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The map label for this gene is hemK [H]

Identifier: 116329264

GI number: 116329264

Start: 3203951

End: 3204811

Strand: Direct

Name: hemK [H]

Synonym: LBL_2704

Alternate gene names: 116329264

Gene position: 3203951-3204811 (Clockwise)

Preceding gene: 116329263

Following gene: 116329271

Centisome position: 88.64

GC content: 37.75

Gene sequence:

>861_bases
ATGCAACATCCAGATTCAATTCTCACTCTTTTGAAAAAATCAGAGGAATTCTTGAGAAAGAAAGAAATTCCGAGCGCCAG
ATTAGATGCGGAAATCCTTTTAGCCGACCTCCTCAATCTCCAAAGAGTAAAGTTATACGTGAACTTTGAAAGACTCCTAA
CAGAAACGGAAAAAAACGCTTACAGAGAAAGAATCGTAGACCGTTCCAAAAACAAACCCACAGCTTATATTACGGGCCAA
AAGGCTTTTTACAATTCTGTTTTCTTCGTAAACGAGAAAGTCCTCATACCGAGACCCGAAACCGAAGAGCTCGTGGAAAA
GATTTTACTCGACTTCAAAGGTAATAATAGCGAACAAAACGTATTGGATCTTTGTACGGGAAGCGGTTGTATTGGAATTA
GCTTGAAATCAGCGAGGAAAGATTGGAATATTACATTAAGTGATATTTCAAAAGACGCTCTTGAAATCGCGAAAAAAAAT
GCGATCCAAATTATCGGAGAAGGAAATAATATTCAATTTTTGGAAAGTAATTTATTTCTTTCGATCCCGAAAGAATCCGA
ATTCGATTTGATCGTCACAAATCCTCCCTATATTCCCATTTCAGATAAAACAGAAATGATGAAGGATGTGGTCGACTACG
AACCTCATCTAGCGCTATTTTTGGAAGACCCAAAGGAATTCTTATCCAAATTGATCGAAGACGCCCGAATTCATCTGAAA
GAAGGTGGAAAGTTTTATATGGAAACTTATCCTTCCTTGGCTTGGACTTTCGTTTCCGAATCGACAACAAATGGCTGGAA
AGAAGGAAAAGTAGAAAAGGATCTTTCTGGAAAAGATCGATTTGTCGTTTTGACAAAATAG

Upstream 100 bases:

>100_bases
TTCTCATAATTCATCAAAATCTAGAATCGTACTTAAAAAAATGGAATCCACTGCAATGGAATTCCTTAACTTGACGACAC
TAAACCCGAATTTCAAACCG

Downstream 100 bases:

>100_bases
TCGAAGAATGTTTTCCTTTTGAATCGGAAACCTCCTAAAGACGCTTAGGTTCGTTTTTCCAAAAAAAATCCTAAATTTTT
GAAAACGTACGGGAAATTTC

Product: methylase of polypeptide chain release factors

Products: NA

Alternate protein names: HemK protein homolog; M.RcoHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MQHPDSILTLLKKSEEFLRKKEIPSARLDAEILLADLLNLQRVKLYVNFERLLTETEKNAYRERIVDRSKNKPTAYITGQ
KAFYNSVFFVNEKVLIPRPETEELVEKILLDFKGNNSEQNVLDLCTGSGCIGISLKSARKDWNITLSDISKDALEIAKKN
AIQIIGEGNNIQFLESNLFLSIPKESEFDLIVTNPPYIPISDKTEMMKDVVDYEPHLALFLEDPKEFLSKLIEDARIHLK
EGGKFYMETYPSLAWTFVSESTTNGWKEGKVEKDLSGKDRFVVLTK

Sequences:

>Translated_286_residues
MQHPDSILTLLKKSEEFLRKKEIPSARLDAEILLADLLNLQRVKLYVNFERLLTETEKNAYRERIVDRSKNKPTAYITGQ
KAFYNSVFFVNEKVLIPRPETEELVEKILLDFKGNNSEQNVLDLCTGSGCIGISLKSARKDWNITLSDISKDALEIAKKN
AIQIIGEGNNIQFLESNLFLSIPKESEFDLIVTNPPYIPISDKTEMMKDVVDYEPHLALFLEDPKEFLSKLIEDARIHLK
EGGKFYMETYPSLAWTFVSESTTNGWKEGKVEKDLSGKDRFVVLTK
>Mature_286_residues
MQHPDSILTLLKKSEEFLRKKEIPSARLDAEILLADLLNLQRVKLYVNFERLLTETEKNAYRERIVDRSKNKPTAYITGQ
KAFYNSVFFVNEKVLIPRPETEELVEKILLDFKGNNSEQNVLDLCTGSGCIGISLKSARKDWNITLSDISKDALEIAKKN
AIQIIGEGNNIQFLESNLFLSIPKESEFDLIVTNPPYIPISDKTEMMKDVVDYEPHLALFLEDPKEFLSKLIEDARIHLK
EGGKFYMETYPSLAWTFVSESTTNGWKEGKVEKDLSGKDRFVVLTK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=305, Percent_Identity=25.5737704918033, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI87082085, Length=220, Percent_Identity=34.0909090909091, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1787463, Length=262, Percent_Identity=31.6793893129771, Blast_Score=112, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324266, Length=209, Percent_Identity=29.6650717703349, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24582226, Length=282, Percent_Identity=25.886524822695, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 32785; Mature: 32785

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHPDSILTLLKKSEEFLRKKEIPSARLDAEILLADLLNLQRVKLYVNFERLLTETEKNA
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHH
YRERIVDRSKNKPTAYITGQKAFYNSVFFVNEKVLIPRPETEELVEKILLDFKGNNSEQN
HHHHHHHCCCCCCEEEEECCHHHHCEEEEEECEEEECCCCHHHHHHHHHHHCCCCCCCCH
VLDLCTGSGCIGISLKSARKDWNITLSDISKDALEIAKKNAIQIIGEGNNIQFLESNLFL
HHHHHCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCEEE
SIPKESEFDLIVTNPPYIPISDKTEMMKDVVDYEPHLALFLEDPKEFLSKLIEDARIHLK
ECCCCCCCCEEEECCCEEECCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHEEEEC
EGGKFYMETYPSLAWTFVSESTTNGWKEGKVEKDLSGKDRFVVLTK
CCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MQHPDSILTLLKKSEEFLRKKEIPSARLDAEILLADLLNLQRVKLYVNFERLLTETEKNA
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHH
YRERIVDRSKNKPTAYITGQKAFYNSVFFVNEKVLIPRPETEELVEKILLDFKGNNSEQN
HHHHHHHCCCCCCEEEEECCHHHHCEEEEEECEEEECCCCHHHHHHHHHHHCCCCCCCCH
VLDLCTGSGCIGISLKSARKDWNITLSDISKDALEIAKKNAIQIIGEGNNIQFLESNLFL
HHHHHCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCEEE
SIPKESEFDLIVTNPPYIPISDKTEMMKDVVDYEPHLALFLEDPKEFLSKLIEDARIHLK
ECCCCCCCCEEEECCCEEECCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHEEEEC
EGGKFYMETYPSLAWTFVSESTTNGWKEGKVEKDLSGKDRFVVLTK
CCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]