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The map label for this gene is trmD

Identifier: 116492616

GI number: 116492616

Start: 856237

End: 856974

Strand: Direct

Name: trmD

Synonym: PEPE_0849

Alternate gene names: 116492616

Gene position: 856237-856974 (Clockwise)

Preceding gene: 116492615

Following gene: 116492617

Centisome position: 46.73

GC content: 39.7

Gene sequence:

>738_bases
ATGAAAATTGATGTTTTAACCTTATTTCCTAAAATGGTAGAAGTTCCATTGCATGAATCCATTGTTGGTAAAGCGATTGA
AGCCGGTAAGTTAAATGTTGACGTCACTGATTTTCGACAATATTCCACTAATAAACATGGAAATGTCGATGATTATCCAT
ATGGAGGCGGAGCAGGGATGCTGCTAACACCTCAACCTATTTTTGATGCAATGGAGCATGTTAAGAGCCAAAATAACCAA
TCCACCGGGCGGGTTATTTTAGTTGATCCTGGCGGCAAGCAATTTAACCAAACGATGGCTGAAGATTTTGCTAAAGAAGA
ACATCTAACATTTATTTGTGGACATTACGAAGGCTATGATGAGCGGATTAAAACACTTGTCGATGATGAAGTATCCTTGG
GAGACTTTGTAATTACTGGTGGTGAGTTAGCTGCGTTAACCATGATTGATGCTACAGTACGTTTATTACCTGACATATTG
GGAAATAAAGACTCTGCAGTCGATGATTCATTTTCAACGGGTCTACTTGAATTTCCACAATATACGCGACCTGAAGATTT
TCGAGGGCTGAAGGTTCCATCAATTTTGTTGAGCGGAAATCATGGAAAAATCGATGAATGGCGACGGACAGAGTCTTTAA
AAAAGACACTTGCAAGGCGCCCCGATCTGTTAGAAACAGCTCAGTTAACACCGGAAGATAAACAAATCTTACGAGAACTT
CAAACGCCCGATTTATAA

Upstream 100 bases:

>100_bases
ATCAAAACGATTTGTTGCTTCCATATTTAAAAGATGTGATTTTAAATGTTGATTTGGAGCAAGGAGCAGTTCAAGTTAGT
GTTCCAGAGGGGTTAGATTA

Downstream 100 bases:

>100_bases
AATCGTTATAGGCTAAGTCCAAAATAATTTTGCACTTAATAATAGAATATGTTAGGATATTAATTGGTTTGAAAACCAAC
AATAACAATGTTCCGCTGGC

Product: tRNA (Guanine37-N1) methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKIDVLTLFPKMVEVPLHESIVGKAIEAGKLNVDVTDFRQYSTNKHGNVDDYPYGGGAGMLLTPQPIFDAMEHVKSQNNQ
STGRVILVDPGGKQFNQTMAEDFAKEEHLTFICGHYEGYDERIKTLVDDEVSLGDFVITGGELAALTMIDATVRLLPDIL
GNKDSAVDDSFSTGLLEFPQYTRPEDFRGLKVPSILLSGNHGKIDEWRRTESLKKTLARRPDLLETAQLTPEDKQILREL
QTPDL

Sequences:

>Translated_245_residues
MKIDVLTLFPKMVEVPLHESIVGKAIEAGKLNVDVTDFRQYSTNKHGNVDDYPYGGGAGMLLTPQPIFDAMEHVKSQNNQ
STGRVILVDPGGKQFNQTMAEDFAKEEHLTFICGHYEGYDERIKTLVDDEVSLGDFVITGGELAALTMIDATVRLLPDIL
GNKDSAVDDSFSTGLLEFPQYTRPEDFRGLKVPSILLSGNHGKIDEWRRTESLKKTLARRPDLLETAQLTPEDKQILREL
QTPDL
>Mature_245_residues
MKIDVLTLFPKMVEVPLHESIVGKAIEAGKLNVDVTDFRQYSTNKHGNVDDYPYGGGAGMLLTPQPIFDAMEHVKSQNNQ
STGRVILVDPGGKQFNQTMAEDFAKEEHLTFICGHYEGYDERIKTLVDDEVSLGDFVITGGELAALTMIDATVRLLPDIL
GNKDSAVDDSFSTGLLEFPQYTRPEDFRGLKVPSILLSGNHGKIDEWRRTESLKKTLARRPDLLETAQLTPEDKQILREL
QTPDL

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=243, Percent_Identity=42.798353909465, Blast_Score=212, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_PEDPA (Q03FW3)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804351.1
- ProteinModelPortal:   Q03FW3
- SMR:   Q03FW3
- STRING:   Q03FW3
- GeneID:   4417966
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0849
- NMPDR:   fig|278197.10.peg.753
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   Q03FW3
- BioCyc:   PPEN278197:PEPE_0849-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27319; Mature: 27319

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: BINDING 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDVLTLFPKMVEVPLHESIVGKAIEAGKLNVDVTDFRQYSTNKHGNVDDYPYGGGAGM
CEEEHEEHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCE
LLTPQPIFDAMEHVKSQNNQSTGRVILVDPGGKQFNQTMAEDFAKEEHLTFICGHYEGYD
EECCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCCHH
ERIKTLVDDEVSLGDFVITGGELAALTMIDATVRLLPDILGNKDSAVDDSFSTGLLEFPQ
HHHHHHHCCCCCCCCEEEECCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
YTRPEDFRGLKVPSILLSGNHGKIDEWRRTESLKKTLARRPDLLETAQLTPEDKQILREL
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHH
QTPDL
CCCCC
>Mature Secondary Structure
MKIDVLTLFPKMVEVPLHESIVGKAIEAGKLNVDVTDFRQYSTNKHGNVDDYPYGGGAGM
CEEEHEEHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCE
LLTPQPIFDAMEHVKSQNNQSTGRVILVDPGGKQFNQTMAEDFAKEEHLTFICGHYEGYD
EECCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEECCCCHH
ERIKTLVDDEVSLGDFVITGGELAALTMIDATVRLLPDILGNKDSAVDDSFSTGLLEFPQ
HHHHHHHCCCCCCCCEEEECCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
YTRPEDFRGLKVPSILLSGNHGKIDEWRRTESLKKTLARRPDLLETAQLTPEDKQILREL
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHH
QTPDL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA