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The map label for this gene is rpoA

Identifier: 116493137

GI number: 116493137

Start: 1379645

End: 1380589

Strand: Reverse

Name: rpoA

Synonym: PEPE_1392

Alternate gene names: 116493137

Gene position: 1380589-1379645 (Counterclockwise)

Preceding gene: 116493138

Following gene: 116493136

Centisome position: 75.34

GC content: 37.35

Gene sequence:

>945_bases
ATGATCGAATTTGAGAAGCCAAAAATTCATAAAATTGACGAAAATGACAATTATGGCCGATTTGTTATTGAACCCTTAGA
ACGGGGATATGGTACAACGCTTGGTAATTCACTTCGTCGTATCCTATTATCATCATTGCCAGGAGCAGCTATTACTAGCG
TTCAAATTGATAGTGTTTTACATGAATTCTCAACCATTGAAGGTGTTGTAGAAGACGTAACACAAATTATTTTGAATCTT
AAGAAGGTTTCGTTGAAGATTGAATCTGATGAAGATAAAACATTAGAAATCAATGTTGCGGGACCTGCTAATGTAACTGC
TGGCGATATTATGGGAGACGGTGACGTAGTGATTCTTAATCCTGAGTTACCAATCTGTACTGTAGCTGAAGGTACTACAT
TCCATGCAGTACTCACTGCTGACACTGGCCGTGGATATACATCTGCGGACGAAAACAAGGCTCGAAAGGTTGATATGCCA
ATCGGTGTATTACCAATCGATTCTATTTATACCCCAATTGAACGTGTTAATTATCAAGTTGAAAATACTCGTGTTGGTCA
ACGAGACGACTATGATAAACTTACGCTCGATATTTGGACTGATGGTTCTATCACTCCTAGTGAGGCAACAAGTTTATCTG
CTAAAATTTTAACAGAACACCTTGCAATCTTTGTTAACCTTAATGATCAGGCTAAAGAGACTGAAATTATGGTTGAAAAA
GAAGAAACTCACAAGGAAAAAATGCTTGAAATGACAATTGAAGAATTAGATCTTTCGGTCCGTTCATACAATTGCTTAAA
ACGAGCAGGTATTAACAGTGTTCAAGAATTAACTGACCGCACTGATGCTCAAATGATGAAAGTTCGTAATCTTGGACGTA
AGTCATTGGTTGAAATCCAAGAGAAACTTAGTTTATTGGGCTTAGGTTTCCGTTCAGAAGACTAA

Upstream 100 bases:

>100_bases
AGCGCCGTCGTGTATAGAGTTCGGTTCTGTTTGAGGGCAAACTTTATAATGCATTCAAAATCGAAATGCAGACTGCGTTT
TGAAAGGGGTAAAAATTAGA

Downstream 100 bases:

>100_bases
TCATAAGGAGGGTTCCCATAGATGAGTTATCGTAAATTAGGTCGTACAAGCTCCCAACGTAAAGCGTTGCTTCGCGATCT
CACAACAGAATTAATTGTTA

Product: DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MIEFEKPKIHKIDENDNYGRFVIEPLERGYGTTLGNSLRRILLSSLPGAAITSVQIDSVLHEFSTIEGVVEDVTQIILNL
KKVSLKIESDEDKTLEINVAGPANVTAGDIMGDGDVVILNPELPICTVAEGTTFHAVLTADTGRGYTSADENKARKVDMP
IGVLPIDSIYTPIERVNYQVENTRVGQRDDYDKLTLDIWTDGSITPSEATSLSAKILTEHLAIFVNLNDQAKETEIMVEK
EETHKEKMLEMTIEELDLSVRSYNCLKRAGINSVQELTDRTDAQMMKVRNLGRKSLVEIQEKLSLLGLGFRSED

Sequences:

>Translated_314_residues
MIEFEKPKIHKIDENDNYGRFVIEPLERGYGTTLGNSLRRILLSSLPGAAITSVQIDSVLHEFSTIEGVVEDVTQIILNL
KKVSLKIESDEDKTLEINVAGPANVTAGDIMGDGDVVILNPELPICTVAEGTTFHAVLTADTGRGYTSADENKARKVDMP
IGVLPIDSIYTPIERVNYQVENTRVGQRDDYDKLTLDIWTDGSITPSEATSLSAKILTEHLAIFVNLNDQAKETEIMVEK
EETHKEKMLEMTIEELDLSVRSYNCLKRAGINSVQELTDRTDAQMMKVRNLGRKSLVEIQEKLSLLGLGFRSED
>Mature_314_residues
MIEFEKPKIHKIDENDNYGRFVIEPLERGYGTTLGNSLRRILLSSLPGAAITSVQIDSVLHEFSTIEGVVEDVTQIILNL
KKVSLKIESDEDKTLEINVAGPANVTAGDIMGDGDVVILNPELPICTVAEGTTFHAVLTADTGRGYTSADENKARKVDMP
IGVLPIDSIYTPIERVNYQVENTRVGQRDDYDKLTLDIWTDGSITPSEATSLSAKILTEHLAIFVNLNDQAKETEIMVEK
EETHKEKMLEMTIEELDLSVRSYNCLKRAGINSVQELTDRTDAQMMKVRNLGRKSLVEIQEKLSLLGLGFRSED

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=317, Percent_Identity=43.217665615142, Blast_Score=249, Evalue=1e-67,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_PEDPA (Q03EE2)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804872.1
- ProteinModelPortal:   Q03EE2
- STRING:   Q03EE2
- GeneID:   4417810
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1392
- NMPDR:   fig|278197.10.peg.1226
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- PhylomeDB:   Q03EE2
- BioCyc:   PPEN278197:PEPE_1392-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 34910; Mature: 34910

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEFEKPKIHKIDENDNYGRFVIEPLERGYGTTLGNSLRRILLSSLPGAAITSVQIDSVL
CCCCCCCCCEECCCCCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHH
HEFSTIEGVVEDVTQIILNLKKVSLKIESDEDKTLEINVAGPANVTAGDIMGDGDVVILN
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCCCCEECCCCEEEEC
PELPICTVAEGTTFHAVLTADTGRGYTSADENKARKVDMPIGVLPIDSIYTPIERVNYQV
CCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCCEEECCCEEEEEHHHHHCCHHHCCEEE
ENTRVGQRDDYDKLTLDIWTDGSITPSEATSLSAKILTEHLAIFVNLNDQAKETEIMVEK
ECCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHEEEEEEEECCCCCCCCEEEEEC
EETHKEKMLEMTIEELDLSVRSYNCLKRAGINSVQELTDRTDAQMMKVRNLGRKSLVEIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EKLSLLGLGFRSED
HHHHHHCCCCCCCC
>Mature Secondary Structure
MIEFEKPKIHKIDENDNYGRFVIEPLERGYGTTLGNSLRRILLSSLPGAAITSVQIDSVL
CCCCCCCCCEECCCCCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHH
HEFSTIEGVVEDVTQIILNLKKVSLKIESDEDKTLEINVAGPANVTAGDIMGDGDVVILN
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCCCCEECCCCEEEEC
PELPICTVAEGTTFHAVLTADTGRGYTSADENKARKVDMPIGVLPIDSIYTPIERVNYQV
CCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCCEEECCCEEEEEHHHHHCCHHHCCEEE
ENTRVGQRDDYDKLTLDIWTDGSITPSEATSLSAKILTEHLAIFVNLNDQAKETEIMVEK
ECCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHEEEEEEEECCCCCCCCEEEEEC
EETHKEKMLEMTIEELDLSVRSYNCLKRAGINSVQELTDRTDAQMMKVRNLGRKSLVEIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EKLSLLGLGFRSED
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA