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The map label for this gene is cspA [H]

Identifier: 116492939

GI number: 116492939

Start: 1176990

End: 1177199

Strand: Reverse

Name: cspA [H]

Synonym: PEPE_1178

Alternate gene names: 116492939

Gene position: 1177199-1176990 (Counterclockwise)

Preceding gene: 116492940

Following gene: 116492938

Centisome position: 64.24

GC content: 35.71

Gene sequence:

>210_bases
TTGGAAACTGGAACAGTAGAAAGCTTTGATAAAGAAAAAGGGTATGGATTTATTGAAACAGCTAGTGGAGATAAAGTATT
CGCGCATTACACGGTCATTCAATCTGAAGATTATAAAACTTTAGAAGTCGGTGAAAAGGTTAAATTAATGCTTGCAGATG
GACCTAAAGGGTTAATCGCGGTTAAAGTTTTTCGTGAGGAGGTTGAATAA

Upstream 100 bases:

>100_bases
TAAGAATGATTAGGCGAGTAACCTAATCATTTTTAATTTACAGAAGTGTTTTCCACCATTATGATGGTAGTATGACAAAC
ATAAAAAAGGAGCTTAAATT

Downstream 100 bases:

>100_bases
TAATGAGTTTTGAAGAAAAAGTAGTAGAGTCTATTTCAAGATATAAAGGCGAAGTTATTGATGTTTATCAACAAACGGTG
AAATTACCAGACGGCGAATT

Product: cold-shock DNA-binding protein family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 69; Mature: 69

Protein sequence:

>69_residues
METGTVESFDKEKGYGFIETASGDKVFAHYTVIQSEDYKTLEVGEKVKLMLADGPKGLIAVKVFREEVE

Sequences:

>Translated_69_residues
METGTVESFDKEKGYGFIETASGDKVFAHYTVIQSEDYKTLEVGEKVKLMLADGPKGLIAVKVFREEVE
>Mature_69_residues
METGTVESFDKEKGYGFIETASGDKVFAHYTVIQSEDYKTLEVGEKVKLMLADGPKGLIAVKVFREEVE

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=70, Percent_Identity=44.2857142857143, Blast_Score=62, Evalue=9e-12,
Organism=Escherichia coli, GI1787834, Length=62, Percent_Identity=48.3870967741936, Blast_Score=60, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7690; Mature: 7690

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METGTVESFDKEKGYGFIETASGDKVFAHYTVIQSEDYKTLEVGEKVKLMLADGPKGLIA
CCCCCCCCCCCCCCCEEEEECCCCEEEEEEEEEECCCCEEEECCCEEEEEEECCCCCEEE
VKVFREEVE
EEEEEECCC
>Mature Secondary Structure
METGTVESFDKEKGYGFIETASGDKVFAHYTVIQSEDYKTLEVGEKVKLMLADGPKGLIA
CCCCCCCCCCCCCCCEEEEECCCCEEEEEEEEEECCCCEEEECCCEEEEEEECCCCCEEE
VKVFREEVE
EEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]