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The map label for this gene is ygiD [H]

Identifier: 116329298

GI number: 116329298

Start: 3247486

End: 3248259

Strand: Direct

Name: ygiD [H]

Synonym: LBL_2744

Alternate gene names: 116329298

Gene position: 3247486-3248259 (Clockwise)

Preceding gene: 116329291

Following gene: 116329303

Centisome position: 89.85

GC content: 43.93

Gene sequence:

>774_bases
ATGACAAATCCAGTTTTATTTTTTGGACACGGCTCTCCGATGAACCTCGTAACTCCGTCCGACTTCACTCGGAACCTGAA
GGAATTTGGTGCGACACTTCAGGCGGTGAAAAATATACTCGTCGTTTCCGCGCATTGGAAAACGAGAGGAACTTACGTGA
CCACCTCTGATCCACCGAAACAGATTTATGATTTTTACGGATTTCCTCCCGAATTATACGAGATTCAATATCGACCTTCC
GGATCCCCTGAGCTCGCCGAGAAGATTCAGAATTCGCTCAAAACCGTAAACGCTCAACCTACACAAGAATGGGGACTCGA
TCATGGGAGTTGGGGAGTATTGTGTTTCCTATTTCAAAAAGCAAATATCCCCGTACTTCAATTGAGCCTCGATACGAACT
TAAGCCCCGAAAAACAATACGAGATCGGAAAAGAATTACGACCACTCCGAGAGGAGGGAACCTTAATCTTAGGAAGCGGA
AACATCGTTCACAACCTGCAAAAAGCCGATTTTTACGATATGAATGCGACACCTACGGATTGGGCGATCGAGTTCGATGA
GTTCATACGACAAATGCTGGAATCCAGAAACGATAAAGAGATTTTAGACTTTCAAAAAAAAGGAGACATCGCAACGCTCT
CGGTGCCTACCACGGAACATTTGGAACCGATTTTTTACGTTTTAGGCGCGATGAAACCCGAAGAGAAAGTGAAGTTCATT
CATCATAGTTTTCAGAATCGAACCGTATCCATGCAATCTTTTACTTCGGTTTAA

Upstream 100 bases:

>100_bases
TAACTCTCCGTTCGTTTCCGTTTATAAAACGGTCGATTGAAGCGGTTTTGTTCATTTGAACTTTTTCAACAACTCTAATA
AGGAAAGGAAATTTCTTTTT

Downstream 100 bases:

>100_bases
AAAAATGCGAGTTCGACGTAAGACGGTCCGTTTCTGTTTTATGAAAACGGTCGATTGAAGCAGTTTCGTTTATCCGAACT
ATGGAATTTTTCAACAACTC

Product: catalytic LigB subunit of aromatic ring-opening dioxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTNPVLFFGHGSPMNLVTPSDFTRNLKEFGATLQAVKNILVVSAHWKTRGTYVTTSDPPKQIYDFYGFPPELYEIQYRPS
GSPELAEKIQNSLKTVNAQPTQEWGLDHGSWGVLCFLFQKANIPVLQLSLDTNLSPEKQYEIGKELRPLREEGTLILGSG
NIVHNLQKADFYDMNATPTDWAIEFDEFIRQMLESRNDKEILDFQKKGDIATLSVPTTEHLEPIFYVLGAMKPEEKVKFI
HHSFQNRTVSMQSFTSV

Sequences:

>Translated_257_residues
MTNPVLFFGHGSPMNLVTPSDFTRNLKEFGATLQAVKNILVVSAHWKTRGTYVTTSDPPKQIYDFYGFPPELYEIQYRPS
GSPELAEKIQNSLKTVNAQPTQEWGLDHGSWGVLCFLFQKANIPVLQLSLDTNLSPEKQYEIGKELRPLREEGTLILGSG
NIVHNLQKADFYDMNATPTDWAIEFDEFIRQMLESRNDKEILDFQKKGDIATLSVPTTEHLEPIFYVLGAMKPEEKVKFI
HHSFQNRTVSMQSFTSV
>Mature_256_residues
TNPVLFFGHGSPMNLVTPSDFTRNLKEFGATLQAVKNILVVSAHWKTRGTYVTTSDPPKQIYDFYGFPPELYEIQYRPSG
SPELAEKIQNSLKTVNAQPTQEWGLDHGSWGVLCFLFQKANIPVLQLSLDTNLSPEKQYEIGKELRPLREEGTLILGSGN
IVHNLQKADFYDMNATPTDWAIEFDEFIRQMLESRNDKEILDFQKKGDIATLSVPTTEHLEPIFYVLGAMKPEEKVKFIH
HSFQNRTVSMQSFTSV

Specific function: Unknown

COG id: COG3384

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DODA-type extradiol aromatic ring- opening dioxygenase family [H]

Homologues:

Organism=Escherichia coli, GI226510974, Length=254, Percent_Identity=39.3700787401575, Blast_Score=192, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014436
- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: NA

Molecular weight: Translated: 29276; Mature: 29144

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPVLFFGHGSPMNLVTPSDFTRNLKEFGATLQAVKNILVVSAHWKTRGTYVTTSDPPK
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEECCCCHH
QIYDFYGFPPELYEIQYRPSGSPELAEKIQNSLKTVNAQPTQEWGLDHGSWGVLCFLFQK
HHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHEEEEEEEC
ANIPVLQLSLDTNLSPEKQYEIGKELRPLREEGTLILGSGNIVHNLQKADFYDMNATPTD
CCCCEEEEEECCCCCCHHHHHHHHHHCCHHHCCCEEEECCHHHHHHHHCCEEECCCCCCC
WAIEFDEFIRQMLESRNDKEILDFQKKGDIATLSVPTTEHLEPIFYVLGAMKPEEKVKFI
CEECHHHHHHHHHHCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHH
HHSFQNRTVSMQSFTSV
HHHHCCCEEEHHHHCCC
>Mature Secondary Structure 
TNPVLFFGHGSPMNLVTPSDFTRNLKEFGATLQAVKNILVVSAHWKTRGTYVTTSDPPK
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEECCCCHH
QIYDFYGFPPELYEIQYRPSGSPELAEKIQNSLKTVNAQPTQEWGLDHGSWGVLCFLFQK
HHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHEEEEEEEC
ANIPVLQLSLDTNLSPEKQYEIGKELRPLREEGTLILGSGNIVHNLQKADFYDMNATPTD
CCCCEEEEEECCCCCCHHHHHHHHHHCCHHHCCCEEEECCHHHHHHHHCCEEECCCCCCC
WAIEFDEFIRQMLESRNDKEILDFQKKGDIATLSVPTTEHLEPIFYVLGAMKPEEKVKFI
CEECHHHHHHHHHHCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHH
HHSFQNRTVSMQSFTSV
HHHHCCCEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1314093; 9278503 [H]