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The map label for this gene is greA [H]

Identifier: 116493088

GI number: 116493088

Start: 1330203

End: 1330667

Strand: Direct

Name: greA [H]

Synonym: PEPE_1340

Alternate gene names: 116493088

Gene position: 1330203-1330667 (Clockwise)

Preceding gene: 116493087

Following gene: 116493098

Centisome position: 72.59

GC content: 41.72

Gene sequence:

>465_bases
ATGCAACCAAAGTTTGTACCTATGACTCCTAGTGGCTACGCTAAAATTAATCAAGAAATTGATGATTTAAAGCAGACACG
TCCGGCCTTAATCAAGGCCCTTGCCGATGCCCGAGCCTTGGGTGATCTTTCTGAAAATGCCGAATATAGTGCCGCCAAAC
GTGACCTTCGTCGGATGGAAGGCCGACTACGATACTTGCAGCGTCAAATTAGTTATTCCAAAATCATTCAAAACACTAAT
CATTCCAAAATCGATTTGGGTAGCATAGTCAAGATGCGTTTTGTCGATGACCATGACGAAATCACCTACGAGATTGTGGG
AAAACATGAGGCGGACGTGGTTAACAACAAAATCTCTTTCCAATCTCCCCTAGGTTCAGCACTTATGCACCATAAAACCC
ATGATATTGTCCTAGTTACTGCTCCTGATGAACAATATCAGGTTGAAATTCTTGAAATTAATTAA

Upstream 100 bases:

>100_bases
TAGATTCTGTCTGGGTAACCACCTCACATATCCTTGAAAAGCATGAATATATGATTGAACTTACTTTCGAATCATCATCC
ATGTAGAAAGGAGTGATTTT

Downstream 100 bases:

>100_bases
GGTCTTTATTGACGAAACGTATTTTTTAGTGTAAATTAATAGAGTAATTTGCCCGCTGGTCAAATTGGTTAAGACGTCGG
CCTCTCAAGCCGGAGTTACG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA 1 [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MQPKFVPMTPSGYAKINQEIDDLKQTRPALIKALADARALGDLSENAEYSAAKRDLRRMEGRLRYLQRQISYSKIIQNTN
HSKIDLGSIVKMRFVDDHDEITYEIVGKHEADVVNNKISFQSPLGSALMHHKTHDIVLVTAPDEQYQVEILEIN

Sequences:

>Translated_154_residues
MQPKFVPMTPSGYAKINQEIDDLKQTRPALIKALADARALGDLSENAEYSAAKRDLRRMEGRLRYLQRQISYSKIIQNTN
HSKIDLGSIVKMRFVDDHDEITYEIVGKHEADVVNNKISFQSPLGSALMHHKTHDIVLVTAPDEQYQVEILEIN
>Mature_154_residues
MQPKFVPMTPSGYAKINQEIDDLKQTRPALIKALADARALGDLSENAEYSAAKRDLRRMEGRLRYLQRQISYSKIIQNTN
HSKIDLGSIVKMRFVDDHDEITYEIVGKHEADVVNNKISFQSPLGSALMHHKTHDIVLVTAPDEQYQVEILEIN

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=36.1842105263158, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI87082261, Length=140, Percent_Identity=32.8571428571429, Blast_Score=73, Evalue=6e-15,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17560; Mature: 17560

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPKFVPMTPSGYAKINQEIDDLKQTRPALIKALADARALGDLSENAEYSAAKRDLRRME
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
GRLRYLQRQISYSKIIQNTNHSKIDLGSIVKMRFVDDHDEITYEIVGKHEADVVNNKISF
HHHHHHHHHHHHHHHHHCCCCCEEEHHHEEEEEEECCCCCEEEEEECCCCHHHHCCCCCC
QSPLGSALMHHKTHDIVLVTAPDEQYQVEILEIN
CCCHHHHHHHCCCCCEEEEECCCCCEEEEEEEEC
>Mature Secondary Structure
MQPKFVPMTPSGYAKINQEIDDLKQTRPALIKALADARALGDLSENAEYSAAKRDLRRME
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
GRLRYLQRQISYSKIIQNTNHSKIDLGSIVKMRFVDDHDEITYEIVGKHEADVVNNKISF
HHHHHHHHHHHHHHHHHCCCCCEEEHHHEEEEEEECCCCCEEEEEECCCCHHHHCCCCCC
QSPLGSALMHHKTHDIVLVTAPDEQYQVEILEIN
CCCHHHHHHHCCCCCEEEEECCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]