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The map label for this gene is rho [H]

Identifier: 116493297

GI number: 116493297

Start: 1537502

End: 1538788

Strand: Reverse

Name: rho [H]

Synonym: PEPE_1569

Alternate gene names: 116493297

Gene position: 1538788-1537502 (Counterclockwise)

Preceding gene: 116493298

Following gene: 116493296

Centisome position: 83.98

GC content: 39.78

Gene sequence:

>1287_bases
ATGGATAATTTTTTAACAATGAATGACTTGCAAGAAATGACTCTCAAAGAAATATACGGCCTTGCCCGTGAATATAAGAT
TTCATATTACAGCCAGATGAACAAGAAGGAATTGTCTCTGGCTGTTTTGCGTGCTCAGGCTAAAAAGAAGTCATTTGTTG
AAATGGAAGGCGTTTTAGATATCGTTGGAAATGATGGATATGGTTTTTTACGGCCAATCAACTATGGTTCTTCGCAAGAA
GATATCTATATTTCAGCATCGCAAATCACACGTTTCGGTTTGCGTAATGGGGATTTAGTTGGTGGTGAAGCTCGCCATCC
CAAACCAGGTGAACGGTATTATGGATTAATGCGGGTTACTTCAGTGAATAATAAGGACCCAGAAGAAGCTAAGCAACGTC
CTCATTTTCCAGCTCTAACCCCCATCTACCCCAATCAACAATTAAAATTAGAAACAACTTCTAATATTTTATCAACGCGA
TTAGTTGATTTGTTTTCACCAATTGGATTTGGACAACGTGGTCTGGTAGTAGCACCACCTAAAGCAGGAAAAACCACTTT
ACTAAAGGCTATTGCTAATGGGATTACCACCAACTATCCAGATGTGAAGCTCATCGTTTTACTAATCGATGAGCGACCAG
AAGAAGTAACAGATTTAGAACGCTCGATTAAGGGAGAAGTGGTTTATTCCACCTTTGATCAAGAACCCCGGAATCATACT
CGTGTTTCAGAATTAGTATTACAACGGGCGCTTCGTCTAGTAGAAGATAAACAAGATGTTGTTGTCTTACTAGATTCACT
TACGCGATTAACCCGTGCATATAATTTAGTGACCTCCCCTAGTGGTCGAACACTTTCTGGTGGGGTTGATCCGGCAGCGT
TTTATAAGCCTAAAAAATTCTTTGGCTCTGCTCGAAATATTGAAGAGGGTGGATCACTTACCATCATTGGGACAGCTTTG
GTTGATACAGGAAGCCGGATGGATGATGTGATTTATGAAGAATTCAAAGGTACGGGTAATTCAGAGCTAGTCTTATCACG
GAATTTAGCTGAGCGCAGAGTATTTCCAGCGTTAGATATCCGCCAGTCAGGAACTCGTAAAGAGGAACTAATGGTCTCAA
AAGATATACTTAATCAGCTTTGGGATATTCGCCGGTCGATGCGTGGAGATGCTCTGGAGTATACGGAACAAGTGCTTAAG
TTATTGAAAAACACTAAAAACAACGAAGCGTTTATTGCTAACTTAAAGAATTTAAATTTAGGTCCTAATGGTAGTAAAAA
ATCTTAA

Upstream 100 bases:

>100_bases
GGATACGACAGTTTGGTTAAAAAACTAACTAAGCTCGGAGCGGATGTTTCAATCACGACCGACAATACATTGTAGTCAAA
GATATAGGAGAGTTAAGTCG

Downstream 100 bases:

>100_bases
TCGACCTTGTTTAAGTTGTTTTTCTATGCTATTATTGGGGACGTTGATTTATAATAAATAACTATGACTCAGCAAATGAC
TCATGGCAAAGGAGCGTAAT

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MDNFLTMNDLQEMTLKEIYGLAREYKISYYSQMNKKELSLAVLRAQAKKKSFVEMEGVLDIVGNDGYGFLRPINYGSSQE
DIYISASQITRFGLRNGDLVGGEARHPKPGERYYGLMRVTSVNNKDPEEAKQRPHFPALTPIYPNQQLKLETTSNILSTR
LVDLFSPIGFGQRGLVVAPPKAGKTTLLKAIANGITTNYPDVKLIVLLIDERPEEVTDLERSIKGEVVYSTFDQEPRNHT
RVSELVLQRALRLVEDKQDVVVLLDSLTRLTRAYNLVTSPSGRTLSGGVDPAAFYKPKKFFGSARNIEEGGSLTIIGTAL
VDTGSRMDDVIYEEFKGTGNSELVLSRNLAERRVFPALDIRQSGTRKEELMVSKDILNQLWDIRRSMRGDALEYTEQVLK
LLKNTKNNEAFIANLKNLNLGPNGSKKS

Sequences:

>Translated_428_residues
MDNFLTMNDLQEMTLKEIYGLAREYKISYYSQMNKKELSLAVLRAQAKKKSFVEMEGVLDIVGNDGYGFLRPINYGSSQE
DIYISASQITRFGLRNGDLVGGEARHPKPGERYYGLMRVTSVNNKDPEEAKQRPHFPALTPIYPNQQLKLETTSNILSTR
LVDLFSPIGFGQRGLVVAPPKAGKTTLLKAIANGITTNYPDVKLIVLLIDERPEEVTDLERSIKGEVVYSTFDQEPRNHT
RVSELVLQRALRLVEDKQDVVVLLDSLTRLTRAYNLVTSPSGRTLSGGVDPAAFYKPKKFFGSARNIEEGGSLTIIGTAL
VDTGSRMDDVIYEEFKGTGNSELVLSRNLAERRVFPALDIRQSGTRKEELMVSKDILNQLWDIRRSMRGDALEYTEQVLK
LLKNTKNNEAFIANLKNLNLGPNGSKKS
>Mature_428_residues
MDNFLTMNDLQEMTLKEIYGLAREYKISYYSQMNKKELSLAVLRAQAKKKSFVEMEGVLDIVGNDGYGFLRPINYGSSQE
DIYISASQITRFGLRNGDLVGGEARHPKPGERYYGLMRVTSVNNKDPEEAKQRPHFPALTPIYPNQQLKLETTSNILSTR
LVDLFSPIGFGQRGLVVAPPKAGKTTLLKAIANGITTNYPDVKLIVLLIDERPEEVTDLERSIKGEVVYSTFDQEPRNHT
RVSELVLQRALRLVEDKQDVVVLLDSLTRLTRAYNLVTSPSGRTLSGGVDPAAFYKPKKFFGSARNIEEGGSLTIIGTAL
VDTGSRMDDVIYEEFKGTGNSELVLSRNLAERRVFPALDIRQSGTRKEELMVSKDILNQLWDIRRSMRGDALEYTEQVLK
LLKNTKNNEAFIANLKNLNLGPNGSKKS

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=421, Percent_Identity=49.8812351543943, Blast_Score=417, Evalue=1e-118,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 48004; Mature: 48004

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNFLTMNDLQEMTLKEIYGLAREYKISYYSQMNKKELSLAVLRAQAKKKSFVEMEGVLD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEE
IVGNDGYGFLRPINYGSSQEDIYISASQITRFGLRNGDLVGGEARHPKPGERYYGLMRVT
EECCCCCCEEEECCCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCHHEEEEEEE
SVNNKDPEEAKQRPHFPALTPIYPNQQLKLETTSNILSTRLVDLFSPIGFGQRGLVVAPP
ECCCCCHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEECC
KAGKTTLLKAIANGITTNYPDVKLIVLLIDERPEEVTDLERSIKGEVVYSTFDQEPRNHT
CCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCHHH
RVSELVLQRALRLVEDKQDVVVLLDSLTRLTRAYNLVTSPSGRTLSGGVDPAAFYKPKKF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCEECCCCCHHHHCCCHHH
FGSARNIEEGGSLTIIGTALVDTGSRMDDVIYEEFKGTGNSELVLSRNLAERRVFPALDI
HCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCCCC
RQSGTRKEELMVSKDILNQLWDIRRSMRGDALEYTEQVLKLLKNTKNNEAFIANLKNLNL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
GPNGSKKS
CCCCCCCC
>Mature Secondary Structure
MDNFLTMNDLQEMTLKEIYGLAREYKISYYSQMNKKELSLAVLRAQAKKKSFVEMEGVLD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEE
IVGNDGYGFLRPINYGSSQEDIYISASQITRFGLRNGDLVGGEARHPKPGERYYGLMRVT
EECCCCCCEEEECCCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCHHEEEEEEE
SVNNKDPEEAKQRPHFPALTPIYPNQQLKLETTSNILSTRLVDLFSPIGFGQRGLVVAPP
ECCCCCHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEECC
KAGKTTLLKAIANGITTNYPDVKLIVLLIDERPEEVTDLERSIKGEVVYSTFDQEPRNHT
CCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCHHH
RVSELVLQRALRLVEDKQDVVVLLDSLTRLTRAYNLVTSPSGRTLSGGVDPAAFYKPKKF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCEECCCCCHHHHCCCHHH
FGSARNIEEGGSLTIIGTALVDTGSRMDDVIYEEFKGTGNSELVLSRNLAERRVFPALDI
HCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCCCC
RQSGTRKEELMVSKDILNQLWDIRRSMRGDALEYTEQVLKLLKNTKNNEAFIANLKNLNL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
GPNGSKKS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423140; 9353933; 9384377 [H]