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The map label for this gene is metF [H]

Identifier: 116332450

GI number: 116332450

Start: 5538

End: 6410

Strand: Direct

Name: metF [H]

Synonym: LBJ_4004

Alternate gene names: 116332450

Gene position: 5538-6410 (Clockwise)

Preceding gene: 116332449

Following gene: 116332452

Centisome position: 1.85

GC content: 40.44

Gene sequence:

>873_bases
ATGAAAAAAGTATCTGAAATTTACGGTTCTGCAAAAGGGCCTGTGTATTCGTTTGAATTCTTTCCTCCGAAAACTCAGGA
TGGAGATTTAAAATTGATGGGAACCGTGAAGGAGTTGGCCCGTCTTGATCCGGACTTTGTGACCGTGACGTATGGCGCCG
GCGGTTCGACAAGAGATAAAACCGCACAAATCTTATCTGAGATTTCGAAAAATTATTCTTTTCCGACGGTTTCTCATTTT
ACTTGTGTCGGAGCAAATCAAGATCAGATTTTTAAAACCCTAAAAGAAATTCGTTCTTCCGGGATTGTAAATTTGATGGC
TCTTCGCGGAGATCCCCCGAAAGGAGAGGGAAAATTTAAAAAGACGGAGGGTGGTTTTGAGAATGCGACGGAACTCATTT
CATTCATTCGTTCTGAAAAGTTGGATTTTTGCATCGGAGGTGGTTGTTACCCCGAAAAACACCCGAATGCAAAAAGTCTT
GAAGAAGACGTCAGAAATTTAAAACGGAAAGTGGATGCCGGGACGGACTTTTTAGTTTCCCAGCTTTTTTTTGTGAATTC
CATCTTTGAAAACTTTTTGAATTTGGTAAGAAAGGCAGATATTCGTGCTCCTGTAATTCCGGGAATTATGCCGATCACTT
CTTTTTCTCAAATCGAAAGATTTCGTTCGATAGCCGGTTGTGAGTTCCCCTCTTCTCTCATTGAAGATCTGCAAGAAGTA
GAACATCGCCCAGAGGAGTTTTACAGAAGAAGTTTAAACTTTTCTATAAAACAATGTAGGGAGTTGCTTGCGATGGGAGT
TCCGGGAATTCATCTTTATACTCTGAATCAATCTCACGCGAGTTACGATATTGTAAGGGAATTAAAAAGTTAA

Upstream 100 bases:

>100_bases
TCCTTGCGCCCGGGCTCCCTTTTTGGAAAGGCTTTGACCCCTCTTCGCAGCGGGCGAGGACAAATCCAAATTCTCCTTTG
CTTTCAATGATCAGGAGTTT

Downstream 100 bases:

>100_bases
AATCTGGATTTTTTGTTTTCGTCTGTCTTATACATTCCAAAGAGGCGTATTTTGTGAGAAATATCGTGAACTTACTTAAC
GTGAGTTCACAAATGCGAAA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MKKVSEIYGSAKGPVYSFEFFPPKTQDGDLKLMGTVKELARLDPDFVTVTYGAGGSTRDKTAQILSEISKNYSFPTVSHF
TCVGANQDQIFKTLKEIRSSGIVNLMALRGDPPKGEGKFKKTEGGFENATELISFIRSEKLDFCIGGGCYPEKHPNAKSL
EEDVRNLKRKVDAGTDFLVSQLFFVNSIFENFLNLVRKADIRAPVIPGIMPITSFSQIERFRSIAGCEFPSSLIEDLQEV
EHRPEEFYRRSLNFSIKQCRELLAMGVPGIHLYTLNQSHASYDIVRELKS

Sequences:

>Translated_290_residues
MKKVSEIYGSAKGPVYSFEFFPPKTQDGDLKLMGTVKELARLDPDFVTVTYGAGGSTRDKTAQILSEISKNYSFPTVSHF
TCVGANQDQIFKTLKEIRSSGIVNLMALRGDPPKGEGKFKKTEGGFENATELISFIRSEKLDFCIGGGCYPEKHPNAKSL
EEDVRNLKRKVDAGTDFLVSQLFFVNSIFENFLNLVRKADIRAPVIPGIMPITSFSQIERFRSIAGCEFPSSLIEDLQEV
EHRPEEFYRRSLNFSIKQCRELLAMGVPGIHLYTLNQSHASYDIVRELKS
>Mature_290_residues
MKKVSEIYGSAKGPVYSFEFFPPKTQDGDLKLMGTVKELARLDPDFVTVTYGAGGSTRDKTAQILSEISKNYSFPTVSHF
TCVGANQDQIFKTLKEIRSSGIVNLMALRGDPPKGEGKFKKTEGGFENATELISFIRSEKLDFCIGGGCYPEKHPNAKSL
EEDVRNLKRKVDAGTDFLVSQLFFVNSIFENFLNLVRKADIRAPVIPGIMPITSFSQIERFRSIAGCEFPSSLIEDLQEV
EHRPEEFYRRSLNFSIKQCRELLAMGVPGIHLYTLNQSHASYDIVRELKS

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=281, Percent_Identity=33.0960854092527, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1790377, Length=272, Percent_Identity=34.1911764705882, Blast_Score=172, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI25147441, Length=285, Percent_Identity=33.3333333333333, Blast_Score=157, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI25147436, Length=281, Percent_Identity=33.4519572953737, Blast_Score=157, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6325234, Length=298, Percent_Identity=32.8859060402685, Blast_Score=158, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6321313, Length=294, Percent_Identity=31.2925170068027, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24662658, Length=200, Percent_Identity=26, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 32544; Mature: 32544

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVSEIYGSAKGPVYSFEFFPPKTQDGDLKLMGTVKELARLDPDFVTVTYGAGGSTRDK
CCCHHHHHCCCCCCEEEEECCCCCCCCCCEEHHHHHHHHHHCCCCEEEEEECCCCCCHHH
TAQILSEISKNYSFPTVSHFTCVGANQDQIFKTLKEIRSSGIVNLMALRGDPPKGEGKFK
HHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
KTEGGFENATELISFIRSEKLDFCIGGGCYPEKHPNAKSLEEDVRNLKRKVDAGTDFLVS
CCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
QLFFVNSIFENFLNLVRKADIRAPVIPGIMPITSFSQIERFRSIAGCEFPSSLIEDLQEV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EHRPEEFYRRSLNFSIKQCRELLAMGVPGIHLYTLNQSHASYDIVRELKS
HCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure
MKKVSEIYGSAKGPVYSFEFFPPKTQDGDLKLMGTVKELARLDPDFVTVTYGAGGSTRDK
CCCHHHHHCCCCCCEEEEECCCCCCCCCCEEHHHHHHHHHHCCCCEEEEEECCCCCCHHH
TAQILSEISKNYSFPTVSHFTCVGANQDQIFKTLKEIRSSGIVNLMALRGDPPKGEGKFK
HHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
KTEGGFENATELISFIRSEKLDFCIGGGCYPEKHPNAKSLEEDVRNLKRKVDAGTDFLVS
CCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
QLFFVNSIFENFLNLVRKADIRAPVIPGIMPITSFSQIERFRSIAGCEFPSSLIEDLQEV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EHRPEEFYRRSLNFSIKQCRELLAMGVPGIHLYTLNQSHASYDIVRELKS
HCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]