Click here to switch to the map view.

The map label for this gene is fruK [H]

Identifier: 116492926

GI number: 116492926

Start: 1164828

End: 1165739

Strand: Reverse

Name: fruK [H]

Synonym: PEPE_1165

Alternate gene names: 116492926

Gene position: 1165739-1164828 (Counterclockwise)

Preceding gene: 116492927

Following gene: 116492925

Centisome position: 63.62

GC content: 35.2

Gene sequence:

>912_bases
ATGATTTACACAGTTACACTTAACCCATCGATTGATTATGTAATCAGTCTGGATGAAATGAAAGTTGGCTTGGTCAATCG
CTTGAAGACATCAAGTAAATTTCCGGGTGGAAAAGGGATTAATGTTTCAAGAATTTTAAATGAATTAAATGTGTCTAACA
CAGCGTTAGGTTTTCTAGGAGGATTTACTGGGAAATTTGTGAATGATGCATTAACAAGATTGAATGTGCAAACCGATTTT
GTTGAGATTAAAGAAGATACTAGGATTAATGTAAAAATTCATGCTCAACAAGAAACAGAATTAAATGCTCTCGGGCCTAA
TATTGATGATGCAGAATTAGCAAGTTTTATCGACAAATTTAATCAAATTGGCAATGGAGATGTTGTTGTATTTTCAGGAA
GTATCCCAGCCAATTTACCTCAGAACCTTTATGATCAAATAATTAAAAAAATTAAGGCAAATGGGGCGCAACTTGCAGTT
GATACTACGGGACAGGGTCTATTAGATACCTTAGAAAATAATCCAATCGTTATCAAGCCAAATAACCATGAACTTGGAGA
ATTATTTCATACGGAAGTAACCACCGATGCAGATATTGAGAAGTATGCTAAAAAGTTAATTGAAATGGGCGCTCAAAATG
TCATGATTTCAATGGCTGGTGAAGGTGGGATGCTAGTTTCCAAGGATCGTGTGCTTAGAAGTTTTGCTCCAAAAGGCAAA
GTCTTAAATTCTGTAGGAGCTGGGGACTCAATGTTGGCTGGATTTGTTGGAGAATTCAATCAGTCACATGATTTAGAAAA
AGCCTTTAAAAAAGGTTTAGCGTGTGGAAGTGCCACAGCATTTTCACTGGATATTGCAACTAATAAAAAAATTGACGAGA
TTTTTAACGAAATTGATGTCTTTGAAAAGTAA

Upstream 100 bases:

>100_bases
TTGTACGTTTTGCAGAATTGCAAGATGCAACAATTATTGCCGACCATTGTCCAGAATGGCTCAAAGCAGAGGAAAATATA
TATTATATGGAGGTTCAAAA

Downstream 100 bases:

>100_bases
GGGGTAAAATTATGGAGATTAAAGATTTACTCAAGAAAAACTTAATGATTATGGATCTAAAAGCAGATTCAAAATCGGAT
GCAATTAACGAAATGATTGA

Product: tagatose-6-phosphate kinase

Products: NA

Alternate protein names: Fructose 1-phosphate kinase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MIYTVTLNPSIDYVISLDEMKVGLVNRLKTSSKFPGGKGINVSRILNELNVSNTALGFLGGFTGKFVNDALTRLNVQTDF
VEIKEDTRINVKIHAQQETELNALGPNIDDAELASFIDKFNQIGNGDVVVFSGSIPANLPQNLYDQIIKKIKANGAQLAV
DTTGQGLLDTLENNPIVIKPNNHELGELFHTEVTTDADIEKYAKKLIEMGAQNVMISMAGEGGMLVSKDRVLRSFAPKGK
VLNSVGAGDSMLAGFVGEFNQSHDLEKAFKKGLACGSATAFSLDIATNKKIDEIFNEIDVFEK

Sequences:

>Translated_303_residues
MIYTVTLNPSIDYVISLDEMKVGLVNRLKTSSKFPGGKGINVSRILNELNVSNTALGFLGGFTGKFVNDALTRLNVQTDF
VEIKEDTRINVKIHAQQETELNALGPNIDDAELASFIDKFNQIGNGDVVVFSGSIPANLPQNLYDQIIKKIKANGAQLAV
DTTGQGLLDTLENNPIVIKPNNHELGELFHTEVTTDADIEKYAKKLIEMGAQNVMISMAGEGGMLVSKDRVLRSFAPKGK
VLNSVGAGDSMLAGFVGEFNQSHDLEKAFKKGLACGSATAFSLDIATNKKIDEIFNEIDVFEK
>Mature_303_residues
MIYTVTLNPSIDYVISLDEMKVGLVNRLKTSSKFPGGKGINVSRILNELNVSNTALGFLGGFTGKFVNDALTRLNVQTDF
VEIKEDTRINVKIHAQQETELNALGPNIDDAELASFIDKFNQIGNGDVVVFSGSIPANLPQNLYDQIIKKIKANGAQLAV
DTTGQGLLDTLENNPIVIKPNNHELGELFHTEVTTDADIEKYAKKLIEMGAQNVMISMAGEGGMLVSKDRVLRSFAPKGK
VLNSVGAGDSMLAGFVGEFNQSHDLEKAFKKGLACGSATAFSLDIATNKKIDEIFNEIDVFEK

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=285, Percent_Identity=30.5263157894737, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1788493, Length=258, Percent_Identity=29.8449612403101, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022463
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.56 [H]

Molecular weight: Translated: 32856; Mature: 32856

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYTVTLNPSIDYVISLDEMKVGLVNRLKTSSKFPGGKGINVSRILNELNVSNTALGFLG
CEEEEEECCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHH
GFTGKFVNDALTRLNVQTDFVEIKEDTRINVKIHAQQETELNALGPNIDDAELASFIDKF
CCHHHHHHHHHHHCCCCHHEEEECCCCEEEEEEEECCCCCHHHCCCCCCHHHHHHHHHHH
NQIGNGDVVVFSGSIPANLPQNLYDQIIKKIKANGAQLAVDTTGQGLLDTLENNPIVIKP
HHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCEEEEC
NNHELGELFHTEVTTDADIEKYAKKLIEMGAQNVMISMAGEGGMLVSKDRVLRSFAPKGK
CCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEHHHHHHHHCCCCH
VLNSVGAGDSMLAGFVGEFNQSHDLEKAFKKGLACGSATAFSLDIATNKKIDEIFNEIDV
HHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHH
FEK
CCC
>Mature Secondary Structure
MIYTVTLNPSIDYVISLDEMKVGLVNRLKTSSKFPGGKGINVSRILNELNVSNTALGFLG
CEEEEEECCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHH
GFTGKFVNDALTRLNVQTDFVEIKEDTRINVKIHAQQETELNALGPNIDDAELASFIDKF
CCHHHHHHHHHHHCCCCHHEEEECCCCEEEEEEEECCCCCHHHCCCCCCHHHHHHHHHHH
NQIGNGDVVVFSGSIPANLPQNLYDQIIKKIKANGAQLAVDTTGQGLLDTLENNPIVIKP
HHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCEEEEC
NNHELGELFHTEVTTDADIEKYAKKLIEMGAQNVMISMAGEGGMLVSKDRVLRSFAPKGK
CCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEHHHHHHHHCCCCH
VLNSVGAGDSMLAGFVGEFNQSHDLEKAFKKGLACGSATAFSLDIATNKKIDEIFNEIDV
HHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHH
FEK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]