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The map label for this gene is asnB [H]

Identifier: 116492428

GI number: 116492428

Start: 697166

End: 699046

Strand: Direct

Name: asnB [H]

Synonym: PEPE_0659

Alternate gene names: 116492428

Gene position: 697166-699046 (Clockwise)

Preceding gene: 116492426

Following gene: 116492429

Centisome position: 38.05

GC content: 36.04

Gene sequence:

>1881_bases
ATGTGCGGATTTGCTGGTTACATTAATCAAGCTAATGTAAATAAAGACACAATCAAGTCAATGGCCGATCGAATTAAACA
CCGAGGACCAGACGATGAAGCATTTTTCCAAGATGAAAATGCGTCAATGGGATTCCGTCGCCTATCAATTATTGATTTGG
CACACGGACGTCAACCCATGATGAATAGTGCTGAAACTAAAGTATTAACTTTCAATGGTGAAATTTATAACTACAAACAA
GTTCGTGAAGAACTAAAAGAATTAGGATATGAATTTAGAACGGATGTTGATTCAGAAGTCCTAATCCATGGTTATGACGC
ATGGGGACCAGAATTACTTAAAAAACTTCGTGGGATGTTTGCCTTTGTAATTTATGATAAAGAAAAACAAGAAGTATTTG
GTGCTCGTGATCATTTTGGAATCAAGCCTCTTTACTACTACGATGATGATAAAACGTTCCTTTGGGGTTCAGAAATCAAA
TCATTCCTAGAACATCCAAACTTTGTGAAGGAATTCAATGAAGAACTATTACCAATCCACTTAAGTTTTGAATTTATTCC
TTCTAAAGAAACGATGTTTAAGAATGTTTACAAGCTTCTTCCAGGAACTTACTTCATTCATAAAAATGGTAAAACAGAAA
CCCATACTTACTTTAAATTTAACTACGACCATATCGACGAATCACAAACTATCGAAGAAGATTCAAAGAAAATTCGAAAG
ATTGTTAGTGACTCTGTAGATGCACATATGATTGCAGACGTTGAAGTTGGTAGTTTCTTATCTAGTGGAATTGATTCAAG
TTATGTTTTAGCAGAAGCTGCTAAACTTAAACCTATCCAATCTTTCTCACTTGGATTTAGAAATTCTAAGTATAGTGAAT
TAAGTTACTCAACTGAATTTGCTAAGGAAATCAATCAAAAGAATACACCACTCTACATGGATGGTGATGATTACTTTGAT
ATTTTGCCAACCATCATGTACTACATGGATGAACCACTATCAAATCCATCAGCAATGCAATTATTCTATCTTTCCAAAGG
TACTCGTAAGAATGTCAAAGTGGCTCTTTCAGGAGAAGGTGCCGATGAATTCTTTGGTGGATACAATACTTATCTAGAAG
CTTTCACATTTGAACGTTACCAAAAGATGGTTCCTAGCTTTATTCGAAAAGGGTTGGCTAAGGCAGTTTCAGGTCTTCCT
CGTTTCCATGGACGTCGTTTCTTGATGCGTGGGGCTCAGCCACTTAGTGAACGCTATTACCGCGTAAATTACGTATTTAA
TCAACAAGAACGTGATCAAATTTTACGTAATCCAGCTTTGAATGTTGATTCAGGAGCTTATACACAACATATCTTTGACG
AAGTGAAGGGCAAAGATGAGATGACTCAAATGCAATACTTTGACATCAATACTTGGCTTCCATTTGATATCTTGCACAAA
GCTGATCGAATGAGTATGGCTAACTCACTAGAAGTTCGGACACCGTTGGTTGACCGGGAAGTAGCCAAATTTGCTGCAAC
CATGCCAATTAAGACTCGAATTCATAAAGAAGAAACTAAGGTATCTCTTCGTCGTGCTGCAGAAGCTGAATTACCAGAAC
GAGTAGCTAAGAAGGAAAAACTTGGTTTCCCATCTCCAATCGCCAACTGGATTAAAGAAGATAAGTATCGTAAACGAATT
GAAGCAGCTTTCAATTCAGACATTGCTAAGAAATTCTTTAAGCCAGAAGCTTTAATGCATATTCTTGATGAACATGTTAA
CGGAAAATCAAGTATGCAAAAGATTTTCACAATTTACACATTTATTTTATGGTATGGAGTTTTCTTCCCAGAAGATACCA
ATACTAATACAATAGATTTAGTTAAGAGAACTCAAGTATAG

Upstream 100 bases:

>100_bases
ACGGTAGTAGTATTGGTTTCTAATTTTTGCTAAAATGTTATCTGTTACTGGAATTATAGAACGATATGTATATATTTGAA
AAATAAAAGGGGATTACAAA

Downstream 100 bases:

>100_bases
GTGGAGATGAAATAGATGGACCTTGAAATTGCCACTGTTAAGGCAATCCTTACAGAACATGATTTATTAAAAGAAATCAA
ATCAACTAAAACAGTTGCAA

Product: asparagine synthase (glutamine-hydrolyzing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MCGFAGYINQANVNKDTIKSMADRIKHRGPDDEAFFQDENASMGFRRLSIIDLAHGRQPMMNSAETKVLTFNGEIYNYKQ
VREELKELGYEFRTDVDSEVLIHGYDAWGPELLKKLRGMFAFVIYDKEKQEVFGARDHFGIKPLYYYDDDKTFLWGSEIK
SFLEHPNFVKEFNEELLPIHLSFEFIPSKETMFKNVYKLLPGTYFIHKNGKTETHTYFKFNYDHIDESQTIEEDSKKIRK
IVSDSVDAHMIADVEVGSFLSSGIDSSYVLAEAAKLKPIQSFSLGFRNSKYSELSYSTEFAKEINQKNTPLYMDGDDYFD
ILPTIMYYMDEPLSNPSAMQLFYLSKGTRKNVKVALSGEGADEFFGGYNTYLEAFTFERYQKMVPSFIRKGLAKAVSGLP
RFHGRRFLMRGAQPLSERYYRVNYVFNQQERDQILRNPALNVDSGAYTQHIFDEVKGKDEMTQMQYFDINTWLPFDILHK
ADRMSMANSLEVRTPLVDREVAKFAATMPIKTRIHKEETKVSLRRAAEAELPERVAKKEKLGFPSPIANWIKEDKYRKRI
EAAFNSDIAKKFFKPEALMHILDEHVNGKSSMQKIFTIYTFILWYGVFFPEDTNTNTIDLVKRTQV

Sequences:

>Translated_626_residues
MCGFAGYINQANVNKDTIKSMADRIKHRGPDDEAFFQDENASMGFRRLSIIDLAHGRQPMMNSAETKVLTFNGEIYNYKQ
VREELKELGYEFRTDVDSEVLIHGYDAWGPELLKKLRGMFAFVIYDKEKQEVFGARDHFGIKPLYYYDDDKTFLWGSEIK
SFLEHPNFVKEFNEELLPIHLSFEFIPSKETMFKNVYKLLPGTYFIHKNGKTETHTYFKFNYDHIDESQTIEEDSKKIRK
IVSDSVDAHMIADVEVGSFLSSGIDSSYVLAEAAKLKPIQSFSLGFRNSKYSELSYSTEFAKEINQKNTPLYMDGDDYFD
ILPTIMYYMDEPLSNPSAMQLFYLSKGTRKNVKVALSGEGADEFFGGYNTYLEAFTFERYQKMVPSFIRKGLAKAVSGLP
RFHGRRFLMRGAQPLSERYYRVNYVFNQQERDQILRNPALNVDSGAYTQHIFDEVKGKDEMTQMQYFDINTWLPFDILHK
ADRMSMANSLEVRTPLVDREVAKFAATMPIKTRIHKEETKVSLRRAAEAELPERVAKKEKLGFPSPIANWIKEDKYRKRI
EAAFNSDIAKKFFKPEALMHILDEHVNGKSSMQKIFTIYTFILWYGVFFPEDTNTNTIDLVKRTQV
>Mature_626_residues
MCGFAGYINQANVNKDTIKSMADRIKHRGPDDEAFFQDENASMGFRRLSIIDLAHGRQPMMNSAETKVLTFNGEIYNYKQ
VREELKELGYEFRTDVDSEVLIHGYDAWGPELLKKLRGMFAFVIYDKEKQEVFGARDHFGIKPLYYYDDDKTFLWGSEIK
SFLEHPNFVKEFNEELLPIHLSFEFIPSKETMFKNVYKLLPGTYFIHKNGKTETHTYFKFNYDHIDESQTIEEDSKKIRK
IVSDSVDAHMIADVEVGSFLSSGIDSSYVLAEAAKLKPIQSFSLGFRNSKYSELSYSTEFAKEINQKNTPLYMDGDDYFD
ILPTIMYYMDEPLSNPSAMQLFYLSKGTRKNVKVALSGEGADEFFGGYNTYLEAFTFERYQKMVPSFIRKGLAKAVSGLP
RFHGRRFLMRGAQPLSERYYRVNYVFNQQERDQILRNPALNVDSGAYTQHIFDEVKGKDEMTQMQYFDINTWLPFDILHK
ADRMSMANSLEVRTPLVDREVAKFAATMPIKTRIHKEETKVSLRRAAEAELPERVAKKEKLGFPSPIANWIKEDKYRKRI
EAAFNSDIAKKFFKPEALMHILDEHVNGKSSMQKIFTIYTFILWYGVFFPEDTNTNTIDLVKRTQV

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=404, Percent_Identity=27.7227722772277, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI168229252, Length=404, Percent_Identity=27.7227722772277, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI168229250, Length=404, Percent_Identity=27.7227722772277, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI296010848, Length=380, Percent_Identity=28.1578947368421, Blast_Score=130, Evalue=5e-30,
Organism=Homo sapiens, GI296010852, Length=308, Percent_Identity=26.2987012987013, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI296010850, Length=308, Percent_Identity=26.2987012987013, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786889, Length=366, Percent_Identity=30.8743169398907, Blast_Score=173, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI71993933, Length=401, Percent_Identity=29.6758104738155, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI25147557, Length=401, Percent_Identity=29.6758104738155, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI25147560, Length=301, Percent_Identity=30.2325581395349, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6325403, Length=421, Percent_Identity=27.0783847980998, Blast_Score=123, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6321563, Length=420, Percent_Identity=26.4285714285714, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI45553209, Length=378, Percent_Identity=27.5132275132275, Blast_Score=149, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 72792; Mature: 72792

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGFAGYINQANVNKDTIKSMADRIKHRGPDDEAFFQDENASMGFRRLSIIDLAHGRQPM
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHEEEEECCCCCCCC
MNSAETKVLTFNGEIYNYKQVREELKELGYEFRTDVDSEVLIHGYDAWGPELLKKLRGMF
CCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCHHHHHHHHHHE
AFVIYDKEKQEVFGARDHFGIKPLYYYDDDKTFLWGSEIKSFLEHPNFVKEFNEELLPIH
EEEEECCHHHHHCCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHCCHHHHHHCCCEEEEE
LSFEFIPSKETMFKNVYKLLPGTYFIHKNGKTETHTYFKFNYDHIDESQTIEEDSKKIRK
EEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHH
IVSDSVDAHMIADVEVGSFLSSGIDSSYVLAEAAKLKPIQSFSLGFRNSKYSELSYSTEF
HHHCCCCCEEEEECHHHHHHHCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHH
AKEINQKNTPLYMDGDDYFDILPTIMYYMDEPLSNPSAMQLFYLSKGTRKNVKVALSGEG
HHHHCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEECCC
ADEFFGGYNTYLEAFTFERYQKMVPSFIRKGLAKAVSGLPRFHGRRFLMRGAQPLSERYY
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHCCCHHHHCEE
RVNYVFNQQERDQILRNPALNVDSGAYTQHIFDEVKGKDEMTQMQYFDINTWLPFDILHK
EEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHEEEEECCCCCCHHHHHH
ADRMSMANSLEVRTPLVDREVAKFAATMPIKTRIHKEETKVSLRRAAEAELPERVAKKEK
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGFPSPIANWIKEDKYRKRIEAAFNSDIAKKFFKPEALMHILDEHVNGKSSMQKIFTIYT
CCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHH
FILWYGVFFPEDTNTNTIDLVKRTQV
HHHHHHCCCCCCCCCCEEEHEEECCC
>Mature Secondary Structure
MCGFAGYINQANVNKDTIKSMADRIKHRGPDDEAFFQDENASMGFRRLSIIDLAHGRQPM
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHHEEEEECCCCCCCC
MNSAETKVLTFNGEIYNYKQVREELKELGYEFRTDVDSEVLIHGYDAWGPELLKKLRGMF
CCCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCHHHHHHHHHHE
AFVIYDKEKQEVFGARDHFGIKPLYYYDDDKTFLWGSEIKSFLEHPNFVKEFNEELLPIH
EEEEECCHHHHHCCCCCCCCCCEEEEECCCCEEEEHHHHHHHHHCCHHHHHHCCCEEEEE
LSFEFIPSKETMFKNVYKLLPGTYFIHKNGKTETHTYFKFNYDHIDESQTIEEDSKKIRK
EEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHH
IVSDSVDAHMIADVEVGSFLSSGIDSSYVLAEAAKLKPIQSFSLGFRNSKYSELSYSTEF
HHHCCCCCEEEEECHHHHHHHCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHH
AKEINQKNTPLYMDGDDYFDILPTIMYYMDEPLSNPSAMQLFYLSKGTRKNVKVALSGEG
HHHHCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEECCC
ADEFFGGYNTYLEAFTFERYQKMVPSFIRKGLAKAVSGLPRFHGRRFLMRGAQPLSERYY
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHCCCHHHHCEE
RVNYVFNQQERDQILRNPALNVDSGAYTQHIFDEVKGKDEMTQMQYFDINTWLPFDILHK
EEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHEEEEECCCCCCHHHHHH
ADRMSMANSLEVRTPLVDREVAKFAATMPIKTRIHKEETKVSLRRAAEAELPERVAKKEK
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGFPSPIANWIKEDKYRKRIEAAFNSDIAKKFFKPEALMHILDEHVNGKSSMQKIFTIYT
CCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHH
FILWYGVFFPEDTNTNTIDLVKRTQV
HHHHHHCCCCCCCCCCEEEHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]