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The map label for this gene is ptsG [H]

Identifier: 116491895

GI number: 116491895

Start: 85747

End: 86238

Strand: Direct

Name: ptsG [H]

Synonym: PEPE_0077

Alternate gene names: 116491895

Gene position: 85747-86238 (Clockwise)

Preceding gene: 116491894

Following gene: 116491896

Centisome position: 4.68

GC content: 38.21

Gene sequence:

>492_bases
ATGTTCGGCATTACAAAAAAGAAAAGTAATATTTTTGTTGTAGCTACCCCTATCGAAGGTGTTTGCATACCAATTACAGC
TGTTTCAGATGAAGTATTTGCACAAAAAATGATGGGTGATGGATTTGCTATCAAACCAACCAATAATGCGAATAAGGTTG
TTGCGCCTATTAGCGGGAAAGTTGTTTCATTACCTGAAAGTAAACATGCTATTGGAATTACGGATGAGGTACGAGGAATA
TCTGTATTGGTCCATGTTGGTATAGATACAGTTCGTTTAAAGGGAAAAGGATTTAAAACATTCGTTGAAGTGGGGCAAGA
GGTTAAAGTGGGAACCCAAATAGCAACAATTAACAGAGCAACGTTAGATGCAGAAGATTTTGATTTGACGACGATGGTTG
TCTTTACGGAAGGCTATACAGGTGAAATACCGCTTGGCAGCAAACTTAATCAACAACTTGCTTTGGGTACATTGGTTCTC
AAACAAGCGTAA

Upstream 100 bases:

>100_bases
TTGTAACTCATACTCTTTGAGGCATTACCTTTTGCTTGTTGAAATATACGGGTGGAATGGGTGTCGCAAGGAGTTTTTTT
GTATTAAGGAAGTGGTAAGA

Downstream 100 bases:

>100_bases
GTAATATTTAAGCAGGTAAGGAGGTGTAAATGAGATGCTGGTGGTGAAAAAAATAAATAATAATGTGGTGGTCTGTTTGG
ATCGGAATGGAGCACAGCTA

Product: phosphotransferase system IIA component

Products: NA

Alternate protein names: EII-Glc/EIII-Glc; EIICBA-Glc; Glucose permease IIC component; PTS system glucose-specific EIIC component; Glucose-specific phosphotransferase enzyme IIB component; PTS system glucose-specific EIIB component; Glucose-specific phosphotransferase enzyme IIA component; PTS system glucose-specific EIIA component [H]

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MFGITKKKSNIFVVATPIEGVCIPITAVSDEVFAQKMMGDGFAIKPTNNANKVVAPISGKVVSLPESKHAIGITDEVRGI
SVLVHVGIDTVRLKGKGFKTFVEVGQEVKVGTQIATINRATLDAEDFDLTTMVVFTEGYTGEIPLGSKLNQQLALGTLVL
KQA

Sequences:

>Translated_163_residues
MFGITKKKSNIFVVATPIEGVCIPITAVSDEVFAQKMMGDGFAIKPTNNANKVVAPISGKVVSLPESKHAIGITDEVRGI
SVLVHVGIDTVRLKGKGFKTFVEVGQEVKVGTQIATINRATLDAEDFDLTTMVVFTEGYTGEIPLGSKLNQQLALGTLVL
KQA
>Mature_163_residues
MFGITKKKSNIFVVATPIEGVCIPITAVSDEVFAQKMMGDGFAIKPTNNANKVVAPISGKVVSLPESKHAIGITDEVRGI
SVLVHVGIDTVRLKGKGFKTFVEVGQEVKVGTQIATINRATLDAEDFDLTTMVVFTEGYTGEIPLGSKLNQQLALGTLVL
KQA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788757, Length=131, Percent_Identity=41.2213740458015, Blast_Score=91, Evalue=3e-20,
Organism=Escherichia coli, GI1790159, Length=136, Percent_Identity=36.0294117647059, Blast_Score=90, Evalue=6e-20,
Organism=Escherichia coli, GI1786894, Length=119, Percent_Identity=40.3361344537815, Blast_Score=84, Evalue=4e-18,

Paralogues:

None

Copy number: 3540 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR011535 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17330; Mature: 17330

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGITKKKSNIFVVATPIEGVCIPITAVSDEVFAQKMMGDGFAIKPTNNANKVVAPISGK
CCCCEECCCCEEEEEECCCCEEEEEEECCHHHHHHHHHCCCEEEEECCCCCEEEEECCCE
VVSLPESKHAIGITDEVRGISVLVHVGIDTVRLKGKGFKTFVEVGQEVKVGTQIATINRA
EEECCCCCCEECCCCCCCEEEEEEEECCEEEEEECCCCHHHHHCCCCEECCEEEEEEEEE
TLDAEDFDLTTMVVFTEGYTGEIPLGSKLNQQLALGTLVLKQA
EECCCCCCEEEEEEEECCCCCCCCCCCCCCCHHEEEEEEEECC
>Mature Secondary Structure
MFGITKKKSNIFVVATPIEGVCIPITAVSDEVFAQKMMGDGFAIKPTNNANKVVAPISGK
CCCCEECCCCEEEEEECCCCEEEEEEECCHHHHHHHHHCCCEEEEECCCCCEEEEECCCE
VVSLPESKHAIGITDEVRGISVLVHVGIDTVRLKGKGFKTFVEVGQEVKVGTQIATINRA
EEECCCCCCEECCCCCCCEEEEEEEECCEEEEEECCCCHHHHHCCCCEECCEEEEEEEEE
TLDAEDFDLTTMVVFTEGYTGEIPLGSKLNQQLALGTLVLKQA
EECCCCCCEEEEEEEECCCCCCCCCCCCCCCHHEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7670643 [H]