Click here to switch to the map view.

The map label for this gene is bsdA [H]

Identifier: 116493698

GI number: 116493698

Start: 124755

End: 125633

Strand: Direct

Name: bsdA [H]

Synonym: LSEI_0126

Alternate gene names: 116493698

Gene position: 124755-125633 (Clockwise)

Preceding gene: 116493696

Following gene: 116493699

Centisome position: 4.31

GC content: 54.15

Gene sequence:

>879_bases
ATGAATCTCAAGCAAATTACCTATTTTTTAGCGGTAGCAGAGGCGGGCCAGATGACTGCGGCGGCGAAACAATTGCACAT
GGCGCAGCCGCCGTTGAGTTATCAATTGAAAGCACTTGAAGATGAACTGGGTGTACGCCTGTTTGAACGTGGGTCGCGCG
GTGTCCGACTCACTGGTAGCGGCCAACAGTTTATGACATACGCACAACAGATCGTTGAGACGGTTGCGGCAGCTGAGAAC
GCAGCGCGGCGTAGCGGTGAAGGTGAGATTGGCACACTGGCGCTGGGACTGACTTCTTCGGCCGGTGATGTCCTGCCGAA
GCAAAAATTGCAGGCCTTTAATCGCCGCTATCCGCAAGTCGAGTTTGCGCTTTATGAGGACAACACTTATGGCATCCTCG
ACAAGTTACGCCGCAACATTCTGGATTTGGCCATCGTCCGAACGCCATTTAATGATGACGGTCTAGCTTCATGTCGCCTA
AGCAGTGACCACATGATCGCCGTTGCTGCCGCGTCGCAGCCCTTGCCAGCCACGCTAACCATTCCCGACCTCAAGCAGCA
GCCGCTGATTGTGTATCGCCGGTTTAGTTCGCTATTCAAAACCACTTTCGCCGAGTATGGCATCACACCGATAATTGCTG
TGTTGTGTGATGACGCGCGCACGGCGTTGCATTGGGCCCAGATGGGGATGGGGGTGGCGTTGGTACCGGCGACCATGGCC
GCTTTGGCGAGTCAGCAAAGTCACCTTGCCGTGATTGATTATGAGCCATGGGTGACGCACATGACGCTCGTTTGGCAGCC
TGAGGCGTTGCACAATCCATTGGTGGCAAGATTTGTGGGGCAACTTTCGGGTGGGGAATCGGAGAAATATTCAAAATAA

Upstream 100 bases:

>100_bases
ATATAAAAAATACATATTCGAGGTACTATCCATACAATTTGTGTATAGTGGAGCTGAAGGATTGCGAAGCTGACGATTAA
AAATGAAGGGATCAATCGCG

Downstream 100 bases:

>100_bases
GGTTCGATTAGTTTTGTAGAAAGAGTCCTGAGCCGTGCCAAACTCCGCAATCTCCGGCCAGCTGGGGCTGGAACGCTGTG
ACGGCGCTTCGAGCCCGCAA

Product: fhu operon transcription regulator

Products: NA

Alternate protein names: Bsd operon regulatory protein [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MNLKQITYFLAVAEAGQMTAAAKQLHMAQPPLSYQLKALEDELGVRLFERGSRGVRLTGSGQQFMTYAQQIVETVAAAEN
AARRSGEGEIGTLALGLTSSAGDVLPKQKLQAFNRRYPQVEFALYEDNTYGILDKLRRNILDLAIVRTPFNDDGLASCRL
SSDHMIAVAAASQPLPATLTIPDLKQQPLIVYRRFSSLFKTTFAEYGITPIIAVLCDDARTALHWAQMGMGVALVPATMA
ALASQQSHLAVIDYEPWVTHMTLVWQPEALHNPLVARFVGQLSGGESEKYSK

Sequences:

>Translated_292_residues
MNLKQITYFLAVAEAGQMTAAAKQLHMAQPPLSYQLKALEDELGVRLFERGSRGVRLTGSGQQFMTYAQQIVETVAAAEN
AARRSGEGEIGTLALGLTSSAGDVLPKQKLQAFNRRYPQVEFALYEDNTYGILDKLRRNILDLAIVRTPFNDDGLASCRL
SSDHMIAVAAASQPLPATLTIPDLKQQPLIVYRRFSSLFKTTFAEYGITPIIAVLCDDARTALHWAQMGMGVALVPATMA
ALASQQSHLAVIDYEPWVTHMTLVWQPEALHNPLVARFVGQLSGGESEKYSK
>Mature_292_residues
MNLKQITYFLAVAEAGQMTAAAKQLHMAQPPLSYQLKALEDELGVRLFERGSRGVRLTGSGQQFMTYAQQIVETVAAAEN
AARRSGEGEIGTLALGLTSSAGDVLPKQKLQAFNRRYPQVEFALYEDNTYGILDKLRRNILDLAIVRTPFNDDGLASCRL
SSDHMIAVAAASQPLPATLTIPDLKQQPLIVYRRFSSLFKTTFAEYGITPIIAVLCDDARTALHWAQMGMGVALVPATMA
ALASQQSHLAVIDYEPWVTHMTLVWQPEALHNPLVARFVGQLSGGESEKYSK

Specific function: Could be a positive regulator of bsdBCD expression in response to salicylic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=286, Percent_Identity=30.0699300699301, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787806, Length=283, Percent_Identity=30.7420494699647, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI157672245, Length=281, Percent_Identity=29.1814946619217, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788887, Length=256, Percent_Identity=31.25, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1790399, Length=286, Percent_Identity=26.9230769230769, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI2367136, Length=248, Percent_Identity=28.6290322580645, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787530, Length=277, Percent_Identity=26.3537906137184, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788297, Length=245, Percent_Identity=26.9387755102041, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787601, Length=145, Percent_Identity=31.7241379310345, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787949, Length=169, Percent_Identity=29.585798816568, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789204, Length=296, Percent_Identity=27.3648648648649, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790262, Length=241, Percent_Identity=25.7261410788382, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790208, Length=292, Percent_Identity=23.972602739726, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788748, Length=148, Percent_Identity=27.027027027027, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1789639, Length=254, Percent_Identity=27.9527559055118, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081904, Length=148, Percent_Identity=30.4054054054054, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082132, Length=222, Percent_Identity=25.2252252252252, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788296, Length=253, Percent_Identity=24.5059288537549, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693105, Length=289, Percent_Identity=21.7993079584775, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789506, Length=243, Percent_Identity=23.0452674897119, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786401, Length=186, Percent_Identity=27.9569892473118, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786713, Length=173, Percent_Identity=27.7456647398844, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32011; Mature: 32011

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKQITYFLAVAEAGQMTAAAKQLHMAQPPLSYQLKALEDELGVRLFERGSRGVRLTGS
CCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECC
GQQFMTYAQQIVETVAAAENAARRSGEGEIGTLALGLTSSAGDVLPKQKLQAFNRRYPQV
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCCCE
EFALYEDNTYGILDKLRRNILDLAIVRTPFNDDGLASCRLSSDHMIAVAAASQPLPATLT
EEEEEECCCHHHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCEEEEEECCCCCCEEEE
IPDLKQQPLIVYRRFSSLFKTTFAEYGITPIIAVLCDDARTALHWAQMGMGVALVPATMA
CCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHH
ALASQQSHLAVIDYEPWVTHMTLVWQPEALHNPLVARFVGQLSGGESEKYSK
HHHCCCCCEEEEECCCCCEEEEEEECCHHHHCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNLKQITYFLAVAEAGQMTAAAKQLHMAQPPLSYQLKALEDELGVRLFERGSRGVRLTGS
CCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECC
GQQFMTYAQQIVETVAAAENAARRSGEGEIGTLALGLTSSAGDVLPKQKLQAFNRRYPQV
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCCCE
EFALYEDNTYGILDKLRRNILDLAIVRTPFNDDGLASCRLSSDHMIAVAAASQPLPATLT
EEEEEECCCHHHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCEEEEEECCCCCCEEEE
IPDLKQQPLIVYRRFSSLFKTTFAEYGITPIIAVLCDDARTALHWAQMGMGVALVPATMA
CCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHH
ALASQQSHLAVIDYEPWVTHMTLVWQPEALHNPLVARFVGQLSGGESEKYSK
HHHCCCCCEEEEECCCCCEEEEEEECCHHHHCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]