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The map label for this gene is lacA [C]

Identifier: 116491867

GI number: 116491867

Start: 45891

End: 46445

Strand: Direct

Name: lacA [C]

Synonym: PEPE_0049

Alternate gene names: 116491867

Gene position: 45891-46445 (Clockwise)

Preceding gene: 116491864

Following gene: 116491868

Centisome position: 2.5

GC content: 41.8

Gene sequence:

>555_bases
ATGCAATCCAAAATGGATTTGGGAACAGCGGCCTATGATGGTGTCGAAGAAATCGTAGCTAAAAACCAACGGTTGGTTCA
GCAATTAAATACTGGAGTTCATTCGGCAAATGAAGTACTTAGCATTGTAAGCCAAATCACTAACGAGCGAATTGATGGTT
CGACTGAAATTCGATTACCTTTTCAAACCGATTTTGGTCGCAATCTCCACATTGGTAAAAAAGTTTTCATTAATAGCGGA
TCGATGTTTGTGGACTTAGGTGGTATCTATATTGGTGATCACGTCTTGATTGGTCCAAACGTAACGATTGCTTCCGTTAA
TCACCGTCTAGAACCTAGCGAGCGCCGTAATCTAGATTTATACTCGGTTTGTATTCATAATAATGCTTGGTTAGGCGCCA
ATGTGACGGTAACTCCTGGCGTAATTATTGGTGAAAATGCAGTAGTTGCTGCGGGCGCCGTGGTTACCCGCGATGTTCCA
CCCAATGCGGTAGTTGCTGGTATTCCAGCACGTGTAATTAAAATAATCAAGAGTACTAAGGAGGATCAAAAATAA

Upstream 100 bases:

>100_bases
CTAAAAGTTATTGTTTTTGATGTTTAATAAGTATTTAACATTATAGTAACGATCCCGTAGACTAGAGTCAATCAATAAGG
TTAGGAAGCGGGGTGAGTTA

Downstream 100 bases:

>100_bases
TGAGTAATAAGAATAAAGTAGTAATCATCATGGGGGCTTCGAGTGGAATCGGAGCTGCGACAGCAGCAATGCTAGCGCAA
CAAGGAGCTAAGCTTAGCTT

Product: galactoside O-acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MQSKMDLGTAAYDGVEEIVAKNQRLVQQLNTGVHSANEVLSIVSQITNERIDGSTEIRLPFQTDFGRNLHIGKKVFINSG
SMFVDLGGIYIGDHVLIGPNVTIASVNHRLEPSERRNLDLYSVCIHNNAWLGANVTVTPGVIIGENAVVAAGAVVTRDVP
PNAVVAGIPARVIKIIKSTKEDQK

Sequences:

>Translated_184_residues
MQSKMDLGTAAYDGVEEIVAKNQRLVQQLNTGVHSANEVLSIVSQITNERIDGSTEIRLPFQTDFGRNLHIGKKVFINSG
SMFVDLGGIYIGDHVLIGPNVTIASVNHRLEPSERRNLDLYSVCIHNNAWLGANVTVTPGVIIGENAVVAAGAVVTRDVP
PNAVVAGIPARVIKIIKSTKEDQK
>Mature_184_residues
MQSKMDLGTAAYDGVEEIVAKNQRLVQQLNTGVHSANEVLSIVSQITNERIDGSTEIRLPFQTDFGRNLHIGKKVFINSG
SMFVDLGGIYIGDHVLIGPNVTIASVNHRLEPSERRNLDLYSVCIHNNAWLGANVTVTPGVIIGENAVVAAGAVVTRDVP
PNAVVAGIPARVIKIIKSTKEDQK

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=172, Percent_Identity=35.4651162790698, Blast_Score=113, Evalue=9e-27,
Organism=Escherichia coli, GI1786664, Length=168, Percent_Identity=37.5, Blast_Score=103, Evalue=5e-24,
Organism=Escherichia coli, GI1788345, Length=122, Percent_Identity=36.0655737704918, Blast_Score=65, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322243, Length=145, Percent_Identity=39.3103448275862, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 19820; Mature: 19820

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSKMDLGTAAYDGVEEIVAKNQRLVQQLNTGVHSANEVLSIVSQITNERIDGSTEIRLP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEE
FQTDFGRNLHIGKKVFINSGSMFVDLGGIYIGDHVLIGPNVTIASVNHRLEPSERRNLDL
ECCCCCCCEECCCEEEEECCCEEEEECCEEECCEEEECCCEEEEECCCCCCCCHHCCCEE
YSVCIHNNAWLGANVTVTPGVIIGENAVVAAGAVVTRDVPPNAVVAGIPARVIKIIKSTK
EEEEEECCCEECCCEEECCCEEECCCCEEEECEEEECCCCCCCEEECCHHHHHHHHHHHH
EDQK
CCCC
>Mature Secondary Structure
MQSKMDLGTAAYDGVEEIVAKNQRLVQQLNTGVHSANEVLSIVSQITNERIDGSTEIRLP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEE
FQTDFGRNLHIGKKVFINSGSMFVDLGGIYIGDHVLIGPNVTIASVNHRLEPSERRNLDL
ECCCCCCCEECCCEEEEECCCEEEEECCEEECCEEEECCCEEEEECCCCCCCCHHCCCEE
YSVCIHNNAWLGANVTVTPGVIIGENAVVAAGAVVTRDVPPNAVVAGIPARVIKIIKSTK
EEEEEECCCEECCCEEECCCEEECCCCEEEECEEEECCCCCCCEEECCHHHHHHHHHHHH
EDQK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA