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The map label for this gene is amtB [H]

Identifier: 116329266

GI number: 116329266

Start: 3205927

End: 3207210

Strand: Reverse

Name: amtB [H]

Synonym: LBL_2706

Alternate gene names: 116329266

Gene position: 3207210-3205927 (Counterclockwise)

Preceding gene: 116329267

Following gene: 116329265

Centisome position: 88.73

GC content: 43.61

Gene sequence:

>1284_bases
ATGATTATGAATTGGACTAAAAGGTTAATCTTATTACTTCTTCTACTTCTTCCATTTGTCGTATTTGGACAAGAAGCAAC
TGCTTCCACTGTAGCGGATAAAGGAGACACAGTCTGGATGATCGTCGCATCGGCTCTCGTGTTTTTTATGATTCCTGGAT
TAGCGTTGTTTTATGGCGGTCTTGTAAGATCTAAAAACGTTCTTTCGACTATGATGCATAGTTTTGTGGCGATCTTAGTT
TTAACTCTACAGTGGACAGTTTTTGGATATAGTTTTGCGTTTTCGGGAACTAATCCTTATTTTGGAAATTTTGATCTCGC
TTTCCTGAACGGAATTGATGAAAAAACGTTGGAATTGACGATTCCCAAATACATTCACTTCCTTTTTCAAGGAATGTTTG
CATTGATCACACCTGCTCTGATTTCGGGAGCGATTGCTGAAAGGGTAAAATTCGGCGGTTATATAGTCTTTATTTTTCTT
TGGTCCACTCTTGTTTATGATCCGGTTGCACACTGGGTTTGGTCGGCGGATGGTTGGCTCTTTAAGATGAGCGCTTTGGA
TTTCGCGGGAGGAACCGTAGTACACTTGATTTCCGGAATCGCGGGACTTGCGGCAGCGATTGTTCTCGGAAAAAGAAAAG
GAGAAGGACCTTCTCTGATCGCTCCGAACAATCTTACTTACACTTTAATCGGTGCCGGTTTACTTTGGTTCGGTTGGTTC
GGTTTTAACGCAGGTTCCGGGCTTGCGGTAAACGGAATCGCAGCAAGAGCATTCTTAGTTACATTGATTGCTCCTGCGGC
TGCCGGTGTTGCATGGCTTATAATCGAATATCTTCATACAAGAAAAGCTACCGCTCTTGGAGCCGCTTCCGGTATCGTCG
CAGGTTTAGTTGTGATTACTCCCGCATCCGGATTTGTCGGTGTTCAAGGCGCTATCATCATGGGTTTTCTCGTAAGTCCC
GTGTGTTACGGTGCGATTCTTTTGAAAGGAAAACTCGGATACGACGATAGCCTGGATGCTTTCGGAATTCACGGAGTAGG
AGGTGCGTTAGGCGCTATCCTTACTGGAATTTTCACTTTCACTCTTGGCGCGGGCGTTGCAAGTAGAGGAGATCAGATTC
TCGTTCAAATAATCAGTGTTATTGCAACCGGAGCGTATTCTTTTGTAGTGTCTGTGATTCTTGTATTTCTAATCGATAAA
ACGATCGGGTTCCGTATCTCGGAAGAAAAAGAAATCACCGGTTTGGATTCGGAGATCCACGGCGAAAAAGGTTATATTCT
TTAA

Upstream 100 bases:

>100_bases
CATTTCAAGAGCAGTCTTTTCCTATTCTGAAAATTCTTAAATTATTTTTTAGTTGGTATCGATTTTGCAAGCAATAGGTA
TCAATTGATTTGGGATAAGA

Downstream 100 bases:

>100_bases
TTAACTACTGAAGAATTTTGAATAAGGAGATTCTATGAAATTAATCGTTGCTATCATTCAGCCTCATAAATTGGAAGAGG
TAAAGGCGGAATTAACGAAG

Product: ammonia permease

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MIMNWTKRLILLLLLLLPFVVFGQEATASTVADKGDTVWMIVASALVFFMIPGLALFYGGLVRSKNVLSTMMHSFVAILV
LTLQWTVFGYSFAFSGTNPYFGNFDLAFLNGIDEKTLELTIPKYIHFLFQGMFALITPALISGAIAERVKFGGYIVFIFL
WSTLVYDPVAHWVWSADGWLFKMSALDFAGGTVVHLISGIAGLAAAIVLGKRKGEGPSLIAPNNLTYTLIGAGLLWFGWF
GFNAGSGLAVNGIAARAFLVTLIAPAAAGVAWLIIEYLHTRKATALGAASGIVAGLVVITPASGFVGVQGAIIMGFLVSP
VCYGAILLKGKLGYDDSLDAFGIHGVGGALGAILTGIFTFTLGAGVASRGDQILVQIISVIATGAYSFVVSVILVFLIDK
TIGFRISEEKEITGLDSEIHGEKGYIL

Sequences:

>Translated_427_residues
MIMNWTKRLILLLLLLLPFVVFGQEATASTVADKGDTVWMIVASALVFFMIPGLALFYGGLVRSKNVLSTMMHSFVAILV
LTLQWTVFGYSFAFSGTNPYFGNFDLAFLNGIDEKTLELTIPKYIHFLFQGMFALITPALISGAIAERVKFGGYIVFIFL
WSTLVYDPVAHWVWSADGWLFKMSALDFAGGTVVHLISGIAGLAAAIVLGKRKGEGPSLIAPNNLTYTLIGAGLLWFGWF
GFNAGSGLAVNGIAARAFLVTLIAPAAAGVAWLIIEYLHTRKATALGAASGIVAGLVVITPASGFVGVQGAIIMGFLVSP
VCYGAILLKGKLGYDDSLDAFGIHGVGGALGAILTGIFTFTLGAGVASRGDQILVQIISVIATGAYSFVVSVILVFLIDK
TIGFRISEEKEITGLDSEIHGEKGYIL
>Mature_427_residues
MIMNWTKRLILLLLLLLPFVVFGQEATASTVADKGDTVWMIVASALVFFMIPGLALFYGGLVRSKNVLSTMMHSFVAILV
LTLQWTVFGYSFAFSGTNPYFGNFDLAFLNGIDEKTLELTIPKYIHFLFQGMFALITPALISGAIAERVKFGGYIVFIFL
WSTLVYDPVAHWVWSADGWLFKMSALDFAGGTVVHLISGIAGLAAAIVLGKRKGEGPSLIAPNNLTYTLIGAGLLWFGWF
GFNAGSGLAVNGIAARAFLVTLIAPAAAGVAWLIIEYLHTRKATALGAASGIVAGLVVITPASGFVGVQGAIIMGFLVSP
VCYGAILLKGKLGYDDSLDAFGIHGVGGALGAILTGIFTFTLGAGVASRGDQILVQIISVIATGAYSFVVSVILVFLIDK
TIGFRISEEKEITGLDSEIHGEKGYIL

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=419, Percent_Identity=45.8233890214797, Blast_Score=325, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI32564042, Length=414, Percent_Identity=30.1932367149758, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI133931100, Length=441, Percent_Identity=26.984126984127, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17550180, Length=421, Percent_Identity=28.2660332541568, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17550182, Length=433, Percent_Identity=29.0993071593533, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6325396, Length=410, Percent_Identity=36.3414634146341, Blast_Score=260, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6324187, Length=421, Percent_Identity=37.7672209026128, Blast_Score=257, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6321559, Length=415, Percent_Identity=35.421686746988, Blast_Score=242, Evalue=8e-65,
Organism=Drosophila melanogaster, GI161078314, Length=406, Percent_Identity=30.2955665024631, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45329; Mature: 45329

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMNWTKRLILLLLLLLPFVVFGQEATASTVADKGDTVWMIVASALVFFMIPGLALFYGG
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LVRSKNVLSTMMHSFVAILVLTLQWTVFGYSFAFSGTNPYFGNFDLAFLNGIDEKTLELT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEECCCCCCCEEEE
IPKYIHFLFQGMFALITPALISGAIAERVKFGGYIVFIFLWSTLVYDPVAHWVWSADGWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCE
FKMSALDFAGGTVVHLISGIAGLAAAIVLGKRKGEGPSLIAPNNLTYTLIGAGLLWFGWF
EEEEHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEHHHHHHHHHHHHH
GFNAGSGLAVNGIAARAFLVTLIAPAAAGVAWLIIEYLHTRKATALGAASGIVAGLVVIT
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
PASGFVGVQGAIIMGFLVSPVCYGAILLKGKLGYDDSLDAFGIHGVGGALGAILTGIFTF
CCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TLGAGVASRGDQILVQIISVIATGAYSFVVSVILVFLIDKTIGFRISEEKEITGLDSEIH
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCHHC
GEKGYIL
CCCCCCC
>Mature Secondary Structure
MIMNWTKRLILLLLLLLPFVVFGQEATASTVADKGDTVWMIVASALVFFMIPGLALFYGG
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LVRSKNVLSTMMHSFVAILVLTLQWTVFGYSFAFSGTNPYFGNFDLAFLNGIDEKTLELT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEECCCCCCCEEEE
IPKYIHFLFQGMFALITPALISGAIAERVKFGGYIVFIFLWSTLVYDPVAHWVWSADGWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCE
FKMSALDFAGGTVVHLISGIAGLAAAIVLGKRKGEGPSLIAPNNLTYTLIGAGLLWFGWF
EEEEHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEHHHHHHHHHHHHH
GFNAGSGLAVNGIAARAFLVTLIAPAAAGVAWLIIEYLHTRKATALGAASGIVAGLVVIT
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
PASGFVGVQGAIIMGFLVSPVCYGAILLKGKLGYDDSLDAFGIHGVGGALGAILTGIFTF
CCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TLGAGVASRGDQILVQIISVIATGAYSFVVSVILVFLIDKTIGFRISEEKEITGLDSEIH
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCHHC
GEKGYIL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]