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The map label for this gene is thiM

Identifier: 116329130

GI number: 116329130

Start: 3029975

End: 3030769

Strand: Direct

Name: thiM

Synonym: LBL_2553

Alternate gene names: 116329130

Gene position: 3029975-3030769 (Clockwise)

Preceding gene: 116329129

Following gene: 116329131

Centisome position: 83.83

GC content: 47.17

Gene sequence:

>795_bases
ATGCCAAAACATTCAGCCTTAGAAAGGGCCTGGCCCGCAAAAGAGATCGTAGAAGATCTTTTAGAGCTAAGAAAACGTTC
CCCTCTAACACATATCATGACAAACATCGTAGTCACCAATTGGACAGCAAACGTATTGCTTGCAGTCGGCGCCTCTCCCG
CGATGGTAATCGCCGAAGAGGAAGCGGGAGAATTTGTGAAGATTGCAAATGGATTATTGATCAACGTCGGCACAATAACT
TCAAACGATGCAAAAGCCATGAAAATCGCAGCAACCGTCGCTCATCAAACGAATACTCCTTGGGTGCTTGATCCCGTCGC
CGTAGGCGCACTCGGGTTTAGAACGGAAACGACTAAGAAATTACTCGACTTAAAACCGACTGTAATTAGAGGGAATGCCT
CCGAAATTCTTACGTTAGCCGGAATAGCAGGCAAAGGGAAAGGAGTCGATTCGACGGTAAACTCCAAGGATGCACTGCCC
TATGCACAAGAGCTTTCCGGAAAAACCGGCGCGGTTGTCGCCGTAAGCGGCGAAGTGGATTACGTAACAAACGGAAAGGA
GACCATCGAGATCTACGGAGGAGATCCGATTATGACCAAGGTAACCGGCGTCGGTTGTTCTTTGGGTGCTTTGATCGCTT
CTTTTTTAGGGATTCAAAAAGATCCGTTACGAGCATCCGCATCCGCTTCAGCGGTATTTGCGATTGCAGGTTCTCGTTCA
GCGAAAAAATCGAACGGTCCCGGAAGTTTTGCGATAAATTTTATAGATCAATTAAGCCAACTTTCGATCGAGTAA

Upstream 100 bases:

>100_bases
AAGTAAAATTATCTACAAACGAATTCGAAGTTTTTTCGACATAAAAAAAGGATGAAAGTTCTTTACAAGATTTCATTTTC
TTTTTTAAAACTACAGTACT

Downstream 100 bases:

>100_bases
ATAGAACTATTATTTCAAACCGTTCTACGGAAAAAAACGATAGATAAATTCATAAAACTTAAAATTCAATCCAAAGCTCT
TCTATAAATTCATAATGATT

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MPKHSALERAWPAKEIVEDLLELRKRSPLTHIMTNIVVTNWTANVLLAVGASPAMVIAEEEAGEFVKIANGLLINVGTIT
SNDAKAMKIAATVAHQTNTPWVLDPVAVGALGFRTETTKKLLDLKPTVIRGNASEILTLAGIAGKGKGVDSTVNSKDALP
YAQELSGKTGAVVAVSGEVDYVTNGKETIEIYGGDPIMTKVTGVGCSLGALIASFLGIQKDPLRASASASAVFAIAGSRS
AKKSNGPGSFAINFIDQLSQLSIE

Sequences:

>Translated_264_residues
MPKHSALERAWPAKEIVEDLLELRKRSPLTHIMTNIVVTNWTANVLLAVGASPAMVIAEEEAGEFVKIANGLLINVGTIT
SNDAKAMKIAATVAHQTNTPWVLDPVAVGALGFRTETTKKLLDLKPTVIRGNASEILTLAGIAGKGKGVDSTVNSKDALP
YAQELSGKTGAVVAVSGEVDYVTNGKETIEIYGGDPIMTKVTGVGCSLGALIASFLGIQKDPLRASASASAVFAIAGSRS
AKKSNGPGSFAINFIDQLSQLSIE
>Mature_263_residues
PKHSALERAWPAKEIVEDLLELRKRSPLTHIMTNIVVTNWTANVLLAVGASPAMVIAEEEAGEFVKIANGLLINVGTITS
NDAKAMKIAATVAHQTNTPWVLDPVAVGALGFRTETTKKLLDLKPTVIRGNASEILTLAGIAGKGKGVDSTVNSKDALPY
AQELSGKTGAVVAVSGEVDYVTNGKETIEIYGGDPIMTKVTGVGCSLGALIASFLGIQKDPLRASASASAVFAIAGSRSA
KKSNGPGSFAINFIDQLSQLSIE

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=242, Percent_Identity=53.7190082644628, Blast_Score=251, Evalue=5e-68,
Organism=Saccharomyces cerevisiae, GI6325042, Length=293, Percent_Identity=31.740614334471, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_LEPBJ (Q04V49)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_799979.1
- ProteinModelPortal:   Q04V49
- SMR:   Q04V49
- STRING:   Q04V49
- GeneID:   4409425
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_0526
- eggNOG:   COG2145
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- PhylomeDB:   Q04V49
- ProtClustDB:   PRK09355
- BioCyc:   LBOR355277:LBJ_0526-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 27495; Mature: 27364

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 130-130 BINDING 176-176 BINDING 203-203

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKHSALERAWPAKEIVEDLLELRKRSPLTHIMTNIVVTNWTANVLLAVGASPAMVIAEE
CCCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCEEEEEECCCCEEEEEEC
EAGEFVKIANGLLINVGTITSNDAKAMKIAATVAHQTNTPWVLDPVAVGALGFRTETTKK
CCCCEEEECCCEEEEEEEEECCCCHHEEEEEEEHHCCCCCEEECHHHHHHCCCCCHHHHH
LLDLKPTVIRGNASEILTLAGIAGKGKGVDSTVNSKDALPYAQELSGKTGAVVAVSGEVD
HHCCCCEEEECCHHHEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCEE
YVTNGKETIEIYGGDPIMTKVTGVGCSLGALIASFLGIQKDPLRASASASAVFAIAGSRS
EEECCCEEEEEECCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCC
AKKSNGPGSFAINFIDQLSQLSIE
CCCCCCCCCEEHHHHHHHHHHCCC
>Mature Secondary Structure 
PKHSALERAWPAKEIVEDLLELRKRSPLTHIMTNIVVTNWTANVLLAVGASPAMVIAEE
CCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHEEECCCCEEEEEECCCCEEEEEEC
EAGEFVKIANGLLINVGTITSNDAKAMKIAATVAHQTNTPWVLDPVAVGALGFRTETTKK
CCCCEEEECCCEEEEEEEEECCCCHHEEEEEEEHHCCCCCEEECHHHHHHCCCCCHHHHH
LLDLKPTVIRGNASEILTLAGIAGKGKGVDSTVNSKDALPYAQELSGKTGAVVAVSGEVD
HHCCCCEEEECCHHHEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCEE
YVTNGKETIEIYGGDPIMTKVTGVGCSLGALIASFLGIQKDPLRASASASAVFAIAGSRS
EEECCCEEEEEECCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCC
AKKSNGPGSFAINFIDQLSQLSIE
CCCCCCCCCEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA