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The map label for this gene is pyrG [H]

Identifier: 116493299

GI number: 116493299

Start: 1540235

End: 1541833

Strand: Reverse

Name: pyrG [H]

Synonym: PEPE_1571

Alternate gene names: 116493299

Gene position: 1541833-1540235 (Counterclockwise)

Preceding gene: 116493300

Following gene: 116493298

Centisome position: 84.14

GC content: 39.4

Gene sequence:

>1599_bases
ATGACCAAATATATTTTTGTTACAGGTGGGGTTGTTTCATCTTTAGGTAAGGGGATTGTTGCTGCATCCCTTGGACGTCT
TTTGAAGAATCGTGGACTTAAGGTTACGATTCAAAAGTTTGATCCATATATTAACGTGGATCCAGGAACGATGAGTCCTT
ACCAACATGGTGAAGTTTTTGTTACTGATGATGGAACAGAAACAGATTTAGATTTAGGCCACTATGAACGTTTTATCGAT
ATCAACTTAAACAAGTATTCTAACGTTACAACTGGAAAGATTTATTCAGAAGTATTACGTAAAGAACGTCATGGCGATTA
TCTTGGAGCAACTGTCCAAGTTATTCCTCATATTACGGACGCAATTAAAGAAAAAATTATGCGCGCTGGTAAAGTGACTG
ATTCTGATATCGTAATCACTGAAATTGGTGGAACGGTTGGGGACATCGAGTCACTTCCTTTCTTGGAAGCTATTCGTCAG
ATGAAGAGTCAAGCCGGATCAGAAAATGTTTTCTACATCCACACGACTTTGGTTCCATACCTAAAAGCTGCAGGGGAAAT
GAAGACAAAGCCAACGCAACACAGTGTTAAAGAACTTCGTGGTTTGGGAATTCAACCTAATATGTTGGTTGTTCGTACAG
AAGAAGATATCACAGATGGTATGCGTTCGAAGATTGCTGCATTCTGTGATGTTGAGCCAGAAGCTGTAATTGAATCTCAA
GATGTTGCAAGTTTATACGAAATTCCATTGAACTTAAAAGCACAAAAGATGGACGACATTGTGATGGACCACTTTGGTTT
GCAAGCACCACAAGCAGACATGTCTAAGTGGGAAGATCTTGTCGATCACGTTAATAACCGTTTGAACCATACCATTAACA
TTGCATTGGTTGGTAAATACGTTGCCTTACAAGATGCTTATATCTCGGTGAATGAAGCGTTAAAACATGCAGGTTATGTT
GTCGATGCCGACATTAAGATTACCAAAATTGATTCAGAAAAACTTACTAAGGAAAATGTTGCTGAACAACTTGCCGGGCA
AGATGGAATCATTGTTCCTGGTGGCTTTGGTGATCGTGGGATTGAAGGAATGATTCATTCCATTCAATATGCTCGTGAAA
ATGATGTGCCATTCTTAGGAATTTGTTTAGGAATGCAAATGGCCAGCGTTGAATTTGCTCGTAACGTATTGGGTTACCAA
GATGCTAATTCGGCTGAAATGGATCCAGCAACTAAGCACAACATTATTGATTTAATGGCTGATCAAGAAGGTGTTAAAGA
TATGGGTGGAACTCAACGCCTCGGTCTTTACCCATGCAAGCTTAAAGAAGGATCTGTAGCTGCCGCCGCTTATAACAACC
AAGAAATGATTGAAGAACGTCATCGTCATCGTTTTGAATTTAACAATGACTATCGTGCCGAAATGGAAGCTAAGGGTCTT
GTATTCTCAGGAACTTCACCAGACAATCGTTTAGTAGAAGTAATTGAGTTACCAGAAAAGAAATTCTTCGTGGCAGCTCA
ATATCACCCAGAATTTTTATCACGTCCACAACGTCCAGAAGGATTATTTAAGGCATTTATCGCTGCTGCAAATAAATAA

Upstream 100 bases:

>100_bases
GGGGGTATTTTTATTGTTGTATCAGTATTTCAACAGTAAAAACAACTGTTTTGCGTTTCCTAATTTAACCAGTCATAAAT
GTTAGAAAAGGAGAATTTTA

Downstream 100 bases:

>100_bases
GCATAAAAATAGTCGAATTTTTCGGCTATTTTTTTATTTGTTGCGTTCATGAGGTTGTATTTTTGGAACAAATTATTTAT
GATTGAGGTTGACTGTAAAA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MTKYIFVTGGVVSSLGKGIVAASLGRLLKNRGLKVTIQKFDPYINVDPGTMSPYQHGEVFVTDDGTETDLDLGHYERFID
INLNKYSNVTTGKIYSEVLRKERHGDYLGATVQVIPHITDAIKEKIMRAGKVTDSDIVITEIGGTVGDIESLPFLEAIRQ
MKSQAGSENVFYIHTTLVPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVVRTEEDITDGMRSKIAAFCDVEPEAVIESQ
DVASLYEIPLNLKAQKMDDIVMDHFGLQAPQADMSKWEDLVDHVNNRLNHTINIALVGKYVALQDAYISVNEALKHAGYV
VDADIKITKIDSEKLTKENVAEQLAGQDGIIVPGGFGDRGIEGMIHSIQYARENDVPFLGICLGMQMASVEFARNVLGYQ
DANSAEMDPATKHNIIDLMADQEGVKDMGGTQRLGLYPCKLKEGSVAAAAYNNQEMIEERHRHRFEFNNDYRAEMEAKGL
VFSGTSPDNRLVEVIELPEKKFFVAAQYHPEFLSRPQRPEGLFKAFIAAANK

Sequences:

>Translated_532_residues
MTKYIFVTGGVVSSLGKGIVAASLGRLLKNRGLKVTIQKFDPYINVDPGTMSPYQHGEVFVTDDGTETDLDLGHYERFID
INLNKYSNVTTGKIYSEVLRKERHGDYLGATVQVIPHITDAIKEKIMRAGKVTDSDIVITEIGGTVGDIESLPFLEAIRQ
MKSQAGSENVFYIHTTLVPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVVRTEEDITDGMRSKIAAFCDVEPEAVIESQ
DVASLYEIPLNLKAQKMDDIVMDHFGLQAPQADMSKWEDLVDHVNNRLNHTINIALVGKYVALQDAYISVNEALKHAGYV
VDADIKITKIDSEKLTKENVAEQLAGQDGIIVPGGFGDRGIEGMIHSIQYARENDVPFLGICLGMQMASVEFARNVLGYQ
DANSAEMDPATKHNIIDLMADQEGVKDMGGTQRLGLYPCKLKEGSVAAAAYNNQEMIEERHRHRFEFNNDYRAEMEAKGL
VFSGTSPDNRLVEVIELPEKKFFVAAQYHPEFLSRPQRPEGLFKAFIAAANK
>Mature_531_residues
TKYIFVTGGVVSSLGKGIVAASLGRLLKNRGLKVTIQKFDPYINVDPGTMSPYQHGEVFVTDDGTETDLDLGHYERFIDI
NLNKYSNVTTGKIYSEVLRKERHGDYLGATVQVIPHITDAIKEKIMRAGKVTDSDIVITEIGGTVGDIESLPFLEAIRQM
KSQAGSENVFYIHTTLVPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVVRTEEDITDGMRSKIAAFCDVEPEAVIESQD
VASLYEIPLNLKAQKMDDIVMDHFGLQAPQADMSKWEDLVDHVNNRLNHTINIALVGKYVALQDAYISVNEALKHAGYVV
DADIKITKIDSEKLTKENVAEQLAGQDGIIVPGGFGDRGIEGMIHSIQYARENDVPFLGICLGMQMASVEFARNVLGYQD
ANSAEMDPATKHNIIDLMADQEGVKDMGGTQRLGLYPCKLKEGSVAAAAYNNQEMIEERHRHRFEFNNDYRAEMEAKGLV
FSGTSPDNRLVEVIELPEKKFFVAAQYHPEFLSRPQRPEGLFKAFIAAANK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=551, Percent_Identity=48.4573502722323, Blast_Score=520, Evalue=1e-147,
Organism=Homo sapiens, GI28559085, Length=551, Percent_Identity=47.1869328493648, Blast_Score=496, Evalue=1e-140,
Organism=Homo sapiens, GI28559083, Length=551, Percent_Identity=47.1869328493648, Blast_Score=496, Evalue=1e-140,
Organism=Homo sapiens, GI221316689, Length=551, Percent_Identity=47.1869328493648, Blast_Score=496, Evalue=1e-140,
Organism=Escherichia coli, GI1789142, Length=541, Percent_Identity=53.9741219963031, Blast_Score=568, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI25148299, Length=604, Percent_Identity=39.7350993377483, Blast_Score=434, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322563, Length=566, Percent_Identity=43.4628975265018, Blast_Score=468, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6319432, Length=559, Percent_Identity=43.8282647584973, Blast_Score=466, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24664469, Length=552, Percent_Identity=46.3768115942029, Blast_Score=490, Evalue=1e-139,
Organism=Drosophila melanogaster, GI21357815, Length=497, Percent_Identity=44.2655935613682, Blast_Score=414, Evalue=1e-116,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59004; Mature: 58873

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYIFVTGGVVSSLGKGIVAASLGRLLKNRGLKVTIQKFDPYINVDPGTMSPYQHGEVF
CCEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTDDGTETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKERHGDYLGATVQVIPHITD
EECCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHCHHHHHHHHHHH
AIKEKIMRAGKVTDSDIVITEIGGTVGDIESLPFLEAIRQMKSQAGSENVFYIHTTLVPY
HHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHH
LKAAGEMKTKPTQHSVKELRGLGIQPNMLVVRTEEDITDGMRSKIAAFCDVEPEAVIESQ
HHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCHHHHHCCH
DVASLYEIPLNLKAQKMDDIVMDHFGLQAPQADMSKWEDLVDHVNNRLNHTINIALVGKY
HHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEE
VALQDAYISVNEALKHAGYVVDADIKITKIDSEKLTKENVAEQLAGQDGIIVPGGFGDRG
HHHHHHHHHHHHHHHHCCEEEECCEEEEECCHHHHHHHHHHHHHCCCCCEEECCCCCCCC
IEGMIHSIQYARENDVPFLGICLGMQMASVEFARNVLGYQDANSAEMDPATKHNIIDLMA
HHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHC
DQEGVKDMGGTQRLGLYPCKLKEGSVAAAAYNNQEMIEERHRHRFEFNNDYRAEMEAKGL
CCCCHHHCCCCCCCCEEEEECCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEECCE
VFSGTSPDNRLVEVIELPEKKFFVAAQYHPEFLSRPQRPEGLFKAFIAAANK
EEECCCCCHHHHHHHCCCCCEEEEEEECCHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TKYIFVTGGVVSSLGKGIVAASLGRLLKNRGLKVTIQKFDPYINVDPGTMSPYQHGEVF
CEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTDDGTETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKERHGDYLGATVQVIPHITD
EECCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHCHHHHHHHHHHH
AIKEKIMRAGKVTDSDIVITEIGGTVGDIESLPFLEAIRQMKSQAGSENVFYIHTTLVPY
HHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHH
LKAAGEMKTKPTQHSVKELRGLGIQPNMLVVRTEEDITDGMRSKIAAFCDVEPEAVIESQ
HHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCHHHHHCCH
DVASLYEIPLNLKAQKMDDIVMDHFGLQAPQADMSKWEDLVDHVNNRLNHTINIALVGKY
HHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEE
VALQDAYISVNEALKHAGYVVDADIKITKIDSEKLTKENVAEQLAGQDGIIVPGGFGDRG
HHHHHHHHHHHHHHHHCCEEEECCEEEEECCHHHHHHHHHHHHHCCCCCEEECCCCCCCC
IEGMIHSIQYARENDVPFLGICLGMQMASVEFARNVLGYQDANSAEMDPATKHNIIDLMA
HHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHC
DQEGVKDMGGTQRLGLYPCKLKEGSVAAAAYNNQEMIEERHRHRFEFNNDYRAEMEAKGL
CCCCHHHCCCCCCCCEEEEECCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEECCE
VFSGTSPDNRLVEVIELPEKKFFVAAQYHPEFLSRPQRPEGLFKAFIAAANK
EEECCCCCHHHHHHHCCCCCEEEEEEECCHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]