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The map label for this gene is ykwC [H]

Identifier: 116492159

GI number: 116492159

Start: 403003

End: 403866

Strand: Reverse

Name: ykwC [H]

Synonym: PEPE_0356

Alternate gene names: 116492159

Gene position: 403866-403003 (Counterclockwise)

Preceding gene: 116492163

Following gene: 116492158

Centisome position: 22.04

GC content: 40.28

Gene sequence:

>864_bases
ATGAAAATTGGCTTTATTGGAACCGGAGTAATGGGAAGTGGCATTATCCATAATTTATTAAAAAATGGTCACGATGTCGC
TGTTTTTACTCGCACTAAATCTAAAGCAGACCCTTTACTTGAAGAAGGAGCCCAGTGGGCATCTTCTCCCGCCGAGCTAA
CTCAACAAGTGGAAACAGTTATTACCATGGTTGGTTTTCCACAAGATGTGGAAGAAGTTTACTTTGGTGATCACGGGATT
TTTTCAACAGTTACTTCCGGACAAATTTTAATTGATATGACAACTAGTTCGCCCATATTAGCCGAAAAAATTGGTAAAAA
AGCTGATGAACTAGCTGTTATGGCCTTAGATGCTCCTGTTTCTGGTGGCGACATTGGCGCCCAAAATGCTACTTTAACAA
TTATGGTCGGTGGTAACCAGACAGCATTCGAAACCATGAAACCAATCTTTGGTCAAATTGGACATGCAACTAACTATTTT
GGTACTTATGGCGCCGGTCAAAATGCTAAGATGGCTAATCAAATCATGATTGCCGGGACCATGACTGGCTTAACTGAAAT
GTTAGTATACGCTAAAGCTGCCCGGCTTGACTTAAAGACAGTTCTCGAAACTTTATCCAATGGCGGTGCATCAAATTGGA
GTATGGAAAACTACGTTCCACGAATTTTAAAAAATGACTATACTCCGGGATTTTTCGCTAAGCATTTCCTTAAAGATTTA
AAAATTGCACTTGATCAAGCTAAGCAAATGCAAGTTGATTTACCTGCCACGCGTCTGGCCACTCAACTTTATGATAATTT
AGTTAACCAACAGGGATTAGGCGACGATGGAACCCAGGCTTTGATTAAACTCTGGTGGAAATAA

Upstream 100 bases:

>100_bases
GAGTTTAACATCTAAAAGCCCTTTTTTACATAGATATATTGCGTATCGCACTCCAATATCACTATAATTAAGTAAAAATT
CAGATACGGAGGTTTTAATT

Downstream 100 bases:

>100_bases
AAAAGACTCGCAGTAATTACCACGAGCCCTTGCATTCAGTCTCCATGGAGAAGCAGAGTGCTTAATGGCGGCCGTAGTCG
CCATTTAATTTGCGCAAGTT

Product: 3-hydroxyisobutyrate dehydrogenase related beta-hydroxyacid dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKIGFIGTGVMGSGIIHNLLKNGHDVAVFTRTKSKADPLLEEGAQWASSPAELTQQVETVITMVGFPQDVEEVYFGDHGI
FSTVTSGQILIDMTTSSPILAEKIGKKADELAVMALDAPVSGGDIGAQNATLTIMVGGNQTAFETMKPIFGQIGHATNYF
GTYGAGQNAKMANQIMIAGTMTGLTEMLVYAKAARLDLKTVLETLSNGGASNWSMENYVPRILKNDYTPGFFAKHFLKDL
KIALDQAKQMQVDLPATRLATQLYDNLVNQQGLGDDGTQALIKLWWK

Sequences:

>Translated_287_residues
MKIGFIGTGVMGSGIIHNLLKNGHDVAVFTRTKSKADPLLEEGAQWASSPAELTQQVETVITMVGFPQDVEEVYFGDHGI
FSTVTSGQILIDMTTSSPILAEKIGKKADELAVMALDAPVSGGDIGAQNATLTIMVGGNQTAFETMKPIFGQIGHATNYF
GTYGAGQNAKMANQIMIAGTMTGLTEMLVYAKAARLDLKTVLETLSNGGASNWSMENYVPRILKNDYTPGFFAKHFLKDL
KIALDQAKQMQVDLPATRLATQLYDNLVNQQGLGDDGTQALIKLWWK
>Mature_287_residues
MKIGFIGTGVMGSGIIHNLLKNGHDVAVFTRTKSKADPLLEEGAQWASSPAELTQQVETVITMVGFPQDVEEVYFGDHGI
FSTVTSGQILIDMTTSSPILAEKIGKKADELAVMALDAPVSGGDIGAQNATLTIMVGGNQTAFETMKPIFGQIGHATNYF
GTYGAGQNAKMANQIMIAGTMTGLTEMLVYAKAARLDLKTVLETLSNGGASNWSMENYVPRILKNDYTPGFFAKHFLKDL
KIALDQAKQMQVDLPATRLATQLYDNLVNQQGLGDDGTQALIKLWWK

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=269, Percent_Identity=31.5985130111524, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI40556376, Length=263, Percent_Identity=26.9961977186312, Blast_Score=124, Evalue=7e-29,
Organism=Escherichia coli, GI1786719, Length=272, Percent_Identity=37.1323529411765, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI145693186, Length=291, Percent_Identity=31.9587628865979, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1790315, Length=263, Percent_Identity=29.277566539924, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789092, Length=285, Percent_Identity=25.6140350877193, Blast_Score=99, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17557316, Length=269, Percent_Identity=31.5985130111524, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24655230, Length=292, Percent_Identity=33.5616438356164, Blast_Score=144, Evalue=5e-35,
Organism=Drosophila melanogaster, GI19922568, Length=292, Percent_Identity=33.5616438356164, Blast_Score=144, Evalue=5e-35,
Organism=Drosophila melanogaster, GI28574115, Length=263, Percent_Identity=23.9543726235741, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30939; Mature: 30939

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGFIGTGVMGSGIIHNLLKNGHDVAVFTRTKSKADPLLEEGAQWASSPAELTQQVETV
CEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHH
ITMVGFPQDVEEVYFGDHGIFSTVTSGQILIDMTTSSPILAEKIGKKADELAVMALDAPV
HHHHCCCCHHHHHHCCCCCHHHEECCCEEEEEECCCCCHHHHHHCCCHHHEEEEEECCCC
SGGDIGAQNATLTIMVGGNQTAFETMKPIFGQIGHATNYFGTYGAGQNAKMANQIMIAGT
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCEEEEEEH
MTGLTEMLVYAKAARLDLKTVLETLSNGGASNWSMENYVPRILKNDYTPGFFAKHFLKDL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
KIALDQAKQMQVDLPATRLATQLYDNLVNQQGLGDDGTQALIKLWWK
HHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKIGFIGTGVMGSGIIHNLLKNGHDVAVFTRTKSKADPLLEEGAQWASSPAELTQQVETV
CEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHH
ITMVGFPQDVEEVYFGDHGIFSTVTSGQILIDMTTSSPILAEKIGKKADELAVMALDAPV
HHHHCCCCHHHHHHCCCCCHHHEECCCEEEEEECCCCCHHHHHHCCCHHHEEEEEECCCC
SGGDIGAQNATLTIMVGGNQTAFETMKPIFGQIGHATNYFGTYGAGQNAKMANQIMIAGT
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCEEEEEEH
MTGLTEMLVYAKAARLDLKTVLETLSNGGASNWSMENYVPRILKNDYTPGFFAKHFLKDL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
KIALDQAKQMQVDLPATRLATQLYDNLVNQQGLGDDGTQALIKLWWK
HHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]