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The map label for this gene is glpF [H]

Identifier: 116493353

GI number: 116493353

Start: 1606075

End: 1606794

Strand: Direct

Name: glpF [H]

Synonym: PEPE_1625

Alternate gene names: 116493353

Gene position: 1606075-1606794 (Clockwise)

Preceding gene: 116493352

Following gene: 116493360

Centisome position: 87.65

GC content: 43.33

Gene sequence:

>720_bases
ATGGATAATAGTTTATCGCTTCAACTACTGGGGGAGTTTATTGGAACATTTGTACTAGTTTTACTTGGTGACGGTGTTGT
GGCTGGAGTTAGCCTTAGAAAGTCAAAAGCTGAAGGCTCTGGTTGGATCGCCATTACTTTAGGCTGGGGATTAGCTGTTA
CATTAGGGGTTTATGCATCCTCTTTTCTCAGCCCTGCCCATTTAAATCCTGCAGTTTCAGTCGGAATGGCAATTGCCGGT
AAATTTCCTTGGTCAAGCGTTATTCCTTATAGCATTGCTCAAATTTTAGGTGGTTTAGTTGGTGGAATTATCGTTTGGAT
TCATTATTACCCACATTGGAAAGAAACCAAAGATAAAGATGCTATTCTTGGTACCTTTGCTACAGAACCTGCTATTCGCA
ACTACAGCATGAACTTTATTAGTGAAGTTGTTGGTACAGCAGTACTTATTTTCGGTTTATTAGCCTTTACTCGGGGACAA
TTCACTCAGGGTCTTAATCCCATCGCTGTTGGGGTTCTCATTACAGCAATCGGACTTTCACTTGGCGGAACTACCGGTTA
CGCCATCAATCCTGCGCGGGATTTAGGTCCTCGTCTCGCACATGCGATGCTTCCAATCGCTAACAAAGGCGGCTCAGATT
GGGCCTATAGTTGGGTTCCTATTTTGGGACCAGTTGTTGGTGCCATTCTAGGAGCAATTTGCTTCAATTTGATCCCTTAA

Upstream 100 bases:

>100_bases
TTCGAATGGTCTGACGAAGAGCGTGAAGCTCAAGCGAAGAAACTCCAAGAAACAATCGATGAAGCTAGCCTTACTTACCT
AAAATAGGAGGAACCTTTTC

Downstream 100 bases:

>100_bases
CAATTAACACTTTATAATTCATCGACCCTTTTTACCCTTTATGCTTAATATTAAAGCAGCAAAACACCGGAATTTAAATT
CCGGTGTTTTTATTTTTTAA

Product: glycerol uptake facilitator related permease (major Intrinsic protein family)

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MDNSLSLQLLGEFIGTFVLVLLGDGVVAGVSLRKSKAEGSGWIAITLGWGLAVTLGVYASSFLSPAHLNPAVSVGMAIAG
KFPWSSVIPYSIAQILGGLVGGIIVWIHYYPHWKETKDKDAILGTFATEPAIRNYSMNFISEVVGTAVLIFGLLAFTRGQ
FTQGLNPIAVGVLITAIGLSLGGTTGYAINPARDLGPRLAHAMLPIANKGGSDWAYSWVPILGPVVGAILGAICFNLIP

Sequences:

>Translated_239_residues
MDNSLSLQLLGEFIGTFVLVLLGDGVVAGVSLRKSKAEGSGWIAITLGWGLAVTLGVYASSFLSPAHLNPAVSVGMAIAG
KFPWSSVIPYSIAQILGGLVGGIIVWIHYYPHWKETKDKDAILGTFATEPAIRNYSMNFISEVVGTAVLIFGLLAFTRGQ
FTQGLNPIAVGVLITAIGLSLGGTTGYAINPARDLGPRLAHAMLPIANKGGSDWAYSWVPILGPVVGAILGAICFNLIP
>Mature_239_residues
MDNSLSLQLLGEFIGTFVLVLLGDGVVAGVSLRKSKAEGSGWIAITLGWGLAVTLGVYASSFLSPAHLNPAVSVGMAIAG
KFPWSSVIPYSIAQILGGLVGGIIVWIHYYPHWKETKDKDAILGTFATEPAIRNYSMNFISEVVGTAVLIFGLLAFTRGQ
FTQGLNPIAVGVLITAIGLSLGGTTGYAINPARDLGPRLAHAMLPIANKGGSDWAYSWVPILGPVVGAILGAICFNLIP

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=253, Percent_Identity=35.5731225296443, Blast_Score=131, Evalue=5e-31,
Organism=Homo sapiens, GI4826645, Length=246, Percent_Identity=36.5853658536585, Blast_Score=131, Evalue=5e-31,
Organism=Homo sapiens, GI22538420, Length=250, Percent_Identity=36.8, Blast_Score=127, Evalue=7e-30,
Organism=Homo sapiens, GI4502187, Length=249, Percent_Identity=30.9236947791165, Blast_Score=106, Evalue=2e-23,
Organism=Escherichia coli, GI1790362, Length=251, Percent_Identity=35.0597609561753, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787101, Length=195, Percent_Identity=32.3076923076923, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71994009, Length=242, Percent_Identity=33.0578512396694, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI71992966, Length=249, Percent_Identity=31.3253012048193, Blast_Score=120, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17531431, Length=242, Percent_Identity=33.8842975206612, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17531429, Length=242, Percent_Identity=33.8842975206612, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI32564052, Length=240, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17533613, Length=240, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17544068, Length=245, Percent_Identity=34.6938775510204, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71992961, Length=245, Percent_Identity=27.7551020408163, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17558372, Length=237, Percent_Identity=26.1603375527426, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71993722, Length=238, Percent_Identity=25.6302521008403, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=215, Percent_Identity=34.4186046511628, Blast_Score=111, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6322985, Length=286, Percent_Identity=27.972027972028, Blast_Score=92, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24652747, Length=234, Percent_Identity=29.4871794871795, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI45551084, Length=234, Percent_Identity=29.4871794871795, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24978; Mature: 24978

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNSLSLQLLGEFIGTFVLVLLGDGVVAGVSLRKSKAEGSGWIAITLGWGLAVTLGVYAS
CCCCHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHCCCCCCCEEEEEECHHHHHHHHHHHH
SFLSPAHLNPAVSVGMAIAGKFPWSSVIPYSIAQILGGLVGGIIVWIHYYPHWKETKDKD
HHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
AILGTFATEPAIRNYSMNFISEVVGTAVLIFGLLAFTRGQFTQGLNPIAVGVLITAIGLS
HEEEHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LGGTTGYAINPARDLGPRLAHAMLPIANKGGSDWAYSWVPILGPVVGAILGAICFNLIP
CCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDNSLSLQLLGEFIGTFVLVLLGDGVVAGVSLRKSKAEGSGWIAITLGWGLAVTLGVYAS
CCCCHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHCCCCCCCEEEEEECHHHHHHHHHHHH
SFLSPAHLNPAVSVGMAIAGKFPWSSVIPYSIAQILGGLVGGIIVWIHYYPHWKETKDKD
HHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
AILGTFATEPAIRNYSMNFISEVVGTAVLIFGLLAFTRGQFTQGLNPIAVGVLITAIGLS
HEEEHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LGGTTGYAINPARDLGPRLAHAMLPIANKGGSDWAYSWVPILGPVVGAILGAICFNLIP
CCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]