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The map label for this gene is gidB

Identifier: 116493781

GI number: 116493781

Start: 205622

End: 206353

Strand: Direct

Name: gidB

Synonym: LSEI_0210

Alternate gene names: 116493781

Gene position: 205622-206353 (Clockwise)

Preceding gene: 116493778

Following gene: 116493782

Centisome position: 7.1

GC content: 49.45

Gene sequence:

>732_bases
ATGGATCCAGCAGCTTTTGTCCAAGCCTTGGCTGATCACGGGATCGTTTTAAACGATCACCAACAGGATCAGTTTGCGGC
ATACTATCAGTATCTGATCAGTGAGAATGAAAAAATGAATCTGACTGGCATTACAGCTGAAGGGGACGTCTACCTCAAAC
ACTTCTACGATTCACTAACGCTTGCGTTGGTGCTACCTGAGCTTCAGACACAAGTAATGTCAGTTTGTGATGTGGGTGCT
GGGGCAGGTTTCCCATCAATTCCGTTAAAAATTGCGTTCCCGCAACTCAAAATCACCATCGTGGATTCTTTACAAAAGCG
AATTGGCTTTCTCGAACGATTAACGGCGCGTTTGGAGCTGACAGATGTTCAGCTCTTTCATGACCGCGCCGAAGCTTTTG
GTGCCAAAAAATCGCCACACCGTGCCAGTTTTGATTTGGTCACGGCTCGTGCGGTTGCGGCATTGGATGTCTTGGCTGAG
CTCTGTTTGCCACTAGTAAAGCCGCAAGGTCGCTTTGTGGCCATGAAAGCCGCGGCGACACCCGCAGAATTAATTGCGGC
CAAGTCAGCGATTGGCTTACTTGGCGGCAAATTGGCCCAAGATGCTGCGTTGACGTTGCCGGAAACCGGCGATCCACGTC
ATTTGCTGGTCATCGACAAAGTTAAACCAACACCAAACAAATATCCGCGCAAGCCGGGGATACCGAATAAGCAACCTTTG
GGAGGTGCATAA

Upstream 100 bases:

>100_bases
ATAAAGACGGTTGAGCAGAGGCCGCTTTTACGTTACACTTGACGGGTACGTGAAACCCTGTTTGTTCCACAAAATCAAGT
TTACGAAAATGGAGGCAGGC

Downstream 100 bases:

>100_bases
CGCGTGCCATTATCATCCATTTTTGGCCATAAAAAGCCTGACGACGAAACGGTTGTTCAACAACTGCCAATAACCGCAAT
CGTGCCTAACCAATTCCAGC

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MDPAAFVQALADHGIVLNDHQQDQFAAYYQYLISENEKMNLTGITAEGDVYLKHFYDSLTLALVLPELQTQVMSVCDVGA
GAGFPSIPLKIAFPQLKITIVDSLQKRIGFLERLTARLELTDVQLFHDRAEAFGAKKSPHRASFDLVTARAVAALDVLAE
LCLPLVKPQGRFVAMKAAATPAELIAAKSAIGLLGGKLAQDAALTLPETGDPRHLLVIDKVKPTPNKYPRKPGIPNKQPL
GGA

Sequences:

>Translated_243_residues
MDPAAFVQALADHGIVLNDHQQDQFAAYYQYLISENEKMNLTGITAEGDVYLKHFYDSLTLALVLPELQTQVMSVCDVGA
GAGFPSIPLKIAFPQLKITIVDSLQKRIGFLERLTARLELTDVQLFHDRAEAFGAKKSPHRASFDLVTARAVAALDVLAE
LCLPLVKPQGRFVAMKAAATPAELIAAKSAIGLLGGKLAQDAALTLPETGDPRHLLVIDKVKPTPNKYPRKPGIPNKQPL
GGA
>Mature_243_residues
MDPAAFVQALADHGIVLNDHQQDQFAAYYQYLISENEKMNLTGITAEGDVYLKHFYDSLTLALVLPELQTQVMSVCDVGA
GAGFPSIPLKIAFPQLKITIVDSLQKRIGFLERLTARLELTDVQLFHDRAEAFGAKKSPHRASFDLVTARAVAALDVLAE
LCLPLVKPQGRFVAMKAAATPAELIAAKSAIGLLGGKLAQDAALTLPETGDPRHLLVIDKVKPTPNKYPRKPGIPNKQPL
GGA

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=211, Percent_Identity=35.0710900473934, Blast_Score=111, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_LACC3 (Q03CJ9)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_805515.1
- ProteinModelPortal:   Q03CJ9
- SMR:   Q03CJ9
- STRING:   Q03CJ9
- GeneID:   4420297
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_0210
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   HLWDSLR
- PhylomeDB:   Q03CJ9
- ProtClustDB:   PRK00107
- BioCyc:   LCAS321967:LSEI_0210-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 26231; Mature: 26231

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPAAFVQALADHGIVLNDHQQDQFAAYYQYLISENEKMNLTGITAEGDVYLKHFYDSLT
CCHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHH
LALVLPELQTQVMSVCDVGAGAGFPSIPLKIAFPQLKITIVDSLQKRIGFLERLTARLEL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEHHHHHHHHHHHHHHHHHHHHHH
TDVQLFHDRAEAFGAKKSPHRASFDLVTARAVAALDVLAELCLPLVKPQGRFVAMKAAAT
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
PAELIAAKSAIGLLGGKLAQDAALTLPETGDPRHLLVIDKVKPTPNKYPRKPGIPNKQPL
HHHHHHHHHHHHHHCCHHHCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
GGA
CCC
>Mature Secondary Structure
MDPAAFVQALADHGIVLNDHQQDQFAAYYQYLISENEKMNLTGITAEGDVYLKHFYDSLT
CCHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHH
LALVLPELQTQVMSVCDVGAGAGFPSIPLKIAFPQLKITIVDSLQKRIGFLERLTARLEL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEHHHHHHHHHHHHHHHHHHHHHH
TDVQLFHDRAEAFGAKKSPHRASFDLVTARAVAALDVLAELCLPLVKPQGRFVAMKAAAT
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
PAELIAAKSAIGLLGGKLAQDAALTLPETGDPRHLLVIDKVKPTPNKYPRKPGIPNKQPL
HHHHHHHHHHHHHHCCHHHCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
GGA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA