Click here to switch to the map view.

The map label for this gene is mraW

Identifier: 116492952

GI number: 116492952

Start: 1192398

End: 1193342

Strand: Reverse

Name: mraW

Synonym: PEPE_1191

Alternate gene names: 116492952

Gene position: 1193342-1192398 (Counterclockwise)

Preceding gene: 116492953

Following gene: 116492951

Centisome position: 65.12

GC content: 37.14

Gene sequence:

>945_bases
ATGACGGAATTTAAACATACAACGGTTCTCTTAGAAGAGACCGTTAACGGCTTAGATGTAAAAGAAGATGGTATTTATGT
AGACTGCACCCTAGGTGGCGGTGGACATAGTAGACTAATTTTGTCTAAGTTGGGAGAAAATGGTCACCTCTATTCATTTG
ACCAAGATCAAACGGCAATTGATTACAATCAAAAAAATTTAGCAGAATATGTTGCTAAGGGACAAGTTACTTTTGTAAAA
TCAAATTTTCGTAATTTAAAGAATGAATTAAACCACTTGGGGATTGATGGAGTTGATGGCGTTTTATACGATTTAGGCGT
TTCATCACCCCAATTTGACGACGCAGGACGAGGATTTAGTTATCGATTTGATGCTCCTTTAGACATGCGTATGGATCAAA
CTCAAAAATTGAATGCTAGAGAAGTTGTTAATGAATGGAGCTATAACGATTTAGTACGAATTTTCTTTAGATATGGAGAA
GAAAAATATTCTAAGCAAGTTGCTCGAAAAATTGAAAGTACTCGCGAAGAAAAGCCCATTGAAACTACTGGTGAACTAGC
CGATTTAATTAAAAGCGCAATCCCGGCGGCAGCAAGGCGAACGGGTGGACATCCTGCTAAAAAGATTTTCCAGGCCATTC
GAATTGCGGTTAATGATGAATTGGGTGCTTTAGAAGAATCATTGGAACAAGCCCTACAAATTTTGAATCCAGCAGGACGT
ATTTCGGTTATTACTTTCCAATCATTAGAAGATCGGTTAGTAAAGAGAATGTTTAAAGAACAAACTGAGATTGGTGATTT
ACCGGCTGGACTGCCAATTATCCCAGATGATATGAAACCAAAATACAAGTTGATCAATAGGAAGCCGATTATTCCTTCTG
AAGATGAACTTGAAGAAAACAGAAGATCACATAGCGCAAAACTAAGAATTATCGAAAAGTTATAG

Upstream 100 bases:

>100_bases
AGATATGGAATAAAGCCCGCTGGGAAGAAACTATTGAAGAAACTGAAGAAAACTTCGACGATATCGCCGAAAATTTAATT
GACTTTGGATTGTGATAAAT

Downstream 100 bases:

>100_bases
AAGGGATAGACGCCAATGGCTCAAAATAATTATGCTGGACGTAGAACAAAATTAATTAACGACACAGTAATTACGGGCAT
TAGTGAAAAAGAATTAAAAA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MTEFKHTTVLLEETVNGLDVKEDGIYVDCTLGGGGHSRLILSKLGENGHLYSFDQDQTAIDYNQKNLAEYVAKGQVTFVK
SNFRNLKNELNHLGIDGVDGVLYDLGVSSPQFDDAGRGFSYRFDAPLDMRMDQTQKLNAREVVNEWSYNDLVRIFFRYGE
EKYSKQVARKIESTREEKPIETTGELADLIKSAIPAAARRTGGHPAKKIFQAIRIAVNDELGALEESLEQALQILNPAGR
ISVITFQSLEDRLVKRMFKEQTEIGDLPAGLPIIPDDMKPKYKLINRKPIIPSEDELEENRRSHSAKLRIIEKL

Sequences:

>Translated_314_residues
MTEFKHTTVLLEETVNGLDVKEDGIYVDCTLGGGGHSRLILSKLGENGHLYSFDQDQTAIDYNQKNLAEYVAKGQVTFVK
SNFRNLKNELNHLGIDGVDGVLYDLGVSSPQFDDAGRGFSYRFDAPLDMRMDQTQKLNAREVVNEWSYNDLVRIFFRYGE
EKYSKQVARKIESTREEKPIETTGELADLIKSAIPAAARRTGGHPAKKIFQAIRIAVNDELGALEESLEQALQILNPAGR
ISVITFQSLEDRLVKRMFKEQTEIGDLPAGLPIIPDDMKPKYKLINRKPIIPSEDELEENRRSHSAKLRIIEKL
>Mature_313_residues
TEFKHTTVLLEETVNGLDVKEDGIYVDCTLGGGGHSRLILSKLGENGHLYSFDQDQTAIDYNQKNLAEYVAKGQVTFVKS
NFRNLKNELNHLGIDGVDGVLYDLGVSSPQFDDAGRGFSYRFDAPLDMRMDQTQKLNAREVVNEWSYNDLVRIFFRYGEE
KYSKQVARKIESTREEKPIETTGELADLIKSAIPAAARRTGGHPAKKIFQAIRIAVNDELGALEESLEQALQILNPAGRI
SVITFQSLEDRLVKRMFKEQTEIGDLPAGLPIIPDDMKPKYKLINRKPIIPSEDELEENRRSHSAKLRIIEKL

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=339, Percent_Identity=35.1032448377581, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI165377202, Length=190, Percent_Identity=37.8947368421053, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1786270, Length=319, Percent_Identity=43.5736677115987, Blast_Score=258, Evalue=3e-70,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=37.4622356495468, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=37.4622356495468, Blast_Score=176, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_PEDPA (Q03EX7)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804687.1
- ProteinModelPortal:   Q03EX7
- STRING:   Q03EX7
- GeneID:   4417924
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1191
- NMPDR:   fig|278197.10.peg.1049
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   Q03EX7
- ProtClustDB:   PRK00050
- BioCyc:   PPEN278197:PEPE_1191-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35485; Mature: 35354

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: BINDING 54-54 BINDING 83-83 BINDING 104-104 BINDING 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEFKHTTVLLEETVNGLDVKEDGIYVDCTLGGGGHSRLILSKLGENGHLYSFDQDQTAI
CCCCHHHHEEHHHHCCCCCEECCCEEEEEEECCCCCHHHHHHHCCCCCCEEECCCCCCEE
DYNQKNLAEYVAKGQVTFVKSNFRNLKNELNHLGIDGVDGVLYDLGVSSPQFDDAGRGFS
CCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCE
YRFDAPLDMRMDQTQKLNAREVVNEWSYNDLVRIFFRYGEEKYSKQVARKIESTREEKPI
EEECCCCCCCCCHHHCCCHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCC
ETTGELADLIKSAIPAAARRTGGHPAKKIFQAIRIAVNDELGALEESLEQALQILNPAGR
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
ISVITFQSLEDRLVKRMFKEQTEIGDLPAGLPIIPDDMKPKYKLINRKPIIPSEDELEEN
EEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHH
RRSHSAKLRIIEKL
HHHHHHHHHHHHCC
>Mature Secondary Structure 
TEFKHTTVLLEETVNGLDVKEDGIYVDCTLGGGGHSRLILSKLGENGHLYSFDQDQTAI
CCCHHHHEEHHHHCCCCCEECCCEEEEEEECCCCCHHHHHHHCCCCCCEEECCCCCCEE
DYNQKNLAEYVAKGQVTFVKSNFRNLKNELNHLGIDGVDGVLYDLGVSSPQFDDAGRGFS
CCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCE
YRFDAPLDMRMDQTQKLNAREVVNEWSYNDLVRIFFRYGEEKYSKQVARKIESTREEKPI
EEECCCCCCCCCHHHCCCHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCC
ETTGELADLIKSAIPAAARRTGGHPAKKIFQAIRIAVNDELGALEESLEQALQILNPAGR
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
ISVITFQSLEDRLVKRMFKEQTEIGDLPAGLPIIPDDMKPKYKLINRKPIIPSEDELEEN
EEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHH
RRSHSAKLRIIEKL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA