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The map label for this gene is truA [H]

Identifier: 116493132

GI number: 116493132

Start: 1375826

End: 1376572

Strand: Reverse

Name: truA [H]

Synonym: PEPE_1387

Alternate gene names: 116493132

Gene position: 1376572-1375826 (Counterclockwise)

Preceding gene: 116493133

Following gene: 116493131

Centisome position: 75.12

GC content: 36.28

Gene sequence:

>747_bases
ATGCGATATAAAATAATATTAGCTTATGACGGGACTAATTTTGCAGGTTTTCAAATGCAACCTAATCAGCGAACTGTGGA
AGGGGTCTTAAAAAGAATAGTTAATAAAATAGCTAAAAATCCAACCCCGGAAATTTCAGTTTATGGATCAGGACGTACAG
ATGCTGGAGTACATGCTTTAGGACAAGTGGTTCATTTTGATTTATCCATTGAAATGTCAGAACGTAGCATGCTACACGCA
TTAAATAGTCAATTACCATTAGATATGCAAGTAAAAAAGGTTGAAATAGTTGATGAAACATTTCATGCGCGTTTTACCAC
ACATGGTAAAAAGTATCAGTATCGAATGGCTTTGGGACAATTCGTAGACCCGTTTAAAAGAAATTATACAGGCCATTGGA
AATTCCCCATTAATATTGGGTTAATCCAAAAAGCGATTAAAGATTTAGAAGGTACTCATGATTTTTCGAGTTTTGTTGCA
TCAGGGAGTACCGTAAAAGATCATGTTCGAACCATTTATGAAGCTAAAGTATGGGAAGATAAAAAACAAAATGAAATCGT
GTTTGAATTTTATGGCAATGGCTTCTTATACAACATGGTTCGAATTATGGTGGCAGTTTTAGTTGAAATAGGTAGTAAAC
GTCGTCCAGCAGATGATATACCACGTCTCTTTGAAGTTAAGGACCGAAATCAGGCCCGGGGGACAGCACCTGCTAGCGGG
CTATATTTAAAAGAAGTAGATTATTAG

Upstream 100 bases:

>100_bases
AGTATCGAGTTTTAAATTATACAAAAAATGACATAATAGGTACGATATTCATGATGGTTTCTGTAGCATTGATTATGGTA
CTCAGAAATTGGAGTTAGTA

Downstream 100 bases:

>100_bases
ATTTTTGATTGACTTTAATAGGGACTGTTAATATAATGGAAGTTGGTATTGTTTACCTCCACAAAATAAGCCCCGGAAAC
TTATTAAAGTTGAGTTAAAC

Product: pseudouridylate synthase

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MRYKIILAYDGTNFAGFQMQPNQRTVEGVLKRIVNKIAKNPTPEISVYGSGRTDAGVHALGQVVHFDLSIEMSERSMLHA
LNSQLPLDMQVKKVEIVDETFHARFTTHGKKYQYRMALGQFVDPFKRNYTGHWKFPINIGLIQKAIKDLEGTHDFSSFVA
SGSTVKDHVRTIYEAKVWEDKKQNEIVFEFYGNGFLYNMVRIMVAVLVEIGSKRRPADDIPRLFEVKDRNQARGTAPASG
LYLKEVDY

Sequences:

>Translated_248_residues
MRYKIILAYDGTNFAGFQMQPNQRTVEGVLKRIVNKIAKNPTPEISVYGSGRTDAGVHALGQVVHFDLSIEMSERSMLHA
LNSQLPLDMQVKKVEIVDETFHARFTTHGKKYQYRMALGQFVDPFKRNYTGHWKFPINIGLIQKAIKDLEGTHDFSSFVA
SGSTVKDHVRTIYEAKVWEDKKQNEIVFEFYGNGFLYNMVRIMVAVLVEIGSKRRPADDIPRLFEVKDRNQARGTAPASG
LYLKEVDY
>Mature_248_residues
MRYKIILAYDGTNFAGFQMQPNQRTVEGVLKRIVNKIAKNPTPEISVYGSGRTDAGVHALGQVVHFDLSIEMSERSMLHA
LNSQLPLDMQVKKVEIVDETFHARFTTHGKKYQYRMALGQFVDPFKRNYTGHWKFPINIGLIQKAIKDLEGTHDFSSFVA
SGSTVKDHVRTIYEAKVWEDKKQNEIVFEFYGNGFLYNMVRIMVAVLVEIGSKRRPADDIPRLFEVKDRNQARGTAPASG
LYLKEVDY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=278, Percent_Identity=32.3741007194245, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI199559785, Length=261, Percent_Identity=28.3524904214559, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1788657, Length=248, Percent_Identity=33.8709677419355, Blast_Score=143, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI71985046, Length=255, Percent_Identity=25.0980392156863, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI116007752, Length=302, Percent_Identity=27.4834437086093, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281362146, Length=259, Percent_Identity=27.027027027027, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648455, Length=259, Percent_Identity=27.027027027027, Blast_Score=71, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28353; Mature: 28353

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYKIILAYDGTNFAGFQMQPNQRTVEGVLKRIVNKIAKNPTPEISVYGSGRTDAGVHAL
CCEEEEEEECCCCEECEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
GQVVHFDLSIEMSERSMLHALNSQLPLDMQVKKVEIVDETFHARFTTHGKKYQYRMALGQ
HHHEEEEEEEEEHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHHEECCCCCEEHHHHHHH
FVDPFKRNYTGHWKFPINIGLIQKAIKDLEGTHDFSSFVASGSTVKDHVRTIYEAKVWED
HHHHHHHCCCCEEEEEEEHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCC
KKQNEIVFEFYGNGFLYNMVRIMVAVLVEIGSKRRPADDIPRLFEVKDRNQARGTAPASG
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHCCCCCCCCCCCCCCCC
LYLKEVDY
EEEEECCC
>Mature Secondary Structure
MRYKIILAYDGTNFAGFQMQPNQRTVEGVLKRIVNKIAKNPTPEISVYGSGRTDAGVHAL
CCEEEEEEECCCCEECEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
GQVVHFDLSIEMSERSMLHALNSQLPLDMQVKKVEIVDETFHARFTTHGKKYQYRMALGQ
HHHEEEEEEEEEHHHHHHHHHHCCCCCCEEEEEEEEHHHHHHHHEECCCCCEEHHHHHHH
FVDPFKRNYTGHWKFPINIGLIQKAIKDLEGTHDFSSFVASGSTVKDHVRTIYEAKVWED
HHHHHHHCCCCEEEEEEEHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCC
KKQNEIVFEFYGNGFLYNMVRIMVAVLVEIGSKRRPADDIPRLFEVKDRNQARGTAPASG
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHCCCCCCCCCCCCCCCC
LYLKEVDY
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA