQQKQGIELAVKQINAAGGIKVG
NTKKKITAVYRDNKSSTSGAASVAAQLANTDKVVAIVGPATTNDGTASIPNVTKAAVPMVSPSATDPNFTLQKNGSVQPY
VFRACFNNDFQGDHAAIFANDTLKAKNVAIIADNSTDYGTGLAKAFKKKFKGNIVTTQYYQSGDKDFNAMLTTIKSKKID
AIYAPGYYSELGLVIKQARQSGIKVPFIGADGMADPKLAQIAGAANATDIYYTTPFSTLSANSSKLVKNFMTSFKKQYQT
EAPTFSALAYDSVYMIKQAIEDQKAADSASIAKGLAKIKQMPAVTGTTTIDSHHNPEKPLAVEQLTQGKIAKVYTVK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=357, Percent_Identity=30.812324929972, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789867, Length=288, Percent_Identity=31.5972222222222, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41746; Mature: 41746

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQIVKAIMSAGVIAASMLALAGCATKSATSKGNTAGNTIKIGVNMELSGAVAGYGEQQ
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCHHH
KQGIELAVKQINAAGGIKVGNTKKKITAVYRDNKSSTSGAASVAAQLANTDKVVAIVGPA
HHHHHHHHHHHHCCCCEEECCCHHEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCC
TTNDGTASIPNVTKAAVPMVSPSATDPNFTLQKNGSVQPYVFRACFNNDFQGDHAAIFAN
CCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCCCCCEEEEEEC
DTLKAKNVAIIADNSTDYGTGLAKAFKKKFKGNIVTTQYYQSGDKDFNAMLTTIKSKKID
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEHHHHCCCCHHHHHHHHHHCCCCC
AIYAPGYYSELGLVIKQARQSGIKVPFIGADGMADPKLAQIAGAANATDIYYTTPFSTLS
EEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCCCCCEEEEECCCHHCC
ANSSKLVKNFMTSFKKQYQTEAPTFSALAYDSVYMIKQAIEDQKAADSASIAKGLAKIKQ
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
MPAVTGTTTIDSHHNPEKPLAVEQLTQGKIAKVYTVK
CCCCCCCEEECCCCCCCCCHHHHHHCCCCEEEEEEEC
>Mature Secondary Structure
MKKQIVKAIMSAGVIAASMLALAGCATKSATSKGNTAGNTIKIGVNMELSGAVAGYGEQQ
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCHHH
KQGIELAVKQINAAGGIKVGNTKKKITAVYRDNKSSTSGAASVAAQLANTDKVVAIVGPA
HHHHHHHHHHHHCCCCEEECCCHHEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCC
TTNDGTASIPNVTKAAVPMVSPSATDPNFTLQKNGSVQPYVFRACFNNDFQGDHAAIFAN
CCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCCCCCEEEEEEC
DTLKAKNVAIIADNSTDYGTGLAKAFKKKFKGNIVTTQYYQSGDKDFNAMLTTIKSKKID
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEHHHHCCCCHHHHHHHHHHCCCCC
AIYAPGYYSELGLVIKQARQSGIKVPFIGADGMADPKLAQIAGAANATDIYYTTPFSTLS
EEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCCCCCEEEEECCCHHCC
ANSSKLVKNFMTSFKKQYQTEAPTFSALAYDSVYMIKQAIEDQKAADSASIAKGLAKIKQ
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
MPAVTGTTTIDSHHNPEKPLAVEQLTQGKIAKVYTVK
CCCCCCCEEECCCCCCCCCHHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]