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The map label for this gene is oppA [H]

Identifier: 116493312

GI number: 116493312

Start: 1551386

End: 1553026

Strand: Reverse

Name: oppA [H]

Synonym: PEPE_1584

Alternate gene names: 116493312

Gene position: 1553026-1551386 (Counterclockwise)

Preceding gene: 116493314

Following gene: 116493311

Centisome position: 84.75

GC content: 35.83

Gene sequence:

>1641_bases
ATGAAAAATAAAAAGTGGATCGGGTTTCTTTCGGTTATTTTAGTATCATTGGTGGTATTGGCGGGTTGTGGAGCTAAAAA
AGGTGGCTATGCTGCTGAACAAACCATGAATTTAGCAGCTGATGCTCCCTTGGACACGCTGGATGTATCTAAAGCCAATG
GTTATGGACAAACTGGAAATGTATTTGAAAGTCTATATCGCCTAGGGAAAAATGGTAAGACAACCCCAGGTTTGGCCAGC
GAGGCTAAAGTTTCTAAAGATGGATTGACGTGGACTTTCAAGATTCGCAAAAATGCTAAATTTAGTGATGGTAGTAAAAT
TACAGCTCATGACTTTGTCTATTCATGGCAAAGAACGATTAATCCTAAGACTAAAGCTTTGTATGCTTACTTATTTGAAG
GGATTAAAAATGCCGACCAAATTAATGCAGGTAAGATGGATCCCTCTAAGCTAGGGGTCGAGGCTAAAGATGATCAAACT
TTCGTTGTACATTTGGATCAACCTATTCAATATTTTAAAGTTTTAATGAGTTATCCACTATTTTCACCAATTAGCCAAAA
GGCTGTTGAAAAATATGGTGATAAATATGGAACAAAGGCGCAATATACGGTTTATTCAGGGCCTTTTAAAGTAAAAGGCT
GGACAGGAACGAACGATACTTGGAGTTTCGTTAAAAATCCACAATATTGGGATAAAAAAGTTGTTAAATTGGATCAAATT
AAATTTAGAGTGGTGGAAAACCCACAAACTAAGTTGAATTTATACCAATCCGGGAAGCTAGATTATACACAGCTTTCCAG
TGAACAAGTTAAAAACTATAAAAATAATAAAGATTACACCACCTTTCCATATTCCATTACCACTTTCTTACAATATAATT
TTAATGATGCAGATAAAACCAAACGTAAAGCCTTTAACAATGTAAATATTCGTAAGGCTATCGCCTATTCAATCAATCGG
AAGCAGTTGATTGAAAAAATCATTGGGGATGCTTCAACACCAAGTAAGGGCCTCGTTCCCACGGATTTAGCTAATAATCC
AAAAACGGGAAAGGACTTTGCTGATGACGCATATTTAGACGGTACCATCGAGTACAATCAAAAACAGGCCAAGCAGTACT
GGGAAAAAGGGCTTCAAGAAATTGGAGTAAAGAATTTGAAACTTAATTTAACTTCAGATAATGAGGGGAACAGCAAAAAT
TATGCTGAATTTTATGCACAGTCCTTGGAAAAGACATTACCTGGATTACAAATTCACATTGTTAACGTGCCATCGCAAGT
AGCATATTCGAGATCTCAAAGTGGAGAATTTGATATTTTAGTTGGACATTGGGGAGCTGACTTTAATGATCCAATTTCAC
ATCTTCAAATTTTACAAAAAAATAGTGCATATAATTATGGCAAATGGGACAACACGGAATATAATAGCCTTGTGAAAAAA
GCCCAAAATGAAGATGCTAATAATGCTGAGAAACGCTGGCAAGATATGATTAAGGCAGAACAAATTGTGATGAAAGAACA
AGCTGTTTCACCACTTTATCAAACTGTATATTCATACTTGAAAAATCCTAAAGTAAAAGGCGTTATTCACAACACAGCGG
GTACCCAATGGAACTACAAGTATGCTTATATAACAAATTAG

Upstream 100 bases:

>100_bases
GCCGGTTTTTTGTTTGTTAAGGACGTGATGATATGGAATCTGAATGTAGCGATTGTAGGGTGTTTCCAAGTTGATGTGAT
TAAAATTAAAGGAGTAGAAA

Downstream 100 bases:

>100_bases
TAACAAAAATGTTTTATTTTAAGGAGAAAGTCCCAAATGAGGATTTTCTTCTTTTTATATTGGGCAAGGGGGCGTAATAT
AGGTGGCAAATACTAATAAG

Product: ABC-type oligopeptide transport system, periplasmic component

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MKNKKWIGFLSVILVSLVVLAGCGAKKGGYAAEQTMNLAADAPLDTLDVSKANGYGQTGNVFESLYRLGKNGKTTPGLAS
EAKVSKDGLTWTFKIRKNAKFSDGSKITAHDFVYSWQRTINPKTKALYAYLFEGIKNADQINAGKMDPSKLGVEAKDDQT
FVVHLDQPIQYFKVLMSYPLFSPISQKAVEKYGDKYGTKAQYTVYSGPFKVKGWTGTNDTWSFVKNPQYWDKKVVKLDQI
KFRVVENPQTKLNLYQSGKLDYTQLSSEQVKNYKNNKDYTTFPYSITTFLQYNFNDADKTKRKAFNNVNIRKAIAYSINR
KQLIEKIIGDASTPSKGLVPTDLANNPKTGKDFADDAYLDGTIEYNQKQAKQYWEKGLQEIGVKNLKLNLTSDNEGNSKN
YAEFYAQSLEKTLPGLQIHIVNVPSQVAYSRSQSGEFDILVGHWGADFNDPISHLQILQKNSAYNYGKWDNTEYNSLVKK
AQNEDANNAEKRWQDMIKAEQIVMKEQAVSPLYQTVYSYLKNPKVKGVIHNTAGTQWNYKYAYITN

Sequences:

>Translated_546_residues
MKNKKWIGFLSVILVSLVVLAGCGAKKGGYAAEQTMNLAADAPLDTLDVSKANGYGQTGNVFESLYRLGKNGKTTPGLAS
EAKVSKDGLTWTFKIRKNAKFSDGSKITAHDFVYSWQRTINPKTKALYAYLFEGIKNADQINAGKMDPSKLGVEAKDDQT
FVVHLDQPIQYFKVLMSYPLFSPISQKAVEKYGDKYGTKAQYTVYSGPFKVKGWTGTNDTWSFVKNPQYWDKKVVKLDQI
KFRVVENPQTKLNLYQSGKLDYTQLSSEQVKNYKNNKDYTTFPYSITTFLQYNFNDADKTKRKAFNNVNIRKAIAYSINR
KQLIEKIIGDASTPSKGLVPTDLANNPKTGKDFADDAYLDGTIEYNQKQAKQYWEKGLQEIGVKNLKLNLTSDNEGNSKN
YAEFYAQSLEKTLPGLQIHIVNVPSQVAYSRSQSGEFDILVGHWGADFNDPISHLQILQKNSAYNYGKWDNTEYNSLVKK
AQNEDANNAEKRWQDMIKAEQIVMKEQAVSPLYQTVYSYLKNPKVKGVIHNTAGTQWNYKYAYITN
>Mature_546_residues
MKNKKWIGFLSVILVSLVVLAGCGAKKGGYAAEQTMNLAADAPLDTLDVSKANGYGQTGNVFESLYRLGKNGKTTPGLAS
EAKVSKDGLTWTFKIRKNAKFSDGSKITAHDFVYSWQRTINPKTKALYAYLFEGIKNADQINAGKMDPSKLGVEAKDDQT
FVVHLDQPIQYFKVLMSYPLFSPISQKAVEKYGDKYGTKAQYTVYSGPFKVKGWTGTNDTWSFVKNPQYWDKKVVKLDQI
KFRVVENPQTKLNLYQSGKLDYTQLSSEQVKNYKNNKDYTTFPYSITTFLQYNFNDADKTKRKAFNNVNIRKAIAYSINR
KQLIEKIIGDASTPSKGLVPTDLANNPKTGKDFADDAYLDGTIEYNQKQAKQYWEKGLQEIGVKNLKLNLTSDNEGNSKN
YAEFYAQSLEKTLPGLQIHIVNVPSQVAYSRSQSGEFDILVGHWGADFNDPISHLQILQKNSAYNYGKWDNTEYNSLVKK
AQNEDANNAEKRWQDMIKAEQIVMKEQAVSPLYQTVYSYLKNPKVKGVIHNTAGTQWNYKYAYITN

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=533, Percent_Identity=30.0187617260788, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1789397, Length=544, Percent_Identity=27.3897058823529, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87081878, Length=531, Percent_Identity=27.8719397363465, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1787762, Length=465, Percent_Identity=24.7311827956989, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1787052, Length=344, Percent_Identity=27.0348837209302, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1789966, Length=565, Percent_Identity=23.1858407079646, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61778; Mature: 61778

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKKWIGFLSVILVSLVVLAGCGAKKGGYAAEQTMNLAADAPLDTLDVSKANGYGQTGN
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCCCH
VFESLYRLGKNGKTTPGLASEAKVSKDGLTWTFKIRKNAKFSDGSKITAHDFVYSWQRTI
HHHHHHHHCCCCCCCCCCCCCCEECCCCCEEEEEEECCCCCCCCCEEEHHHHHHHHHHHC
NPKTKALYAYLFEGIKNADQINAGKMDPSKLGVEAKDDQTFVVHLDQPIQYFKVLMSYPL
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCEEEEEECCHHHHHHHHHHCCC
FSPISQKAVEKYGDKYGTKAQYTVYSGPFKVKGWTGTNDTWSFVKNPQYWDKKVVKLDQI
CCHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCHHHCCCCCHHHHHHHEEEEE
KFRVVENPQTKLNLYQSGKLDYTQLSSEQVKNYKNNKDYTTFPYSITTFLQYNFNDADKT
EEEEECCCCCEEEEEECCCCCHHHCCHHHHHHCCCCCCCEECCEEEEEEEEECCCCHHHH
KRKAFNNVNIRKAIAYSINRKQLIEKIIGDASTPSKGLVPTDLANNPKTGKDFADDAYLD
HHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEC
GTIEYNQKQAKQYWEKGLQEIGVKNLKLNLTSDNEGNSKNYAEFYAQSLEKTLPGLQIHI
CEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEE
VNVPSQVAYSRSQSGEFDILVGHWGADFNDPISHLQILQKNSAYNYGKWDNTEYNSLVKK
EECCHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
AQNEDANNAEKRWQDMIKAEQIVMKEQAVSPLYQTVYSYLKNPKVKGVIHNTAGTQWNYK
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEE
YAYITN
EEEEEC
>Mature Secondary Structure
MKNKKWIGFLSVILVSLVVLAGCGAKKGGYAAEQTMNLAADAPLDTLDVSKANGYGQTGN
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCCCH
VFESLYRLGKNGKTTPGLASEAKVSKDGLTWTFKIRKNAKFSDGSKITAHDFVYSWQRTI
HHHHHHHHCCCCCCCCCCCCCCEECCCCCEEEEEEECCCCCCCCCEEEHHHHHHHHHHHC
NPKTKALYAYLFEGIKNADQINAGKMDPSKLGVEAKDDQTFVVHLDQPIQYFKVLMSYPL
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCEEEEEECCHHHHHHHHHHCCC
FSPISQKAVEKYGDKYGTKAQYTVYSGPFKVKGWTGTNDTWSFVKNPQYWDKKVVKLDQI
CCHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCHHHCCCCCHHHHHHHEEEEE
KFRVVENPQTKLNLYQSGKLDYTQLSSEQVKNYKNNKDYTTFPYSITTFLQYNFNDADKT
EEEEECCCCCEEEEEECCCCCHHHCCHHHHHHCCCCCCCEECCEEEEEEEEECCCCHHHH
KRKAFNNVNIRKAIAYSINRKQLIEKIIGDASTPSKGLVPTDLANNPKTGKDFADDAYLD
HHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEC
GTIEYNQKQAKQYWEKGLQEIGVKNLKLNLTSDNEGNSKNYAEFYAQSLEKTLPGLQIHI
CEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEE
VNVPSQVAYSRSQSGEFDILVGHWGADFNDPISHLQILQKNSAYNYGKWDNTEYNSLVKK
EECCHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
AQNEDANNAEKRWQDMIKAEQIVMKEQAVSPLYQTVYSYLKNPKVKGVIHNTAGTQWNYK
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEE
YAYITN
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]