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The map label for this gene is lacS [H]

Identifier: 116492011

GI number: 116492011

Start: 240309

End: 242255

Strand: Direct

Name: lacS [H]

Synonym: PEPE_0203

Alternate gene names: 116492011

Gene position: 240309-242255 (Clockwise)

Preceding gene: 116492010

Following gene: 116492014

Centisome position: 13.11

GC content: 38.42

Gene sequence:

>1947_bases
ATGGATGCAGAAGTTAAACAAAGCAAAAGTCGTAAAATCACTTCGCGACTAGCTTACTCATTTGGTGCATTTGGTCATGA
TGCGTTTTATGCAACATTATCGACCTATTTTATTATGTTCGTGACTTCCCATTTATTTGATAAGAGTAGTGGGGCTCAAG
GAACTAAAATGATCGCGTACATTACGATGATCATTGCAGCGTTACGTTTCGTAGAACTTGCAATTGATCCACTAATTGGT
AACGCGATTGATAATACCAATTCACGCTGGGGTCATTTCAAGCCTTGGATTGTTACTGGTGGAACGATTGGTTCAGTTGT
TTTAGCACTTTTATTTACCAGTTTAGGTGGTTTAAATACATCTAACCCAATTTTATACTTAATTGTTTTTGCTATTTTAT
ATATTACAATGGATATTTTCTATTCATTTAAAGATGTTGGGTTCTGGTCAATGATTCCTGCGATTTCATTTGATTCAGCA
GAACGTGAAAAAACAGCTACCTATGCTCGGGTTGGTTCTAATATTGGACAAAATATCGTTGGTGTAGTCGTTATGCCAAT
CGTTTTGATGTTTTCAACCAAGGCGAACAGCGGACAAGGTGATAATCGTGGTTGGATGGCCTTCGGGATTATCATCGCTG
CGGTTGCTTTGATTTCAGCTTTAGCTGTAGCAATGGGAACTAAAGAAAATGATTCTGAATTACGTAAAAATACTGAAAAA
ACAACTTTCAAACAAGTTTTCAAAGTATTGGCTCGTAACGACCAATTAATGTGGTTATCTTTAACTTACGGAATTTATAC
GGCCGGTATGGCAATTACTAACTCACTTGAACTTTACTACTTCACTTACATTTTAGGTGATGCTTCGAAATTTACGTTAC
TTGGTTCATTAAACGCCATCATTGGGGTGTTCGCCGTATTGGCCTTCCCACCATTGGCAAAGAAGTTTAGCCGTCGTAAA
GTATTCTTCTTAGCTATTTCAGTCATGATCATTGCTTTGATTATGTTTGCACTTGCCGGACAATCATTACCAATGATCTT
AACTTCTGCCGTACTATTCTACATTCCACAACCACTTATCTTCTTAGTTGTTTTGATGGTTTTAAGTGATTCTGTTGAAT
ATGGACAATTGAAATTTGGTCACCGTGACGAATCATTAACTCTATCTGTTCGTCCTTTACTTGATAAATTAGGTGGAGCT
ATTTCAAACGGTGTAGTTGGTTTAACTGCCGTTTGGGCTGGCATGACCGCTGGAGCAACTGCTGGGGACGTAACTTCCCA
CGGACAAGCTATTTTCAAGTTGATGATGTTTGGTGTTCCAGCAGCTATGATTTTAATTGGAGCCTTCATCTTCTTCAAGA
AGGTTAAATTGGACGAAAAAATGCATGCTGAAATCGTTGACGAGCTGGAAAAAACTTGGTCAACACATTTGGAAACAGAT
GAAACACCTGCAGAAATTGAAAAAGAAGACGGAATTACAACTCTTTACCGTAATCCGGTTTCAGGAAAATTAATCAGTTT
AGACACCGTTGCAGATGAGACATTTGCGAGTGGAAGTATGGGTAAAGGATTTGCAATTAAACCTACCGATGGTCAAGTTT
TAGCTCCATTTGATGGTGAAGTTGTAGCAACTTTCCCAACTCGCCATGCGATTGGACTTCGTTCTGACACAGGCGTCTTA
ACCTTGATTCATATCGGAATTGGTACCGTTGAAATGCGTGGTACGGGATTTGTTCAATATGTTGAAAAAGGCGATCACGT
TAAACAAGGACAAGAATTAATTGAATTCTGGCAGCCTGCAATTAAAAAAGCAGGCCTTGATGATACTGTAATGGTAGTCA
TTACCAACAAGGATATGCCTGAATTTGATTATCTCAAAGAGCTGGGTGAAGACGTTAAGGATAGCGAAGATGTATTGAAG
TTATCCAATACCGGATTATCTAATTAA

Upstream 100 bases:

>100_bases
ACTAAAATAATTAAGCAAATTTTACTAAAAAACATTGACATCCTCTCTGAAAACGATTACATTTAGTGCATAGATAATTA
AAGGAGATGTGGTATAGATT

Downstream 100 bases:

>100_bases
AGTTGGATTTTAAAAGGACAATCAATGATTTATTCATTGATTGTTCTTTTTTTGCATATTAAAAGCCGATGTTGGAGAAT
TTAAATCTTTCCAGCATCGG

Product: Na+/xyloside symporter related transporter

Products: Li (I) [Cytoplasm]; melibiose [Cytoplasm]; Na (I) [Cytoplasm]; melibiose [Cytoplasm]; Proton [Cytoplasm]; melibiose [Cytoplasm] [C]

Alternate protein names: Lactose transport protein; Lactose-proton symporter; Putative phosphotransferase enzyme IIA component; Putative PTS system EIIA component [H]

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MDAEVKQSKSRKITSRLAYSFGAFGHDAFYATLSTYFIMFVTSHLFDKSSGAQGTKMIAYITMIIAALRFVELAIDPLIG
NAIDNTNSRWGHFKPWIVTGGTIGSVVLALLFTSLGGLNTSNPILYLIVFAILYITMDIFYSFKDVGFWSMIPAISFDSA
EREKTATYARVGSNIGQNIVGVVVMPIVLMFSTKANSGQGDNRGWMAFGIIIAAVALISALAVAMGTKENDSELRKNTEK
TTFKQVFKVLARNDQLMWLSLTYGIYTAGMAITNSLELYYFTYILGDASKFTLLGSLNAIIGVFAVLAFPPLAKKFSRRK
VFFLAISVMIIALIMFALAGQSLPMILTSAVLFYIPQPLIFLVVLMVLSDSVEYGQLKFGHRDESLTLSVRPLLDKLGGA
ISNGVVGLTAVWAGMTAGATAGDVTSHGQAIFKLMMFGVPAAMILIGAFIFFKKVKLDEKMHAEIVDELEKTWSTHLETD
ETPAEIEKEDGITTLYRNPVSGKLISLDTVADETFASGSMGKGFAIKPTDGQVLAPFDGEVVATFPTRHAIGLRSDTGVL
TLIHIGIGTVEMRGTGFVQYVEKGDHVKQGQELIEFWQPAIKKAGLDDTVMVVITNKDMPEFDYLKELGEDVKDSEDVLK
LSNTGLSN

Sequences:

>Translated_648_residues
MDAEVKQSKSRKITSRLAYSFGAFGHDAFYATLSTYFIMFVTSHLFDKSSGAQGTKMIAYITMIIAALRFVELAIDPLIG
NAIDNTNSRWGHFKPWIVTGGTIGSVVLALLFTSLGGLNTSNPILYLIVFAILYITMDIFYSFKDVGFWSMIPAISFDSA
EREKTATYARVGSNIGQNIVGVVVMPIVLMFSTKANSGQGDNRGWMAFGIIIAAVALISALAVAMGTKENDSELRKNTEK
TTFKQVFKVLARNDQLMWLSLTYGIYTAGMAITNSLELYYFTYILGDASKFTLLGSLNAIIGVFAVLAFPPLAKKFSRRK
VFFLAISVMIIALIMFALAGQSLPMILTSAVLFYIPQPLIFLVVLMVLSDSVEYGQLKFGHRDESLTLSVRPLLDKLGGA
ISNGVVGLTAVWAGMTAGATAGDVTSHGQAIFKLMMFGVPAAMILIGAFIFFKKVKLDEKMHAEIVDELEKTWSTHLETD
ETPAEIEKEDGITTLYRNPVSGKLISLDTVADETFASGSMGKGFAIKPTDGQVLAPFDGEVVATFPTRHAIGLRSDTGVL
TLIHIGIGTVEMRGTGFVQYVEKGDHVKQGQELIEFWQPAIKKAGLDDTVMVVITNKDMPEFDYLKELGEDVKDSEDVLK
LSNTGLSN
>Mature_648_residues
MDAEVKQSKSRKITSRLAYSFGAFGHDAFYATLSTYFIMFVTSHLFDKSSGAQGTKMIAYITMIIAALRFVELAIDPLIG
NAIDNTNSRWGHFKPWIVTGGTIGSVVLALLFTSLGGLNTSNPILYLIVFAILYITMDIFYSFKDVGFWSMIPAISFDSA
EREKTATYARVGSNIGQNIVGVVVMPIVLMFSTKANSGQGDNRGWMAFGIIIAAVALISALAVAMGTKENDSELRKNTEK
TTFKQVFKVLARNDQLMWLSLTYGIYTAGMAITNSLELYYFTYILGDASKFTLLGSLNAIIGVFAVLAFPPLAKKFSRRK
VFFLAISVMIIALIMFALAGQSLPMILTSAVLFYIPQPLIFLVVLMVLSDSVEYGQLKFGHRDESLTLSVRPLLDKLGGA
ISNGVVGLTAVWAGMTAGATAGDVTSHGQAIFKLMMFGVPAAMILIGAFIFFKKVKLDEKMHAEIVDELEKTWSTHLETD
ETPAEIEKEDGITTLYRNPVSGKLISLDTVADETFASGSMGKGFAIKPTDGQVLAPFDGEVVATFPTRHAIGLRSDTGVL
TLIHIGIGTVEMRGTGFVQYVEKGDHVKQGQELIEFWQPAIKKAGLDDTVMVVITNKDMPEFDYLKELGEDVKDSEDVLK
LSNTGLSN

Specific function: Responsible for transport of beta-galactosides into the cell, with the concomitant uptake of protons (symport system), and also for transport of homologous and heterologous exchange of beta-galactosides [H]

COG id: COG2211

COG function: function code G; Na+/melibiose symporter and related transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790561, Length=482, Percent_Identity=25.3112033195021, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1790159, Length=141, Percent_Identity=45.3900709219858, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI48994989, Length=479, Percent_Identity=22.1294363256785, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1788757, Length=142, Percent_Identity=38.7323943661972, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1786894, Length=98, Percent_Identity=42.8571428571429, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI145693206, Length=346, Percent_Identity=22.2543352601156, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786466, Length=446, Percent_Identity=22.4215246636771, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI87082306, Length=485, Percent_Identity=21.4432989690722, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1787902, Length=400, Percent_Identity=23, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR016196
- InterPro:   IPR001927
- InterPro:   IPR018043
- InterPro:   IPR001127 [H]

Pfam domain/function: PF00358 PTS_EIIA_1 [H]

EC number: NA

Molecular weight: Translated: 70672; Mature: 70672

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS51093 PTS_EIIA_TYPE_1 ; PS00872 NA_GALACTOSIDE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAEVKQSKSRKITSRLAYSFGAFGHDAFYATLSTYFIMFVTSHLFDKSSGAQGTKMIAY
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
ITMIIAALRFVELAIDPLIGNAIDNTNSRWGHFKPWIVTGGTIGSVVLALLFTSLGGLNT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCC
SNPILYLIVFAILYITMDIFYSFKDVGFWSMIPAISFDSAEREKTATYARVGSNIGQNIV
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCHHHH
GVVVMPIVLMFSTKANSGQGDNRGWMAFGIIIAAVALISALAVAMGTKENDSELRKNTEK
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
TTFKQVFKVLARNDQLMWLSLTYGIYTAGMAITNSLELYYFTYILGDASKFTLLGSLNAI
HHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCHHHEEEEEEECCCCCEEHHHHHHHH
IGVFAVLAFPPLAKKFSRRKVFFLAISVMIIALIMFALAGQSLPMILTSAVLFYIPQPLI
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHH
FLVVLMVLSDSVEYGQLKFGHRDESLTLSVRPLLDKLGGAISNGVVGLTAVWAGMTAGAT
HHHHHHHHHCCCCCCCEECCCCCCCEEEEHHHHHHHHCCHHHCCCHHHHHHHHCCCCCCC
AGDVTSHGQAIFKLMMFGVPAAMILIGAFIFFKKVKLDEKMHAEIVDELEKTWSTHLETD
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC
ETPAEIEKEDGITTLYRNPVSGKLISLDTVADETFASGSMGKGFAIKPTDGQVLAPFDGE
CCCHHHHHCCCCEEEECCCCCCCEEEEEHHHHHHHCCCCCCCCEEECCCCCCEECCCCCC
VVATFPTRHAIGLRSDTGVLTLIHIGIGTVEMRGTGFVQYVEKGDHVKQGQELIEFWQPA
EEEECCCCCCCCCCCCCCEEEEEEECCCEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHH
IKKAGLDDTVMVVITNKDMPEFDYLKELGEDVKDSEDVLKLSNTGLSN
HHHCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MDAEVKQSKSRKITSRLAYSFGAFGHDAFYATLSTYFIMFVTSHLFDKSSGAQGTKMIAY
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
ITMIIAALRFVELAIDPLIGNAIDNTNSRWGHFKPWIVTGGTIGSVVLALLFTSLGGLNT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCC
SNPILYLIVFAILYITMDIFYSFKDVGFWSMIPAISFDSAEREKTATYARVGSNIGQNIV
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCHHHH
GVVVMPIVLMFSTKANSGQGDNRGWMAFGIIIAAVALISALAVAMGTKENDSELRKNTEK
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
TTFKQVFKVLARNDQLMWLSLTYGIYTAGMAITNSLELYYFTYILGDASKFTLLGSLNAI
HHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCHHHEEEEEEECCCCCEEHHHHHHHH
IGVFAVLAFPPLAKKFSRRKVFFLAISVMIIALIMFALAGQSLPMILTSAVLFYIPQPLI
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHH
FLVVLMVLSDSVEYGQLKFGHRDESLTLSVRPLLDKLGGAISNGVVGLTAVWAGMTAGAT
HHHHHHHHHCCCCCCCEECCCCCCCEEEEHHHHHHHHCCHHHCCCHHHHHHHHCCCCCCC
AGDVTSHGQAIFKLMMFGVPAAMILIGAFIFFKKVKLDEKMHAEIVDELEKTWSTHLETD
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC
ETPAEIEKEDGITTLYRNPVSGKLISLDTVADETFASGSMGKGFAIKPTDGQVLAPFDGE
CCCHHHHHCCCCEEEECCCCCCCEEEEEHHHHHHHCCCCCCCCEEECCCCCCEECCCCCC
VVATFPTRHAIGLRSDTGVLTLIHIGIGTVEMRGTGFVQYVEKGDHVKQGQELIEFWQPA
EEEECCCCCCCCCCCCCCEEEEEEECCCEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHH
IKKAGLDDTVMVVITNKDMPEFDYLKELGEDVKDSEDVLKLSNTGLSN
HHHCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Li (I) [Periplasm]; melibiose [Periplasm]; Na (I) [Periplasm]; melibiose [Periplasm]; Proton [Periplasm]; melibiose [Periplasm] [C]

Specific reaction: Li (I) [Periplasm] + melibiose [Periplasm] = Li (I) [Cytoplasm] + melibiose [Cytoplasm] Na (I) [Periplasm] + melibiose [Periplasm] = Na (I) [Cytoplasm] + melibiose [Cytoplasm] Proton [Periplasm] + melibiose [Periplasm] = Proton [Cytoplasm] + melibiose [Cy

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2644191; 1694527 [H]