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The map label for this gene is ywbA [H]

Identifier: 116492374

GI number: 116492374

Start: 634637

End: 635863

Strand: Direct

Name: ywbA [H]

Synonym: PEPE_0579

Alternate gene names: 116492374

Gene position: 634637-635863 (Clockwise)

Preceding gene: 116492373

Following gene: 116492375

Centisome position: 34.63

GC content: 33.82

Gene sequence:

>1227_bases
ATGAATAAATTTATGGACGCTCTTAATAATGGTTTTGCACCTAAAGCCCAGAAAGTAGCTAATAATGAATGGATTTTAAC
TATTAAAAATTCAATTTTACAGGTATTACCATTCATCTTTGTTGGTTCTTTAATTACCTTGCTAGGTATTCCCACAAATT
TCTGGGATTGGTGGCCAGATTTATCGCCAATTAGTAGCTTTACTTTTGGTTTAACTTCAATTTTTGTGGCATTTCTTTTT
CCGTATAATTTAATGGAAAGAAAAAAGTTAAATAAACAAAAAATAATTGCTGGTTTATCTTCGGTAGCATTATTTTTGAT
GTTGGCAAATCCTACTTGGGATAAAACAAATTCAATTATTTCTTATCAATTTTCCGAATTAGGTGCTGGAGGAATGTTTG
TTGCGCTAGTTGTTGGTGCGTTTGTTGCTATAATCATGGGCGCTTTTGGGAAATTTAGTTTTTTCGGAGAAGACACTTCT
TTACCAGATTTTGTGATAGCTTGGTTTGATTCGATGCTGCCAATCATGACAGTAATTGGGGTAGGATGGATACTTACGTA
TTTGATTCATTTTGATATTTATCAACTAGTAGTTTCAGCATTTTCTCCATTGTCTAATTCATTAGATACATGGTGGGGAT
TTACGTTATTCCTATTTTTTGACTGTTTCATTTATTCTATGGGGATTTCTGGTTGGGTTTTAGCAGCCATTGATCAACCT
ATAATGTTTGCGGGAATAGCTAACAATGCGCAACAATTTGCTCATGGACAAGCTGTGACGCATGTATTTACTAGTGAAGT
AATTTATAGTTTTCCATGGATTGGTGGTGTTGGATGTACAATGCCTTTAATTCTAATGATGTTACTTTGGGGGAAGTCGA
AGAGACTTAAAGCGTTAGGAAAGGCATGTATAGTACCTTCAATATTCAATATCAATGAACCACTCGTTTTCGGTACAATT
GTTTGGAATCCATATCTTATGCTACCTCTATGGATAAATGGGATTGTGTTACCTTTAATTACTGTAAGTTGGTTCAAGTT
AGGATTATCTCCAATACCACACTCATTAATGCAAATGTGGTATATTCCATTCCCATTCGTTACTTGGATTGTTAGTCCAG
CATTTAGTGCCATTCTTCTTCTTTTGATTTTATTCGTTGTAGGTGGTTTGATATACTATCCATTTTTCAAGGTTTATGAT
AAAAATCTTGTAAAAGAAGAAGGCTAA

Upstream 100 bases:

>100_bases
TACTCCAATTTTAATGGAACCAGATTACTATGCAATTTTAGATGGGGCAGCTGCTATTAAACATATCCAGTCAGAAGTCC
AATAGTTATGGAGGCAAGCT

Downstream 100 bases:

>100_bases
ACAACAAAAAAGAAAGGTCGCATTAAAAGTTATGATAGATAAAGAGAGTAGCTCTACAGTAGCCGTAAAAGAAACCATGG
AAATTATTATTAATGCAGGT

Product: phosphotransferase system cellobiose-specific component IIC

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: Putative PTS system EIIC component [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MNKFMDALNNGFAPKAQKVANNEWILTIKNSILQVLPFIFVGSLITLLGIPTNFWDWWPDLSPISSFTFGLTSIFVAFLF
PYNLMERKKLNKQKIIAGLSSVALFLMLANPTWDKTNSIISYQFSELGAGGMFVALVVGAFVAIIMGAFGKFSFFGEDTS
LPDFVIAWFDSMLPIMTVIGVGWILTYLIHFDIYQLVVSAFSPLSNSLDTWWGFTLFLFFDCFIYSMGISGWVLAAIDQP
IMFAGIANNAQQFAHGQAVTHVFTSEVIYSFPWIGGVGCTMPLILMMLLWGKSKRLKALGKACIVPSIFNINEPLVFGTI
VWNPYLMLPLWINGIVLPLITVSWFKLGLSPIPHSLMQMWYIPFPFVTWIVSPAFSAILLLLILFVVGGLIYYPFFKVYD
KNLVKEEG

Sequences:

>Translated_408_residues
MNKFMDALNNGFAPKAQKVANNEWILTIKNSILQVLPFIFVGSLITLLGIPTNFWDWWPDLSPISSFTFGLTSIFVAFLF
PYNLMERKKLNKQKIIAGLSSVALFLMLANPTWDKTNSIISYQFSELGAGGMFVALVVGAFVAIIMGAFGKFSFFGEDTS
LPDFVIAWFDSMLPIMTVIGVGWILTYLIHFDIYQLVVSAFSPLSNSLDTWWGFTLFLFFDCFIYSMGISGWVLAAIDQP
IMFAGIANNAQQFAHGQAVTHVFTSEVIYSFPWIGGVGCTMPLILMMLLWGKSKRLKALGKACIVPSIFNINEPLVFGTI
VWNPYLMLPLWINGIVLPLITVSWFKLGLSPIPHSLMQMWYIPFPFVTWIVSPAFSAILLLLILFVVGGLIYYPFFKVYD
KNLVKEEG
>Mature_408_residues
MNKFMDALNNGFAPKAQKVANNEWILTIKNSILQVLPFIFVGSLITLLGIPTNFWDWWPDLSPISSFTFGLTSIFVAFLF
PYNLMERKKLNKQKIIAGLSSVALFLMLANPTWDKTNSIISYQFSELGAGGMFVALVVGAFVAIIMGAFGKFSFFGEDTS
LPDFVIAWFDSMLPIMTVIGVGWILTYLIHFDIYQLVVSAFSPLSNSLDTWWGFTLFLFFDCFIYSMGISGWVLAAIDQP
IMFAGIANNAQQFAHGQAVTHVFTSEVIYSFPWIGGVGCTMPLILMMLLWGKSKRLKALGKACIVPSIFNINEPLVFGTI
VWNPYLMLPLWINGIVLPLITVSWFKLGLSPIPHSLMQMWYIPFPFVTWIVSPAFSAILLLLILFVVGGLIYYPFFKVYD
KNLVKEEG

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane [H]

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=455, Percent_Identity=24.8351648351648, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 45813; Mature: 45813

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS51105 PTS_EIIC_TYPE_3 ; PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]