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The map label for this gene is asnA

Identifier: 116493207

GI number: 116493207

Start: 1442859

End: 1443866

Strand: Direct

Name: asnA

Synonym: PEPE_1469

Alternate gene names: 116493207

Gene position: 1442859-1443866 (Clockwise)

Preceding gene: 116493193

Following gene: 116493225

Centisome position: 78.74

GC content: 38.89

Gene sequence:

>1008_bases
ATGGATTTAATTATTCCTGCATCATACGATCCAAAATTATCAGTACGTCAAACCCAAGAGGCCATTCGCTACATCCGCGA
AACTTTTCAAGATGAATTTGGAAAAGAACTCAACCTCTCACGTCTCTCTGCACCAATGTTCGTTCCTAGTGCCACTGGGT
TAAACGATAATTTAAATGGAGTTGAAACCCCAGTTTCCTTCTCTATGCAAGATATGCCTGAAACCAGCATCGAAATCGTC
CACTCTCTAGCTAAATGGAAAAGAGTTGCTTTGAAACGCTTTGATTTCGAGATGCACAGTGGACTTTATACTAATATGAA
TGCCATTCGTAAAGACGAAGATCTTGATAATATTCATTCCATCTATGTTGATCAATGGGACTGGGAAAAAGTTATCGATC
AAAGTGAGCGTAATCTAACTACCCTTAAAGAAACAGTTCAAACAATTTTTAAAGTAATCAAACACATGGAGCACGAAGTT
TGGTATAAATATCCTGAAGCCGTCTATCATCTTCCTGATCAAATTCATTTTGTCACTACCCAAGAATTAGAGGATCGTTT
TCCGAAATTAACTCCTAAAGAACGCGAGGACGCCATCTGTAAAGAACTGGGTTGCGTTTTCTTAATGCAAATTGGTGGGA
CGCTAAAAAGTGGTGAACGTCATGATGGACGCGCACCAGATTACGATGATTGGCAATTAAACGGCGATATTTTATTTTGG
TATGAACCTCTTCAAAAAGCCCTTGAAATTTCTAGCATGGGAATCAGAGTTAGTGAAGAGTCGATGCTAAAACAACTAAA
AATAGCTAACGCAGAGGATCGAATCCATCTCCCTTATCATCAAATGTTGTTGAATCATCAACTGCCCTACACCATTGGCG
GTGGAATTGGTCAATCACGTCTTTGCATGTTGCTATTAGGTAAAGCCCATGTTGGTGAAGTTCAAGCTAGTGTCTGGCCT
CAATCGATGATTGATCAATGTGAAGAAAATCAAATTCATATTTTATAA

Upstream 100 bases:

>100_bases
GCAACTTGGGTGGTACCGCGGAAAGTCTTTCGTCCCTTGTCATGGATGAAAGGCTTTTTCTTTTATCCTTAAAATCTAAT
TTATTAAAGGAGAATCAATT

Downstream 100 bases:

>100_bases
ACTAAAATAAAAAAGCTATGACTTAATAAGCGACTTATCCGTCGTTTAGAAATTGAGTCATAGCTTTTTGTATTTTTTAA
TCTTGATCCATTGAGTAAAT

Product: asparagine synthetase AsnA

Products: NA

Alternate protein names: Asparagine synthetase A

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MDLIIPASYDPKLSVRQTQEAIRYIRETFQDEFGKELNLSRLSAPMFVPSATGLNDNLNGVETPVSFSMQDMPETSIEIV
HSLAKWKRVALKRFDFEMHSGLYTNMNAIRKDEDLDNIHSIYVDQWDWEKVIDQSERNLTTLKETVQTIFKVIKHMEHEV
WYKYPEAVYHLPDQIHFVTTQELEDRFPKLTPKEREDAICKELGCVFLMQIGGTLKSGERHDGRAPDYDDWQLNGDILFW
YEPLQKALEISSMGIRVSEESMLKQLKIANAEDRIHLPYHQMLLNHQLPYTIGGGIGQSRLCMLLLGKAHVGEVQASVWP
QSMIDQCEENQIHIL

Sequences:

>Translated_335_residues
MDLIIPASYDPKLSVRQTQEAIRYIRETFQDEFGKELNLSRLSAPMFVPSATGLNDNLNGVETPVSFSMQDMPETSIEIV
HSLAKWKRVALKRFDFEMHSGLYTNMNAIRKDEDLDNIHSIYVDQWDWEKVIDQSERNLTTLKETVQTIFKVIKHMEHEV
WYKYPEAVYHLPDQIHFVTTQELEDRFPKLTPKEREDAICKELGCVFLMQIGGTLKSGERHDGRAPDYDDWQLNGDILFW
YEPLQKALEISSMGIRVSEESMLKQLKIANAEDRIHLPYHQMLLNHQLPYTIGGGIGQSRLCMLLLGKAHVGEVQASVWP
QSMIDQCEENQIHIL
>Mature_335_residues
MDLIIPASYDPKLSVRQTQEAIRYIRETFQDEFGKELNLSRLSAPMFVPSATGLNDNLNGVETPVSFSMQDMPETSIEIV
HSLAKWKRVALKRFDFEMHSGLYTNMNAIRKDEDLDNIHSIYVDQWDWEKVIDQSERNLTTLKETVQTIFKVIKHMEHEV
WYKYPEAVYHLPDQIHFVTTQELEDRFPKLTPKEREDAICKELGCVFLMQIGGTLKSGERHDGRAPDYDDWQLNGDILFW
YEPLQKALEISSMGIRVSEESMLKQLKIANAEDRIHLPYHQMLLNHQLPYTIGGGIGQSRLCMLLLGKAHVGEVQASVWP
QSMIDQCEENQIHIL

Specific function: Asparagine biosynthesis. [C]

COG id: COG2502

COG function: function code E; Asparagine synthetase A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily

Homologues:

Organism=Escherichia coli, GI1790183, Length=315, Percent_Identity=48.8888888888889, Blast_Score=302, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASNA_PEDPA (Q03E72)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804942.1
- ProteinModelPortal:   Q03E72
- SMR:   Q03E72
- STRING:   Q03E72
- GeneID:   4417320
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1469
- NMPDR:   fig|278197.10.peg.1293
- eggNOG:   COG2502
- HOGENOM:   HBG288146
- OMA:   LNDNLNG
- PhylomeDB:   Q03E72
- ProtClustDB:   PRK05425
- BioCyc:   PPEN278197:PEPE_1469-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00555
- InterPro:   IPR006195
- InterPro:   IPR004618
- PIRSF:   PIRSF001555
- TIGRFAMs:   TIGR00669

Pfam domain/function: PF03590 AsnA

EC number: =6.3.1.1

Molecular weight: Translated: 38835; Mature: 38835

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLIIPASYDPKLSVRQTQEAIRYIRETFQDEFGKELNLSRLSAPMFVPSATGLNDNLNG
CCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCC
VETPVSFSMQDMPETSIEIVHSLAKWKRVALKRFDFEMHSGLYTNMNAIRKDEDLDNIHS
CCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCHHHHHCCCCHHHHHH
IYVDQWDWEKVIDQSERNLTTLKETVQTIFKVIKHMEHEVWYKYPEAVYHLPDQIHFVTT
HHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEEH
QELEDRFPKLTPKEREDAICKELGCVFLMQIGGTLKSGERHDGRAPDYDDWQLNGDILFW
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCEEEE
YEPLQKALEISSMGIRVSEESMLKQLKIANAEDRIHLPYHQMLLNHQLPYTIGGGIGQSR
EHHHHHHHHHHHCCCEECHHHHHHHHHHCCCCCCEECCHHHHHHCCCCCEEECCCCCHHH
LCMLLLGKAHVGEVQASVWPQSMIDQCEENQIHIL
HHHHHHCCCHHCHHHHHCCCHHHHHHHCCCCEECC
>Mature Secondary Structure
MDLIIPASYDPKLSVRQTQEAIRYIRETFQDEFGKELNLSRLSAPMFVPSATGLNDNLNG
CCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCC
VETPVSFSMQDMPETSIEIVHSLAKWKRVALKRFDFEMHSGLYTNMNAIRKDEDLDNIHS
CCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCHHHHHCCCCHHHHHH
IYVDQWDWEKVIDQSERNLTTLKETVQTIFKVIKHMEHEVWYKYPEAVYHLPDQIHFVTT
HHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEEH
QELEDRFPKLTPKEREDAICKELGCVFLMQIGGTLKSGERHDGRAPDYDDWQLNGDILFW
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCEEEE
YEPLQKALEISSMGIRVSEESMLKQLKIANAEDRIHLPYHQMLLNHQLPYTIGGGIGQSR
EHHHHHHHHHHHCCCEECHHHHHHHHHHCCCCCCEECCHHHHHHCCCCCEEECCCCCHHH
LCMLLLGKAHVGEVQASVWPQSMIDQCEENQIHIL
HHHHHHCCCHHCHHHHHCCCHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA