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The map label for this gene is mnmC

Identifier: 116329331

GI number: 116329331

Start: 3296428

End: 3298386

Strand: Direct

Name: mnmC

Synonym: LBL_2779

Alternate gene names: 116329331

Gene position: 3296428-3298386 (Clockwise)

Preceding gene: 116329330

Following gene: 116329332

Centisome position: 91.2

GC content: 43.54

Gene sequence:

>1959_bases
ATGCTCTCCTGGAAAAACGATCTCACTCCCGTATCGGATCGATTCGACGACATTTACTTTTCCCCCGAAAACGGCTTAGA
GGAAACCAGACACGTTTTTATCGGAGGAAACGACCTTCCCGACCGTTGGAGAAATTCGAATATTCAAAATCCATTTTGTA
TCTTGGAACTAGGCTTCGGAACCGGGCTTAATTTTTTTGCGACTTGGAAAGAATATCTAAAACAAGACAACCGGTTTAGA
CTTCATTTTATATCGGTTGAAAAATTCCCTCTCAGTCGGAAAGAAATCTCAAAAGCCGTTTCCGCGTTTCCCGAATTGGA
GGAAATCAAAGAGGAATTTTTATCCTCTTACCAAGATCTTATCCCGGGAATGAACTACTTCCGATTTTTAGAAGGGAAAA
TCCACCTAACCCTCTTTTTAGGAGACGTCTCTGACGCGTTATGTGAAATTTCTGGAAAAGCGGACGCTATTTTTTTGGAC
GGTTTCGCCCCTTCTAAAAATCCGGAGATGTGGGAAGAATCGGTTCTGGGAAACTTAAAAAACGTCTCCAAGTTCGGAAC
TACGTTTTCCACTTTTACCGTCGCACGAACAGTCCGAAATTCTTTATCCTACGCCGGTTTTACGCTGGAAAAACGTCCCG
GTTTCGGAAAGAAAAGGGAAATGTTGACCGGAAAATATTCCAATTACCCTTCCGAAACGAAAGAATCGCTTCCGAAAGAA
AAACCTTGGTGCAAACGTTCCGATCCCGAATCACAAATCAAAACCGCGACGATCATTGGAGCGGGGATCGCAGGTTCGAC
GCTTGCTTATTCCTTGTCAAAACGAGGAATTCAAGTTTTCCTAATCGACCCGTCCGGAATCGCAAACGAAACCTCCGGAA
TTCCGAGAGCAATTTCCCATCCTCATCTCACGAAAATTCCAGGACCGATCAGTTTATTCACGTTACGCGCATTTCGATAT
GCGCTTTCCTTTCTTTCCTCATTTGCCGACAAAGATCAATTTGGAAAAGTCGGATCGTTTCACGGCGTAACGGCGGAGAT
GAATCCCGAAAGATTCCGAAAGAGTATAGAAAATCATAAGCTTACCGAAGAAATCGCCTCCTGGAAACCGAACGGCTCCG
ATTTCCACAAAAACGACCCATTCAACAAAGAATTAGAAGAAGGAGTCTTGTTCCGAAACGGTTTCTGGACCCGACCCGGA
TCTATCGCAAGAAAATGCGTCGATCAGCCTGGAATCGAATTGATACAAGCTACGGCAAACTCCTTCGAACGGATCGGATC
TTCCTGGAAACTCGACTTGAAAGAATCGGACCAAAAAGTACTTGCAGACTCTATTATATTTTGTAATTCTTATTTGATTG
GAAAACTAGCCTCGTCTCTGTTTGAAGGAGAAGAGATATTTCCGATAAACAAAGTCCGGGGACAACTTATATCCTTGAAA
GAAACGGAAAATTCCTCTCGTGTTCCGAACATTCTTTGTGCGGAACACTATTTAACGCCTTCCGTTCAAGGAGAACACGT
TTTAGGTTCCACGTTCGATGAATTCGACTTAAATCCGAAGCCCCGAAAAAAGGATACGGATTTACTTTTGCAATACGTTC
AAACCAAATATCCTACTCTAAGATTCGATTCGAACTGCGTTCTCTCCGAAAAAACCGGATTCCGAGCTCAAACTCCGGAT
CGGTTCCCGATCATTGGTCCGATCTTTGATCCGAAAATATTTCAGGAAACTTATAAAGAAATCGATTTACCTCGAAATCG
GAACAAAAAGTTTCCAAACCTCAAGGTGATTCCGGGCTTATACGTTTTCGGAGGTTTGGGTTCGAGGGGAATTTTGAGTT
CTTTTTTAGGTGCGGAGATTCTTGCGTCTTTAATACTGGGAGAACCCGCTCCCGTCGAATTCTCTCTCTTGGAATCGTTG
CATCCGGCTCGATTTCTCTATCGAAGAATCCGTAAGTAG

Upstream 100 bases:

>100_bases
TATCCAGATTGACATCGGATGATTTACTACGTAAAGAAAGTGGTCCCGGTATAGAGTCCGCTCCTCCAAAACCAACAGAA
CCCGTACCCCCGAAAATCTA

Downstream 100 bases:

>100_bases
GATGAGTTCAATATAAAAAACGGGAAAGCGATCGTAGTTCAAATGTTTGAATCGATTTTCCTCTCTTCTTAACTCTAAAA
AAAATTCAACACGGAGCTTG

Product: bifunctional hypothetical protein/glycine D-amino acid oxidase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MLSWKNDLTPVSDRFDDIYFSPENGLEETRHVFIGGNDLPDRWRNSNIQNPFCILELGFGTGLNFFATWKEYLKQDNRFR
LHFISVEKFPLSRKEISKAVSAFPELEEIKEEFLSSYQDLIPGMNYFRFLEGKIHLTLFLGDVSDALCEISGKADAIFLD
GFAPSKNPEMWEESVLGNLKNVSKFGTTFSTFTVARTVRNSLSYAGFTLEKRPGFGKKREMLTGKYSNYPSETKESLPKE
KPWCKRSDPESQIKTATIIGAGIAGSTLAYSLSKRGIQVFLIDPSGIANETSGIPRAISHPHLTKIPGPISLFTLRAFRY
ALSFLSSFADKDQFGKVGSFHGVTAEMNPERFRKSIENHKLTEEIASWKPNGSDFHKNDPFNKELEEGVLFRNGFWTRPG
SIARKCVDQPGIELIQATANSFERIGSSWKLDLKESDQKVLADSIIFCNSYLIGKLASSLFEGEEIFPINKVRGQLISLK
ETENSSRVPNILCAEHYLTPSVQGEHVLGSTFDEFDLNPKPRKKDTDLLLQYVQTKYPTLRFDSNCVLSEKTGFRAQTPD
RFPIIGPIFDPKIFQETYKEIDLPRNRNKKFPNLKVIPGLYVFGGLGSRGILSSFLGAEILASLILGEPAPVEFSLLESL
HPARFLYRRIRK

Sequences:

>Translated_652_residues
MLSWKNDLTPVSDRFDDIYFSPENGLEETRHVFIGGNDLPDRWRNSNIQNPFCILELGFGTGLNFFATWKEYLKQDNRFR
LHFISVEKFPLSRKEISKAVSAFPELEEIKEEFLSSYQDLIPGMNYFRFLEGKIHLTLFLGDVSDALCEISGKADAIFLD
GFAPSKNPEMWEESVLGNLKNVSKFGTTFSTFTVARTVRNSLSYAGFTLEKRPGFGKKREMLTGKYSNYPSETKESLPKE
KPWCKRSDPESQIKTATIIGAGIAGSTLAYSLSKRGIQVFLIDPSGIANETSGIPRAISHPHLTKIPGPISLFTLRAFRY
ALSFLSSFADKDQFGKVGSFHGVTAEMNPERFRKSIENHKLTEEIASWKPNGSDFHKNDPFNKELEEGVLFRNGFWTRPG
SIARKCVDQPGIELIQATANSFERIGSSWKLDLKESDQKVLADSIIFCNSYLIGKLASSLFEGEEIFPINKVRGQLISLK
ETENSSRVPNILCAEHYLTPSVQGEHVLGSTFDEFDLNPKPRKKDTDLLLQYVQTKYPTLRFDSNCVLSEKTGFRAQTPD
RFPIIGPIFDPKIFQETYKEIDLPRNRNKKFPNLKVIPGLYVFGGLGSRGILSSFLGAEILASLILGEPAPVEFSLLESL
HPARFLYRRIRK
>Mature_652_residues
MLSWKNDLTPVSDRFDDIYFSPENGLEETRHVFIGGNDLPDRWRNSNIQNPFCILELGFGTGLNFFATWKEYLKQDNRFR
LHFISVEKFPLSRKEISKAVSAFPELEEIKEEFLSSYQDLIPGMNYFRFLEGKIHLTLFLGDVSDALCEISGKADAIFLD
GFAPSKNPEMWEESVLGNLKNVSKFGTTFSTFTVARTVRNSLSYAGFTLEKRPGFGKKREMLTGKYSNYPSETKESLPKE
KPWCKRSDPESQIKTATIIGAGIAGSTLAYSLSKRGIQVFLIDPSGIANETSGIPRAISHPHLTKIPGPISLFTLRAFRY
ALSFLSSFADKDQFGKVGSFHGVTAEMNPERFRKSIENHKLTEEIASWKPNGSDFHKNDPFNKELEEGVLFRNGFWTRPG
SIARKCVDQPGIELIQATANSFERIGSSWKLDLKESDQKVLADSIIFCNSYLIGKLASSLFEGEEIFPINKVRGQLISLK
ETENSSRVPNILCAEHYLTPSVQGEHVLGSTFDEFDLNPKPRKKDTDLLLQYVQTKYPTLRFDSNCVLSEKTGFRAQTPD
RFPIIGPIFDPKIFQETYKEIDLPRNRNKKFPNLKVIPGLYVFGGLGSRGILSSFLGAEILASLILGEPAPVEFSLLESL
HPARFLYRRIRK

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=676, Percent_Identity=31.508875739645, Blast_Score=291, Evalue=7e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_LEPBJ (Q04VP5)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_799783.1
- ProteinModelPortal:   Q04VP5
- SMR:   Q04VP5
- STRING:   Q04VP5
- GeneID:   4409864
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_0297
- eggNOG:   COG0665
- HOGENOM:   HBG110595
- OMA:   VIGETGF
- PhylomeDB:   Q04VP5
- ProtClustDB:   CLSK574883
- BioCyc:   LBOR355277:LBJ_0297-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 73703; Mature: 73703

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSWKNDLTPVSDRFDDIYFSPENGLEETRHVFIGGNDLPDRWRNSNIQNPFCILELGFG
CCCCCCCCCCHHHCCCCEEECCCCCCCCCCEEEECCCCCCHHHCCCCCCCCEEEEEECCC
TGLNFFATWKEYLKQDNRFRLHFISVEKFPLSRKEISKAVSAFPELEEIKEEFLSSYQDL
CCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IPGMNYFRFLEGKIHLTLFLGDVSDALCEISGKADAIFLDGFAPSKNPEMWEESVLGNLK
HCCCHHHHHCCCEEEEEEEECCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHH
NVSKFGTTFSTFTVARTVRNSLSYAGFTLEKRPGFGKKREMLTGKYSNYPSETKESLPKE
HHHHHCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHCCCCCCCCCHHHHCCCCC
KPWCKRSDPESQIKTATIIGAGIAGSTLAYSLSKRGIQVFLIDPSGIANETSGIPRAISH
CCCCCCCCCHHHHEEEEEEECCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCC
PHLTKIPGPISLFTLRAFRYALSFLSSFADKDQFGKVGSFHGVTAEMNPERFRKSIENHK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
LTEEIASWKPNGSDFHKNDPFNKELEEGVLFRNGFWTRPGSIARKCVDQPGIELIQATAN
HHHHHHCCCCCCCCCCCCCCCCHHHHHCEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHH
SFERIGSSWKLDLKESDQKVLADSIIFCNSYLIGKLASSLFEGEEIFPINKVRGQLISLK
HHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEE
ETENSSRVPNILCAEHYLTPSVQGEHVLGSTFDEFDLNPKPRKKDTDLLLQYVQTKYPTL
ECCCCCCCCCEEEHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHCCCEE
RFDSNCVLSEKTGFRAQTPDRFPIIGPIFDPKIFQETYKEIDLPRNRNKKFPNLKVIPGL
EECCCEEEECCCCCCCCCCCCCCEECCCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCE
YVFGGLGSRGILSSFLGAEILASLILGEPAPVEFSLLESLHPARFLYRRIRK
EEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MLSWKNDLTPVSDRFDDIYFSPENGLEETRHVFIGGNDLPDRWRNSNIQNPFCILELGFG
CCCCCCCCCCHHHCCCCEEECCCCCCCCCCEEEECCCCCCHHHCCCCCCCCEEEEEECCC
TGLNFFATWKEYLKQDNRFRLHFISVEKFPLSRKEISKAVSAFPELEEIKEEFLSSYQDL
CCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IPGMNYFRFLEGKIHLTLFLGDVSDALCEISGKADAIFLDGFAPSKNPEMWEESVLGNLK
HCCCHHHHHCCCEEEEEEEECCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHH
NVSKFGTTFSTFTVARTVRNSLSYAGFTLEKRPGFGKKREMLTGKYSNYPSETKESLPKE
HHHHHCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHCCCCCCCCCHHHHCCCCC
KPWCKRSDPESQIKTATIIGAGIAGSTLAYSLSKRGIQVFLIDPSGIANETSGIPRAISH
CCCCCCCCCHHHHEEEEEEECCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCC
PHLTKIPGPISLFTLRAFRYALSFLSSFADKDQFGKVGSFHGVTAEMNPERFRKSIENHK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
LTEEIASWKPNGSDFHKNDPFNKELEEGVLFRNGFWTRPGSIARKCVDQPGIELIQATAN
HHHHHHCCCCCCCCCCCCCCCCHHHHHCEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHH
SFERIGSSWKLDLKESDQKVLADSIIFCNSYLIGKLASSLFEGEEIFPINKVRGQLISLK
HHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEE
ETENSSRVPNILCAEHYLTPSVQGEHVLGSTFDEFDLNPKPRKKDTDLLLQYVQTKYPTL
ECCCCCCCCCEEEHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHCCCEE
RFDSNCVLSEKTGFRAQTPDRFPIIGPIFDPKIFQETYKEIDLPRNRNKKFPNLKVIPGL
EECCCEEEECCCCCCCCCCCCCCEECCCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCE
YVFGGLGSRGILSSFLGAEILASLILGEPAPVEFSLLESLHPARFLYRRIRK
EEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA