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The map label for this gene is ackA1 [H]

Identifier: 116493548

GI number: 116493548

Start: 1802773

End: 1803960

Strand: Direct

Name: ackA1 [H]

Synonym: PEPE_1822

Alternate gene names: 116493548

Gene position: 1802773-1803960 (Clockwise)

Preceding gene: 116493547

Following gene: 116493549

Centisome position: 98.38

GC content: 41.08

Gene sequence:

>1188_bases
ATGAAAAAAGACTTAGTTATTAGTGCGGGCAGTTCAACTCTGAAATGGAAGTTGTTTTCGATTCCTGATGAAACAGTAAT
CGCAACTGGATTGATTGACCGAATCAATCTTCCCAACTCCGTTTTTATGGTTAAACGCCCCCATCATGATCCTTATAAAG
TAGTAAAACCTTCAATCACCTATGATTTAGCGGTTACCATGCTGCTTTCTTTCCTTAAGGATCATGGACTAGTCAAGCAT
TTACGTGAGATTACTGGAATTGGACATCGAGTTGTCGCAGGGGGCGAACACTTTAAAGACTCGGTTTTAATCGATGATGA
AGTTTTAGCCGAAATCCACAATTTAGCTGAATATGCCCCACTTCATAACCCGGTTCAGGCCCGCTACATTGAAATTTTTA
AACAGCTCCTGCCAGATGTTCGTCAAGTGGCGGTTTTCGACACTTCGTTATACGCTGATATGCCGAAAGTTAACTGCCTC
TATCCCATCCCTTACGAATACTACACAGACTTAGGGATTCGTAAATATGGTGCGCACGGGACCAGTCATCGGTATGTCGC
CAATCGGGCAGCAGATCTTCTAAAAAAAGATTTAAGCGATTTGAAAATGATTACGTGTCATCTCGGTTCCGGTTCATCAA
TTACGGCCTTTAATCAAGGACATGTAATCGATACCTCTATGGGATTCATTCCCTTAGGCGGGGTAATGATGGCGACTCGA
TCGGGAGATTTAGATCCATCACTGGTCGGTTTTCTAGCTGAAAAATTAGGGATTAGTGTTAACGAAGTGATTGATATTCT
AAATCAAAAATCAGGATTATTAGGAATTTCCCAATTATCTCCTGATCAACGGGACTTGGAAGATGCCGAAAATTCTAATC
CTCAAGCTAAGTTAGCATTGGAAATGTTCATTAACCGCGTAGTCCGTTTTATTGGTAGCTATGTGGCTGAACTTGGTGGA
TTAGATGCTCTGATTTTCACTGCTGGCTGTGGTGAAAATGGAATTGCAATGCGAGAACGCATTATGGAACAACTTACCTA
CTTCGGCATTAATGCTGATGTTGAGCGTAATAATGTACGTGGTAAAGAACAACAGATTAATCAAGTAACTGATGCGGTTC
AAGTTCTCGTTGTGCCAACTAATGAGGAATTAATGATTGCTCGCGATATCCAACGAATTTTACGTTAA

Upstream 100 bases:

>100_bases
TATCGATAGTTAAGTATTTTTGTTGGAAATCCGGTATAATTGTAAGCGGATACAAAAACTAATTTCCAATAAATCTACAC
ATGTTAGCGAGGAATATCTC

Downstream 100 bases:

>100_bases
ATTGAACTTCAACCCCCAAGCTTGTAAATAAGCTTGGGGGTTTTCTTTACTATTTTAATCATTTATAATACTATATGAGA
TATAGTATTATGGAGGTGAG

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase 1 [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MKKDLVISAGSSTLKWKLFSIPDETVIATGLIDRINLPNSVFMVKRPHHDPYKVVKPSITYDLAVTMLLSFLKDHGLVKH
LREITGIGHRVVAGGEHFKDSVLIDDEVLAEIHNLAEYAPLHNPVQARYIEIFKQLLPDVRQVAVFDTSLYADMPKVNCL
YPIPYEYYTDLGIRKYGAHGTSHRYVANRAADLLKKDLSDLKMITCHLGSGSSITAFNQGHVIDTSMGFIPLGGVMMATR
SGDLDPSLVGFLAEKLGISVNEVIDILNQKSGLLGISQLSPDQRDLEDAENSNPQAKLALEMFINRVVRFIGSYVAELGG
LDALIFTAGCGENGIAMRERIMEQLTYFGINADVERNNVRGKEQQINQVTDAVQVLVVPTNEELMIARDIQRILR

Sequences:

>Translated_395_residues
MKKDLVISAGSSTLKWKLFSIPDETVIATGLIDRINLPNSVFMVKRPHHDPYKVVKPSITYDLAVTMLLSFLKDHGLVKH
LREITGIGHRVVAGGEHFKDSVLIDDEVLAEIHNLAEYAPLHNPVQARYIEIFKQLLPDVRQVAVFDTSLYADMPKVNCL
YPIPYEYYTDLGIRKYGAHGTSHRYVANRAADLLKKDLSDLKMITCHLGSGSSITAFNQGHVIDTSMGFIPLGGVMMATR
SGDLDPSLVGFLAEKLGISVNEVIDILNQKSGLLGISQLSPDQRDLEDAENSNPQAKLALEMFINRVVRFIGSYVAELGG
LDALIFTAGCGENGIAMRERIMEQLTYFGINADVERNNVRGKEQQINQVTDAVQVLVVPTNEELMIARDIQRILR
>Mature_395_residues
MKKDLVISAGSSTLKWKLFSIPDETVIATGLIDRINLPNSVFMVKRPHHDPYKVVKPSITYDLAVTMLLSFLKDHGLVKH
LREITGIGHRVVAGGEHFKDSVLIDDEVLAEIHNLAEYAPLHNPVQARYIEIFKQLLPDVRQVAVFDTSLYADMPKVNCL
YPIPYEYYTDLGIRKYGAHGTSHRYVANRAADLLKKDLSDLKMITCHLGSGSSITAFNQGHVIDTSMGFIPLGGVMMATR
SGDLDPSLVGFLAEKLGISVNEVIDILNQKSGLLGISQLSPDQRDLEDAENSNPQAKLALEMFINRVVRFIGSYVAELGG
LDALIFTAGCGENGIAMRERIMEQLTYFGINADVERNNVRGKEQQINQVTDAVQVLVVPTNEELMIARDIQRILR

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=397, Percent_Identity=38.7909319899244, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI145693184, Length=388, Percent_Identity=40.4639175257732, Blast_Score=271, Evalue=5e-74,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43843; Mature: 43843

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDLVISAGSSTLKWKLFSIPDETVIATGLIDRINLPNSVFMVKRPHHDPYKVVKPSIT
CCCCEEEECCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHCCCCHH
YDLAVTMLLSFLKDHGLVKHLREITGIGHRVVAGGEHFKDSVLIDDEVLAEIHNLAEYAP
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEECCCCCCCCEEECHHHHHHHHHHHHHCC
LHNPVQARYIEIFKQLLPDVRQVAVFDTSLYADMPKVNCLYPIPYEYYTDLGIRKYGAHG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHCCCCHHHCCCC
TSHRYVANRAADLLKKDLSDLKMITCHLGSGSSITAFNQGHVIDTSMGFIPLGGVMMATR
CCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEECCCCEEECCCCCEECCCEEEEEC
SGDLDPSLVGFLAEKLGISVNEVIDILNQKSGLLGISQLSPDQRDLEDAENSNPQAKLAL
CCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEHHHCCCCHHHHHHHCCCCCHHHHHH
EMFINRVVRFIGSYVAELGGLDALIFTAGCGENGIAMRERIMEQLTYFGINADVERNNVR
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
GKEQQINQVTDAVQVLVVPTNEELMIARDIQRILR
CHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHC
>Mature Secondary Structure
MKKDLVISAGSSTLKWKLFSIPDETVIATGLIDRINLPNSVFMVKRPHHDPYKVVKPSIT
CCCCEEEECCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHCCCCHH
YDLAVTMLLSFLKDHGLVKHLREITGIGHRVVAGGEHFKDSVLIDDEVLAEIHNLAEYAP
HHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEECCCCCCCCEEECHHHHHHHHHHHHHCC
LHNPVQARYIEIFKQLLPDVRQVAVFDTSLYADMPKVNCLYPIPYEYYTDLGIRKYGAHG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHCCCCHHHCCCC
TSHRYVANRAADLLKKDLSDLKMITCHLGSGSSITAFNQGHVIDTSMGFIPLGGVMMATR
CCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEECCCCEEECCCCCEECCCEEEEEC
SGDLDPSLVGFLAEKLGISVNEVIDILNQKSGLLGISQLSPDQRDLEDAENSNPQAKLAL
CCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEHHHCCCCHHHHHHHCCCCCHHHHHH
EMFINRVVRFIGSYVAELGGLDALIFTAGCGENGIAMRERIMEQLTYFGINADVERNNVR
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
GKEQQINQVTDAVQVLVVPTNEELMIARDIQRILR
CHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10941799 [H]