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The map label for this gene is glpK

Identifier: 116493351

GI number: 116493351

Start: 1602712

End: 1604226

Strand: Direct

Name: glpK

Synonym: PEPE_1623

Alternate gene names: 116493351

Gene position: 1602712-1604226 (Clockwise)

Preceding gene: 116493350

Following gene: 116493352

Centisome position: 87.47

GC content: 42.24

Gene sequence:

>1515_bases
ATGGCAGACAAATATATTCTTGCCATCGACGAAGGGACGACTAGTACTCGTACAATCATCTTTGATCATGCTGGAAATAA
AATGGCAGATGCTCAAAGAGAATTTCCACAGTACTTTCCGAAACCTGGATGGGTAGAACATAATGCCAACGAAATATGGA
ATGCCGTATTGTCGACCATTGCTAATGCATTTATCGAGTCAGGAATTAAGCCCAACCAAATTAGTGGAATTGGTATTACC
AATCAACGAGAAACTACCGTGATTTGGGATAAAAAGACCGGGCTTCCCATTTATAACGCTGTAGTGTGGCAATCTCGACA
GACAGCAGAAATTGCTGAACAACTCGTAAAAGATGGGTATGGCGACATGATTCACCAAAAAACGGGACTAGTTACAGACG
CTTACTTCTCGGCAACCAAAATTCGCTGGATTCTTGACCACGTTGATGGCGCACAAGCACGCGCCGAACGCGGCGAGCTG
TTGTTTGGAACCATTGATACATGGCTAATGTGGAAATTAACGGATGGTGATGTGCACGTCACTGACTATTCCAACGCTAG
CCGCACCATGTTATATAACATTCACAAATTAGAATGGGACAAAGAAATTCTCGCTTTACTTAATATCCCCGCTTCAATGC
TTCCGGAAGTTAAACCTAACTCTACCATTTACGGTAAAACCAAAGATTACCACTTTTATGGTAGCGAAGTTCCCATCTCT
GGAATGGCTGGCGATCAGCAAGCGGCCTTATTTGGTCAATTAGCTTTCGAACCCGGAATGGTCAAAAATACCTACGGGAC
TGGTGCATTTACCGTAATGAACACAGGTGAAAAACCGCAATTATCTGATAATAATTTATTAACCACCATTGGTTACGGTA
TTAATGGCAAAGTCTATTACGCACTAGAAGGTAGTATTTTTGTGGCTGGTTCGGCAATTCAATGGCTTCGTGATGGAATG
AAACTGTTTAAAAAGGCTTCTGAATCAGAGGCAGCCGCTGTTGCTTCTCAAAATGATAATGAAGTCTATGTTGTACCAGC
TTTTACCGGACTGGGTGCTCCGTACTGGGATCCTAATGCTCGAGGTTCAGTTTTTGGAATCACTCGTGGAACTACCCGTG
AAGATTTCATTAAAGCTACCCTTCAATCCCTTGCATACCAATCTCGTGACGTTATTGACACTATGAAAAAGGATTCAGGA
ATTGATATTCCTTCCATTCGTGTCGATGGTGGCGCTTCTAATAATGATTACTTGATGCAATTCCAATCTGATATTTTAGG
AATTGAAATTGATCGTGCTAGTGATCTCGAAACCACCGCTCTAGGCGCCGCATTTCTTGCGGGATTAGCAGTTGGATTCT
GGAAAGACCTTGAAGATTTAAAGAAAGAATATAAGCCAGGGAAGGTATTCAAACCAAAGATGTCAACTGATGAGCGTGAA
GACTTATACACAGGTTGGCAAGAAGCGGTTGCAGCAACTCGTCAATTTAAACATCGACCACGACAATCAAAATAA

Upstream 100 bases:

>100_bases
TTTTGTTTTTTAATTATTAAAAACGATTTATAATTAACAACGAGATCTTTAGAAAGCGCTTTCTTGAATCTTAACAATTT
AAAAAAACGGAGGTACGGAC

Downstream 100 bases:

>100_bases
CTTAGGAGGCTTATTTCATGTCATTCTCATTAATTAGTAGAAAACAAGATATCGAAAGATTGAAAAATGAAACGCTCGAC
TTACTTACCATCGGTGGTGG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MADKYILAIDEGTTSTRTIIFDHAGNKMADAQREFPQYFPKPGWVEHNANEIWNAVLSTIANAFIESGIKPNQISGIGIT
NQRETTVIWDKKTGLPIYNAVVWQSRQTAEIAEQLVKDGYGDMIHQKTGLVTDAYFSATKIRWILDHVDGAQARAERGEL
LFGTIDTWLMWKLTDGDVHVTDYSNASRTMLYNIHKLEWDKEILALLNIPASMLPEVKPNSTIYGKTKDYHFYGSEVPIS
GMAGDQQAALFGQLAFEPGMVKNTYGTGAFTVMNTGEKPQLSDNNLLTTIGYGINGKVYYALEGSIFVAGSAIQWLRDGM
KLFKKASESEAAAVASQNDNEVYVVPAFTGLGAPYWDPNARGSVFGITRGTTREDFIKATLQSLAYQSRDVIDTMKKDSG
IDIPSIRVDGGASNNDYLMQFQSDILGIEIDRASDLETTALGAAFLAGLAVGFWKDLEDLKKEYKPGKVFKPKMSTDERE
DLYTGWQEAVAATRQFKHRPRQSK

Sequences:

>Translated_504_residues
MADKYILAIDEGTTSTRTIIFDHAGNKMADAQREFPQYFPKPGWVEHNANEIWNAVLSTIANAFIESGIKPNQISGIGIT
NQRETTVIWDKKTGLPIYNAVVWQSRQTAEIAEQLVKDGYGDMIHQKTGLVTDAYFSATKIRWILDHVDGAQARAERGEL
LFGTIDTWLMWKLTDGDVHVTDYSNASRTMLYNIHKLEWDKEILALLNIPASMLPEVKPNSTIYGKTKDYHFYGSEVPIS
GMAGDQQAALFGQLAFEPGMVKNTYGTGAFTVMNTGEKPQLSDNNLLTTIGYGINGKVYYALEGSIFVAGSAIQWLRDGM
KLFKKASESEAAAVASQNDNEVYVVPAFTGLGAPYWDPNARGSVFGITRGTTREDFIKATLQSLAYQSRDVIDTMKKDSG
IDIPSIRVDGGASNNDYLMQFQSDILGIEIDRASDLETTALGAAFLAGLAVGFWKDLEDLKKEYKPGKVFKPKMSTDERE
DLYTGWQEAVAATRQFKHRPRQSK
>Mature_503_residues
ADKYILAIDEGTTSTRTIIFDHAGNKMADAQREFPQYFPKPGWVEHNANEIWNAVLSTIANAFIESGIKPNQISGIGITN
QRETTVIWDKKTGLPIYNAVVWQSRQTAEIAEQLVKDGYGDMIHQKTGLVTDAYFSATKIRWILDHVDGAQARAERGELL
FGTIDTWLMWKLTDGDVHVTDYSNASRTMLYNIHKLEWDKEILALLNIPASMLPEVKPNSTIYGKTKDYHFYGSEVPISG
MAGDQQAALFGQLAFEPGMVKNTYGTGAFTVMNTGEKPQLSDNNLLTTIGYGINGKVYYALEGSIFVAGSAIQWLRDGMK
LFKKASESEAAAVASQNDNEVYVVPAFTGLGAPYWDPNARGSVFGITRGTTREDFIKATLQSLAYQSRDVIDTMKKDSGI
DIPSIRVDGGASNNDYLMQFQSDILGIEIDRASDLETTALGAAFLAGLAVGFWKDLEDLKKEYKPGKVFKPKMSTDERED
LYTGWQEAVAATRQFKHRPRQSK

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=500, Percent_Identity=44.4, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI4504007, Length=500, Percent_Identity=44.4, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI42794763, Length=504, Percent_Identity=44.047619047619, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI41393575, Length=496, Percent_Identity=43.75, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI88196792, Length=502, Percent_Identity=35.4581673306773, Blast_Score=330, Evalue=2e-90,
Organism=Escherichia coli, GI1790361, Length=487, Percent_Identity=59.1375770020534, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI1790005, Length=529, Percent_Identity=22.4952741020794, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787789, Length=476, Percent_Identity=23.7394957983193, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789987, Length=467, Percent_Identity=22.2698072805139, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789138, Length=509, Percent_Identity=20.8251473477407, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789168, Length=499, Percent_Identity=21.4428857715431, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17535599, Length=436, Percent_Identity=39.6788990825688, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI71986140, Length=525, Percent_Identity=24.5714285714286, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI71986147, Length=303, Percent_Identity=30.3630363036304, Blast_Score=134, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6321755, Length=545, Percent_Identity=33.0275229357798, Blast_Score=298, Evalue=1e-81,
Organism=Drosophila melanogaster, GI17864214, Length=504, Percent_Identity=40.8730158730159, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24654573, Length=447, Percent_Identity=40.9395973154362, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24655555, Length=493, Percent_Identity=36.7139959432049, Blast_Score=340, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24655560, Length=493, Percent_Identity=36.7139959432049, Blast_Score=340, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24655570, Length=493, Percent_Identity=36.7139959432049, Blast_Score=340, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24655574, Length=493, Percent_Identity=36.7139959432049, Blast_Score=340, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24655565, Length=493, Percent_Identity=36.7139959432049, Blast_Score=340, Evalue=2e-93,
Organism=Drosophila melanogaster, GI19922058, Length=520, Percent_Identity=30.9615384615385, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24652871, Length=487, Percent_Identity=31.6221765913758, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI24656367, Length=489, Percent_Identity=28.8343558282209, Blast_Score=227, Evalue=2e-59,
Organism=Drosophila melanogaster, GI21357867, Length=510, Percent_Identity=28.0392156862745, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24656372, Length=336, Percent_Identity=31.25, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI45552909, Length=291, Percent_Identity=27.8350515463918, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_PEDPA (Q03DS8)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_805086.1
- ProteinModelPortal:   Q03DS8
- SMR:   Q03DS8
- STRING:   Q03DS8
- GeneID:   4417900
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1623
- NMPDR:   fig|278197.10.peg.1429
- eggNOG:   COG0554
- HOGENOM:   HBG511469
- OMA:   KILPNVR
- PhylomeDB:   Q03DS8
- ProtClustDB:   PRK00047
- BioCyc:   PPEN278197:PEPE_1623-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55950; Mature: 55818

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 13-13 BINDING 17-17 BINDING 83-83 BINDING 135-135 BINDING 245-245 BINDING 267-267 BINDING 310-310

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKYILAIDEGTTSTRTIIFDHAGNKMADAQREFPQYFPKPGWVEHNANEIWNAVLSTI
CCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
ANAFIESGIKPNQISGIGITNQRETTVIWDKKTGLPIYNAVVWQSRQTAEIAEQLVKDGY
HHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHEEECCCCHHHHHHHHHHCCC
GDMIHQKTGLVTDAYFSATKIRWILDHVDGAQARAERGELLFGTIDTWLMWKLTDGDVHV
HHHHHHHCCCEEHHHHHHHHHEEEEHHCCCHHHHHHCCCEEEEEECEEEEEEEECCCEEE
TDYSNASRTMLYNIHKLEWDKEILALLNIPASMLPEVKPNSTIYGKTKDYHFYGSEVPIS
EECCCCCCEEEEEEEHHCHHHHHHHHHCCCHHHCCCCCCCCEEECCCCCEEEECCCCCCC
GMAGDQQAALFGQLAFEPGMVKNTYGTGAFTVMNTGEKPQLSDNNLLTTIGYGINGKVYY
CCCCCCHHHHHHHHHCCCCCEECCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCEEEE
ALEGSIFVAGSAIQWLRDGMKLFKKASESEAAAVASQNDNEVYVVPAFTGLGAPYWDPNA
EEECCEEEECHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEEEEHHCCCCCCCCCCC
RGSVFGITRGTTREDFIKATLQSLAYQSRDVIDTMKKDSGIDIPSIRVDGGASNNDYLMQ
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEE
FQSDILGIEIDRASDLETTALGAAFLAGLAVGFWKDLEDLKKEYKPGKVFKPKMSTDERE
EECCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHH
DLYTGWQEAVAATRQFKHRPRQSK
HHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ADKYILAIDEGTTSTRTIIFDHAGNKMADAQREFPQYFPKPGWVEHNANEIWNAVLSTI
CCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
ANAFIESGIKPNQISGIGITNQRETTVIWDKKTGLPIYNAVVWQSRQTAEIAEQLVKDGY
HHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHEEECCCCHHHHHHHHHHCCC
GDMIHQKTGLVTDAYFSATKIRWILDHVDGAQARAERGELLFGTIDTWLMWKLTDGDVHV
HHHHHHHCCCEEHHHHHHHHHEEEEHHCCCHHHHHHCCCEEEEEECEEEEEEEECCCEEE
TDYSNASRTMLYNIHKLEWDKEILALLNIPASMLPEVKPNSTIYGKTKDYHFYGSEVPIS
EECCCCCCEEEEEEEHHCHHHHHHHHHCCCHHHCCCCCCCCEEECCCCCEEEECCCCCCC
GMAGDQQAALFGQLAFEPGMVKNTYGTGAFTVMNTGEKPQLSDNNLLTTIGYGINGKVYY
CCCCCCHHHHHHHHHCCCCCEECCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCEEEE
ALEGSIFVAGSAIQWLRDGMKLFKKASESEAAAVASQNDNEVYVVPAFTGLGAPYWDPNA
EEECCEEEECHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEEEEHHCCCCCCCCCCC
RGSVFGITRGTTREDFIKATLQSLAYQSRDVIDTMKKDSGIDIPSIRVDGGASNNDYLMQ
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEE
FQSDILGIEIDRASDLETTALGAAFLAGLAVGFWKDLEDLKKEYKPGKVFKPKMSTDERE
EECCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHH
DLYTGWQEAVAATRQFKHRPRQSK
HHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA