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The map label for this gene is ldh [H]

Identifier: 116493282

GI number: 116493282

Start: 1523371

End: 1524333

Strand: Direct

Name: ldh [H]

Synonym: PEPE_1554

Alternate gene names: 116493282

Gene position: 1523371-1524333 (Clockwise)

Preceding gene: 116493263

Following gene: 116493283

Centisome position: 83.14

GC content: 40.71

Gene sequence:

>963_bases
TTGTCTAAAATTCAAAACCATCAAAAAGTTGTATTAGTCGGCGACGGCGCTGTAGGTTCAAGTTATGCCTTTGCAATGGC
ACAACAAGGAATTGCAGAAGAATTCGTAATCGTTGATGTTGTTAAGGACCGTACTGTTGGTGACGCACTTGATCTTGAAG
ATGCTACTCCATTTACTGCACCTAAGAACATTTACTCAGGTGAATATTCTGACTGTAAAGATGCAGATCTTGTAGTTATC
ACTGCTGGTGCTCCACAAAAACCAGGTGAAACACGTCTTGACCTTGTTAACAAGAACTTAAACATTCTTTCAACAATCGT
TAAGCCAGTTGTTGACTCAGGCTTCGACGGTATCTTCTTAGTTGCTGCTAACCCAGTTGATATCCTTACTTACGCAACAT
GGAAATTCTCAGGATTCCCTAAAGAAAAGGTTATCGGTTCAGGTATTTCTCTTGATTCAGCACGTCTACGTGTTGCTCTT
GGTAAGAAATTCAACGTTAGCCCTGACTCAGTTGATGCATACATCATGGGTGAACATGGTGACAGTGAATTCGCTGCTTA
CTCAACTGCTTCAATCGGTACAAAGCCATTGCTTGATATCGCTAAAGAAGAAGGCGTTTCAACTGACGAATTAGCTGAAA
TCGAAGACAGTGTTCGTAACAAAGCTTACGAAATCATCAACAAGAAGGGTGCTACATTCTACGGTGTTGGTACTGCTTTG
ATGCGTATTTCAAAAGCTATCTTGCGTGACGAAAATGCTGTATTGCCTGTTGGTGCTTACATGGACGGCGAATATGGTTT
GAATGACATCTACATCGGTACACCTGCTGTTATTAACGGCCAAGGTTTGAACCGTGTTATCGAATCACCACTTAGTGATG
ACGAAATGAAGAAGATGACAGATTCAGCTACAACATTGAAAAAAGTTCTTACAGATGGTTTGAAAGCTCTTGAAGAAAAA
TAA

Upstream 100 bases:

>100_bases
GAACTTCATAAAATTTTTACTCAACTTTCTTTTATGAAAATGCTATACTTAGTATTGTTTGATAAATTCAAATATTATAT
GAAAAAAGGGGATTGATCTT

Downstream 100 bases:

>100_bases
GCTTTTCAACATCAACTAATTTCAAATAGTTGAAAAAACGACTCTGAATAAATATTCAGAGTCGTTTTTTTATGCCCAAT
TTACTAATTTCACGCTTCTA

Product: malate dehydrogenase (NAD)

Products: NA

Alternate protein names: L-LDH [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSKIQNHQKVVLVGDGAVGSSYAFAMAQQGIAEEFVIVDVVKDRTVGDALDLEDATPFTAPKNIYSGEYSDCKDADLVVI
TAGAPQKPGETRLDLVNKNLNILSTIVKPVVDSGFDGIFLVAANPVDILTYATWKFSGFPKEKVIGSGISLDSARLRVAL
GKKFNVSPDSVDAYIMGEHGDSEFAAYSTASIGTKPLLDIAKEEGVSTDELAEIEDSVRNKAYEIINKKGATFYGVGTAL
MRISKAILRDENAVLPVGAYMDGEYGLNDIYIGTPAVINGQGLNRVIESPLSDDEMKKMTDSATTLKKVLTDGLKALEEK

Sequences:

>Translated_320_residues
MSKIQNHQKVVLVGDGAVGSSYAFAMAQQGIAEEFVIVDVVKDRTVGDALDLEDATPFTAPKNIYSGEYSDCKDADLVVI
TAGAPQKPGETRLDLVNKNLNILSTIVKPVVDSGFDGIFLVAANPVDILTYATWKFSGFPKEKVIGSGISLDSARLRVAL
GKKFNVSPDSVDAYIMGEHGDSEFAAYSTASIGTKPLLDIAKEEGVSTDELAEIEDSVRNKAYEIINKKGATFYGVGTAL
MRISKAILRDENAVLPVGAYMDGEYGLNDIYIGTPAVINGQGLNRVIESPLSDDEMKKMTDSATTLKKVLTDGLKALEEK
>Mature_319_residues
SKIQNHQKVVLVGDGAVGSSYAFAMAQQGIAEEFVIVDVVKDRTVGDALDLEDATPFTAPKNIYSGEYSDCKDADLVVIT
AGAPQKPGETRLDLVNKNLNILSTIVKPVVDSGFDGIFLVAANPVDILTYATWKFSGFPKEKVIGSGISLDSARLRVALG
KKFNVSPDSVDAYIMGEHGDSEFAAYSTASIGTKPLLDIAKEEGVSTDELAEIEDSVRNKAYEIINKKGATFYGVGTALM
RISKAILRDENAVLPVGAYMDGEYGLNDIYIGTPAVINGQGLNRVIESPLSDDEMKKMTDSATTLKKVLTDGLKALEEK

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI291575128, Length=310, Percent_Identity=36.7741935483871, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI4557032, Length=310, Percent_Identity=36.7741935483871, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI15082234, Length=308, Percent_Identity=34.4155844155844, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI47059044, Length=308, Percent_Identity=36.3636363636364, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI221136809, Length=308, Percent_Identity=36.3636363636364, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI5031857, Length=304, Percent_Identity=35.8552631578947, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI260099723, Length=304, Percent_Identity=35.8552631578947, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI9257228, Length=313, Percent_Identity=37.0607028753994, Blast_Score=208, Evalue=7e-54,
Organism=Homo sapiens, GI4504973, Length=313, Percent_Identity=37.0607028753994, Blast_Score=208, Evalue=7e-54,
Organism=Homo sapiens, GI260099727, Length=220, Percent_Identity=38.6363636363636, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI260099725, Length=226, Percent_Identity=38.4955752212389, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI207028494, Length=303, Percent_Identity=28.3828382838284, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI103472011, Length=311, Percent_Identity=26.6881028938907, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI103472015, Length=188, Percent_Identity=29.7872340425532, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI21735621, Length=329, Percent_Identity=24.9240121580547, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17535107, Length=292, Percent_Identity=39.041095890411, Blast_Score=220, Evalue=6e-58,
Organism=Caenorhabditis elegans, GI17554310, Length=281, Percent_Identity=25.6227758007117, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17136226, Length=312, Percent_Identity=35.5769230769231, Blast_Score=214, Evalue=8e-56,
Organism=Drosophila melanogaster, GI45550422, Length=303, Percent_Identity=28.7128712871287, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24647881, Length=327, Percent_Identity=24.4648318042813, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 34284; Mature: 34153

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIQNHQKVVLVGDGAVGSSYAFAMAQQGIAEEFVIVDVVKDRTVGDALDLEDATPFTA
CCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCC
PKNIYSGEYSDCKDADLVVITAGAPQKPGETRLDLVNKNLNILSTIVKPVVDSGFDGIFL
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCEEE
VAANPVDILTYATWKFSGFPKEKVIGSGISLDSARLRVALGKKFNVSPDSVDAYIMGEHG
EECCCEEEEEEEEEEECCCCHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCC
DSEFAAYSTASIGTKPLLDIAKEEGVSTDELAEIEDSVRNKAYEIINKKGATFYGVGTAL
CCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
MRISKAILRDENAVLPVGAYMDGEYGLNDIYIGTPAVINGQGLNRVIESPLSDDEMKKMT
HHHHHHHHCCCCCEEEEECEECCCCCCCEEEECCCCEECCCCHHHHHHCCCCHHHHHHHH
DSATTLKKVLTDGLKALEEK
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKIQNHQKVVLVGDGAVGSSYAFAMAQQGIAEEFVIVDVVKDRTVGDALDLEDATPFTA
CCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCC
PKNIYSGEYSDCKDADLVVITAGAPQKPGETRLDLVNKNLNILSTIVKPVVDSGFDGIFL
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCEEE
VAANPVDILTYATWKFSGFPKEKVIGSGISLDSARLRVALGKKFNVSPDSVDAYIMGEHG
EECCCEEEEEEEEEEECCCCHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCC
DSEFAAYSTASIGTKPLLDIAKEEGVSTDELAEIEDSVRNKAYEIINKKGATFYGVGTAL
CCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
MRISKAILRDENAVLPVGAYMDGEYGLNDIYIGTPAVINGQGLNRVIESPLSDDEMKKMT
HHHHHHHHCCCCCEEEEECEECCCCCCCEEEECCCCEECCCCHHHHHHCCCCHHHHHHHH
DSATTLKKVLTDGLKALEEK
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7887607 [H]