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The map label for this gene is ptsI [H]

Identifier: 116492404

GI number: 116492404

Start: 665240

End: 666964

Strand: Direct

Name: ptsI [H]

Synonym: PEPE_0610

Alternate gene names: 116492404

Gene position: 665240-666964 (Clockwise)

Preceding gene: 116492403

Following gene: 116492405

Centisome position: 36.3

GC content: 39.83

Gene sequence:

>1725_bases
ATGGCTGGCACATTAAAAGGTATTGCGGCCAGTGATGGGATTGCCATTGCTAAAGCATATTTGATGGTGGATCCAGATCT
TTCTTTTGAAAAGAAAAACATTACTGACGTTGATGCAGAAATCAAACGCCTTCATGATGCTTTGGAAAAGTCTAAAGAAG
AACTTTCACTCATCAAAGCTAAGGCTGCAGAAAACCTTGGTGCTGAAGAGGCAGAAGTTTTTGAAGCTCATTTAACCATT
CTTTCTGATCCAGAATTGATTGGTAATATTGAAGACAAAATTAAAAACGACACAATTAATGCAGAAGAAGCTACTAAAGA
AGTTACGGATAACTTTATTTCAATGTTTGAAGCAATGACTGATAATGCTTATATGCAAGAACGTGCCGCAGATATTCGTG
ACGTTACTAAACGTTTGATGAGTCACTTGTTGGGTGTGGAACTTCCTAACCCCGCTTTGATTGATTCAGAAGTCGTTATT
GTTGCGCACGACTTGACTCCAAGTGATACAGCTCAATTGGATCGTCGTTACGTTAAAGGCTTTATTACAGATATTGGTGG
TCGTACATCACATTCTGCAATCATGTCTCGTACCCTTGAAATTCCAGCAGTTGTTGGTTCTGAAAAGGCTACTACTAGCA
TAAAACAAGGCGAAATGATGATTGTTGATGGACTTACTGGGGATGCAATTATTGCACCTACTGATGACGAAATCAAAGCT
TATCAACAAAAGGGTGAAGAATTTAAGGCTCAAAAAGCTGAATGGGAAAAACTTAAGAATGAAAAGACCGTTTCAAAAGA
CGGCGTTCATTTTGACTTAGCTGCTAACATCGGTACACCGGATGATTTACAAGGTGTTCTTGACAATGGTGGTGAAGCCG
TTGGTTTGTTCCGTTCTGAATTCTTATACATGAATGCTTCCGAATTACCATCAGAAGATGAACAATTCGAAGCTTATAAG
AAGGTTGTTGAAGGCATGAACGGCAAGCAAGTTGTTGTTCGTACAATGGATATCGGTGGAGACAAGGAACTTCCTTACTT
ACCACTTCCAGAAGAAATGAACCCATTCTTGGGTTATCGTGCAATCCGAATTAGTTTGGATCGTACAGATTTATTCCGTA
CACAACTTCGTGCTTTGCTCCGTGCTTCGGCATTCGGTAAGCTAGCAATTATGTTCCCAATGATTGCTACAGTAGCCGAA
TTTAAGAAGGCCCGTGCTATCTATGATGACGAACGTCAAAAGATGGTCGATGCTGGACAAGAAGTTGGTGAAATCCAAGT
CGGTATGATGATGGAAATCCCAGCTGCTGCTATGATTGCTGATAAGTTGGCTAGGTATGTAGACTTCTTTAGTATTGGAA
CAAACGATTTGATTCAATACTCATTCGCTGCTGATCGTGGTAACGAAAAGGTTTCATACTTGTATCAACCATATAACCCA
TCATTGCTTCGCTTAATCAAGCAAACTATTGATGCTTCACATGCTGAAGGCAAGTGGACTGGTATGTGTGGTGAAATGGC
CGGCGATCAAATCGCTGTTCCAATTTTAATGGGACTTGGTTTAGACGAATTCTCAATGAGTGCAACTTCAATCTTGAAGA
CCCGTTCATTGATGAAGAAGTTAACAACTGACGATATGAAGAAGTTAGCTGAAAAGGCTGTTACAGAAGCTGAAACTAAC
GAAGATGTAATCAAGTTAGTTCAAGAAACTGTTGACAACATTTAA

Upstream 100 bases:

>100_bases
GCCAAGGCGCAGACGTTACTATCTCAGCTGATGGTGATGACGAAGCAGACGCAATCGCAGCACTTGAAGATACAATGAAA
AAGGAAGGACTATCTGACTA

Downstream 100 bases:

>100_bases
TCTAAAGCAAACTATTTTAGAACCGCTGACCTTAGTGTCGCGGTTCTTTTTTATTCTCTAGGAGGAAAATCAATGAAATT
AAAATTAAAGTCGGGAGCCC

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MAGTLKGIAASDGIAIAKAYLMVDPDLSFEKKNITDVDAEIKRLHDALEKSKEELSLIKAKAAENLGAEEAEVFEAHLTI
LSDPELIGNIEDKIKNDTINAEEATKEVTDNFISMFEAMTDNAYMQERAADIRDVTKRLMSHLLGVELPNPALIDSEVVI
VAHDLTPSDTAQLDRRYVKGFITDIGGRTSHSAIMSRTLEIPAVVGSEKATTSIKQGEMMIVDGLTGDAIIAPTDDEIKA
YQQKGEEFKAQKAEWEKLKNEKTVSKDGVHFDLAANIGTPDDLQGVLDNGGEAVGLFRSEFLYMNASELPSEDEQFEAYK
KVVEGMNGKQVVVRTMDIGGDKELPYLPLPEEMNPFLGYRAIRISLDRTDLFRTQLRALLRASAFGKLAIMFPMIATVAE
FKKARAIYDDERQKMVDAGQEVGEIQVGMMMEIPAAAMIADKLARYVDFFSIGTNDLIQYSFAADRGNEKVSYLYQPYNP
SLLRLIKQTIDASHAEGKWTGMCGEMAGDQIAVPILMGLGLDEFSMSATSILKTRSLMKKLTTDDMKKLAEKAVTEAETN
EDVIKLVQETVDNI

Sequences:

>Translated_574_residues
MAGTLKGIAASDGIAIAKAYLMVDPDLSFEKKNITDVDAEIKRLHDALEKSKEELSLIKAKAAENLGAEEAEVFEAHLTI
LSDPELIGNIEDKIKNDTINAEEATKEVTDNFISMFEAMTDNAYMQERAADIRDVTKRLMSHLLGVELPNPALIDSEVVI
VAHDLTPSDTAQLDRRYVKGFITDIGGRTSHSAIMSRTLEIPAVVGSEKATTSIKQGEMMIVDGLTGDAIIAPTDDEIKA
YQQKGEEFKAQKAEWEKLKNEKTVSKDGVHFDLAANIGTPDDLQGVLDNGGEAVGLFRSEFLYMNASELPSEDEQFEAYK
KVVEGMNGKQVVVRTMDIGGDKELPYLPLPEEMNPFLGYRAIRISLDRTDLFRTQLRALLRASAFGKLAIMFPMIATVAE
FKKARAIYDDERQKMVDAGQEVGEIQVGMMMEIPAAAMIADKLARYVDFFSIGTNDLIQYSFAADRGNEKVSYLYQPYNP
SLLRLIKQTIDASHAEGKWTGMCGEMAGDQIAVPILMGLGLDEFSMSATSILKTRSLMKKLTTDDMKKLAEKAVTEAETN
EDVIKLVQETVDNI
>Mature_573_residues
AGTLKGIAASDGIAIAKAYLMVDPDLSFEKKNITDVDAEIKRLHDALEKSKEELSLIKAKAAENLGAEEAEVFEAHLTIL
SDPELIGNIEDKIKNDTINAEEATKEVTDNFISMFEAMTDNAYMQERAADIRDVTKRLMSHLLGVELPNPALIDSEVVIV
AHDLTPSDTAQLDRRYVKGFITDIGGRTSHSAIMSRTLEIPAVVGSEKATTSIKQGEMMIVDGLTGDAIIAPTDDEIKAY
QQKGEEFKAQKAEWEKLKNEKTVSKDGVHFDLAANIGTPDDLQGVLDNGGEAVGLFRSEFLYMNASELPSEDEQFEAYKK
VVEGMNGKQVVVRTMDIGGDKELPYLPLPEEMNPFLGYRAIRISLDRTDLFRTQLRALLRASAFGKLAIMFPMIATVAEF
KKARAIYDDERQKMVDAGQEVGEIQVGMMMEIPAAAMIADKLARYVDFFSIGTNDLIQYSFAADRGNEKVSYLYQPYNPS
LLRLIKQTIDASHAEGKWTGMCGEMAGDQIAVPILMGLGLDEFSMSATSILKTRSLMKKLTTDDMKKLAEKAVTEAETNE
DVIKLVQETVDNI

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=560, Percent_Identity=48.9285714285714, Blast_Score=537, Evalue=1e-154,
Organism=Escherichia coli, GI48994992, Length=520, Percent_Identity=36.5384615384615, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI1788726, Length=495, Percent_Identity=37.5757575757576, Blast_Score=332, Evalue=5e-92,
Organism=Escherichia coli, GI1789193, Length=571, Percent_Identity=32.399299474606, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1787994, Length=421, Percent_Identity=26.3657957244656, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI226510935, Length=222, Percent_Identity=27.4774774774775, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63341; Mature: 63210

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTLKGIAASDGIAIAKAYLMVDPDLSFEKKNITDVDAEIKRLHDALEKSKEELSLIKA
CCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KAAENLGAEEAEVFEAHLTILSDPELIGNIEDKIKNDTINAEEATKEVTDNFISMFEAMT
HHHHCCCCHHHHHHHHHHHEECCCHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
DNAYMQERAADIRDVTKRLMSHLLGVELPNPALIDSEVVIVAHDLTPSDTAQLDRRYVKG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCEEEEEECCCCCHHHHHHHHHHHH
FITDIGGRTSHSAIMSRTLEIPAVVGSEKATTSIKQGEMMIVDGLTGDAIIAPTDDEIKA
HHHHCCCCCHHHHHHHHHHCCCCEECCCHHHHHHCCCCEEEEECCCCCEEECCCCHHHHH
YQQKGEEFKAQKAEWEKLKNEKTVSKDGVHFDLAANIGTPDDLQGVLDNGGEAVGLFRSE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCEEEHHHHH
FLYMNASELPSEDEQFEAYKKVVEGMNGKQVVVRTMDIGGDKELPYLPLPEEMNPFLGYR
HEEECHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHCEE
AIRISLDRTDLFRTQLRALLRASAFGKLAIMFPMIATVAEFKKARAIYDDERQKMVDAGQ
EEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVGEIQVGMMMEIPAAAMIADKLARYVDFFSIGTNDLIQYSFAADRGNEKVSYLYQPYNP
HHCCEEECEEECCCHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCCCCEEEEECCCCH
SLLRLIKQTIDASHAEGKWTGMCGEMAGDQIAVPILMGLGLDEFSMSATSILKTRSLMKK
HHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LTTDDMKKLAEKAVTEAETNEDVIKLVQETVDNI
HCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AGTLKGIAASDGIAIAKAYLMVDPDLSFEKKNITDVDAEIKRLHDALEKSKEELSLIKA
CCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KAAENLGAEEAEVFEAHLTILSDPELIGNIEDKIKNDTINAEEATKEVTDNFISMFEAMT
HHHHCCCCHHHHHHHHHHHEECCCHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
DNAYMQERAADIRDVTKRLMSHLLGVELPNPALIDSEVVIVAHDLTPSDTAQLDRRYVKG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCEEEEEECCCCCHHHHHHHHHHHH
FITDIGGRTSHSAIMSRTLEIPAVVGSEKATTSIKQGEMMIVDGLTGDAIIAPTDDEIKA
HHHHCCCCCHHHHHHHHHHCCCCEECCCHHHHHHCCCCEEEEECCCCCEEECCCCHHHHH
YQQKGEEFKAQKAEWEKLKNEKTVSKDGVHFDLAANIGTPDDLQGVLDNGGEAVGLFRSE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCEEEHHHHH
FLYMNASELPSEDEQFEAYKKVVEGMNGKQVVVRTMDIGGDKELPYLPLPEEMNPFLGYR
HEEECHHHCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHCEE
AIRISLDRTDLFRTQLRALLRASAFGKLAIMFPMIATVAEFKKARAIYDDERQKMVDAGQ
EEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVGEIQVGMMMEIPAAAMIADKLARYVDFFSIGTNDLIQYSFAADRGNEKVSYLYQPYNP
HHCCEEECEEECCCHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCCCCEEEEECCCCH
SLLRLIKQTIDASHAEGKWTGMCGEMAGDQIAVPILMGLGLDEFSMSATSILKTRSLMKK
HHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LTTDDMKKLAEKAVTEAETNEDVIKLVQETVDNI
HCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9172326 [H]