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The map label for this gene is glyA

Identifier: 116493075

GI number: 116493075

Start: 1316265

End: 1317497

Strand: Reverse

Name: glyA

Synonym: PEPE_1327

Alternate gene names: 116493075

Gene position: 1317497-1316265 (Counterclockwise)

Preceding gene: 116493076

Following gene: 116493074

Centisome position: 71.9

GC content: 39.33

Gene sequence:

>1233_bases
ATGAATTATACAAAACAAGATCCAGAATTATGGTCAGCAATTGAACGTGAAGAAAAGCGTCAGCAACATAATATTGAATT
AATTGCTTCGGAAAATATTGTATCTGATAGCGTACGCAAGGCCCAAGGTAGTGTACTTACTAATAAGTATGCCGAAGGTT
ATCCTGGCAAACGTTACTATGGTGGGTGTGAATTTATTGACCAAGTTGAACAATTAGCAATTGATCGGGCAAAAGCCATC
TTTAATGCGGAATATGTAAATGTTCAACCCCATTCTGGTTCCCAAGCGAATGCTGCGGCTTATGCCGCGTTTATTAAACC
TGGGGATAAAATTTTAGGGATGGACCTTAATGCTGGTGGACATTTAACACACGGTGCTAAAGTAAGCTTTAGTGGAACTT
TTTATCAATCAGTGAGTTATGGGGTTGATCCTCAAACTGAAAAATTAGATTACGATGAAATTCGTCGTATTGCTTTAAAA
GAACAGCCACAAATTATTGTTGCGGGAGCTTCGGCCTATAGTCGATTTATTGATTGGAATAAATTCCGCGAAATTGCTGA
TGAAGTCGGGGCTTATCTGATGGTAGATATGGCCCATATTGCTGGTCTGGTAGCAGCTGGTCTGCATCCTTCACCAGTTG
GCATTGCTGATGTGGTTACAACTACGACACATAAGACCCTCCGAGGTCCCCGTGGTGGCATGATTCTTTCGCAGGAAAAA
TATGCCAAGCAACTTAATTTTGCGGTATTTCCTCAAAATCAAGGTGGTCCATTAGAACATGTTATTGCTGCGAAGGCCGT
TGCCTTTGGTGAAGCATTGCAACCAGAATTTAAGACTTATCAAAAACAAGTTTTAAAAAATGCGAAAGCAATGGCTGAAG
TCTTTGAAAATGATACGATTTTGCACGTAGTAACAGGGGGGACCGATAATCATCTGATGACAGTAGATTTAACTGGGACC
CAATTAAATGGTAAGGAAGTACAGAATCTGTTAGATGGCGTCTTCATTACTACTAATAAAGAAGCTATTCCAGAAGAAAA
ATTAAGCCCATTTAAAACCTCAGGAATTCGAATTGGAACGCCAGCTATTACGACACGAGGATTTAAAGAAGACGACTGTC
GTGAAGTTGCTAATTTGATAATTCGAGCAATCAAAAATGCGGATAATGAAACAGTTCTAGAAGAAATTAAGCGAGATGTG
TTCAAACTAACTGAAAAGTACCCTATTGATTAA

Upstream 100 bases:

>100_bases
GGATTTGATAGAATAGGGATTGGCGAGGCATACATGAATCGTCTCCAAAAATCTGCAGGTAACACATACTTTAGTGACAT
CGATTAGGGGGAATTGGTCA

Downstream 100 bases:

>100_bases
ATTAGTCCTAGTTTTTTTATGTTTTTTTAGCTACAATTGATAAGGAATTTGAAAGGAGCGAGTTTAGTCATGGGTAAGTT
TGAGGTTCTCGATCATCCGT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNYTKQDPELWSAIEREEKRQQHNIELIASENIVSDSVRKAQGSVLTNKYAEGYPGKRYYGGCEFIDQVEQLAIDRAKAI
FNAEYVNVQPHSGSQANAAAYAAFIKPGDKILGMDLNAGGHLTHGAKVSFSGTFYQSVSYGVDPQTEKLDYDEIRRIALK
EQPQIIVAGASAYSRFIDWNKFREIADEVGAYLMVDMAHIAGLVAAGLHPSPVGIADVVTTTTHKTLRGPRGGMILSQEK
YAKQLNFAVFPQNQGGPLEHVIAAKAVAFGEALQPEFKTYQKQVLKNAKAMAEVFENDTILHVVTGGTDNHLMTVDLTGT
QLNGKEVQNLLDGVFITTNKEAIPEEKLSPFKTSGIRIGTPAITTRGFKEDDCREVANLIIRAIKNADNETVLEEIKRDV
FKLTEKYPID

Sequences:

>Translated_410_residues
MNYTKQDPELWSAIEREEKRQQHNIELIASENIVSDSVRKAQGSVLTNKYAEGYPGKRYYGGCEFIDQVEQLAIDRAKAI
FNAEYVNVQPHSGSQANAAAYAAFIKPGDKILGMDLNAGGHLTHGAKVSFSGTFYQSVSYGVDPQTEKLDYDEIRRIALK
EQPQIIVAGASAYSRFIDWNKFREIADEVGAYLMVDMAHIAGLVAAGLHPSPVGIADVVTTTTHKTLRGPRGGMILSQEK
YAKQLNFAVFPQNQGGPLEHVIAAKAVAFGEALQPEFKTYQKQVLKNAKAMAEVFENDTILHVVTGGTDNHLMTVDLTGT
QLNGKEVQNLLDGVFITTNKEAIPEEKLSPFKTSGIRIGTPAITTRGFKEDDCREVANLIIRAIKNADNETVLEEIKRDV
FKLTEKYPID
>Mature_410_residues
MNYTKQDPELWSAIEREEKRQQHNIELIASENIVSDSVRKAQGSVLTNKYAEGYPGKRYYGGCEFIDQVEQLAIDRAKAI
FNAEYVNVQPHSGSQANAAAYAAFIKPGDKILGMDLNAGGHLTHGAKVSFSGTFYQSVSYGVDPQTEKLDYDEIRRIALK
EQPQIIVAGASAYSRFIDWNKFREIADEVGAYLMVDMAHIAGLVAAGLHPSPVGIADVVTTTTHKTLRGPRGGMILSQEK
YAKQLNFAVFPQNQGGPLEHVIAAKAVAFGEALQPEFKTYQKQVLKNAKAMAEVFENDTILHVVTGGTDNHLMTVDLTGT
QLNGKEVQNLLDGVFITTNKEAIPEEKLSPFKTSGIRIGTPAITTRGFKEDDCREVANLIIRAIKNADNETVLEEIKRDV
FKLTEKYPID

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=403, Percent_Identity=46.6501240694789, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI261862352, Length=403, Percent_Identity=46.6501240694789, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI261862350, Length=403, Percent_Identity=46.6501240694789, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI261862348, Length=403, Percent_Identity=46.6501240694789, Blast_Score=367, Evalue=1e-102,
Organism=Homo sapiens, GI261862346, Length=403, Percent_Identity=46.1538461538462, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI22547186, Length=403, Percent_Identity=46.4019851116625, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI22547189, Length=388, Percent_Identity=45.1030927835052, Blast_Score=304, Evalue=1e-82,
Organism=Escherichia coli, GI1788902, Length=412, Percent_Identity=58.9805825242718, Blast_Score=469, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI25144729, Length=421, Percent_Identity=46.3182897862233, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI25144732, Length=421, Percent_Identity=46.3182897862233, Blast_Score=359, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6319739, Length=412, Percent_Identity=46.6019417475728, Blast_Score=368, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6323087, Length=423, Percent_Identity=46.0992907801418, Blast_Score=355, Evalue=7e-99,
Organism=Drosophila melanogaster, GI24640005, Length=449, Percent_Identity=45.2115812917595, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI221329721, Length=449, Percent_Identity=45.2115812917595, Blast_Score=378, Evalue=1e-105,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_PEDPA (Q03EK4)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804810.1
- ProteinModelPortal:   Q03EK4
- SMR:   Q03EK4
- STRING:   Q03EK4
- GeneID:   4417014
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1327
- NMPDR:   fig|278197.10.peg.1165
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   RPRMLIG
- PhylomeDB:   Q03EK4
- BioCyc:   PPEN278197:PEPE_1327-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45224; Mature: 45224

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYTKQDPELWSAIEREEKRQQHNIELIASENIVSDSVRKAQGSVLTNKYAEGYPGKRYY
CCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCHHHHHHHCCCCCCCCC
GGCEFIDQVEQLAIDRAKAIFNAEYVNVQPHSGSQANAAAYAAFIKPGDKILGMDLNAGG
CCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHEEEEECCCCEEEEEEECCCC
HLTHGAKVSFSGTFYQSVSYGVDPQTEKLDYDEIRRIALKEQPQIIVAGASAYSRFIDWN
CCCCCCEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHH
KFREIADEVGAYLMVDMAHIAGLVAAGLHPSPVGIADVVTTTTHKTLRGPRGGMILSQEK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECHHH
YAKQLNFAVFPQNQGGPLEHVIAAKAVAFGEALQPEFKTYQKQVLKNAKAMAEVFENDTI
HHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCE
LHVVTGGTDNHLMTVDLTGTQLNGKEVQNLLDGVFITTNKEAIPEEKLSPFKTSGIRIGT
EEEEECCCCCEEEEEEECCCCCCHHHHHHHHCCEEEECCCCCCCHHHCCCCCCCCEEECC
PAITTRGFKEDDCREVANLIIRAIKNADNETVLEEIKRDVFKLTEKYPID
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNYTKQDPELWSAIEREEKRQQHNIELIASENIVSDSVRKAQGSVLTNKYAEGYPGKRYY
CCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCHHHHHHHCCCCCCCCC
GGCEFIDQVEQLAIDRAKAIFNAEYVNVQPHSGSQANAAAYAAFIKPGDKILGMDLNAGG
CCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHEEEEECCCCEEEEEEECCCC
HLTHGAKVSFSGTFYQSVSYGVDPQTEKLDYDEIRRIALKEQPQIIVAGASAYSRFIDWN
CCCCCCEEEECCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHH
KFREIADEVGAYLMVDMAHIAGLVAAGLHPSPVGIADVVTTTTHKTLRGPRGGMILSQEK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECHHH
YAKQLNFAVFPQNQGGPLEHVIAAKAVAFGEALQPEFKTYQKQVLKNAKAMAEVFENDTI
HHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCE
LHVVTGGTDNHLMTVDLTGTQLNGKEVQNLLDGVFITTNKEAIPEEKLSPFKTSGIRIGT
EEEEECCCCCEEEEEEECCCCCCHHHHHHHHCCEEEECCCCCCCHHHCCCCCCCCEEECC
PAITTRGFKEDDCREVANLIIRAIKNADNETVLEEIKRDVFKLTEKYPID
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA