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The map label for this gene is pldB-1 [H]

Identifier: 116329372

GI number: 116329372

Start: 3373270

End: 3374214

Strand: Direct

Name: pldB-1 [H]

Synonym: LBL_2828

Alternate gene names: 116329372

Gene position: 3373270-3374214 (Clockwise)

Preceding gene: 116329369

Following gene: 116329373

Centisome position: 93.33

GC content: 40.42

Gene sequence:

>945_bases
ATGAATTTTTTCCAAATGAAATTTTTTTCTCTTTTCAAAGGATCGTTTGTTTTTATAACACGTCTCGCTATGTCATGGAT
TAATTTTTACAATCTAGAAGACGATATCTTTGTGGGAAGTAAAGGAACCAAGATTTTCTATCGAACCTACCAACCCAAGG
AAGGTAGGAAAGGGAACCGGGTACTCGTGGTACAACACGGGATAGGAGAGCACAGCGGACGCTATGAATTCTTAGTAGAA
GCTTTCGCTGGAACCGGAACAGCGTCTTATCTTATCGACTCTCACGGACACGGACGTTCTGAAGGAAAACGAGGCGCAGT
GGATTCTTTTTCGGATTATCTTTCCGACTTGGATAAACTCATTGAAATCGCAAAGAAGAAAGAAAAAGTTTCCAAAGTTA
CTCTTCTGGGTCATTCGATGGGAGCCGCCATTTCCACTTTCTATGCAGAGGAAGGAACAAATCAAGGTAATTTGAATGCG
CTGATTATTTCCGCCCTTCCTATAAAAGTAAAATTGGATCTCGTGATGAAACTCAAAAAAGGAATCGCGCCTTTTATGGC
CGACATCTTTCCGAATCTGACTCTTCCGACCGGTTTGAATGTAAATCACTTAAGTCACGATAAGGCAGTAGTCGACGCTT
ATGTGAAGGACCCTTTAGTTCATGGAATGGCTTCGACCTATCTCGGAAACATGCTTCTAAATAGCGAAGAACCGATTCTC
GCCAACGCAGGAAAAATCAAAATTCCAATTTATATTTTTCACGGAAAAGAAGACCAAATAGCCGATTCCGCCGGAAGTGA
GGTTTTTTTTGAAGTTGTAGGTTCTTCCGATAAAACCCTCAAAATTTACGAAGGACTTTATCACGAAACAATGAACGAAC
GTATAGAAGACAGAACCAAGGTTTTAACCGATTTAAAGAAATGGTTTGAATCTCACGTAAACTGA

Upstream 100 bases:

>100_bases
TCAAAAACATTCCGCAATGGAAAAATGTTCCGTAGGAAGACATGAGTCCAGGAAAATCGTTTCGTTTTGTGAGACAACAG
AAAAGAATCTTCTTATTGGA

Downstream 100 bases:

>100_bases
CAGACAGAACGTTTGTTCTGTTTTTCTCTAAAAAAAATCATCGACCGGGACCTCCCCTTTGGTAGGATTGAATTCGGTCA
AAAGCCTTTTTTAAAAATAA

Product: lysophospholipase

Products: glycerophosphocholine; carboxylate

Alternate protein names: Bacilysocin biosynthesis protein ytpA [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MNFFQMKFFSLFKGSFVFITRLAMSWINFYNLEDDIFVGSKGTKIFYRTYQPKEGRKGNRVLVVQHGIGEHSGRYEFLVE
AFAGTGTASYLIDSHGHGRSEGKRGAVDSFSDYLSDLDKLIEIAKKKEKVSKVTLLGHSMGAAISTFYAEEGTNQGNLNA
LIISALPIKVKLDLVMKLKKGIAPFMADIFPNLTLPTGLNVNHLSHDKAVVDAYVKDPLVHGMASTYLGNMLLNSEEPIL
ANAGKIKIPIYIFHGKEDQIADSAGSEVFFEVVGSSDKTLKIYEGLYHETMNERIEDRTKVLTDLKKWFESHVN

Sequences:

>Translated_314_residues
MNFFQMKFFSLFKGSFVFITRLAMSWINFYNLEDDIFVGSKGTKIFYRTYQPKEGRKGNRVLVVQHGIGEHSGRYEFLVE
AFAGTGTASYLIDSHGHGRSEGKRGAVDSFSDYLSDLDKLIEIAKKKEKVSKVTLLGHSMGAAISTFYAEEGTNQGNLNA
LIISALPIKVKLDLVMKLKKGIAPFMADIFPNLTLPTGLNVNHLSHDKAVVDAYVKDPLVHGMASTYLGNMLLNSEEPIL
ANAGKIKIPIYIFHGKEDQIADSAGSEVFFEVVGSSDKTLKIYEGLYHETMNERIEDRTKVLTDLKKWFESHVN
>Mature_314_residues
MNFFQMKFFSLFKGSFVFITRLAMSWINFYNLEDDIFVGSKGTKIFYRTYQPKEGRKGNRVLVVQHGIGEHSGRYEFLVE
AFAGTGTASYLIDSHGHGRSEGKRGAVDSFSDYLSDLDKLIEIAKKKEKVSKVTLLGHSMGAAISTFYAEEGTNQGNLNA
LIISALPIKVKLDLVMKLKKGIAPFMADIFPNLTLPTGLNVNHLSHDKAVVDAYVKDPLVHGMASTYLGNMLLNSEEPIL
ANAGKIKIPIYIFHGKEDQIADSAGSEVFFEVVGSSDKTLKIYEGLYHETMNERIEDRTKVLTDLKKWFESHVN

Specific function: Phospholipase involved in the biosynthesis of the antibiotic bacilysocin. It probably catalyzes the hydrolysis of the 2-sn-acyl moiety of phosphatidylglycerol to produce bacilysocin (lysophosphatidylglycerol). Is also able to catalyze the hydrolysis react

COG id: COG2267

COG function: function code I; Lysophospholipase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily [H]

Homologues:

Organism=Homo sapiens, GI51242953, Length=274, Percent_Identity=32.1167883211679, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI6005786, Length=274, Percent_Identity=32.1167883211679, Blast_Score=131, Evalue=8e-31,
Organism=Escherichia coli, GI1787678, Length=259, Percent_Identity=25.4826254826255, Blast_Score=91, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6322756, Length=296, Percent_Identity=28.3783783783784, Blast_Score=99, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022742 [H]

Pfam domain/function: PF12146 Hydrolase_4 [H]

EC number: 3.1.1.5

Molecular weight: Translated: 35092; Mature: 35092

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFFQMKFFSLFKGSFVFITRLAMSWINFYNLEDDIFVGSKGTKIFYRTYQPKEGRKGNR
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEECCCCCCCCCE
VLVVQHGIGEHSGRYEFLVEAFAGTGTASYLIDSHGHGRSEGKRGAVDSFSDYLSDLDKL
EEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
IEIAKKKEKVSKVTLLGHSMGAAISTFYAEEGTNQGNLNALIISALPIKVKLDLVMKLKK
HHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCEEEHHHHHHHHHH
GIAPFMADIFPNLTLPTGLNVNHLSHDKAVVDAYVKDPLVHGMASTYLGNMLLNSEEPIL
CHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
ANAGKIKIPIYIFHGKEDQIADSAGSEVFFEVVGSSDKTLKIYEGLYHETMNERIEDRTK
ECCCEEEEEEEEEECCCCCHHHCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHH
VLTDLKKWFESHVN
HHHHHHHHHHHHCC
>Mature Secondary Structure
MNFFQMKFFSLFKGSFVFITRLAMSWINFYNLEDDIFVGSKGTKIFYRTYQPKEGRKGNR
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEECCCCCCCCCE
VLVVQHGIGEHSGRYEFLVEAFAGTGTASYLIDSHGHGRSEGKRGAVDSFSDYLSDLDKL
EEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
IEIAKKKEKVSKVTLLGHSMGAAISTFYAEEGTNQGNLNALIISALPIKVKLDLVMKLKK
HHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCEEEHHHHHHHHHH
GIAPFMADIFPNLTLPTGLNVNHLSHDKAVVDAYVKDPLVHGMASTYLGNMLLNSEEPIL
CHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
ANAGKIKIPIYIFHGKEDQIADSAGSEVFFEVVGSSDKTLKIYEGLYHETMNERIEDRTK
ECCCEEEEEEEEEECCCCCHHHCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHH
VLTDLKKWFESHVN
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-lysophosphatidylcholine; H2O

Specific reaction: 2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]