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The map label for this gene is flp [H]

Identifier: 116493645

GI number: 116493645

Start: 68632

End: 69291

Strand: Direct

Name: flp [H]

Synonym: LSEI_0073

Alternate gene names: 116493645

Gene position: 68632-69291 (Clockwise)

Preceding gene: 116493644

Following gene: 116493651

Centisome position: 2.37

GC content: 51.21

Gene sequence:

>660_bases
ATGACGCACAGTTGTACGGCGGTAGTGCCGTTATTCAAAAATTTAAACGATGAGGCGCGTGCCGCCATTGACGCGTTGAC
GCACGAACGCCAGGTTGAAAAAGGAACTGTTTTGATCAGCCCTGATACGGCCGCCCATTTGTTGGTGGTTGCGCACGGCA
AACTCAAAACTTACCAGTTGGCCACCAATGGCAAGGAACAGCTACTACGGGTTGATGGTATTGGTGAGTATGAAGGTGAA
GCCGGCTTACTCAATATCGCTAATCCGAATGTCTATACCGAAACCCTGACGGCGGCAACTGTCTGCACCATCAGTGCCAC
TGACTTTCAACAATTGCTTCTCAAGCAGCCGCAAATCAGTCTCCAACTCCTGACTGAAAACGCCCGTAAAATGCAGGCGC
TGGAAAAACAGGCCGGCTATTTGGGTAACGATTCGATCAACGTTCGGCTCACCCACTACCTACTGGACTTGCGTACCGCG
GCGGGCCAAGACACCGTCACGGTCCCGATGGCATGGACACAATTGGCCGATTATCTCGGAACAACGCCGGAAACGGTCTC
GCGCACTTTGAAACGACTCGCCGAGGAAAAACTCATTGAACGTTCAGGTAAGCAGGTAAGAATTCTGAATGCTGAGGATA
TGGAAGATTTTGCTTGGTGA

Upstream 100 bases:

>100_bases
CCAAGTGACGGCGGGGCGGGCATTGCTTGACGAGGATGAAGATGACGACTGATCAAGCTTGTTGACAGAGCGTGAGCAGG
CGCGTTTAGGAGGTGGCAAC

Downstream 100 bases:

>100_bases
GGTTGCCGAGTGATGTTTTTTAAATACAAAAAGCCACGAATCATGCAATCTAAACTAATTGCACGATTCGTGGCTTTTTC
TTTGTTTTGCAAGAAGCGAC

Product: Crp-like transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MTHSCTAVVPLFKNLNDEARAAIDALTHERQVEKGTVLISPDTAAHLLVVAHGKLKTYQLATNGKEQLLRVDGIGEYEGE
AGLLNIANPNVYTETLTAATVCTISATDFQQLLLKQPQISLQLLTENARKMQALEKQAGYLGNDSINVRLTHYLLDLRTA
AGQDTVTVPMAWTQLADYLGTTPETVSRTLKRLAEEKLIERSGKQVRILNAEDMEDFAW

Sequences:

>Translated_219_residues
MTHSCTAVVPLFKNLNDEARAAIDALTHERQVEKGTVLISPDTAAHLLVVAHGKLKTYQLATNGKEQLLRVDGIGEYEGE
AGLLNIANPNVYTETLTAATVCTISATDFQQLLLKQPQISLQLLTENARKMQALEKQAGYLGNDSINVRLTHYLLDLRTA
AGQDTVTVPMAWTQLADYLGTTPETVSRTLKRLAEEKLIERSGKQVRILNAEDMEDFAW
>Mature_218_residues
THSCTAVVPLFKNLNDEARAAIDALTHERQVEKGTVLISPDTAAHLLVVAHGKLKTYQLATNGKEQLLRVDGIGEYEGEA
GLLNIANPNVYTETLTAATVCTISATDFQQLLLKQPQISLQLLTENARKMQALEKQAGYLGNDSINVRLTHYLLDLRTAA
GQDTVTVPMAWTQLADYLGTTPETVSRTLKRLAEEKLIERSGKQVRILNAEDMEDFAW

Specific function: This Protein Complexes With Cyclic AMP And Binds To Specific DNA Sites Near The Promoter To Regulate The Transcription Of Several Catabolite-Sensitive Operons. The Protein Induces A Severe Bend In The DNA. Acts As A Negative Regulator Of Its Own Synthesi

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=180, Percent_Identity=27.7777777777778, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24161; Mature: 24030

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSCTAVVPLFKNLNDEARAAIDALTHERQVEKGTVLISPDTAAHLLVVAHGKLKTYQL
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCEEEEEE
ATNGKEQLLRVDGIGEYEGEAGLLNIANPNVYTETLTAATVCTISATDFQQLLLKQPQIS
CCCCHHHEEEECCCCCCCCCCCEEEECCCCEEHHHHHHHEEEEEEHHHHHHHHHCCCCCE
LQLLTENARKMQALEKQAGYLGNDSINVRLTHYLLDLRTAAGQDTVTVPMAWTQLADYLG
EEHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHCCCCCEEEECHHHHHHHHHHC
TTPETVSRTLKRLAEEKLIERSGKQVRILNAEDMEDFAW
CCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCC
>Mature Secondary Structure 
THSCTAVVPLFKNLNDEARAAIDALTHERQVEKGTVLISPDTAAHLLVVAHGKLKTYQL
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCEEEEEE
ATNGKEQLLRVDGIGEYEGEAGLLNIANPNVYTETLTAATVCTISATDFQQLLLKQPQIS
CCCCHHHEEEECCCCCCCCCCCEEEECCCCEEHHHHHHHEEEEEEHHHHHHHHHCCCCCE
LQLLTENARKMQALEKQAGYLGNDSINVRLTHYLLDLRTAAGQDTVTVPMAWTQLADYLG
EEHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHCCCCCEEEECHHHHHHHHHHC
TTPETVSRTLKRLAEEKLIERSGKQVRILNAEDMEDFAW
CCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2113923; 8441692 [H]