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The map label for this gene is algU [H]

Identifier: 116329426

GI number: 116329426

Start: 3445191

End: 3445790

Strand: Direct

Name: algU [H]

Synonym: LBL_2894

Alternate gene names: 116329426

Gene position: 3445191-3445790 (Clockwise)

Preceding gene: 116329425

Following gene: 116329427

Centisome position: 95.32

GC content: 36.33

Gene sequence:

>600_bases
ATGAGGGAAAACACTCCAATTGTATGCAACCAAGAGGACTGGGATTGCATTCAAAAAGTTTTACACGGCGATTTTAATTC
CTTTGAAATACTGATGAACCGGTATCAAGGACTGATTTACTCTCAAGTGATCAAAGCCTTTCGAAACGAAACCGAAGCTG
AAGATTTTACTCAGGATATTTTCTTAAAAGCCTTTGAAAGTCTATCTACATTTCAAGGTAGGTCCCAATTCTCCACTTGG
TTGTTCGTAATCGCAAGAAACGAAATTATTCGAAGGTATCGTAGAGAACATCCCGAAATATCAGGACTCGATACGCTTCT
ACTTACGGAAAGTGAAGTAGAAAAGAAAAACGAAACCTCATCCGAACAGGAAACAAAACTTCTGAAACAGGAAATTTCCG
AGAAAATCAGAAGCCTCGTGGAAAGTCTACCGGAACTCTATCGTAAACCGATCATGCTCCATTATTTCGAGAATATGTCC
TATAAAGAAATTTCCCAAAAATTAAACTTGAAAATGAACACTTTAAAGAGTTATATTTTTAGGGGAAAGGAAATCATGAG
AGATTGGTTAAACAAAGAAGAGAATGAAAAACAAGAATAA

Upstream 100 bases:

>100_bases
TCGGATTCATTTCCGTATTAGGACAATCTCTCGCCATGTTAGGCGGACTTGTTCCGCTCGGGACCGGCCTGGGATTACTC
TGTTATTATAAATTCTCCCG

Downstream 100 bases:

>100_bases
TTCCCATTCTAAAGAAATAGATTTCGTCCCGACCGAACTCGAAGAAAAGCTGCATTTTTTCGAAGAAGAACCTCTCAATC
CTCCGGAAAATACGACGAAG

Product: RNA polymerase sigma subunit

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MRENTPIVCNQEDWDCIQKVLHGDFNSFEILMNRYQGLIYSQVIKAFRNETEAEDFTQDIFLKAFESLSTFQGRSQFSTW
LFVIARNEIIRRYRREHPEISGLDTLLLTESEVEKKNETSSEQETKLLKQEISEKIRSLVESLPELYRKPIMLHYFENMS
YKEISQKLNLKMNTLKSYIFRGKEIMRDWLNKEENEKQE

Sequences:

>Translated_199_residues
MRENTPIVCNQEDWDCIQKVLHGDFNSFEILMNRYQGLIYSQVIKAFRNETEAEDFTQDIFLKAFESLSTFQGRSQFSTW
LFVIARNEIIRRYRREHPEISGLDTLLLTESEVEKKNETSSEQETKLLKQEISEKIRSLVESLPELYRKPIMLHYFENMS
YKEISQKLNLKMNTLKSYIFRGKEIMRDWLNKEENEKQE
>Mature_199_residues
MRENTPIVCNQEDWDCIQKVLHGDFNSFEILMNRYQGLIYSQVIKAFRNETEAEDFTQDIFLKAFESLSTFQGRSQFSTW
LFVIARNEIIRRYRREHPEISGLDTLLLTESEVEKKNETSSEQETKLLKQEISEKIRSLVESLPELYRKPIMLHYFENMS
YKEISQKLNLKMNTLKSYIFRGKEIMRDWLNKEENEKQE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=178, Percent_Identity=30.3370786516854, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 23926; Mature: 23926

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRENTPIVCNQEDWDCIQKVLHGDFNSFEILMNRYQGLIYSQVIKAFRNETEAEDFTQDI
CCCCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FLKAFESLSTFQGRSQFSTWLFVIARNEIIRRYRREHPEISGLDTLLLTESEVEKKNETS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHCCCC
SEQETKLLKQEISEKIRSLVESLPELYRKPIMLHYFENMSYKEISQKLNLKMNTLKSYIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHCCHHHHHHHHHH
RGKEIMRDWLNKEENEKQE
CCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRENTPIVCNQEDWDCIQKVLHGDFNSFEILMNRYQGLIYSQVIKAFRNETEAEDFTQDI
CCCCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FLKAFESLSTFQGRSQFSTWLFVIARNEIIRRYRREHPEISGLDTLLLTESEVEKKNETS
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHCCCC
SEQETKLLKQEISEKIRSLVESLPELYRKPIMLHYFENMSYKEISQKLNLKMNTLKSYIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHCCHHHHHHHHHH
RGKEIMRDWLNKEENEKQE
CCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]