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The map label for this gene is thiC [H]

Identifier: 116329235

GI number: 116329235

Start: 3167584

End: 3169119

Strand: Reverse

Name: thiC [H]

Synonym: LBL_2674

Alternate gene names: 116329235

Gene position: 3169119-3167584 (Counterclockwise)

Preceding gene: 116329236

Following gene: 116329233

Centisome position: 87.68

GC content: 47.46

Gene sequence:

>1536_bases
ATGGAGTCCACTCAAGAATTTCAAGTCCCTCTGAAAACGATTCGTCTTACCGACGGGACCGAATATCAATCGTATCATAC
GGAGGGTTCGCTTTCTGACAAGAAACCTTCCGATTATAAAAACGGAATCCCAAAAATAAGGAAAGAATGGATCCGGACAC
GCCTCGATCGGGGTGATACGAATCATTCTCAGATGTATTATGCGAAAAAGGGAATCATCACCGAAGAGATGCGTTATGTG
GCCCTTCGGGAAAATATGAATCCCGAGTTCGTTCGTTTGGAAATCGCGTGCGGTCGCGCCATTCTTCCTTCCAATCGAAA
CCATCCCGAACTTGAACCGATGATCATTGGTAGGAATTTTCTCGTAAAGATCAATGCGAATATCGGAAACTCAGCGCTCG
GCTCCTCCATCGAAGAGGAAGTCGAAAAATTGCATTGGGCGGTCAAGTGGGGCGCGGACACGGTGATGGATCTTTCCACC
GGAAAAAACATCCACGAAACGAGAGAATGGATCCTCCGAAATTCTCCCGTGCCAATCGGAACGGTTCCGATCTATCAAGC
CCTTGAAAAGGTAAAAGGAAAAGCGGGGAATTTGAATATTCAAGTTTTCTTAGATACTTTGGAGGAACGAGCCGAACAAG
GAGTGGATTATTTTACGATTCATGCTGGAGTTCTTCTGCGTTATATTCCTTTTACCGCAAACCGCGTGACCGGGATCGTA
TCGAGAGGGGGATCGATTCTTGCAAAATGGTGTTTGGCGCATCATAAGGAGAATTTTCTTTATACGCATTTCGACGAAAT
TATAAAGGTGATGAAAAAATACGGTGTTTCCTTTTCTCTCGGAGACGGATTGCGTCCCGGTTCGATTGCGGATGCAAACG
ACAAGGCGCAATTCGGAGAACTCGAAACCTTGGGCGAATTGACAAAACGTGCCTGGGAAGAAGATATTCAGGTGATGATC
GAAGGACCGGGTCACGTTCCTATGCATCTCATCAAGGAGAATGTCGATCTTCAGATGAAACTCTGCCAAGAAGCCCCGTT
TTACACGTTAGGCCCTCTTGTTACCGATATCGCTCCGGGTTACGACCATATCACTTCCGCGATCGGTGCGGCGATGATCG
GATGGTTCGGAATGGCTATGCTCTGTTATGTGACTCCGAAAGAACACTTGGGACTTCCCGATAAGGAAGACGTCAAACAA
GGAGTGATTGCGTATAAGATCGCGGCTCATGCGGCCGATCTGGCAAAGGGACATCCGGGCGCGATCAAGCGGGACAATCT
TTTGAGCAAGGCCCGTTTCGAATTCCGTTGGGAGGATCAGTTCGCGCTTTCCCTCGATCCCGAAACCGCGAAGTCCTTTC
ATGACGAGACTCTTCCGCAGGATCGAATGAAAACCGCGCATTTCTGTTCTATGTGTGGTCCTCATTTTTGTTCGATGAAC
CTGACCCAAGAACTCCGAAAGTTTGCGCAGGAGAAGGGAATGGAAGAAAAGTCCGAGGTAACAATTTGTAATCGAAAAAA
AGAATCAGGAAAGTGA

Upstream 100 bases:

>100_bases
GGCCTTAGTCCGTGGTTTTTGAACGGGAGAATCTTCGGATCGTATCAGTTTTTTACAGCTTACCTCAGTTCCTTCTCCAA
ACCGACGGTCAGGAGAATGT

Downstream 100 bases:

>100_bases
TGAAATATTTTTTCATTAGAGTTGTTGAAAAATTCCATGATTCAGATTAATAAAATCGCTTCGAACGTCCATTTCAATGC
AGCAGAAGGATAAAGAATTA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MESTQEFQVPLKTIRLTDGTEYQSYHTEGSLSDKKPSDYKNGIPKIRKEWIRTRLDRGDTNHSQMYYAKKGIITEEMRYV
ALRENMNPEFVRLEIACGRAILPSNRNHPELEPMIIGRNFLVKINANIGNSALGSSIEEEVEKLHWAVKWGADTVMDLST
GKNIHETREWILRNSPVPIGTVPIYQALEKVKGKAGNLNIQVFLDTLEERAEQGVDYFTIHAGVLLRYIPFTANRVTGIV
SRGGSILAKWCLAHHKENFLYTHFDEIIKVMKKYGVSFSLGDGLRPGSIADANDKAQFGELETLGELTKRAWEEDIQVMI
EGPGHVPMHLIKENVDLQMKLCQEAPFYTLGPLVTDIAPGYDHITSAIGAAMIGWFGMAMLCYVTPKEHLGLPDKEDVKQ
GVIAYKIAAHAADLAKGHPGAIKRDNLLSKARFEFRWEDQFALSLDPETAKSFHDETLPQDRMKTAHFCSMCGPHFCSMN
LTQELRKFAQEKGMEEKSEVTICNRKKESGK

Sequences:

>Translated_511_residues
MESTQEFQVPLKTIRLTDGTEYQSYHTEGSLSDKKPSDYKNGIPKIRKEWIRTRLDRGDTNHSQMYYAKKGIITEEMRYV
ALRENMNPEFVRLEIACGRAILPSNRNHPELEPMIIGRNFLVKINANIGNSALGSSIEEEVEKLHWAVKWGADTVMDLST
GKNIHETREWILRNSPVPIGTVPIYQALEKVKGKAGNLNIQVFLDTLEERAEQGVDYFTIHAGVLLRYIPFTANRVTGIV
SRGGSILAKWCLAHHKENFLYTHFDEIIKVMKKYGVSFSLGDGLRPGSIADANDKAQFGELETLGELTKRAWEEDIQVMI
EGPGHVPMHLIKENVDLQMKLCQEAPFYTLGPLVTDIAPGYDHITSAIGAAMIGWFGMAMLCYVTPKEHLGLPDKEDVKQ
GVIAYKIAAHAADLAKGHPGAIKRDNLLSKARFEFRWEDQFALSLDPETAKSFHDETLPQDRMKTAHFCSMCGPHFCSMN
LTQELRKFAQEKGMEEKSEVTICNRKKESGK
>Mature_511_residues
MESTQEFQVPLKTIRLTDGTEYQSYHTEGSLSDKKPSDYKNGIPKIRKEWIRTRLDRGDTNHSQMYYAKKGIITEEMRYV
ALRENMNPEFVRLEIACGRAILPSNRNHPELEPMIIGRNFLVKINANIGNSALGSSIEEEVEKLHWAVKWGADTVMDLST
GKNIHETREWILRNSPVPIGTVPIYQALEKVKGKAGNLNIQVFLDTLEERAEQGVDYFTIHAGVLLRYIPFTANRVTGIV
SRGGSILAKWCLAHHKENFLYTHFDEIIKVMKKYGVSFSLGDGLRPGSIADANDKAQFGELETLGELTKRAWEEDIQVMI
EGPGHVPMHLIKENVDLQMKLCQEAPFYTLGPLVTDIAPGYDHITSAIGAAMIGWFGMAMLCYVTPKEHLGLPDKEDVKQ
GVIAYKIAAHAADLAKGHPGAIKRDNLLSKARFEFRWEDQFALSLDPETAKSFHDETLPQDRMKTAHFCSMCGPHFCSMN
LTQELRKFAQEKGMEEKSEVTICNRKKESGK

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=468, Percent_Identity=70.2991452991453, Blast_Score=690, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 57699; Mature: 57699

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESTQEFQVPLKTIRLTDGTEYQSYHTEGSLSDKKPSDYKNGIPKIRKEWIRTRLDRGDT
CCCCHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCC
NHSQMYYAKKGIITEEMRYVALRENMNPEFVRLEIACGRAILPSNRNHPELEPMIIGRNF
CCCEEEEHHCCCHHHHHHHHHHHCCCCCCEEEEEEECCCEECCCCCCCCCCCEEEECCEE
LVKINANIGNSALGSSIEEEVEKLHWAVKWGADTVMDLSTGKNIHETREWILRNSPVPIG
EEEEECCCCCHHHCCHHHHHHHHHHHHHEECCCCEEECCCCCCHHHHHHHHHCCCCCCCC
TVPIYQALEKVKGKAGNLNIQVFLDTLEERAEQGVDYFTIHAGVLLRYIPFTANRVTGIV
CCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCCCEEEEHHHHHHHHCCCCHHHHHHHH
SRGGSILAKWCLAHHKENFLYTHFDEIIKVMKKYGVSFSLGDGLRPGSIADANDKAQFGE
HCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCH
LETLGELTKRAWEEDIQVMIEGPGHVPMHLIKENVDLQMKLCQEAPFYTLGPLVTDIAPG
HHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHCCCCHHHHHHHCCCCEEHHHHHHHCCCC
YDHITSAIGAAMIGWFGMAMLCYVTPKEHLGLPDKEDVKQGVIAYKIAAHAADLAKGHPG
HHHHHHHHHHHHHHHHHHHHHHEECCHHHCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCC
AIKRDNLLSKARFEFRWEDQFALSLDPETAKSFHDETLPQDRMKTAHFCSMCGPHFCSMN
CCCHHHHHHHHHEEEEECCCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCC
LTQELRKFAQEKGMEEKSEVTICNRKKESGK
HHHHHHHHHHHCCCCCHHCEEEECCCCCCCC
>Mature Secondary Structure
MESTQEFQVPLKTIRLTDGTEYQSYHTEGSLSDKKPSDYKNGIPKIRKEWIRTRLDRGDT
CCCCHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCC
NHSQMYYAKKGIITEEMRYVALRENMNPEFVRLEIACGRAILPSNRNHPELEPMIIGRNF
CCCEEEEHHCCCHHHHHHHHHHHCCCCCCEEEEEEECCCEECCCCCCCCCCCEEEECCEE
LVKINANIGNSALGSSIEEEVEKLHWAVKWGADTVMDLSTGKNIHETREWILRNSPVPIG
EEEEECCCCCHHHCCHHHHHHHHHHHHHEECCCCEEECCCCCCHHHHHHHHHCCCCCCCC
TVPIYQALEKVKGKAGNLNIQVFLDTLEERAEQGVDYFTIHAGVLLRYIPFTANRVTGIV
CCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCCCEEEEHHHHHHHHCCCCHHHHHHHH
SRGGSILAKWCLAHHKENFLYTHFDEIIKVMKKYGVSFSLGDGLRPGSIADANDKAQFGE
HCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCH
LETLGELTKRAWEEDIQVMIEGPGHVPMHLIKENVDLQMKLCQEAPFYTLGPLVTDIAPG
HHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHCCCCHHHHHHHCCCCEEHHHHHHHCCCC
YDHITSAIGAAMIGWFGMAMLCYVTPKEHLGLPDKEDVKQGVIAYKIAAHAADLAKGHPG
HHHHHHHHHHHHHHHHHHHHHHEECCHHHCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCC
AIKRDNLLSKARFEFRWEDQFALSLDPETAKSFHDETLPQDRMKTAHFCSMCGPHFCSMN
CCCHHHHHHHHHEEEEECCCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCC
LTQELRKFAQEKGMEEKSEVTICNRKKESGK
HHHHHHHHHHHCCCCCHHCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA